##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 490618 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.024884941033555 31.0 28.0 31.0 16.0 34.0 2 28.488145563350713 31.0 28.0 33.0 22.0 34.0 3 28.814890607356435 31.0 28.0 33.0 23.0 34.0 4 32.54441133427636 35.0 32.0 37.0 26.0 37.0 5 32.22749267250692 35.0 32.0 37.0 24.0 37.0 6 32.3543265840226 35.0 33.0 37.0 25.0 37.0 7 32.15056520551631 35.0 33.0 37.0 20.0 37.0 8 32.1123338320241 35.0 33.0 37.0 19.0 37.0 9 33.27334708469726 37.0 33.0 39.0 18.0 39.0 10 33.11835684789388 37.0 33.0 39.0 17.0 39.0 11 33.38256036264467 37.0 33.0 39.0 19.0 39.0 12 33.16453126464989 37.0 33.0 39.0 17.0 39.0 13 33.31223681153158 37.0 33.0 39.0 19.0 39.0 14 33.884767782674096 38.0 33.0 40.0 17.0 41.0 15 34.05018364593227 38.0 33.0 40.0 17.0 41.0 16 34.01808331532883 38.0 33.0 40.0 17.0 41.0 17 33.96680309324159 38.0 33.0 40.0 17.0 41.0 18 33.94603744664892 38.0 33.0 40.0 17.0 41.0 19 33.964173756364424 39.0 33.0 40.0 18.0 41.0 20 33.96300380336637 39.0 33.0 40.0 18.0 41.0 21 33.85609170474789 39.0 33.0 40.0 11.0 41.0 22 33.79536625235927 39.0 33.0 40.0 10.0 41.0 23 33.42560199585013 39.0 33.0 40.0 8.0 41.0 24 33.51828102515603 39.0 33.0 40.0 2.0 41.0 25 33.30426115633752 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8108135553427367 1101 2 0.8470774560030492 1101 3 0.9824567460657398 1101 4 0.7026696080792618 1101 5 0.7663197451369257 1101 6 0.7707827183387153 1101 7 0.8952131232833338 1101 8 0.9649546009199597 1101 9 1.2231023992353869 1101 10 1.3147939573915224 1101 11 1.087213674997237 1101 12 1.0708579351709133 1101 13 0.938075012459926 1101 14 1.5463686859087176 1101 15 1.228887059716378 1101 16 1.0523147025584265 1101 17 1.4365526563631832 1101 18 1.6380460759933655 1101 19 1.370844906367033 1101 20 1.288080599137686 1101 21 1.1117708486693942 1101 22 1.2389969659895712 1101 23 1.5795450254303987 1101 24 1.27596127113285 1101 25 1.2961458052415509 1102 1 -0.1428409040466434 1102 2 -0.04686791468403584 1102 3 0.033848960478344736 1102 4 -0.10782151507116566 1102 5 -0.010017023447083773 1102 6 -0.44786004501517596 1102 7 -0.26073033852248884 1102 8 0.19867118170536457 1102 9 0.03271631972440758 1102 10 0.10976852403133819 1102 11 -0.0465266288790005 1102 12 0.17722565411297353 1102 13 0.0262973254061194 1102 14 0.20082255828952356 1102 15 -0.20685784533608853 1102 16 -0.1851796425187544 1102 17 0.003242126621046282 1102 18 0.151953048375276 1102 19 0.1858047977925139 1102 20 0.0774650676852815 1102 21 0.1823373891807094 1102 22 0.23099456057850176 1102 23 0.4263862682996802 1102 24 0.33658756073110396 1102 25 0.18938500020122007 1103 1 0.015453074701881064 1103 2 -0.08146059473126144 1103 3 0.07603707539373161 1103 4 -0.22745945289091907 1103 5 0.24966420441596426 1103 6 -0.20964674316551424 1103 7 0.11884463196157213 1103 8 0.1255798790686029 1103 9 0.5357859773395219 1103 10 0.31353672552680933 1103 11 0.05461465408045996 1103 12 0.29102927473477536 1103 13 0.18220051312748353 1103 14 0.4212574264516675 1103 15 0.3151020129874027 1103 16 0.4734094956158401 1103 17 0.5595388601905071 1103 18 0.4435311672261193 1103 19 0.5744608387212864 1103 20 0.3771994242378227 1103 21 0.4972626154695732 1103 22 0.22187413554898683 1103 23 0.5042001211135343 1103 24 0.20725855640209545 1103 25 0.34153966371952293 1104 1 -0.02545865584399465 1104 2 0.06128040410729696 1104 3 0.0037954958350781 1104 4 -0.12292983616618613 1104 5 0.08607535424174984 1104 6 -0.2657443041411227 1104 7 -0.2278452635088115 1104 8 -0.06521570745868743 1104 9 0.31592535016181955 1104 10 0.34980269532936603 1104 11 0.19050315582261845 1104 12 0.020210459403763537 1104 13 -0.3266845042941142 1104 14 0.3583944763935918 1104 15 0.1626049165878669 1104 16 0.041963502584479784 1104 17 0.1465888021185009 1104 18 0.19974487222031456 1104 19 0.3751867388374279 1104 20 0.30910999334351175 1104 21 0.35646702894227644 1104 22 0.3138311622621117 1104 23 0.37211533597996294 1104 24 0.0047324239979147364 1104 25 0.628850929689321 1105 1 0.8147040265310466 1105 2 0.8271472516517875 1105 3 0.7471400936578156 1105 4 0.5356040575654077 1105 5 0.7598694684450962 1105 6 0.3447923673570301 1105 7 0.3819633209203559 1105 8 0.29871208676048866 1105 9 0.7585471096023966 1105 10 0.8517574947151374 1105 11 0.6968372441593687 1105 12 0.7512166057506064 1105 13 0.5784676874363726 1105 14 0.7948227853878223 1105 15 0.7474310975760474 1105 16 0.7222135015751121 1105 17 0.786121103748421 1105 18 0.709508102612098 1105 19 0.797535108985592 1105 20 0.6945102999206512 1105 21 0.7096350151826414 1105 22 0.9546460276198374 1105 23 1.3577191301076255 1105 24 0.8878508976259596 1105 25 0.9814792432593293 1106 1 0.6835129093054171 1106 2 0.7938920889074588 1106 3 0.6565354268341927 1106 4 0.6808698266074842 1106 5 0.937202715847782 1106 6 -0.2215542076268946 1106 7 -0.20554323896432436 1106 8 -0.15296047010171065 1106 9 0.2147060357147481 1106 10 0.1283156563954151 1106 11 -0.021002880106877342 1106 12 0.056059543368903064 1106 13 -0.2467033299080228 1106 14 0.05689198674570406 1106 15 -0.07229866805685958 1106 16 -0.3024661327285756 1106 17 0.06391429935595738 1106 18 -0.19042915924271853 1106 19 0.21953002417072298 1106 20 0.11615272405945376 1106 21 0.04186966373723067 1106 22 -0.03664297163440722 1106 23 0.5069175417934915 1106 24 0.186698856926391 1106 25 0.24880818974514085 1107 1 0.811530358074247 1107 2 0.7447689134654603 1107 3 0.7991440230908431 1107 4 0.7709792668592712 1107 5 0.8668773191700652 1107 6 0.25108705855189584 1107 7 0.5193130160365129 1107 8 0.7342645004581456 1107 9 0.7439826986509956 1107 10 0.561565609211435 1107 11 0.4113671911608705 1107 12 0.8849597977636563 1107 13 0.7125283819799435 1107 14 0.8633924771932726 1107 15 0.9694345656806149 1107 16 0.7033275284027241 1107 17 0.6246832786136167 1107 18 1.1063726972807686 1107 19 0.9393515491037974 1107 20 0.5648457942612382 1107 21 1.1137926701143783 1107 22 0.9554221449166675 1107 23 1.0735014482017462 1107 24 1.1171922019914788 1107 25 1.2493265489654206 1108 1 1.2228178850327929 1108 2 1.2684183398934366 1108 3 1.2072719422218192 1108 4 0.8824679078838997 1108 5 -0.08701860430074504 1108 6 0.46388029476830894 1108 7 0.9712255843425268 1108 8 1.0882715659128337 1108 9 1.3970450572426714 1108 10 1.3817167093847615 1108 11 1.0295944765179144 1108 12 1.0838258849042646 1108 13 1.0334918771307144 1108 14 1.351221106839077 1108 15 1.2377008830438996 1108 16 1.176052756309943 1108 17 1.391782605146119 1108 18 1.4428047749743058 1108 19 1.303819192232183 1108 20 1.120641716408926 1108 21 1.1772666509820766 1108 22 1.1955020612955565 1108 23 1.4748417676026335 1108 24 1.374873568508633 1108 25 1.410567105204592 1109 1 0.9213032061655078 1109 2 -0.1641073957829704 1109 3 0.6128176772050082 1109 4 0.9295373335537178 1109 5 -0.3558134467672076 1109 6 0.38880490743337504 1109 7 0.8542521692096017 1109 8 0.8751489705088602 1109 9 1.1569078003018376 1109 10 -0.6456584249260047 1109 11 0.5939076719010856 1109 12 1.182491442423938 1109 13 0.9550751844684129 1109 14 1.4471484577107958 1109 15 1.4077400497972192 1109 16 1.3017399075352074 1109 17 1.3023229676985437 1109 18 1.3723947371939857 1109 19 1.30991950071369 1109 20 1.3854159010125784 1109 21 1.4791279382858136 1109 22 1.4150161162676511 1109 23 1.5300911523726626 1109 24 1.5323169846813869 1109 25 1.6039191553934913 1110 1 0.7904530747018796 1110 2 -0.04599261443569702 1110 3 0.4047727075776386 1110 4 0.5947901366000465 1110 5 0.6890730714110376 1110 6 0.7202383143057531 1110 7 0.6582534989566469 1110 8 0.8119920301359258 1110 9 1.174209622659717 1110 10 1.1563528174808368 1110 11 0.5615933076108419 1110 12 0.8865547262947047 1110 13 1.0309689860338906 1110 14 1.4419881325272002 1110 15 1.008944377519427 1110 16 0.8259957025123867 1110 17 1.024727694344861 1110 18 0.9544507074560045 1110 19 1.0729419553058506 1110 20 0.9972733158634384 1110 21 1.1092905300836904 1110 22 1.2183027069775605 1110 23 1.5727140784205957 1110 24 1.5112404940047242 1110 25 1.6020076440150888 1111 1 0.2230895439237166 1111 2 0.2819540394150799 1111 3 0.37313347492915483 1111 4 0.1918204541938664 1111 5 0.2057036933822829 1111 6 -0.13914732388560225 1111 7 0.1600757585585484 1111 8 0.4086518883601258 1111 9 0.45762105283313304 1111 10 0.6180953782561929 1111 11 0.12081674467279413 1111 12 0.1944264872783279 1111 13 -0.0061270130049209115 1111 14 0.23528181669556858 1111 15 0.32172222204663825 1111 16 0.46937349097007797 1111 17 0.1953692899233772 1111 18 0.1335427816279804 1111 19 0.0883110129373108 1111 20 0.04197875060251022 1111 21 0.29607213927909726 1111 22 0.3045396407754666 1111 23 0.6450537796501195 1111 24 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0.6720620938074049 2202 21 0.5649829861500848 2202 22 0.5974268067933366 2202 23 0.7621454680682547 2202 24 0.8681263619064836 2202 25 0.9626458435888878 2203 1 0.23436841861722613 2203 2 0.37109672449272324 2203 3 0.32735982671648145 2203 4 0.0011119756805086922 2203 5 0.22206279348122138 2203 6 0.27342203990327363 2203 7 0.30579348557709096 2203 8 0.5066645351532628 2203 9 0.610918252471798 2203 10 0.41119986485661997 2203 11 0.3860079521404245 2203 12 0.3604737191792182 2203 13 0.18969610395640757 2203 14 0.8809135111077495 2203 15 0.7459662105182687 2203 16 0.7303318942049017 2203 17 0.7393448566631733 2203 18 0.5675170578786819 2203 19 0.7160393219664343 2203 20 0.9327990715041352 2203 21 0.9287000052402945 2203 22 0.6340875394290535 2203 23 0.7465722551523299 2203 24 0.5678846586948652 2203 25 0.6854990993456198 2204 1 0.16003290663465464 2204 2 0.383413354848571 2204 3 -0.07595172012447549 2204 4 -0.07496645569204219 2204 5 -0.06352907289495846 2204 6 -0.32975878798344027 2204 7 -0.07544108232414004 2204 8 -0.14801956070730427 2204 9 0.4025086664151516 2204 10 0.35388686558283666 2204 11 -0.03104360922485938 2204 12 0.08465672571516336 2204 13 -0.026454949057388433 2204 14 0.44662157210992603 2204 15 0.23274907181093596 2204 16 0.15689689057381884 2204 17 -0.12228186810080643 2204 18 -0.0738918019615511 2204 19 0.11027316365125728 2204 20 0.198754046086556 2204 21 0.10492367989534301 2204 22 0.30073968176747456 2204 23 0.6401525020659093 2204 24 0.4550596768502757 2204 25 0.4950270586775076 2205 1 0.3319907844265906 2205 2 0.24548190404703973 2205 3 0.37899629785124134 2205 4 0.0190519224687975 2205 5 0.16460963526789385 2205 6 0.12666753168924316 2205 7 -0.09658133317932993 2205 8 0.17259215033971742 2205 9 0.24849162972590477 2205 10 0.27739213085884273 2205 11 0.15370788316229067 2205 12 0.33932840054244906 2205 13 -0.006079337010973518 2205 14 0.6549749157278768 2205 15 0.4780438601937931 2205 16 0.2522782038745106 2205 17 0.46509622566805575 2205 18 0.1728410439704362 2205 19 0.25191780782591877 2205 20 0.41492951057109906 2205 21 0.43203347934533554 2205 22 0.24448775340205486 2205 23 0.5905971998994346 2205 24 0.3274803623422642 2205 25 0.3056421234035085 2206 1 0.11483053210686123 2206 2 0.2679168626737187 2206 3 0.3716683554286817 2206 4 0.2982067769037471 2206 5 0.4933732476662911 2206 6 0.410187245475818 2206 7 0.553641486483194 2206 8 0.684077039400627 2206 9 0.9069851909699267 2206 10 0.7632527587290809 2206 11 0.5509558511097339 2206 12 0.48032591082039744 2206 13 0.6398348512664143 2206 14 0.7012923762113843 2206 15 0.6595974258314428 2206 16 0.6902509110811081 2206 17 0.6529420930432863 2206 18 0.45042282297099234 2206 19 0.3191965311633993 2206 20 0.6804431988118722 2206 21 0.6303338256049997 2206 22 0.5364547384323402 2206 23 0.9686387405980241 2206 24 0.7997115926624758 2206 25 0.3739774094600179 2207 1 -0.9731938474816282 2207 2 0.025275758346555932 2207 3 0.4265597234773537 2207 4 0.4380457735899128 2207 5 0.3485608362394288 2207 6 0.5681697492851256 2207 7 0.8186123439244071 2207 8 0.7628911450384095 2207 9 1.0162737822175743 2207 10 0.9781418358636316 2207 11 0.7851605309678007 2207 12 0.8250885081842512 2207 13 0.4700634631855607 2207 14 -4.959840134523944 2207 15 -1.8327493226688105 2207 16 -0.3645405624561704 2207 17 -0.25759238255049155 2207 18 0.5214638036953474 2207 19 0.3740252986515955 2207 20 0.6250217238893896 2207 21 0.6619257978156767 2207 22 0.6541331367104277 2207 23 0.8552744532854248 2207 24 0.6075198804439097 2207 25 0.6787928576800297 2208 1 0.6959316540935028 2208 2 1.1537838709479864 2208 3 0.7820730683862465 2208 4 0.6637652644207535 2208 5 0.8248911513820403 2208 6 0.8212976747354261 2208 7 0.8064541239188898 2208 8 1.0398616118285986 2208 9 1.0335244707301925 2208 10 1.1250154641794623 2208 11 0.8709866878437964 2208 12 1.005103968157293 2208 13 0.8167203411255741 2208 14 1.2663959835467438 2208 15 1.0474320018391339 2208 16 1.1534501071781875 2208 17 0.7000946811811133 2208 18 1.013191941234723 2208 19 0.7394672091624415 2208 20 0.6790189814921561 2208 21 0.8337373725965023 2208 22 0.9151891538640058 2208 23 1.3379674407504183 2208 24 1.0185143296143906 2208 25 0.8247575328069559 2209 1 -0.12671661007343715 2209 2 -0.12797300251127552 2209 3 0.4952523671442357 2209 4 0.697590848919944 2209 5 0.5653248348653293 2209 6 0.4261346116299265 2209 7 0.5254509364552007 2209 8 0.685589939430777 2209 9 0.6405478821014299 2209 10 0.6017493345140679 2209 11 0.7053524139731522 2209 12 0.6852781010258511 2209 13 0.8697256640329201 2209 14 1.028148774540199 2209 15 1.0620503697949175 2209 16 0.9336878323026241 2209 17 1.0895187394661647 2209 18 1.211874560921629 2209 19 1.210057753543552 2209 20 0.8335206851365484 2209 21 0.9738052043361023 2209 22 1.1317835922894304 2209 23 1.3092135349297607 2209 24 1.2769265644503847 2209 25 1.0978273900776685 2210 1 -0.13178815712019087 2210 2 -0.1318455391281823 2210 3 0.4007020311964098 2210 4 0.5619902191934827 2210 5 1.0713494054424828 2210 6 1.113164776651999 2210 7 1.2710259835441207 2210 8 1.1396873783557453 2210 9 1.578472033798036 2210 10 1.3924004266959003 2210 11 1.1036023928885612 2210 12 0.9134344643555323 2210 13 0.8231329340000286 2210 14 1.1604433403381762 2210 15 0.9779762644844112 2210 16 0.9895985460863272 2210 17 0.731124260817829 2210 18 0.7386266904653596 2210 19 0.9508437871102231 2210 20 1.0937734191052257 2210 21 1.2436626724983668 2210 22 1.3841281988509522 2210 23 1.1667393284132146 2210 24 1.2961022974913448 2210 25 1.234674821974437 2211 1 -1.034782169096239 2211 2 -0.2611868564934525 2211 3 0.4929176000586857 2211 4 0.5248644706904884 2211 5 0.8773374293945793 2211 6 0.6652605850352273 2211 7 0.5967744865381306 2211 8 0.7720227076970616 2211 9 0.9281726326004289 2211 10 0.7373106063366208 2211 11 0.5682730515711967 2211 12 0.6954703924992458 2211 13 0.5704383232215804 2211 14 0.9017607056076429 2211 15 0.7121080010113587 2211 16 0.7225326778228478 2211 17 0.81016617698549 2211 18 0.6703632174114631 2211 19 0.5664126493856685 2211 20 0.8422764131172045 2211 21 0.7801896186061512 2211 22 0.5913138275934671 2211 23 0.7894938194311791 2211 24 0.7990963094673944 2211 25 0.9508424866451008 2212 1 1.2316082439939322 2212 2 0.19315465060641657 2212 3 0.6689032713017511 2212 4 0.969172812182407 2212 5 1.065244504977251 2212 6 1.0002210822916666 2212 7 1.0287667757240158 2212 8 1.1255210342378987 2212 9 1.301511126714864 2212 10 0.8659855757893524 2212 11 0.8777180399214899 2212 12 1.3143816194749505 2212 13 1.1381212084017918 2212 14 1.3369056248945412 2212 15 1.2837965630507782 2212 16 0.815085215333383 2212 17 1.1605437488379806 2212 18 1.0890680131431978 2212 19 1.0728810813430485 2212 20 1.2512941191500033 2212 21 1.3904049294293728 2212 22 1.5979423955526073 2212 23 1.8426620077693343 2212 24 1.6831548084390349 2212 25 1.2695905418654547 2213 1 0.7554090302744569 2213 2 0.45239616513086034 2213 3 0.19934870567587026 2213 4 -0.670648524766321 2213 5 -1.3607724099080478 2213 6 0.0789527717074634 2213 7 -0.002947784737010295 2213 8 -0.14833496837171012 2213 9 -0.22897848072252458 2213 10 0.19530935414930894 2213 11 -0.11722244537057946 2213 12 -0.2543907431383303 2213 13 0.11914038290917972 2213 14 -6.777213785089252 2213 15 -2.7074180942511994 2213 16 -1.3215828444837427 2213 17 -0.7651962349199195 2213 18 -4.656456066273176 2213 19 -2.3024030703812315 2213 20 -3.8973601314879573 2213 21 -1.3574450316643478 2213 22 -1.108870789766982 2213 23 -3.8291236373442743 2213 24 -1.6722735513471676 2213 25 -0.9090827031897106 2214 1 0.013201412400853485 2214 2 -0.32754159412678874 2214 3 -1.7923758468978939 2214 4 -2.108714070393777 2214 5 -0.6964470186346077 2214 6 -0.6957572945793089 2214 7 -0.7809135787980495 2214 8 -0.7666207388372293 2214 9 -5.411403222741075 2214 10 -1.9100158972148087 2214 11 -1.0710485872386357 2214 12 -1.473768562594799 2214 13 -1.4411019249140224 2214 14 -2.750857893583259 2214 15 -2.1950531351085054 2214 16 -1.3913749089781575 2214 17 -1.7753358795502407 2214 18 -1.1533591544878945 2214 19 -4.353988016138008 2214 20 -2.131842485897181 2214 21 -4.42714634416506 2214 22 -6.443242729250532 2214 23 -5.909664141093465 2214 24 -5.618644795065428 2214 25 -3.6393942472214427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19224.0 3 12.0 4 340.0 5 2294.0 6 2840.0 7 2263.0 8 1655.0 9 1446.0 10 1309.0 11 1176.0 12 1054.0 13 1089.0 14 1023.0 15 1033.0 16 1030.0 17 1165.0 18 1307.0 19 1561.0 20 1950.0 21 2564.0 22 3296.0 23 4085.0 24 4770.0 25 5323.0 26 6263.0 27 6995.0 28 7861.0 29 9542.0 30 11702.0 31 14653.0 32 18507.0 33 23741.0 34 30920.0 35 40542.0 36 54104.0 37 79433.0 38 122546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.801659703636005 19.993757751811476 9.469041712905542 29.735540831646972 2 11.809658963474062 20.998030364199117 43.64068623253121 23.551624439795617 3 19.954857507539042 24.238500796212435 28.61348932708325 27.193152369165276 4 11.34734854740482 17.764090142659956 38.60076213078556 32.287799179149665 5 11.909407281912648 39.83319164204908 35.783878997681974 12.4735220783563 6 31.4328089519874 39.11421148456519 17.45248859521715 12.000490968230263 7 27.94458029707804 31.182980218630814 21.117627951730437 19.75481153256071 8 22.932181054825165 43.11544237075174 18.973164577651108 14.979211996771985 9 25.536892388601593 16.201063671115996 19.77557887940634 38.48646506087607 10 15.089085497614874 31.134871773963386 33.775023443552 20.001019284869738 11 33.915267883531556 22.504254950520274 25.618572986414733 17.961904179533434 12 24.65129362269389 24.79193801862621 32.51127703073182 18.045491327948078 13 30.343974334410884 23.353810907875374 25.27078093343497 21.031433824278768 14 19.15763363917118 25.665697069586606 26.86531847796376 28.311350813278445 15 21.362836800792483 35.89232973712264 22.452502909649027 20.29233055243585 16 19.378561935899423 29.56641201457795 32.10389642155384 18.95112962796879 17 19.42433285228579 31.597689341127932 29.415241869073533 19.56273593751274 18 19.139954586406088 29.017411262082092 33.83903856894183 18.003595582569986 19 23.501451260476795 27.27615530117731 27.132659883246912 22.089733555098977 20 22.87184169975459 29.64888751192388 29.110580254865354 18.36869053345617 21 20.717340170968043 27.890130406956125 27.362224785882294 24.030304636193538 22 21.564842708583868 31.741191721461504 27.24971362648741 19.44425194346722 23 20.249766620874084 31.00395827303523 28.682804136823354 20.063470969267332 24 23.284496569604617 27.749310048225283 30.235174699458227 18.731018682711866 25 21.079944070539604 29.85316478400711 29.517058077771303 19.549833067681984 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 245.0 2 246.5 3 248.0 4 248.0 5 248.0 6 319.0 7 390.0 8 390.0 9 390.0 10 681.5 11 973.0 12 973.0 13 973.0 14 1484.5 15 1996.0 16 1996.0 17 1996.0 18 3636.5 19 5277.0 20 5277.0 21 5277.0 22 8887.0 23 12497.0 24 12497.0 25 12497.0 26 19408.5 27 26320.0 28 26320.0 29 26320.0 30 37108.0 31 47896.0 32 47896.0 33 47896.0 34 52937.5 35 57979.0 36 57979.0 37 57979.0 38 60272.0 39 62565.0 40 62565.0 41 62565.0 42 64250.5 43 65936.0 44 65936.0 45 65936.0 46 67506.5 47 69077.0 48 69077.0 49 69077.0 50 62711.5 51 56346.0 52 56346.0 53 56346.0 54 45867.5 55 35389.0 56 35389.0 57 35389.0 58 29229.0 59 23069.0 60 23069.0 61 23069.0 62 18071.5 63 13074.0 64 13074.0 65 13074.0 66 9899.5 67 6725.0 68 6725.0 69 6725.0 70 4789.0 71 2853.0 72 2853.0 73 2853.0 74 2007.0 75 1161.0 76 1161.0 77 1161.0 78 792.5 79 424.0 80 424.0 81 424.0 82 277.5 83 131.0 84 131.0 85 131.0 86 83.5 87 36.0 88 36.0 89 36.0 90 23.0 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08356807128967955 2 0.03505782502884118 3 0.03485400046472 4 0.030777509182296617 5 0.023439824873934506 6 0.3644383206486513 7 0.2276720381233465 8 0.2350097224317086 9 0.2407168102271013 10 0.01589831600145123 11 0.0026497193335752053 12 0.003872666718302223 13 0.0 14 0.07745333436604446 15 0.0018344210770905266 16 0.0028535438976963748 17 0.004484140410665732 18 0.0032611930259387138 19 0.0020382456412116963 20 0.0012229473847270177 21 0.0 22 0.0 23 0.0 24 8.152982564846784E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 490618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.8574379696808 #Duplication Level Percentage of deduplicated Percentage of total 1 93.7044540697485 72.01884266148501 2 4.371850948856141 6.72018526228802 3 0.778213405323862 1.794344655805584 4 0.32148033785368696 0.988326205002471 5 0.18442277224004872 0.7087130888818057 6 0.10872892444164048 0.5013975939470098 7 0.07110025713267479 0.38252085215420356 8 0.05540684863471854 0.3406742745630699 9 0.0501476385680334 0.346879711851174 >10 0.2817708705329047 4.083394457211729 >50 0.03754328679982348 2.0282269559036914 >100 0.027691500353194842 4.36874046235978 >500 0.004526495250041383 2.2748228864338986 >1k 0.002662644264730225 3.4429309321126222 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 2704 0.5511416213836426 No Hit GTCCTACAGTGGACATTTCTAAATT 2692 0.5486957266141886 No Hit GTCCTACAGTGTGCATTTCTCATTT 1788 0.3644383206486513 No Hit CTTTAGGACGTGAAATATGGCGAGG 1761 0.35893505741737974 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1666 0.3395717238258686 No Hit CTGTAGGACCTGGAATATGGCGAGA 1622 0.33060344300453715 No Hit GTATCAACGCAGAGTACTTTTTTTT 1206 0.24581242433013056 No Hit GATATACACTGTTCTACAAATCCCG 1172 0.2388823891500108 No Hit GTACATGGGAAGCAGTGGTATCAAC 1131 0.23052558202104287 No Hit CTGAAGGACCTGGAATATGGCGAGA 1082 0.22053817837910553 No Hit CCCATGTACTCTGCGTTGATACCAC 921 0.18772242355559723 No Hit GTGTATATCAATGAGTTACAATGAA 871 0.17753119534953873 No Hit GGTATCAACGCAGAGTACTTTTTTT 829 0.1689705636564496 No Hit GTGTATATCAATGAGTTACAATGAG 781 0.15918698457863348 No Hit GATATACACTGTTCTACAATGCCGG 729 0.14858810724433266 No Hit GTTCTACAGTGTGGTTTTTATCATT 700 0.14267719488481873 No Hit CTGTAGAACATATTAGATGAGTGAG 640 0.13044772103754856 No Hit ACCTGGAATATGGCGAGAAAACTGA 619 0.126167405191004 No Hit CTGTAGGACATGGAATATGGCAAGA 591 0.12046031739561126 No Hit TATCAACGCAGAGTACTTTTTTTTT 584 0.11903354544676306 No Hit TTGTAGAACAGTGTATATCAATGAG 567 0.11556852785670317 No Hit GTCCTTCAGTGTGCATTTCTCATTT 562 0.11454940503609734 No Hit GAATATGGCAAGAAAACTGAAAATC 553 0.11271498395900681 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 549 0.11189968570252214 No Hit ACAGTGGACATTTCTAAATTTTCCA 546 0.11128821201015862 No Hit GAGTACATGGGAAGCAGTGGTATCA 543 0.11067673831779509 No Hit GACCTGGAATATGGCGAGAAAACTG 531 0.10823084354834107 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAACG 25 0.005894748 19.072725 3 CCAACGA 105 0.0 18.04787 19 TCCAACG 185 0.0 16.389418 18 GTCTTAG 35 0.002118516 16.341345 1 GGACCTA 55 1.0861881E-5 15.603356 6 TCTAGGA 160 0.0 15.499769 2 TAGGACC 570 0.0 15.055871 4 ACTGTGC 110 1.0913936E-11 14.683793 8 AGCAACG 40 0.0051332777 14.307479 2 TAGGCTG 60 2.4728994E-5 14.303076 5 GTTCTAG 75 9.27017E-7 13.980928 1 TGTAGGA 1375 0.0 13.596441 2 CCAACGT 70 7.4369636E-6 13.535903 19 TGTCGAG 70 7.4369636E-6 13.535903 18 CTAGGAC 185 0.0 13.402455 3 CTGTAGG 1325 0.0 13.381404 1 ATCCCGT 115 3.8562575E-10 13.192204 10 GAACAGT 155 0.0 12.918908 6 AGGACCT 955 0.0 12.880258 5 GATATAC 395 0.0 12.790379 1 >>END_MODULE