FastQCFastQC Report
Thu 26 May 2016
SRR1926503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420678
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC16920.40220786444739204No Hit
GTACATGGGAAGCAGTGGTATCAAC16800.3993553264016659No Hit
GTCCTACAGTGGACATTTCTAAATT8710.20704671981895892No Hit
GAGTACATGGGAAGCAGTGGTATCA8640.2053827392922853No Hit
CATGTACTCTGCGTTGATACCACTG6540.15546332349207706No Hit
CTGTAGGACGTGGAATATGGCAAGA6150.14619257484346698No Hit
GCGTTGATACCACTGCTTCCCATGT6030.1433400367977408No Hit
GTCCTAAAGTGTGTATTTCTCATTT5030.11956888641668924No Hit
GTATCAACGCAGAGTACATGGGAAG4970.11814261739382616No Hit
ACGCAGAGTACATGGGAAGCAGTGG4860.11552779085191049No Hit
ACTCTGCGTTGATACCACTGCTTCC4310.10245365814233214No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC303.0792324E-421.7673383
GTGCTAA405.583731E-419.927861
GTGTAGG501.165307E-418.5993371
TAGGACC1850.016.7289734
TCTACAC802.4056135E-816.3255043
CTTTAGG2300.016.1733381
TGTAGGA3950.016.1658062
TTTAGGA2550.015.9837122
TCTAGGA851.3175177E-615.9837122
GATATAC2600.015.84009551
CTCCTGA604.6883046E-415.4994471
TTAGGAC2400.015.4185313
TTCAGGA550.00405272114.821262
TATAGGA550.00405272114.821262
GTCCTAC4800.014.669121
AGGACCT3400.014.6166275
GGACCTG3050.014.5895996
TATAGTG507.804271E-414.4948225
GTATAAA550.004703437414.49299051
CTGTAGG3700.014.3624231
Produced by FastQC (version 0.11.5)