##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926503_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 420678 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.95569057568972 26.0 2.0 30.0 2.0 31.0 2 15.71056960430543 16.0 2.0 30.0 2.0 31.0 3 19.01518025663334 19.0 10.0 30.0 2.0 31.0 4 21.032561721791964 27.0 2.0 35.0 2.0 35.0 5 21.22091956318134 27.0 2.0 35.0 2.0 35.0 6 22.17845240302559 29.0 2.0 35.0 2.0 36.0 7 22.049938432720513 30.0 2.0 35.0 2.0 37.0 8 22.501271756545385 30.0 2.0 35.0 2.0 37.0 9 24.86750911623617 31.0 12.0 37.0 2.0 39.0 10 25.488501894560684 31.0 11.0 37.0 2.0 39.0 11 22.722704776574957 29.0 2.0 37.0 2.0 39.0 12 23.730111867033692 30.0 2.0 37.0 2.0 39.0 13 20.57529749594702 26.0 2.0 35.0 2.0 39.0 14 23.69000993634086 30.0 2.0 37.0 2.0 40.0 15 24.731079352854202 31.0 2.0 38.0 2.0 40.0 16 25.06795458759431 31.0 2.0 38.0 2.0 40.0 17 24.384165561308173 30.0 2.0 38.0 2.0 40.0 18 24.42515415591022 30.0 2.0 37.0 2.0 40.0 19 24.79341919472851 30.0 2.0 38.0 2.0 40.0 20 25.037199473231308 31.0 2.0 38.0 2.0 40.0 21 22.929549441615677 30.0 2.0 36.0 2.0 40.0 22 23.83842986797503 30.0 2.0 37.0 2.0 40.0 23 23.789573022596855 30.0 2.0 37.0 2.0 40.0 24 22.959876675271822 30.0 2.0 38.0 2.0 40.0 25 22.74730078587423 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.62507586046415 1101 2 9.082668028318162 1101 3 5.882586923094909 1101 4 7.431445418587302 1101 5 6.937902147003971 1101 6 6.337165296815627 1101 7 6.172342410689446 1101 8 4.812372069599711 1101 9 4.981928236009015 1101 10 4.362029058832142 1101 11 6.995601543319118 1101 12 4.438360997620748 1101 13 9.157454360239779 1101 14 6.488288015169971 1101 15 5.72409142116096 1101 16 5.472442476414152 1101 17 1.8074690039299242 1101 18 4.118212958952892 1101 19 4.7267334357358095 1101 20 2.6998014696387322 1101 21 -0.30489001581619135 1101 22 3.7476723661542692 1101 23 4.561033473085686 1101 24 6.030643348714815 1101 25 6.353902202575743 1102 1 5.213776721892284 1102 2 9.952109193213873 1102 3 6.517748058256043 1102 4 7.77801719697726 1102 5 7.5027825527934695 1102 6 6.1974783844014425 1102 7 6.381993029430973 1102 8 6.6487225877684075 1102 9 5.895523058694746 1102 10 5.058955028485386 1102 11 7.696145948408979 1102 12 5.132772646577852 1102 13 9.601319417741202 1102 14 6.906080660235343 1102 15 4.804484092462722 1102 16 5.4255339976874914 1102 17 6.376099027105404 1102 18 6.471442221273065 1102 19 5.074846602030792 1102 20 5.601338484812111 1102 21 4.333638122711946 1102 22 5.590975527639248 1102 23 5.9473096548164115 1102 24 7.291411200391757 1102 25 7.2630587026413345 1103 1 4.025271166028585 1103 2 8.290961412786041 1103 3 5.301844051748009 1103 4 6.908837911751608 1103 5 6.135805275444014 1103 6 6.037722805426828 1103 7 6.059871262647828 1103 8 5.859266711125223 1103 9 4.982329500168667 1103 10 3.989261756534642 1103 11 4.861666313746305 1103 12 3.5488116208230487 1103 13 7.698022876982794 1103 14 6.095826809957089 1103 15 4.800512713018492 1103 16 4.834697012829761 1103 17 1.7217902283182625 1103 18 1.109885839887518 1103 19 3.1462426861175423 1103 20 3.2541940693333444 1103 21 5.506263707418068 1103 22 1.4014808246607124 1103 23 3.4003981973497375 1103 24 4.70686223298739 1103 25 1.4614871604962723 1104 1 5.710252602555787 1104 2 10.212227298834877 1104 3 6.6820775231174245 1104 4 7.393078594316222 1104 5 7.114250227607371 1104 6 7.226631130430398 1104 7 7.237361266931703 1104 8 7.36710442353419 1104 9 6.570805867439837 1104 10 5.351651089677578 1104 11 8.03294112454168 1104 12 5.368262213426217 1104 13 8.961841954520992 1104 14 7.0481847279922185 1104 15 6.235060406863987 1104 16 5.716581562308555 1104 17 6.101675634572725 1104 18 3.4013591370458798 1104 19 4.406029563170232 1104 20 3.5305666953507675 1104 21 3.097338960880869 1104 22 5.0974486241752075 1104 23 5.642671151797831 1104 24 6.3808769409696815 1104 25 5.343095042832413 1105 1 2.6315075870793656 1105 2 8.343808264229038 1105 3 5.519376399333925 1105 4 4.688969325193778 1105 5 1.1360357327522372 1105 6 3.6582176729138745 1105 7 4.668653269385626 1105 8 2.1809464106511136 1105 9 2.623523304209588 1105 10 2.5453939440318933 1105 11 5.451513877671822 1105 12 3.021585112954952 1105 13 3.5325211045725773 1105 14 2.792909150066336 1105 15 2.8372169975892874 1105 16 2.854766150022229 1105 17 3.6253904640532184 1105 18 1.3215283319930364 1105 19 1.1622462749183704 1105 20 0.538219507598054 1105 21 1.4554856941008012 1105 22 2.8629337612321777 1105 23 0.12337267992030476 1105 24 3.0156926181677512 1105 25 3.6733284440203313 1106 1 6.175300123886384 1106 2 10.489484562038248 1106 3 7.592313509725049 1106 4 8.801768787641635 1106 5 8.383447765138424 1106 6 7.651852536629772 1106 7 7.292706066586096 1106 8 7.01761338778519 1106 9 5.762781242750346 1106 10 5.177300457859889 1106 11 7.361547075508305 1106 12 4.5991659287302475 1106 13 4.503652625219772 1106 14 5.448518187329078 1106 15 5.800246982088094 1106 16 5.696121739433515 1106 17 5.943322950697578 1106 18 6.0212492439688745 1106 19 5.691650310551161 1106 20 5.35096441648696 1106 21 5.6001740402630915 1106 22 6.074761238928421 1106 23 6.327363073171632 1106 24 7.0128701367791635 1106 25 6.102653310463907 1107 1 3.658061440946522 1107 2 -13.024474828824696 1107 3 -5.144367318878588 1107 4 1.4852299476606632 1107 5 4.1057081797008905 1107 6 4.552120027816571 1107 7 4.439490633550118 1107 8 4.991559876515371 1107 9 3.262022102110585 1107 10 2.627913960788934 1107 11 5.385259631629324 1107 12 2.330347060338518 1107 13 3.8822015262578056 1107 14 3.199785593804908 1107 15 3.241832250198321 1107 16 3.073652479742254 1107 17 3.588085857717509 1107 18 -1.0840644146980374 1107 19 1.4994850980895258 1107 20 1.695656622425851 1107 21 -1.6922303177906421 1107 22 1.2711864358814324 1107 23 -1.5371710490597863 1107 24 1.8292183770716122 1107 25 2.259204075143341 1108 1 5.538616024004316 1108 2 10.245727191377325 1108 3 0.531636947144932 1108 4 5.742893230035111 1108 5 7.5521397612415875 1108 6 7.526679486329819 1108 7 7.605363093710128 1108 8 7.275214782442422 1108 9 6.000206254287033 1108 10 5.1514710982741825 1108 11 7.263639061356638 1108 12 2.3681950524548014 1108 13 3.3391522919377117 1108 14 4.899423176305135 1108 15 5.1745483716179095 1108 16 5.070506449478124 1108 17 0.45369315015406997 1108 18 4.062236527976463 1108 19 4.808202892205266 1108 20 4.973009742847005 1108 21 4.901819940893763 1108 22 1.6324126258945277 1108 23 4.656331693383905 1108 24 5.421576489647954 1108 25 6.040951264624805 1109 1 3.611848347236638 1109 2 -13.024474828824696 1109 3 -4.957310216802231 1109 4 2.261191598333479 1109 5 0.6225748816767087 1109 6 2.683322842973176 1109 7 3.68900722735426 1109 8 4.6712909285185695 1109 9 3.844262198342978 1109 10 3.179171175974261 1109 11 1.331005028722604 1109 12 1.329869489129237 1109 13 3.0343348871203055 1109 14 3.588894815776772 1109 15 3.777656374725911 1109 16 3.530937684909361 1109 17 4.04102803748896 1109 18 4.143724484464418 1109 19 2.2921384011407753 1109 20 1.5606942805315427 1109 21 0.9858525749263976 1109 22 3.244630963112865 1109 23 3.7621009241531382 1109 24 4.471343389414855 1109 25 4.8996342483077875 1110 1 3.3357494461377364 1110 2 9.114261434911567 1110 3 6.65252994303793 1110 4 7.6389138760557564 1110 5 8.232265191367016 1110 6 3.8909402255905583 1110 7 6.734511722858755 1110 8 7.118843376071016 1110 9 5.58612034020112 1110 10 5.4147356115386955 1110 11 3.4139470866646633 1110 12 5.952097261357011 1110 13 10.226542679328098 1110 14 7.889793916675874 1110 15 7.024559471629008 1110 16 6.884733888705565 1110 17 7.519974091435014 1110 18 7.176566642306575 1110 19 6.031424115426489 1110 20 6.511818156655419 1110 21 4.451062365136188 1110 22 6.334516077997979 1110 23 7.3024355894878035 1110 24 8.089200532271999 1110 25 8.624659223332763 1111 1 5.1821401244249685 1111 2 9.232819600883529 1111 3 7.477558362893934 1111 4 8.087379203831432 1111 5 8.57159827207941 1111 6 7.755519611721667 1111 7 7.619221840327494 1111 8 7.9106205643999274 1111 9 6.428105940807409 1111 10 5.901283546362492 1111 11 7.592471148809416 1111 12 6.88550006544947 1111 13 8.43192350539858 1111 14 6.563912901139684 1111 15 5.853566671325865 1111 16 5.740076821634702 1111 17 7.247948703030314 1111 18 7.033283201609343 1111 19 5.721316119973665 1111 20 5.254665826227228 1111 21 7.90099605213884 1111 22 7.316658935140836 1111 23 7.396173747881132 1111 24 6.909539675887004 1111 25 7.653552743605594 1112 1 -2.038704138951207 1112 2 7.307016883882488 1112 3 5.742718759746083 1112 4 -0.38735957425084067 1112 5 3.575899457653218 1112 6 2.765378632487529 1112 7 5.244377547641708 1112 8 5.423242016105014 1112 9 4.300557836406572 1112 10 3.0092198565754273 1112 11 -0.7010353066326473 1112 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0.9036728028291243 2204 6 3.7701771330972207 2204 7 4.6293151483161346 2204 8 4.69094566070736 2204 9 2.9541324370960496 2204 10 3.00177935798299 2204 11 4.1991935159846605 2204 12 4.293362943903347 2204 13 2.4346302321023003 2204 14 3.9695316176669806 2204 15 4.207562435967123 2204 16 4.164212584396793 2204 17 -1.1457481291237208 2204 18 2.420110224065418 2204 19 3.213203167437147 2204 20 3.608582087547415 2204 21 5.020204054509485 2204 22 4.425021961759995 2204 23 2.8180545073546774 2204 24 4.703076778641432 2204 25 4.810716354212783 2205 1 5.643427294605058 2205 2 10.087367276438462 2205 3 6.978354118862104 2205 4 7.850987328918684 2205 5 7.655515625143767 2205 6 7.318904366712594 2205 7 7.400113226618156 2205 8 7.118742161496115 2205 9 5.974249316487992 2205 10 5.178876733574555 2205 11 7.3547076418989015 2205 12 6.655090320929016 2205 13 9.943112951161527 2205 14 7.560600741430068 2205 15 6.485606319517963 2205 16 5.954557486160741 2205 17 6.665043495714944 2205 18 6.499889372208251 2205 19 5.029587793853324 2205 20 5.4106758778815625 2205 21 -0.6515692138111788 2205 22 3.6871042792703896 2205 23 4.773676190991562 2205 24 6.73672800479947 2205 25 7.223806129058616 2206 1 4.72411988770661 2206 2 8.728788878747105 2206 3 6.5914089491493115 2206 4 2.250152506876635 2206 5 6.209397719413385 2206 6 6.355186488466064 2206 7 6.348927985447343 2206 8 6.18652765002161 2206 9 5.369539271139633 2206 10 4.738021985738911 2206 11 3.6102250254360726 2206 12 5.04447811809818 2206 13 8.288330760698425 2206 14 1.2166093988438362 2206 15 3.562052657294519 2206 16 4.306670307378276 2206 17 5.398058474414956 2206 18 -0.04473066352075605 2206 19 1.5288526007792562 2206 20 3.075991255097449 2206 21 3.8123547933346202 2206 22 -1.8882014785968622 2206 23 -2.2072156600459536 2206 24 1.6318977175932048 2206 25 2.8725934040345678 2207 1 1.427892607264301 2207 2 -13.024474828824696 2207 3 -5.546127208938724 2207 4 -3.832770041686249 2207 5 1.2357078068428287 2207 6 2.0339094624394605 2207 7 3.3585484744888703 2207 8 3.6710297788798236 2207 9 1.2957551037777932 2207 10 0.7518839403882716 2207 11 -1.4115149685691755 2207 12 -2.2561657757442006 2207 13 0.29323670452834705 2207 14 -5.175820966366395 2207 15 -1.1281885410775097 2207 16 -0.21685366620149438 2207 17 0.9478672563613735 2207 18 0.9790551289236937 2207 19 0.3250780755742255 2207 20 0.21165134559649346 2207 21 -3.545652710935734 2207 22 -3.8079819841058935 2207 23 -0.9770299311602919 2207 24 0.5954416977455281 2207 25 1.2051503090105697 2208 1 4.080202777616385 2208 2 5.774259348390496 2208 3 5.3669617137988155 2208 4 7.192509647373047 2208 5 2.797754132805327 2208 6 5.274550602555365 2208 7 5.945632880182444 2208 8 5.501803453967803 2208 9 4.31470567891304 2208 10 3.9149859940675604 2208 11 5.952975463351269 2208 12 5.217565337584581 2208 13 7.881320812587244 2208 14 1.9701110678791025 2208 15 3.746099324181518 2208 16 4.192015847253344 2208 17 4.9592806709314665 2208 18 0.28230109772457723 2208 19 2.3652640097094206 2208 20 3.471635238307943 2208 21 6.020366162604542 2208 22 5.138470035432949 2208 23 1.3511245587463918 2208 24 2.612044460495671 2208 25 3.779319120397723 2209 1 3.15831159272901 2209 2 4.999103534559493 2209 3 5.572806268654059 2209 4 0.9772793940329265 2209 5 5.200205018530145 2209 6 5.702193292911584 2209 7 5.994220460722833 2209 8 5.054936409251429 2209 9 4.656428672645372 2209 10 3.788468674592149 2209 11 -0.08942696646667159 2209 12 3.220607876955004 2209 13 2.4599791457742732 2209 14 4.15385024869337 2209 15 4.604016422445184 2209 16 4.054183371261843 2209 17 1.812503164303891 2209 18 3.6221683706752934 2209 19 4.341877012044431 2209 20 4.631056927593221 2209 21 6.5649666647200995 2209 22 4.9699716418516005 2209 23 1.474592316256178 2209 24 -2.1662539646873533 2209 25 0.40714615387794595 2210 1 -2.0335093392169377 2210 2 -13.024474828824696 2210 3 -7.04112837733272 2210 4 -1.612821451022036 2210 5 -0.4503163062829749 2210 6 -1.0155925490929043 2210 7 -1.1276865811203685 2210 8 -19.72731047008283 2210 9 -11.99875597070259 2210 10 -6.303731613777842 2210 11 -8.954224505821998 2210 12 -4.079795123275101 2210 13 -4.863075465084567 2210 14 -3.5063122602522228 2210 15 -5.4929485150570585 2210 16 -4.669236874169311 2210 17 -4.034960509732464 2210 18 -3.97285756362448 2210 19 -9.157879161205555 2210 20 -6.423178163614875 2210 21 -4.725307497877509 2210 22 -10.269396561873565 2210 23 -7.985480261784213 2210 24 -10.156498783632799 2210 25 -8.048902755023736 2211 1 0.36259018699924894 2211 2 4.640213598475009 2211 3 0.7074342375564662 2211 4 1.663696215378156 2211 5 1.3133947443066596 2211 6 -0.0025894643041191046 2211 7 -19.30120256285553 2211 8 -12.917221449311317 2211 9 -8.983743030849196 2211 10 -6.752936478982058 2211 11 -9.199313910498411 2211 12 -4.483215941760346 2211 13 -6.097828794895008 2211 14 -4.443658980575243 2211 15 -4.788374626913399 2211 16 -11.022144840873462 2211 17 -7.191063016857289 2211 18 -5.268313032914346 2211 19 -8.199781251906476 2211 20 -6.041924465707382 2211 21 -3.9743827387213777 2211 22 -5.5955757144825355 2211 23 -7.0954554660442035 2211 24 -20.31327199520053 2211 25 -20.114351765678226 2212 1 -0.34614842174559257 2212 2 -13.024474828824696 2212 3 -7.033923748666167 2212 4 -18.355591618449736 2212 5 -18.46553700643518 2212 6 -19.38241142276109 2212 7 -8.831089477233558 2212 8 -8.266890437772652 2212 9 -4.246452579609279 2212 10 -4.617041980820478 2212 11 -4.521816463194202 2212 12 -1.8568155544217184 2212 13 -4.972502217616638 2212 14 -2.5651049808724586 2212 15 -2.588885645451004 2212 16 -2.5985651224609754 2212 17 -3.042553647385134 2212 18 -2.200909879556054 2212 19 -2.7333617852640977 2212 20 -2.501831561961989 2212 21 -0.5984125944760912 2212 22 -1.8612334471134204 2212 23 -1.91689859006447 2212 24 -0.7333043053782369 2212 25 -1.3405230096200675 2213 1 -17.388151652763362 2213 2 -13.024474828824696 2213 3 -8.252073773372443 2213 4 -5.357176404503619 2213 5 -18.46553700643518 2213 6 -9.984630123236528 2213 7 -19.30120256285553 2213 8 -10.18689842570882 2213 9 -7.615339056186961 2213 10 -6.8418255768766905 2213 11 -6.349120867568109 2213 12 -5.278867890765035 2213 13 -17.817413364627946 2213 14 -11.526255960546745 2213 15 -6.560344135065407 2213 16 -5.807639110719734 2213 17 -4.4998117882954 2213 18 -4.711129061355848 2213 19 -5.324440899115231 2213 20 -5.514171899247472 2213 21 -3.717123054832115 2213 22 -4.485303761433588 2213 23 -4.326469776144979 2213 24 -3.4004352281640813 2213 25 -3.9210078671045316 2214 1 -17.388151652763362 2214 2 -13.024474828824696 2214 3 -11.443868103360117 2214 4 -18.355591618449736 2214 5 -18.46553700643518 2214 6 -19.38241142276109 2214 7 -15.364165525818493 2214 8 -14.032866025638386 2214 9 -12.765955361874584 2214 10 -12.73320903640595 2214 11 -13.072387874670298 2214 12 -11.30899349973375 2214 13 -17.817413364627946 2214 14 -15.474145847263891 2214 15 -22.088077891008354 2214 16 -16.792372338400664 2214 17 -21.753377556916636 2214 18 -16.339194448454514 2214 19 -13.555012596010224 2214 20 -12.508135038297775 2214 21 -10.081345042271218 2214 22 -10.978782660300759 2214 23 -11.009072347020133 2214 24 -9.918827550756086 2214 25 -9.666203617530078 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 90455.0 3 3.0 4 446.0 5 1612.0 6 2040.0 7 2055.0 8 1838.0 9 1789.0 10 1688.0 11 1546.0 12 1446.0 13 1522.0 14 2166.0 15 4213.0 16 6049.0 17 6703.0 18 6879.0 19 7780.0 20 9314.0 21 10777.0 22 11671.0 23 12409.0 24 13020.0 25 12956.0 26 11199.0 27 9248.0 28 10114.0 29 11485.0 30 11221.0 31 12834.0 32 16245.0 33 19674.0 34 23040.0 35 25198.0 36 23438.0 37 23221.0 38 13384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28439027191704 18.560805988027226 13.242794595023286 24.91200914503245 2 11.804275231939107 20.382134950428338 43.70525037576727 24.108339441865287 3 21.090631618445144 24.910410963733753 27.99030952602804 26.008647891793064 4 11.19320757827634 17.46135985821644 37.46135985821644 33.88407270529078 5 11.582873283185817 38.3379680138685 36.05508023876124 14.024078464184447 6 30.84399459514951 36.592336099573544 19.116745325553456 13.446923979723488 7 26.131862727144302 31.218639305578083 22.732742788675356 19.916755178602262 8 23.345724716713516 41.14016088316769 20.18182562968197 15.332288770436822 9 26.116215373705792 16.63751644905137 19.895915876041375 37.35035230120147 10 14.674611266580376 31.318258840657258 34.0265575248329 19.980572367929465 11 32.5936185751341 23.188949608401117 24.149940819461584 20.067490997003198 12 24.516161539818103 25.905323356763155 31.658982692162347 17.91953241125639 13 29.21922955407113 21.84904766203286 24.164447553710055 24.767275230185952 14 19.26828502946025 25.338473087497317 27.27794982705226 28.11529205599017 15 23.803318925896228 32.842600379417576 22.6129940740451 20.7410866206411 16 19.109732826764844 29.182803126615177 31.844982203964854 19.862481842655125 17 20.59578939651275 29.707115015701174 30.373529160200878 19.323566427585195 18 20.333758631584786 27.80292852818666 33.49259908685491 18.370713753373643 19 22.51234816398651 27.753899705668942 27.45402433233134 22.279727798013205 20 22.54084877727821 29.510529854055317 28.553378755298304 19.395242613368172 21 20.144404870125033 27.329130838660966 27.34602029715512 25.180443994058884 22 20.901694751581285 30.77760610303703 27.32702428661098 20.993674858770703 23 19.711056801064203 29.888558639382612 29.07160755887973 21.328777000673455 24 21.863225860245034 29.509153538690835 28.912727405376952 19.714893195687182 25 21.33962205781216 28.27808092370003 28.678582562115828 21.703714456371976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 858.0 1 858.0 2 1404.0 3 1950.0 4 1950.0 5 1950.0 6 2514.5 7 3079.0 8 3079.0 9 3079.0 10 3576.5 11 4074.0 12 4074.0 13 4074.0 14 4860.5 15 5647.0 16 5647.0 17 5647.0 18 7785.5 19 9924.0 20 9924.0 21 9924.0 22 13702.5 23 17481.0 24 17481.0 25 17481.0 26 22908.5 27 28336.0 28 28336.0 29 28336.0 30 34975.0 31 41614.0 32 41614.0 33 41614.0 34 44585.5 35 47557.0 36 47557.0 37 47557.0 38 48851.5 39 50146.0 40 50146.0 41 50146.0 42 50948.0 43 51750.0 44 51750.0 45 51750.0 46 54040.0 47 56330.0 48 56330.0 49 56330.0 50 49863.0 51 43396.0 52 43396.0 53 43396.0 54 34664.5 55 25933.0 56 25933.0 57 25933.0 58 20801.5 59 15670.0 60 15670.0 61 15670.0 62 12382.5 63 9095.0 64 9095.0 65 9095.0 66 6876.0 67 4657.0 68 4657.0 69 4657.0 70 3323.0 71 1989.0 72 1989.0 73 1989.0 74 1390.5 75 792.0 76 792.0 77 792.0 78 538.5 79 285.0 80 285.0 81 285.0 82 181.0 83 77.0 84 77.0 85 77.0 86 52.0 87 27.0 88 27.0 89 27.0 90 17.5 91 8.0 92 8.0 93 8.0 94 4.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.077698382135505 2 35.79079485972645 3 5.606901240378627 4 13.488463860720076 5 13.063911114914495 6 14.32473293112547 7 9.135728514445727 8 7.151312880635545 9 0.1048307731804373 10 1.379915279620042 11 5.607138951882437 12 5.64517279249212 13 15.73222274518753 14 1.519214220853004 15 2.7645847893162943 16 2.9573688189066223 17 2.7272640832180435 18 1.442195693618397 19 1.3879974707495994 20 0.28620465058786054 21 4.293069758817909 22 1.5351408916083085 23 0.10863415724140554 24 8.769652798577535 25 0.10815873423378451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 420678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.61349945182855 #Duplication Level Percentage of deduplicated Percentage of total 1 96.97912102313465 83.99701045576087 2 1.8222487968791754 3.156626903391794 3 0.45402468561235554 1.1797400057520713 4 0.19870941902220643 0.6884367262221217 5 0.12964560358569055 0.5614529707550595 6 0.069320594177276 0.36024595474643545 7 0.04744766196544697 0.28767256305443506 8 0.03917879464195457 0.27147300065953883 9 0.03408849664176463 0.2657271586675626 >10 0.18772904260096268 3.045076551940106 >50 0.016819755788175076 1.015328005339269 >100 0.01938548111343297 3.323767073563951 >500 0.0017104836276558499 1.0148341633123716 >1k 5.701612092186166E-4 0.8326084668343838 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 1692 0.40220786444739204 No Hit GTACATGGGAAGCAGTGGTATCAAC 1680 0.3993553264016659 No Hit GTCCTACAGTGGACATTTCTAAATT 871 0.20704671981895892 No Hit GAGTACATGGGAAGCAGTGGTATCA 864 0.2053827392922853 No Hit CATGTACTCTGCGTTGATACCACTG 654 0.15546332349207706 No Hit CTGTAGGACGTGGAATATGGCAAGA 615 0.14619257484346698 No Hit GCGTTGATACCACTGCTTCCCATGT 603 0.1433400367977408 No Hit GTCCTAAAGTGTGTATTTCTCATTT 503 0.11956888641668924 No Hit GTATCAACGCAGAGTACATGGGAAG 497 0.11814261739382616 No Hit ACGCAGAGTACATGGGAAGCAGTGG 486 0.11552779085191049 No Hit ACTCTGCGTTGATACCACTGCTTCC 431 0.10245365814233214 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTATAC 30 3.0792324E-4 21.767338 3 GTGCTAA 40 5.583731E-4 19.92786 1 GTGTAGG 50 1.165307E-4 18.599337 1 TAGGACC 185 0.0 16.728973 4 TCTACAC 80 2.4056135E-8 16.325504 3 CTTTAGG 230 0.0 16.173338 1 TGTAGGA 395 0.0 16.165806 2 TTTAGGA 255 0.0 15.983712 2 TCTAGGA 85 1.3175177E-6 15.983712 2 GATATAC 260 0.0 15.8400955 1 CTCCTGA 60 4.6883046E-4 15.499447 1 TTAGGAC 240 0.0 15.418531 3 TTCAGGA 55 0.004052721 14.82126 2 TATAGGA 55 0.004052721 14.82126 2 GTCCTAC 480 0.0 14.66912 1 AGGACCT 340 0.0 14.616627 5 GGACCTG 305 0.0 14.589599 6 TATAGTG 50 7.804271E-4 14.494822 5 GTATAAA 55 0.0047034374 14.4929905 1 CTGTAGG 370 0.0 14.362423 1 >>END_MODULE