FastQCFastQC Report
Thu 26 May 2016
SRR1926503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420678
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC47341.1253262590389799No Hit
CCCATGTACTCTGCGTTGATACCAC46991.1170063564056119No Hit
GAGTACATGGGAAGCAGTGGTATCA23490.5583843224509006No Hit
CTGTAGGACGTGGAATATGGCAAGA18920.4497501652094951No Hit
GTCCTACAGTGGACATTTCTAAATT18510.440003993553264No Hit
CATGTACTCTGCGTTGATACCACTG17590.4181345352026966No Hit
GCGTTGATACCACTGCTTCCCATGT15060.3579935247386362No Hit
ACGCAGAGTACATGGGAAGCAGTGG14580.34658337255573146No Hit
GTATCAACGCAGAGTACATGGGAAG13930.331132124808048No Hit
GTATCAACGCAGAGTACTTTTTTTT13470.3201973956327642No Hit
GTCCTAAAGTGTGTATTTCTCATTT11480.27289280637447166No Hit
CTTTAGGACGTGAAATATGGCGAGG11360.2700402683287455No Hit
ACTCTGCGTTGATACCACTGCTTCC10470.24888394448960963No Hit
GATATACACTGTTCTACAAATCCCG10370.24650682945150446No Hit
GTCCTACAGTGTGCATTTCTCATTT10300.24484284892483088No Hit
GCTTCCCATGTACTCTGCGTTGATA9700.23058015869619997No Hit
TATCAACGCAGAGTACATGGGAAGC9220.21917000651329518No Hit
GGTATCAACGCAGAGTACATGGGAA8750.20799756583420098No Hit
GGTATCAACGCAGAGTACTTTTTTT8540.20300562425418014No Hit
CTGTAGGACCTGGAATATGGCGAGA8450.20086622071988552No Hit
GTGTATATCAATGAGTTACAATGAA7370.17519337830834986No Hit
CTGAAGGACCTGGAATATGGCGAGA7340.1744802437969183No Hit
TATCAACGCAGAGTACTTTTTTTTT7080.16829974469784492No Hit
GTGTATATCAATGAGTTACAATGAG6930.16473407214068717No Hit
CAGTGGTATCAACGCAGAGTACATG6770.16093068807971894No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6530.15522561198826657No Hit
GATATACACTGTTCTACAATGCCGG6300.1497582474006247No Hit
CATGGGAAGCAGTGGTATCAACGCA6010.14286461379011975No Hit
GTGGTATCAACGCAGAGTACATGGG5860.13929894123296202No Hit
ACATGGGAAGCAGTGGTATCAACGC5670.13478242266056223No Hit
GCAGTGGTATCAACGCAGAGTACAT5550.13192988461483607No Hit
GTTCTACAGTGTGGTTTTTATCATT5540.13169217311102555No Hit
TTGTAGAACAGTGTATATCAATGAG5250.1247985395005206No Hit
ATACCACTGCTTCCCATGTACTCTG4890.11624092536334203No Hit
CTGTAGAACATATTAGATGAGTGAG4720.11219982979856326No Hit
ACCTGGAATATGGCGAGAAAACTGA4620.10982271476045811No Hit
GTTCTACAAATCCCGTTTCCAACGA4340.10316679265376368No Hit
CCATGTACTCTGCGTTGATACCACT4260.10126510062327956No Hit
GAATATGGCAAGAAAACTGAAAATC4260.10126510062327956No Hit
GGGAAGCAGTGGTATCAACGCAGAG4220.1003142546080375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG453.4314013E-516.9441591
TTAGTCT350.002114781916.3448734
TAACCTG456.5647194E-414.8314595
GTGTAGG456.582611E-414.82613851
TAGGACC2600.014.3017654
TAAGGCT400.00514528114.30176454
GTGTAAG400.00515754914.2966341
ACTGTTC3350.013.8977898
CTATAGG706.9922135E-613.6158421
CCAACGA1051.4133548E-913.53492119
TATAGGA801.8998307E-613.1162262
CTTCTAC801.9177915E-613.10524751
TGTAGGA9350.013.0588392
TCCAACG1600.013.02736118
CTGTAGG9100.012.8826811
TGCACTG603.9579658E-412.712685
GTAGACC701.04986284E-412.2601213
CACTGTT3650.012.2334147
TGTACTA550.002979954212.1348315
AAAAAGT1103.6154233E-812.1304791
Produced by FastQC (version 0.11.5)