FastQCFastQC Report
Thu 26 May 2016
SRR1926502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987222
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47980.1606174566202311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA508.849051E-515.1734789
GTCCTAA2900.010.5266881
GTATTAT2200.010.4070661
GGTATCA16150.010.3963261
GTATCAA30800.09.9424641
GTATAAA4100.09.539811
ATTAGAC2053.965397E-109.3005523
GTCTAGA3300.09.2507261
AACCGTG1451.6637732E-69.1899557
GAACCGT1054.4080272E-49.0652266
GTCTAAT1909.857104E-99.0377151
GTCTTAT2750.09.0194571
AGAACCG1951.596527E-88.7862955
AGGACCG2201.54796E-98.653175
GGACCGT1651.0217154E-68.653176
ACCGTAT1107.0691016E-48.6459058
GTCTAAG3100.08.6166031
GTGTACG1553.978761E-68.6166031
ATAGACG1000.00281603038.579763
TAGGACC3350.08.5278874
Produced by FastQC (version 0.11.5)