FastQCFastQC Report
Thu 26 May 2016
SRR1926502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987222
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88840.29740005931932745No Hit
GGTATCAACGCAGAGTACTTTTTTT58460.19570021913336202No Hit
TATCAACGCAGAGTACTTTTTTTTT52510.1757820476683688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21900.011.4056071
GTCCTAA3350.09.9605761
GTATCAA42500.09.690721
TAGACAG4700.09.5239225
TACACCG1304.1079165E-69.5239225
GTACCGT800.00442474489.5239216
TTAAACG800.00442474489.5239214
GTCCTAG3850.09.1622511
CTTACAC3350.09.0979363
CTGCGTT4900.08.90625211
GTCTAGA3250.08.8003331
GTCTAAG3150.08.7770521
GATTAGT1201.6981424E-48.739221
CCCTTAA3400.08.6924861
TGCGTTG5150.08.65783412
ACCGTCC1000.00294022388.5389578
GTCTTAT2803.6379788E-128.5122271
TATACTG3600.08.4657085
TATACAG6000.08.4127985
TTAATCC5000.08.3810514
Produced by FastQC (version 0.11.5)