FastQCFastQC Report
Thu 26 May 2016
SRR1926501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13773
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA2661.9313148914542948No Hit
GTACATGGGAAGCAGTGGTATCAAC1481.0745661802076527No Hit
CCCATGTACTCTGCGTTGATACCAC1391.0092209395193494No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA730.5300225078051259No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA630.4574166848181224No Hit
GAGTACATGGGAAGCAGTGGTATCA600.43563493792202135No Hit
ACGCAGAGTACATGGGAAGCAGTGG580.42111377332462063No Hit
GCGTTGATACCACTGCTTCCCATGT500.36302911493501777No Hit
CATGTACTCTGCGTTGATACCACTG500.36302911493501777No Hit
GCTTACTCTGCGTTGATACCACTGC400.29042329194801425No Hit
GGTATCAACGCAGAGTACATGGGAA330.23959921585711175No Hit
GTATCAACGCAGAGTACATGGGAAG330.23959921585711175No Hit
CNCATGTACTCTGCGTTGATACCAC330.23959921585711175No Hit
GAGTAAGCAGTGGTATCAACGCAGA320.23233863355841136No Hit
ACTCTGCGTTGATACCACTGCTTCC320.23233863355841136No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA310.225078051259711No Hit
AAACAAAAAAAAAAAAAAAAAAAAA310.225078051259711No Hit
TATCAACGCAGAGTACATGGGAAGC300.21781746896101067No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA260.18877513976620924No Hit
CTGCTTACTCTGCGTTGATACCACT250.18151455746750889No Hit
CATGGGAAGCAGTGGTATCAACGCA240.17425397516880856No Hit
CCACTGCTTCCCATGTACTCTGCGT230.1669933928701082No Hit
GNACATGGGAAGCAGTGGTATCAAC230.1669933928701082No Hit
GTACTCTGCGTTGATACCACTGCTT200.14521164597400713No Hit
ACATGGGAAGCAGTGGTATCAACGC190.13795106367530677No Hit
GTATCAACGCAGAGTACTTTTTTTT180.1306904813766064No Hit
CTGCTTCCCATGTACTCTGCGTTGA180.1306904813766064No Hit
ANAAAAANAAAAAAAAAAAAAAAAA180.1306904813766064No Hit
GGGAAGCAGTGGTATCAACGCAGAG180.1306904813766064No Hit
GTGGTATCAACGCAGAGTACATGGG170.12342989907790605No Hit
CCCNTGTACTCTGCGTTGATACCAC170.12342989907790605No Hit
GNGTACATGGGAAGCAGTGGTATCA160.11616931677920568No Hit
GCAGAGTACATGGGAAGCAGTGGTA160.11616931677920568No Hit
ANAANANAAAAANAACAAAAAAAAA150.10890873448050534No Hit
CAGTGGTATCAACGCAGAGTACATG140.10164815218180498No Hit
GCAGTGGTATCAACGCAGAGTACAT140.10164815218180498No Hit
AAAAANAAAAAANCAAAAAAAAAAA140.10164815218180498No Hit
GNGTAAGCAGTGGTATCAACGCAGA140.10164815218180498No Hit
ACGCAGAGTAAGCAGTGGTATCAAC140.10164815218180498No Hit
GATACCACTGCTTCCCATGTACTCT140.10164815218180498No Hit
ACGCAGAGTACTTTTTTTTTTTTTT140.10164815218180498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTC450.0050881714.1192985
CTGCGTT800.0042810569.4018711
TCTGCGT800.0042810569.4018710
CTCTGCG850.00705809158.8488199
Produced by FastQC (version 0.11.5)