##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926501_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13773 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.958542075074423 25.0 2.0 30.0 2.0 31.0 2 14.096275321280766 2.0 2.0 30.0 2.0 31.0 3 17.43933783489436 16.0 2.0 30.0 2.0 31.0 4 19.120017425397517 23.0 2.0 33.0 2.0 35.0 5 19.574675088942133 25.0 2.0 35.0 2.0 35.0 6 20.129528788208816 28.0 2.0 35.0 2.0 37.0 7 20.256298555144124 26.0 2.0 35.0 2.0 37.0 8 20.112248602337907 26.0 2.0 35.0 2.0 37.0 9 22.357728889856965 28.0 2.0 37.0 2.0 39.0 10 23.124664198068686 30.0 2.0 37.0 2.0 39.0 11 20.641545051913162 26.0 2.0 35.0 2.0 39.0 12 21.785086763958468 28.0 2.0 35.0 2.0 39.0 13 18.67530675960212 18.0 2.0 35.0 2.0 39.0 14 21.714296086546142 27.0 2.0 36.0 2.0 40.0 15 22.643650620779788 29.0 2.0 37.0 2.0 40.0 16 22.88078123865534 30.0 2.0 37.0 2.0 40.0 17 22.335148478908007 30.0 2.0 37.0 2.0 40.0 18 22.352428664778916 30.0 2.0 36.0 2.0 40.0 19 22.5592100486459 30.0 2.0 37.0 2.0 40.0 20 22.713933057431205 30.0 2.0 37.0 2.0 40.0 21 20.980469033616497 28.0 2.0 35.0 2.0 40.0 22 21.728236404559645 30.0 2.0 36.0 2.0 40.0 23 21.65229071371524 30.0 2.0 36.0 2.0 40.0 24 20.82168009874392 29.0 2.0 37.0 2.0 40.0 25 20.630073331881217 28.0 2.0 36.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.6895803584922575 1101 2 9.8934736055537 1101 3 8.002874835754124 1101 4 9.550815181851796 1101 5 10.24414733372399 1101 6 9.075747773626492 1101 7 7.189182517224278 1101 8 7.188413700736927 1101 9 8.894794916266886 1101 10 5.961457024393535 1101 11 9.740523364803686 1101 12 5.491679152506961 1101 13 11.050510013632437 1101 14 8.34226958104189 1101 15 5.907656154951919 1101 16 5.864205585032813 1101 17 5.706907744895158 1101 18 6.68718899987207 1101 19 7.6336605638619375 1101 20 0.9710729612154054 1101 21 0.9498817574492513 1101 22 4.138969939353796 1101 23 7.299387260188745 1101 24 8.48640645503746 1101 25 10.040838273007608 1102 1 8.621257377125797 1102 2 13.698856628328029 1102 3 9.766849991033627 1102 4 9.875866941686162 1102 5 10.11992373123952 1102 6 7.910116303647197 1102 7 8.04011419424291 1102 8 6.196695274235893 1102 9 5.520053715438728 1102 10 6.125018100998091 1102 11 7.191869120497266 1102 12 6.686296129732632 1102 13 10.563967570568256 1102 14 8.18284929118682 1102 15 6.319664436525418 1102 16 5.441845336585608 1102 17 6.91601747574402 1102 18 5.58366933113501 1102 19 6.265130543158005 1102 20 4.608754120635695 1102 21 0.4840432481324797 1102 22 3.3646428172005862 1102 23 4.386343781927874 1102 24 4.927400243857338 1102 25 5.587422123939284 1103 1 6.634635303547203 1103 2 9.578455290535386 1103 3 7.480896813776102 1103 4 10.827371958408573 1103 5 9.815575905152564 1103 6 8.747908945787664 1103 7 9.244127572169333 1103 8 9.129805642128868 1103 9 9.354501875973842 1103 10 8.342409405345915 1103 11 8.084845709126029 1103 12 8.460543621371428 1103 13 11.109118072240495 1103 14 9.316628555400865 1103 15 8.414982162277926 1103 16 7.891678112505339 1103 17 9.18859272658014 1103 18 4.381328194011264 1103 19 6.62633455653593 1103 20 7.2659447560871975 1103 21 9.93889274646024 1103 22 6.695746496130351 1103 23 7.568618029419515 1103 24 8.096296564927574 1103 25 4.057321789491123 1104 1 8.490822594517102 1104 2 12.090160976154115 1104 3 8.5494586866858 1104 4 9.484562593860076 1104 5 10.511228079065607 1104 6 10.388377173212412 1104 7 7.953157672503782 1104 8 9.066260491627197 1104 9 7.128749367612642 1104 10 7.038061579258958 1104 11 9.409260424845094 1104 12 7.8167309123413276 1104 13 11.52048930969869 1104 14 7.487197117273777 1104 15 6.971838349568895 1104 16 6.789671423542131 1104 17 7.002973997483149 1104 18 3.4532345485263143 1104 19 4.178174021418876 1104 20 4.347884555418304 1104 21 5.049260639436827 1104 22 5.277686295461457 1104 23 5.560256825406135 1104 24 5.796965461248643 1104 25 6.022204732634936 1105 1 3.362199406111305 1105 2 8.280378367458463 1105 3 4.246922454801741 1105 4 4.622243753280365 1105 5 -1.8094240948474365 1105 6 2.7959858688645873 1105 7 5.849896802938563 1105 8 2.616985129308354 1105 9 4.126937773409743 1105 10 3.259076072012583 1105 11 6.097666221946543 1105 12 3.8547743906021985 1105 13 5.2469385850610095 1105 14 3.508936247708558 1105 15 5.770751393047156 1105 16 4.215396061223288 1105 17 5.486669649657063 1105 18 3.0800461427292127 1105 19 2.3538986591000324 1105 20 3.1377396278820697 1105 21 2.5094055669730615 1105 22 3.067541367925223 1105 23 1.8827205935220768 1105 24 4.016168359799366 1105 25 3.368219225388561 1106 1 5.722047890959789 1106 2 11.337196549276646 1106 3 7.834043666922955 1106 4 10.10709223812885 1106 5 9.224666814243474 1106 6 7.852804050682771 1106 7 8.156714984756746 1106 8 8.101833614156838 1106 9 7.857998379470349 1106 10 7.342409405345915 1106 11 8.158272282552602 1106 12 5.775228936056745 1106 13 1.8643628274852517 1106 14 5.918027156799468 1106 15 7.054842302138066 1106 16 7.6434263642535925 1106 17 7.65712419511161 1106 18 7.314894627577697 1106 19 6.441019871221247 1106 20 7.531679021821464 1106 21 5.32001162757912 1106 22 7.779662580046438 1106 23 7.890296351097835 1106 24 9.648744117375124 1106 25 7.1901889223582565 1107 1 0.3654045343164327 1107 2 -12.344621632541537 1107 3 -5.980641647762361 1107 4 -0.7880126569760435 1107 5 0.8155759051525635 1107 6 1.5940627919415107 1107 7 2.244127572169333 1107 8 3.822113334436562 1107 9 1.6237326452046155 1107 10 0.7654863284228384 1107 11 2.9309995552798753 1107 12 1.7297743906021985 1107 13 0.6475796107020351 1107 14 3.008936247708558 1107 15 1.453443700739463 1107 16 1.891678112505339 1107 17 0.9962850342724465 1107 18 -5.234056421373349 1107 19 -1.6813577511563764 1107 20 -0.9648244746820325 1107 21 -2.484184176616683 1107 22 -0.7657919654081091 1107 23 -1.508305047503562 1107 24 0.21168118031218697 1107 25 0.0957833279526632 1108 1 6.012561724951887 1108 2 10.394508802241072 1108 3 0.027719556251017963 1108 4 7.09325824603399 1108 5 5.9025324268916926 1108 6 6.344898912342849 1108 7 7.300983759460301 1108 8 7.2401735351054555 1108 9 6.607010237177857 1108 10 3.1250181009980906 1108 11 5.757086511801614 1108 12 0.8167309123413276 1108 13 3.085706701003037 1108 14 5.095892769447687 1108 15 4.537055740873242 1108 16 5.137497510498651 1108 17 2.0029739974831493 1108 18 5.23584324417849 1108 19 5.265130543158005 1108 20 6.130493251070476 1108 21 6.527521509002046 1108 22 4.4081210780701525 1108 23 5.516778564536569 1108 24 6.927400243857338 1108 25 7.717856906547979 1109 1 4.4538660727779735 1109 2 -12.344621632541537 1109 3 -3.811410878531591 1109 4 3.338910419947034 1109 5 2.865575905152564 1109 6 5.370985868864587 1109 7 6.824896802938564 1109 8 5.8336517959750225 1109 9 6.585271106743075 1109 10 6.042409405345914 1109 11 4.330999555279874 1109 12 5.9797743906021985 1109 13 2.7552719183943424 1109 14 5.458936247708557 1109 15 6.845751393047156 1109 16 6.857062727889957 1109 17 6.011669649657062 1109 18 6.746712809395881 1109 19 6.3955653257667 1109 20 5.554406294548738 1109 21 3.4927389003063922 1109 22 6.084208034591892 1109 23 6.649387260188746 1109 24 7.657835026466035 1109 25 8.026552558721892 1110 1 -0.7211339272220272 1110 2 5.113711700791795 1110 3 2.246922454801741 1110 4 4.622243753280365 1110 5 3.6905759051525635 1110 6 1.1293192021979195 1110 7 3.183230136271895 1110 8 3.7836517959750218 1110 9 1.9602711067430754 1110 10 2.384076072012583 1110 11 1.3893328886132075 1110 12 2.3131077239355307 1110 13 4.788605251727674 1110 14 3.96726958104189 1110 15 4.645751393047156 1110 16 4.215396061223288 1110 17 3.4450029829903954 1110 18 4.580046142729213 1110 19 3.8955653257667002 1110 20 4.179406294548738 1110 21 -0.40726109969360635 1110 22 2.692541367925223 1110 23 3.4660539268554125 1110 24 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5.371922454801741 2204 4 9.622243753280365 2204 5 4.3155759051525635 2204 6 6.462652535531255 2204 7 7.849896802938563 2204 8 7.533651795975022 2204 9 7.335271106743075 2204 10 7.425742738679247 2204 11 7.805999555279875 2204 12 8.021441057268866 2204 13 7.455271918394342 2204 14 7.59226958104189 2204 15 7.8540847263804885 2204 16 8.007062727889956 2204 17 4.361669649657063 2204 18 6.496712809395881 2204 19 6.062231992433368 2204 20 6.13773962788207 2204 21 7.3844055669730615 2204 22 7.150874701258559 2204 23 7.8410539268554125 2204 24 8.641168359799366 2204 25 10.118219225388561 2205 1 4.267502436414336 2205 2 7.837196549276646 2205 3 6.015861848741135 2205 4 5.379819510856123 2205 5 6.088303177879837 2205 6 6.398258596137314 2205 7 5.20216953021129 2205 8 6.4200154323386585 2205 9 3.312543834015802 2205 10 1.7060457689822783 2205 11 5.203726828007149 2205 12 4.7297743906021985 2205 13 5.773453736576158 2205 14 4.054390793163105 2205 15 3.6457513930471563 2205 16 3.5525172733445025 2205 17 5.475306013293427 2205 18 5.542167354850427 2205 19 0.8046562348576103 2205 20 2.2134972036396476 2205 21 -1.3163520087845164 2205 22 1.4160262164100743 2205 23 1.8448418056432914 2205 24 3.739653208284217 2205 25 3.7810980132673464 2206 1 4.6311388000506994 2206 2 7.791742003822099 2206 3 7.424952757832045 2206 4 6.10709223812885 2206 5 7.224666814243474 2206 6 6.443713141591861 2206 7 6.2930786211203795 2206 8 4.465469977793205 2206 9 3.3579983794703487 2206 10 2.7060457689822783 2206 11 2.8400904643707854 2206 12 2.866138026965835 2206 13 4.682544645667068 2206 14 -2.8092455704732586 2206 15 -1.3997031524073904 2206 16 -0.22020999938277086 2206 17 1.6116696496570633 2206 18 -4.366923554240483 2206 19 -1.2407983105969365 2206 20 0.8044062945487376 2206 21 1.1836479912154836 2206 22 0.006935307319164252 2206 23 -2.836976376174892 2206 24 0.6032895719205804 2206 25 3.0083707405400766 2207 1 -2.732497563585664 2207 2 -12.344621632541537 2207 3 -7.711410878531591 2207 4 -7.07472594368933 2207 5 -4.502605913029255 2207 6 -4.374468676589959 2207 7 -3.570557742515984 2207 8 -3.1254391131158883 2207 9 -4.414728893256925 2207 10 -4.9757724128359015 2207 11 -4.569000444720125 2207 12 -4.542952882125075 2207 13 -4.953818990696567 2207 14 -5.263791025018715 2207 15 -4.17243042513466 2207 16 -3.083846363019134 2207 17 -2.5246939867065734 2207 18 -2.9123780996950295 2207 19 -5.69534376514239 2207 20 -6.695593705451262 2207 21 -8.225442917875425 2207 22 -9.129428329044472 2207 23 -9.473340012538529 2207 24 -8.169437700806693 2207 25 -7.764356532187199 2208 1 5.490822594517102 2208 2 7.437987063110638 2208 3 7.114676077990147 2208 4 8.875866941686162 2208 5 5.032967209500388 2208 6 6.692724999299369 2208 7 8.257505498590739 2208 8 7.5445213611924125 2208 9 6.693966758916989 2208 10 5.864148535780696 2208 11 8.409260424845094 2208 12 7.294991781906546 2208 13 10.085706701003037 2208 14 2.1828492911868196 2208 15 4.797925306090633 2208 16 6.702714901802999 2208 17 8.176887040961411 2208 18 3.8010606354828376 2208 19 5.308608804027571 2208 20 5.000058468461781 2208 21 8.440564987262917 2208 22 6.538555860678848 2208 23 4.038517694971354 2208 24 5.362182852552991 2208 25 6.891769950026241 2209 1 2.596173765085666 2209 2 5.001532213612309 2209 3 5.865512198391485 2209 4 4.59660272763934 2209 5 6.507883597460257 2209 6 5.709447407326127 2209 7 6.128742956784716 2209 8 4.283651795975022 2209 9 4.200655722127692 2209 10 3.5347170976536084 2209 11 -1.7997696754893546 2209 12 2.6143897752175818 2209 13 3.7245026876251117 2209 14 4.739705478477788 2209 15 5.222674469970233 2209 16 4.199370420197649 2209 17 0.9962850342724465 2209 18 3.5351743478574207 2209 19 2.472488402689777 2209 20 2.8813293714718142 2209 21 4.5542773618448535 2209 22 4.118823419207274 2209 23 -1.3929204321189488 2209 24 -3.557549588918583 2209 25 -1.1734474412781069 2210 1 -2.2461339272220258 2210 2 -12.344621632541537 2210 3 -7.261410878531592 2210 4 -5.111089580052967 2210 5 -2.584424094847435 2210 6 -3.179014131135414 2210 7 -2.025103197061437 2210 8 -18.71634820402498 2210 9 -12.414728893256925 2210 10 -8.107590594654084 2210 11 -10.869000444720125 2210 12 -4.620225609397803 2210 13 -3.4447280816056587 2210 14 -3.741063752291442 2210 15 -5.404248606952844 2210 16 -6.042937272110045 2210 17 -3.6883303503429374 2210 18 -3.80328719060412 2210 19 -6.004434674233298 2210 20 -7.495593705451263 2210 21 -7.507261099693606 2210 22 -8.81579196540811 2210 23 -9.650612739811255 2210 24 -11.442164973533966 2210 25 -8.223447441278108 2211 1 2.603866072777972 2211 2 6.605378367458462 2211 3 2.8385891214684094 2211 4 4.788910419947033 2211 5 2.665575905152565 2211 6 3.6209858688645866 2211 7 -18.525103197061437 2211 8 -11.716348204024978 2211 9 -5.114728893256924 2211 10 -2.5575905946540836 2211 11 -6.419000444720124 2211 12 -2.1702256093978 2211 13 -6.844728081605659 2211 14 -2.1910637522914413 2211 15 -3.304248606952843 2211 16 -9.592937272110044 2211 17 -6.588330350342936 2211 18 -5.903287190604118 2211 19 -7.054434674233299 2211 20 -6.095593705451261 2211 21 -3.457261099693607 2211 22 -4.265791965408109 2211 23 -7.000612739811256 2211 24 -18.942164973533966 2211 25 -18.673447441278107 2212 1 -1.641588472676574 2212 2 -12.344621632541537 2212 3 -7.256865423986136 2212 4 -17.211089580052967 2212 5 -18.184424094847436 2212 6 -18.829014131135413 2212 7 -9.206921378879619 2212 8 -9.170893658570433 2212 9 -4.369274347802378 2212 10 -5.066681503744995 2212 11 -5.569000444720125 2212 12 -3.1793165184887116 2212 13 -4.63563717251475 2212 14 -3.036518297745989 2212 15 -2.7633395160437537 2212 16 -3.083846363019134 2212 17 -2.7519667139793 2212 18 -2.685105372422303 2212 19 -2.6953437651423897 2212 20 -4.559230069087626 2212 21 -1.225442917875423 2212 22 -1.7657919654081091 2212 23 -1.7460672852658021 2212 24 -0.9876195189885131 2212 25 -1.082538350369017 2213 1 -16.596133927222027 2213 2 -12.344621632541537 2213 3 -8.266966434087147 2213 4 -6.766645135608522 2213 5 -18.184424094847436 2213 6 -11.051236353357634 2213 7 -18.525103197061437 2213 8 -10.383014870691644 2213 9 -10.025840004368035 2213 10 -6.713146150209642 2213 11 -6.069000444720125 2213 12 -5.436892276064469 2213 13 -17.04472808160566 2213 14 -11.435508196735887 2213 15 -7.687581940286178 2213 16 -6.270715049887823 2213 17 -5.443885905898494 2213 18 -4.947731635048562 2213 19 -5.326656896455521 2213 20 -5.917815927673484 2213 21 -5.851705544138051 2213 22 -6.210236409852554 2213 23 -5.922834962033477 2213 24 -4.942164973533966 2213 25 -6.840114107944773 2214 1 -16.596133927222027 2214 2 -12.344621632541537 2214 3 -12.616172783293496 2214 4 -17.211089580052967 2214 5 -18.184424094847436 2214 6 -18.829014131135413 2214 7 -16.144150816109054 2214 8 -15.383014870691646 2214 9 -15.771871750399782 2214 10 -15.133781070844561 2214 11 -14.78328615900584 2214 12 -13.936892276064468 2214 13 -17.04472808160566 2214 14 -16.276778038005727 2214 15 -20.854248606952844 2214 16 -17.373889653062427 2214 17 -20.388330350342937 2214 18 -17.31281100012793 2214 19 -15.556815626614252 2214 20 -13.981307991165549 2214 21 -12.383451575884083 2214 22 -12.956268155884299 2214 23 -12.986327025525542 2214 24 -12.608831640200632 2214 25 -11.959161726992393 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 3859.0 3 0.0 4 12.0 5 54.0 6 79.0 7 73.0 8 53.0 9 67.0 10 54.0 11 69.0 12 45.0 13 49.0 14 73.0 15 149.0 16 211.0 17 228.0 18 255.0 19 347.0 20 307.0 21 322.0 22 362.0 23 357.0 24 379.0 25 403.0 26 310.0 27 241.0 28 276.0 29 302.0 30 291.0 31 324.0 32 417.0 33 549.0 34 594.0 35 688.0 36 758.0 37 704.0 38 512.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.134612000703854 21.863452401900403 10.11789547774063 22.88404011965511 2 13.165537270087125 27.17812197483059 30.977734753146173 28.678606001936107 3 21.92948469585432 32.227818674932195 24.16117783804727 21.681518791166216 4 12.280850343016853 23.757093249767085 29.60955365461167 34.35250275260439 5 11.411702502643637 44.9947127247092 29.740923510750793 13.85266126189637 6 30.88169342541932 35.86868777468831 16.127006906449008 17.122611893443356 7 23.453834490463652 38.504508818131036 19.35998723166547 18.681669459739844 8 26.269315673289185 36.489248630528984 17.610988471915622 19.63044722426621 9 25.009079683300644 23.578121595118763 17.752596789423986 33.66020193215661 10 18.00678666273237 34.86279138388905 25.64915904396577 21.481262909412806 11 27.637490317583268 30.890782339271883 18.009295120061967 23.46243222308288 12 24.532095901005412 35.06573859242073 22.645011600928076 17.757153905645783 13 27.882427882427884 26.744926744926744 19.8016198016198 25.57102557102557 14 20.045776727702304 31.71145894861193 23.1320141760189 25.110750147666867 15 28.974205158968207 30.90881823635273 18.903719256148772 21.21325734853029 16 18.351442487823157 33.34582240539528 25.919820157362306 22.38291494941926 17 22.978851057447127 33.96580170991451 23.068846557672114 19.98650067496625 18 23.160380839914385 31.43405417373976 24.496272787659606 20.90929219868625 19 20.56067871634083 35.44079675396532 24.559203246034674 19.439321283659165 20 23.081399578089766 31.483232705317526 22.230304793773186 23.205062922819526 21 20.69387131285343 32.943603851444294 24.09445208619899 22.268072749503286 22 21.995565410199557 31.596452328159646 20.051736881005176 26.356245380635624 23 19.56995496149935 34.17114630248438 24.44428301612669 21.81461571988958 24 23.172976867045545 35.2997678700072 20.739614183943008 20.787641079004242 25 21.678169269887395 31.020704685797313 20.90810025426807 26.39302579004722 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 523.0 1 523.0 2 472.0 3 421.0 4 421.0 5 421.0 6 333.5 7 246.0 8 246.0 9 246.0 10 234.0 11 222.0 12 222.0 13 222.0 14 208.0 15 194.0 16 194.0 17 194.0 18 225.0 19 256.0 20 256.0 21 256.0 22 294.5 23 333.0 24 333.0 25 333.0 26 428.0 27 523.0 28 523.0 29 523.0 30 630.0 31 737.0 32 737.0 33 737.0 34 809.0 35 881.0 36 881.0 37 881.0 38 973.0 39 1065.0 40 1065.0 41 1065.0 42 1261.5 43 1458.0 44 1458.0 45 1458.0 46 1847.0 47 2236.0 48 2236.0 49 2236.0 50 2009.0 51 1782.0 52 1782.0 53 1782.0 54 1398.0 55 1014.0 56 1014.0 57 1014.0 58 858.0 59 702.0 60 702.0 61 702.0 62 634.0 63 566.0 64 566.0 65 566.0 66 446.0 67 326.0 68 326.0 69 326.0 70 241.5 71 157.0 72 157.0 73 157.0 74 121.0 75 85.0 76 85.0 77 85.0 78 58.5 79 32.0 80 32.0 81 32.0 82 22.5 83 13.0 84 13.0 85 13.0 86 7.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 17.476221592971754 2 39.99854788354026 3 6.302185435271909 4 14.2743047992449 5 17.60691207434836 6 19.051767951789735 7 9.017643214985842 8 11.195817904595948 9 0.04356349379220214 10 1.5755463588179772 11 6.265882523778407 12 6.1206708778043994 13 20.21346111958179 14 1.6626733464023813 15 3.1728744645320557 16 3.1075292238437524 17 3.187395629129456 18 1.6263704349088797 19 1.5828069411166774 20 0.18877513976620924 21 4.988020039207145 22 1.7643214985841864 23 0.05082407609090249 24 9.293545342336456 25 0.05808465838960284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 13773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.67959050315835 #Duplication Level Percentage of deduplicated Percentage of total 1 95.26637868970482 76.86052421404197 2 2.546796256299496 4.109489581064402 3 0.7289416846652268 1.7643214985841864 4 0.3509719222462203 1.1326508385972556 5 0.206983441324694 0.834966964350541 6 0.10799136069114472 0.5227619255064256 7 0.16198704103671707 0.9148333696362448 8 0.017998560115190784 0.11616931677920568 9 0.08999280057595392 0.653452406883032 >10 0.45896328293736505 7.231539969505555 >50 0.03599712023038157 1.8441879038698903 >100 0.02699784017278618 4.0151020111812965 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 266 1.9313148914542948 No Hit GTACATGGGAAGCAGTGGTATCAAC 148 1.0745661802076527 No Hit CCCATGTACTCTGCGTTGATACCAC 139 1.0092209395193494 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 73 0.5300225078051259 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 63 0.4574166848181224 No Hit GAGTACATGGGAAGCAGTGGTATCA 60 0.43563493792202135 No Hit ACGCAGAGTACATGGGAAGCAGTGG 58 0.42111377332462063 No Hit GCGTTGATACCACTGCTTCCCATGT 50 0.36302911493501777 No Hit CATGTACTCTGCGTTGATACCACTG 50 0.36302911493501777 No Hit GCTTACTCTGCGTTGATACCACTGC 40 0.29042329194801425 No Hit GGTATCAACGCAGAGTACATGGGAA 33 0.23959921585711175 No Hit GTATCAACGCAGAGTACATGGGAAG 33 0.23959921585711175 No Hit CNCATGTACTCTGCGTTGATACCAC 33 0.23959921585711175 No Hit GAGTAAGCAGTGGTATCAACGCAGA 32 0.23233863355841136 No Hit ACTCTGCGTTGATACCACTGCTTCC 32 0.23233863355841136 No Hit ACAAAAAAAAAAAAAAAAAAAAAAA 31 0.225078051259711 No Hit AAACAAAAAAAAAAAAAAAAAAAAA 31 0.225078051259711 No Hit TATCAACGCAGAGTACATGGGAAGC 30 0.21781746896101067 No Hit GNAAAAAAAAAAAAAAAAAAAAAAA 26 0.18877513976620924 No Hit CTGCTTACTCTGCGTTGATACCACT 25 0.18151455746750889 No Hit CATGGGAAGCAGTGGTATCAACGCA 24 0.17425397516880856 No Hit CCACTGCTTCCCATGTACTCTGCGT 23 0.1669933928701082 No Hit GNACATGGGAAGCAGTGGTATCAAC 23 0.1669933928701082 No Hit GTACTCTGCGTTGATACCACTGCTT 20 0.14521164597400713 No Hit ACATGGGAAGCAGTGGTATCAACGC 19 0.13795106367530677 No Hit GTATCAACGCAGAGTACTTTTTTTT 18 0.1306904813766064 No Hit CTGCTTCCCATGTACTCTGCGTTGA 18 0.1306904813766064 No Hit ANAAAAANAAAAAAAAAAAAAAAAA 18 0.1306904813766064 No Hit GGGAAGCAGTGGTATCAACGCAGAG 18 0.1306904813766064 No Hit GTGGTATCAACGCAGAGTACATGGG 17 0.12342989907790605 No Hit CCCNTGTACTCTGCGTTGATACCAC 17 0.12342989907790605 No Hit GNGTACATGGGAAGCAGTGGTATCA 16 0.11616931677920568 No Hit GCAGAGTACATGGGAAGCAGTGGTA 16 0.11616931677920568 No Hit ANAANANAAAAANAACAAAAAAAAA 15 0.10890873448050534 No Hit CAGTGGTATCAACGCAGAGTACATG 14 0.10164815218180498 No Hit GCAGTGGTATCAACGCAGAGTACAT 14 0.10164815218180498 No Hit AAAAANAAAAAANCAAAAAAAAAAA 14 0.10164815218180498 No Hit GNGTAAGCAGTGGTATCAACGCAGA 14 0.10164815218180498 No Hit ACGCAGAGTAAGCAGTGGTATCAAC 14 0.10164815218180498 No Hit GATACCACTGCTTCCCATGTACTCT 14 0.10164815218180498 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 14 0.10164815218180498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTACTC 45 0.00508817 14.119298 5 CTGCGTT 80 0.004281056 9.40187 11 TCTGCGT 80 0.004281056 9.40187 10 CTCTGCG 85 0.0070580915 8.848819 9 >>END_MODULE