FastQCFastQC Report
Thu 26 May 2016
SRR1926501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13773
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC4203.0494445654541495No Hit
GTACATGGGAAGCAGTGGTATCAAC3842.788063602700937No Hit
GTATCAACGCAGAGTACTTTTTTTT2962.1491323604153054No Hit
GGTATCAACGCAGAGTACTTTTTTT2141.5537646119218762No Hit
CATGTACTCTGCGTTGATACCACTG1651.1979960792855586No Hit
GAGTACATGGGAAGCAGTGGTATCA1571.1399114208959558No Hit
TATCAACGCAGAGTACTTTTTTTTT1561.1326508385972556No Hit
ACGCAGAGTACATGGGAAGCAGTGG1401.0164815218180498No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1270.9220939519349451No Hit
GAGTAAGCAGTGGTATCAACGCAGA1230.8930516227401437No Hit
GCGTTGATACCACTGCTTCCCATGT1090.7914034705583387No Hit
ACTCTGCGTTGATACCACTGCTTCC870.631670659986931No Hit
GTATCAACGCAGAGTACATGGGAAG830.6026283307921295No Hit
TATCAACGCAGAGTACATGGGAAGC820.5953677484934292No Hit
GGTATCAACGCAGAGTACATGGGAA780.5663254192986278No Hit
GCTTACTCTGCGTTGATACCACTGC560.4065926087272199No Hit
GTACTTTTTTTTTTTTTTTTTTTTT540.3920714441298192No Hit
GTGGTATCAACGCAGAGTACATGGG530.38481086183111884No Hit
GCTTCCCATGTACTCTGCGTTGATA520.3775502795324185No Hit
ACATGGGAAGCAGTGGTATCAACGC500.36302911493501777No Hit
CAGTGGTATCAACGCAGAGTACATG470.34124736803891675No Hit
ATACCACTGCTTCCCATGTACTCTG470.34124736803891675No Hit
CTGCTTCCCATGTACTCTGCGTTGA470.34124736803891675No Hit
GAGTACTTTTTTTTTTTTTTTTTTT460.3339867857402164No Hit
GTACTCTGCGTTGATACCACTGCTT440.3194656211428156No Hit
GCAGTGGTATCAACGCAGAGTACAT430.31220503884411527No Hit
ACGCAGAGTAAGCAGTGGTATCAAC420.3049444565454149No Hit
GGGAAGCAGTGGTATCAACGCAGAG400.29042329194801425No Hit
GATACCACTGCTTCCCATGTACTCT380.27590212735061354No Hit
GCAGAGTACTTTTTTTTTTTTTTTT370.2686415450519132No Hit
GCGTTGATACCACTGCTTACTCTGC360.2613809627532128No Hit
AAGCAGTGGTATCAACGCAGAGTAC360.2613809627532128No Hit
CTGCTTACTCTGCGTTGATACCACT350.25412038045451246No Hit
CATGGGAAGCAGTGGTATCAACGCA350.25412038045451246No Hit
CCACTGCTTCCCATGTACTCTGCGT340.2468597981558121No Hit
ATCAACGCAGAGTACTTTTTTTTTT340.2468597981558121No Hit
GCAGAGTACATGGGAAGCAGTGGTA330.23959921585711175No Hit
ATGGGAAGCAGTGGTATCAACGCAG270.1960357220649096No Hit
GTGGTATCAACGCAGAGTACTTTTT270.1960357220649096No Hit
CCATGTACTCTGCGTTGATACCACT260.18877513976620924No Hit
GGTATCAACGCAGAGTAAGCAGTGG260.18877513976620924No Hit
GTTGATACCACTGCTTCCCATGTAC260.18877513976620924No Hit
CTTCCCATGTACTCTGCGTTGATAC230.1669933928701082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA220.1597328105714078No Hit
ACTCTGCGTTGATACCACTGCTTAC220.1597328105714078No Hit
AAAGTACTCTGCGTTGATACCACTG210.15247222827270746No Hit
GTAAGCAGTGGTATCAACGCAGAGT200.14521164597400713No Hit
AAGCAGTGGTATCAACGCAGAGTAA200.14521164597400713No Hit
GTATCAACGCAGAGTAAGCAGTGGT190.13795106367530677No Hit
CAACGCAGAGTACTTTTTTTTTTTT190.13795106367530677No Hit
GTACATGGTAAGCAGTGGTATCAAC190.13795106367530677No Hit
CCACTGCTTACTCTGCGTTGATACC180.1306904813766064No Hit
TACCACTGCTTCCCATGTACTCTGC180.1306904813766064No Hit
ACCATGTACTCTGCGTTGATACCAC170.12342989907790605No Hit
TATCAACGCAGAGTAAGCAGTGGTA170.12342989907790605No Hit
GTGGTATCAACGCAGAGTAAGCAGT160.11616931677920568No Hit
AAAAAGTACTCTGCGTTGATACCAC160.11616931677920568No Hit
TTCCCATGTACTCTGCGTTGATACC160.11616931677920568No Hit
GGAAGCAGTGGTATCAACGCAGAGT150.10890873448050534No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA140.10164815218180498No Hit
ATCAACGCAGAGTACATGGGAAGCA140.10164815218180498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAG908.347038E-510.5171729
GGAAGCA908.347038E-510.5171728
TGGGAAG951.2901856E-410.073536
ATGGGAA1053.0947678E-510.025565
CCATGTA1053.0947678E-510.025562
GGGAAGC951.3912865E-410.07
CATGGGA1251.7715247E-69.9526464
GTACATG2051.9463187E-109.3364421
ACATGGG1853.5179255E-99.3112093
TACATGG2001.3114914E-99.0913612
CCCATGT1201.3385231E-48.7723661
AGCAGTG1451.3033798E-47.8334811
ATGTACT1552.4828225E-47.4089194
TGTACTC1552.4828225E-47.4089195
GCAGTGG1552.825601E-47.32809412
AGTGGTA1450.001160847.1806914
CAGTGGT1450.001160847.1806913
CATGTAC1603.5824694E-47.177393
AAGCAGT1604.0749097E-47.09909110
GTACTCT1655.103937E-46.95989276
Produced by FastQC (version 0.11.5)