FastQCFastQC Report
Thu 26 May 2016
SRR1926500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22290
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA4492.0143562135486763No Hit
GTACATGGGAAGCAGTGGTATCAAC3531.5836698070883806No Hit
CCCATGTACTCTGCGTTGATACCAC3391.5208613728129206No Hit
GAGTACATGGGAAGCAGTGGTATCA1420.6370569762225213No Hit
CATGTACTCTGCGTTGATACCACTG1280.5742485419470614No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA1180.5293853746074473No Hit
GCGTTGATACCACTGCTTCCCATGT1160.5204127411395244No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA1040.46657694033198743No Hit
GTATCAACGCAGAGTACATGGGAAG1010.4531179901301032No Hit
ACGCAGAGTACATGGGAAGCAGTGG910.408254822790489No Hit
CNCATGTACTCTGCGTTGATACCAC840.3768506056527591No Hit
GNACATGGGAAGCAGTGGTATCAAC690.3095558546433378No Hit
ACTCTGCGTTGATACCACTGCTTCC670.30058322117541497No Hit
GGTATCAACGCAGAGTACATGGGAA660.2960969044414536No Hit
TATCAACGCAGAGTACATGGGAAGC580.2602063705697622No Hit
GTATCAACGCAGAGTACTTTTTTTT520.23328847016599372No Hit
GCTTCCCATGTACTCTGCGTTGATA510.2288021534320323No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA500.22431583669807087No Hit
GTGGTATCAACGCAGAGTACATGGG480.21534320323014802No Hit
AAACAAAAAAAAAAAAAAAAAAAAA450.2018842530282638No Hit
CAGTGGTATCAACGCAGAGTACATG420.18842530282637954No Hit
CTGCTTCCCATGTACTCTGCGTTGA420.18842530282637954No Hit
GGTATCAACGCAGAGTACTTTTTTT410.18393898609241813No Hit
CATGGGAAGCAGTGGTATCAACGCA400.1794526693584567No Hit
GTACTCTGCGTTGATACCACTGCTT390.17496635262449528No Hit
GCAGAGTACATGGGAAGCAGTGGTA340.1525347689546882No Hit
TACCACTGCTTCCCATGTACTCTGC330.1480484522207268No Hit
ATACCACTGCTTCCCATGTACTCTG330.1480484522207268No Hit
GCAGTGGTATCAACGCAGAGTACAT310.13907581875280395No Hit
GNGTACATGGGAAGCAGTGGTATCA300.13458950201884254No Hit
GGGAAGCAGTGGTATCAACGCAGAG300.13458950201884254No Hit
NNANNNNAAAANNAAAAAAAAAANA290.1301031852848811No Hit
GATACCACTGCTTCCCATGTACTCT280.1256168685509197No Hit
GAACAAAAAAAAAAAAAAAAAAAAA280.1256168685509197No Hit
ACATGGGAAGCAGTGGTATCAACGC270.12113055181695827No Hit
CCACTGCTTCCCATGTACTCTGCGT270.12113055181695827No Hit
GNAAAAAAAAAAAAAAAAAAAAAAA270.12113055181695827No Hit
ANGCAGAGTACATGGGAAGCAGTGG260.11664423508299686No Hit
CCCNTGTACTCTGCGTTGATACCAC250.11215791834903543No Hit
CCATGTACTCTGCGTTGATACCACT240.10767160161507401No Hit
ANAAAAANAAAAAAAAAAAAAAAAA230.1031852848811126No Hit
CNTGTACTCTGCGTTGATACCACTG230.1031852848811126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA1809.082214E-98.26059714
ATGTACT1856.221544E-68.1672854
CGTTGAT1702.8688373E-77.825829514
TACATGG2303.7014855E-57.8101692
CATGTAC1854.258528E-57.7614573
TGGTATC1701.4248799E-67.63694216
TGCGTTG1805.0080653E-67.602440412
GTGGTAT1752.2060485E-67.418743615
GTACATG2202.4005011E-47.41301871
TTGATAC1657.910703E-67.376591716
GATACCA1708.438854E-67.33997618
GCGTTGA1901.0779981E-57.202311513
GTATCAA2251.5737169E-77.02462918
GTTGATA1751.8147062E-56.95507215
CAGTGGT1606.558582E-46.842196513
TGATACC1752.395294E-56.82099817
ACATGGG2607.4451837E-66.79701573
TGTACTC1908.965719E-46.64264155
GGTATCA2054.261703E-66.599177417
ATACCAC1904.027879E-56.56734719
Produced by FastQC (version 0.11.5)