FastQCFastQC Report
Thu 26 May 2016
SRR1926500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22290
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC9114.087034544638851No Hit
GTACATGGGAAGCAGTGGTATCAAC8683.894122925078511No Hit
GTATCAACGCAGAGTACTTTTTTTT5802.6020637056976224No Hit
GGTATCAACGCAGAGTACTTTTTTT3961.7765814266487217No Hit
CATGTACTCTGCGTTGATACCACTG3731.6733961417676086No Hit
GAGTACATGGGAAGCAGTGGTATCA3671.6464782413638401No Hit
TATCAACGCAGAGTACTTTTTTTTT3531.5836698070883806No Hit
ACGCAGAGTACATGGGAAGCAGTGG3141.4087034544638852No Hit
GCGTTGATACCACTGCTTCCCATGT2451.0991475998205473No Hit
GTATCAACGCAGAGTACATGGGAAG2301.031852848811126No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2271.0183938986092418No Hit
ACTCTGCGTTGATACCACTGCTTCC1820.816509645580978No Hit
GGTATCAACGCAGAGTACATGGGAA1720.7716464782413638No Hit
TATCAACGCAGAGTACATGGGAAGC1680.7537012113055181No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1110.49798115746971733No Hit
GCTTCCCATGTACTCTGCGTTGATA1090.4890085240017945No Hit
CAGTGGTATCAACGCAGAGTACATG1070.48003589053387163No Hit
GTACTCTGCGTTGATACCACTGCTT1050.47106325706594887No Hit
GTGGTATCAACGCAGAGTACATGGG1030.462090623598026No Hit
ATACCACTGCTTCCCATGTACTCTG1030.462090623598026No Hit
CTGCTTCCCATGTACTCTGCGTTGA990.4441453566621804No Hit
CATGGGAAGCAGTGGTATCAACGCA970.4351727231942575No Hit
CCACTGCTTCCCATGTACTCTGCGT940.4217137729923733No Hit
GCAGAGTACATGGGAAGCAGTGGTA930.41722745625841184No Hit
GATACCACTGCTTCCCATGTACTCT890.39928218932256615No Hit
GCAGTGGTATCAACGCAGAGTACAT800.3589053387169134No Hit
CTTCCCATGTACTCTGCGTTGATAC800.3589053387169134No Hit
ACATGGGAAGCAGTGGTATCAACGC770.3454463885150292No Hit
GTACTTTTTTTTTTTTTTTTTTTTT760.34096007178106774No Hit
GCAGAGTACTTTTTTTTTTTTTTTT750.3364737550471063No Hit
ATCAACGCAGAGTACTTTTTTTTTT750.3364737550471063No Hit
GGGAAGCAGTGGTATCAACGCAGAG730.3275011215791835No Hit
GTTGATACCACTGCTTCCCATGTAC680.3050695379093764No Hit
TACCACTGCTTCCCATGTACTCTGC650.29161058770749215No Hit
AAGCAGTGGTATCAACGCAGAGTAC650.29161058770749215No Hit
CCATGTACTCTGCGTTGATACCACT600.2691790040376851No Hit
ATGGGAAGCAGTGGTATCAACGCAG580.2602063705697622No Hit
GTGGTATCAACGCAGAGTACTTTTT510.2288021534320323No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA420.18842530282637954No Hit
CAACGCAGAGTACTTTTTTTTTTTT420.18842530282637954No Hit
ATCAACGCAGAGTACATGGGAAGCA350.15702108568864961No Hit
AAAAAGTACTCTGCGTTGATACCAC310.13907581875280395No Hit
GTACATGGTAAGCAGTGGTATCAAC310.13907581875280395No Hit
ACCATGTACTCTGCGTTGATACCAC280.1256168685509197No Hit
CTGCGTTGATACCACTGCTTCCCAT270.12113055181695827No Hit
GTTATCAACGCAGAGTACTTTTTTT260.11664423508299686No Hit
GGAAGCAGTGGTATCAACGCAGAGT260.11664423508299686No Hit
CTCTGCGTTGATACCACTGCTTCCC250.11215791834903543No Hit
TCAACGCAGAGTACTTTTTTTTTTT250.11215791834903543No Hit
AAAGTACTCTGCGTTGATACCACTG240.10767160161507401No Hit
TTCCCATGTACTCTGCGTTGATACC230.1031852848811126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA1708.731149E-1110.6772042
GCTTCCC1002.203288E-510.41415715
CTTCCCA1053.774181E-59.91824416
ATGTACT1854.110916E-109.8114854
TGTACTC1951.0004442E-109.7982445
CATGTAC1951.0713848E-99.30833153
TACTCTG2251.6370905E-119.2988217
GTACTCT2302.3646862E-119.1379276
TCCCATG950.00173452798.96913118
CCCATGT1951.14232535E-88.7984691
TCTGCGT2501.6370905E-118.71002210
CTCTGCG2552.3646862E-118.577799
TGCGTTG2552.5465852E-118.53923812
CTGCGTT2552.5465852E-118.53923811
TTCCCAT1000.00276494288.52067417
ACTCTGC2501.4733814E-108.3689388
TGCTTCC1303.8666703E-48.0108914
TGATACC2801.9099389E-107.77680617
ACTGCTT1355.801349E-47.714190512
CTGCTTC1355.801349E-47.714190513
Produced by FastQC (version 0.11.5)