FastQCFastQC Report
Thu 26 May 2016
SRR1926499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684644
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGCG250.00519773519.4929948
GTCCTAG602.2696084E-517.7791211
CTAGTAC400.002074473316.3926753
CCTAATA500.0020855316.3784282
GTCCTAA1550.016.3453221
TAGTACC400.002386483416.0524314
GTCCAGA500.002436774616.001211
GTCCTAC4950.015.0853161
TCCTACA5150.014.8412932
TGTAGGA4200.014.6235972
AAAGGCG601.886019E-413.8776075
TCCTAGA700.001230680713.648692
CTGTAGG4600.013.6242181
CTATACT550.001251081413.6202464
GTCTTAC700.001467228513.3343421
GTACTAC600.008180466513.3343411
CTCCTAT600.008180466513.3343411
GTAGGAC4600.013.0666263
TTCTGGA1101.8192492E-512.40792
TAGGACC1900.012.3913524
Produced by FastQC (version 0.11.5)