FastQCFastQC Report
Thu 26 May 2016
SRR1926499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences684644
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12230.17863298298093608No Hit
CTGTAGGACGTGGAATATGGCAAGA11580.16913899778571054No Hit
GTCCTAAAGTGTGTATTTCTCATTT11110.16227411618300897No Hit
CTTTAGGACGTGAAATATGGCGAGG10480.153072253609175No Hit
GTCCTACAGTGTGCATTTCTCATTT10120.147814046424127No Hit
GTATCAACGCAGAGTACTTTTTTTT8920.13028668914063368No Hit
CTGTAGGACCTGGAATATGGCGAGA8280.12093876525610389No Hit
CCCATGTACTCTGCGTTGATACCAC6900.10078230438008659No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA602.4788664E-514.3011621
CTAGGCC500.00145247213.3546214
GTCCTAA3250.012.9077151
TAGCACC603.9478723E-412.7186884
GTCCTAC6600.012.1343191
CTCCTAC550.00298420612.1343181
TAGGACA2450.012.0697764
TAGGACC2950.011.964194
GGCGAGG2850.011.634150519
TAGACTA751.9932014E-411.4468195
TAGCACT751.9932014E-411.4468194
GTCTAAG1001.8429473E-611.4409281
GTAGGAC7300.011.2393983
TTAAAGC600.005687277811.1304893
TAGACAG1201.2003693E-711.1288525
GCACCAT600.005696630611.1280346
GATCTAA600.005715375311.1231261
TGTAGGA8000.011.0915532
CTAAGAC951.2945842E-511.0468023
CTTCTAG951.3041212E-511.0394931
Produced by FastQC (version 0.11.5)