##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 684644 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.422515935288995 31.0 28.0 31.0 23.0 34.0 2 28.903305951706287 31.0 28.0 33.0 23.0 34.0 3 29.255383819912247 31.0 30.0 33.0 25.0 34.0 4 33.01727321060288 35.0 33.0 37.0 27.0 37.0 5 32.6720134843802 35.0 33.0 37.0 26.0 37.0 6 32.72528642623028 35.0 33.0 37.0 26.0 37.0 7 32.54114985306232 35.0 33.0 37.0 25.0 37.0 8 32.51750690869999 35.0 33.0 37.0 25.0 37.0 9 33.77382990284001 37.0 34.0 39.0 23.0 39.0 10 33.71673745771525 37.0 33.0 39.0 23.0 39.0 11 33.922045617868555 37.0 34.0 39.0 25.0 39.0 12 33.729618604705514 37.0 34.0 39.0 23.0 39.0 13 33.86635097948715 37.0 34.0 39.0 25.0 39.0 14 34.533861393658604 39.0 34.0 40.0 22.0 41.0 15 34.64403836154264 39.0 34.0 40.0 24.0 41.0 16 34.621702665911045 39.0 34.0 40.0 24.0 41.0 17 34.543803787077664 39.0 34.0 40.0 23.0 41.0 18 34.509840150501574 39.0 34.0 40.0 22.0 41.0 19 34.557337828126734 39.0 34.0 40.0 22.0 41.0 20 34.54818270517232 39.0 34.0 40.0 21.0 41.0 21 34.45924889431588 39.0 34.0 40.0 19.0 41.0 22 34.394897202049535 39.0 34.0 40.0 18.0 41.0 23 34.048286408702914 39.0 34.0 40.0 17.0 41.0 24 34.12959727975415 39.0 34.0 41.0 18.0 41.0 25 33.931405226657944 39.0 34.0 40.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5745570436447167 1101 2 0.6179350708469649 1101 3 0.658448009458521 1101 4 0.36163551733978494 1101 5 0.37134269859036806 1101 6 0.031994613030775554 1101 7 0.2606278077474684 1101 8 0.4064091461988042 1101 9 0.7514688920000197 1101 10 0.8300243003571168 1101 11 0.671334562058945 1101 12 0.7595126208873424 1101 13 0.7104354605456464 1101 14 0.9621774328151247 1101 15 0.644210627194667 1101 16 0.5715349490468498 1101 17 0.5025803180209394 1101 18 0.6733515017546807 1101 19 0.6756823658513014 1101 20 0.802857344337184 1101 21 0.7281560836102159 1101 22 0.6858530109298613 1101 23 0.7804603540729858 1101 24 0.814347422005774 1101 25 0.7736916699520862 1102 1 0.7130283990368795 1102 2 0.7949302350531475 1102 3 0.8637225949593734 1102 4 0.7235854218376474 1102 5 0.5588952673529732 1102 6 0.297232899911954 1102 7 0.48451874437819953 1102 8 0.5237777588945249 1102 9 0.958170153792274 1102 10 0.9686589072652154 1102 11 0.5876411881540662 1102 12 0.7041788744566944 1102 13 0.5821218983904757 1102 14 0.9660472035319216 1102 15 0.7104041483808103 1102 16 0.7435431343359014 1102 17 0.6683955925425593 1102 18 0.8510536160037674 1102 19 0.7478494729421641 1102 20 0.6775767114323514 1102 21 0.8587743312968357 1102 22 0.8078856484568462 1102 23 0.8893689892974095 1102 24 1.0110122645142 1102 25 1.0422106025143023 1103 1 0.2557843308366152 1103 2 0.1534893477688506 1103 3 0.15649952977550896 1103 4 -0.05777500380400369 1103 5 -0.10623775296844684 1103 6 -0.5758168330392621 1103 7 -0.1179763409159662 1103 8 -0.25984807035935376 1103 9 0.10580126923142785 1103 10 0.16587467435746817 1103 11 0.0030954633089237404 1103 12 0.023556220858893084 1103 13 0.01564388142588058 1103 14 0.2094199300010544 1103 15 0.23118106274183248 1103 16 0.2202633909598859 1103 17 0.03274350184751995 1103 18 0.009262215771016713 1103 19 0.17439187967598713 1103 20 0.1800937859715077 1103 21 -0.10937267666823658 1103 22 -0.038673984403125417 1103 23 0.3136635253718225 1103 24 -0.012817940526382188 1103 25 0.07333013268961253 1104 1 0.6080323500066527 1104 2 0.5689220303356137 1104 3 0.4883217501521955 1104 4 0.3131733961750882 1104 5 0.2739656172525571 1104 6 -0.15521362404818717 1104 7 -0.04237296367873711 1104 8 0.038025656249956796 1104 9 0.4619955555921109 1104 10 0.4263318886450733 1104 11 0.0051409069525476525 1104 12 0.14937570490879892 1104 13 0.1217635309297691 1104 14 0.45617331622334234 1104 15 0.3105037538497726 1104 16 0.26987739133074484 1104 17 0.11054625005891694 1104 18 0.12095260275864916 1104 19 0.24804861313756987 1104 20 0.29252517196898964 1104 21 0.3248692097505739 1104 22 0.22130140154281008 1104 23 0.2052421253204173 1104 24 0.13716598659654267 1104 25 0.37451651864055435 1105 1 0.6794393474434308 1105 2 0.7236650821497328 1105 3 0.7831040122105009 1105 4 0.5984653369869406 1105 5 0.8487433079569229 1105 6 0.20344038432174472 1105 7 0.36716252689828366 1105 8 0.41854733480758455 1105 9 0.872230354479747 1105 10 0.8550378145347182 1105 11 0.6875392895639862 1105 12 0.8248905264355244 1105 13 0.7500872861881902 1105 14 0.9813368509683542 1105 15 0.9887545851287172 1105 16 1.1066963820411004 1105 17 0.6595474908549903 1105 18 0.8131310978036268 1105 19 0.8056088978676144 1105 20 0.9326487345774908 1105 21 1.0162722072526549 1105 22 1.063709416650049 1105 23 1.4306642952985982 1105 24 1.0747717671824404 1105 25 1.3551760180773016 1106 1 1.0472533830333361 1106 2 1.1850218876456537 1106 3 0.9058975335119648 1106 4 0.9092210261239089 1106 5 1.1705731347781665 1106 6 0.13618452820801252 1106 7 0.20942315377677545 1106 8 0.547644229039733 1106 9 0.39792688613376725 1106 10 0.6033322661353111 1106 11 0.20994995699397379 1106 12 0.37429775891489214 1106 13 0.2603503938643499 1106 14 0.5936466167238024 1106 15 0.4576432354793738 1106 16 0.5994209657918717 1106 17 0.45513811153148254 1106 18 0.5388180879281421 1106 19 0.5440576837079902 1106 20 0.729718292327604 1106 21 0.7191310880312471 1106 22 0.6904447823291662 1106 23 0.9325010397287272 1106 24 0.7473124379519689 1106 25 0.8599169655511787 1107 1 1.181798366091673 1107 2 1.0400241434468995 1107 3 0.9634383264691024 1107 4 0.9246683032196543 1107 5 0.959730470630582 1107 6 0.8993935344779445 1107 7 1.0101763664743544 1107 8 1.2450248534931418 1107 9 1.38359229457933 1107 10 0.7743140622823219 1107 11 0.7853519328377203 1107 12 0.8232445444973422 1107 13 0.7842823943565236 1107 14 1.1584218502753032 1107 15 1.0604674192563905 1107 16 0.8585596069395436 1107 17 0.939946844723778 1107 18 1.06150377477001 1107 19 1.245613855805118 1107 20 0.8668134553869677 1107 21 0.8794451273179078 1107 22 1.0158146741962426 1107 23 1.0116439410992797 1107 24 0.9653359591452499 1107 25 1.2463932716997945 1108 1 1.2195692257613153 1108 2 1.4194545447729396 1108 3 1.1696785241160121 1108 4 1.1782469816919843 1108 5 -0.10487195379621994 1108 6 0.8428178930958907 1108 7 1.1777330037492604 1108 8 1.2429961925929263 1108 9 1.547710568001385 1108 10 1.5739500310826458 1108 11 1.2576220922456187 1108 12 1.3536849488132603 1108 13 1.3322673715127777 1108 14 1.5995539663246774 1108 15 1.6963103298274547 1108 16 1.5543194827283173 1108 17 1.5869559140313356 1108 18 1.5229775765880618 1108 19 1.6444037405635399 1108 20 1.6077769316226593 1108 21 1.5600824966417193 1108 22 1.7789911349107683 1108 23 1.8268141807618932 1108 24 1.6216503792918076 1108 25 1.8345357684083012 1109 1 0.3893108822642546 1109 2 -0.2758165495834213 1109 3 0.3720041699747547 1109 4 0.5755388100543328 1109 5 -0.6100914903302055 1109 6 0.2399628093837265 1109 7 0.740680669787622 1109 8 0.7130593925042277 1109 9 1.0491686010427372 1109 10 -1.1958636954945803 1109 11 0.33203444394757753 1109 12 0.6141794603999102 1109 13 0.7332403175156585 1109 14 1.120648322683472 1109 15 0.997859836424638 1109 16 0.8729911758206796 1109 17 0.9659031365615505 1109 18 1.1069613885406113 1109 19 0.9519239756228544 1109 20 1.1057950045797469 1109 21 1.131789448631963 1109 22 1.1456116028118686 1109 23 1.1332561366328306 1109 24 0.8095869576264363 1109 25 1.0506628334931207 1110 1 0.5000940500024207 1110 2 -0.5753489867546655 1110 3 -0.031283381164929125 1110 4 0.539697196962571 1110 5 0.8347214845382922 1110 6 0.6845858368530386 1110 7 0.9354936446561197 1110 8 1.0823171365135806 1110 9 1.1515064151865175 1110 10 0.7129031075445766 1110 11 0.6255031756834057 1110 12 0.972683259347896 1110 13 0.9616885933777297 1110 14 1.305627866809715 1110 15 1.1800778375935508 1110 16 0.7581752786845044 1110 17 0.8299446327625972 1110 18 0.9545651290432389 1110 19 1.0871226898250796 1110 20 1.0069785229900319 1110 21 0.8387298343712999 1110 22 1.0762661907721238 1110 23 1.2686447982342344 1110 24 1.3838417793681046 1110 25 1.426437234428242 1111 1 -0.09614888785068842 1111 2 -0.046563676020202394 1111 3 0.10553865273337593 1111 4 0.03171697097386783 1111 5 0.32554069357784243 1111 6 -0.1747785699266231 1111 7 0.2588128536956731 1111 8 0.42593295572261525 1111 9 0.5387663021921654 1111 10 0.6397110171490965 1111 11 0.04502294969470455 1111 12 0.07173693166428308 1111 13 -0.02581140662226744 1111 14 0.3011080927984153 1111 15 0.48955523872349005 1111 16 0.5988532447861985 1111 17 0.5251847457569525 1111 18 0.33224874486244715 1111 19 0.25781478022055637 1111 20 0.1897892574533131 1111 21 0.31210554058598916 1111 22 0.3292322924670401 1111 23 0.6661871711155882 1111 24 0.41312144038505494 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0.9454710075954154 2202 22 0.8293394047206846 2202 23 0.9118041376965635 2202 24 0.683851945954892 2202 25 0.8165967046038389 2203 1 -0.04322111994068223 2203 2 -0.004535848903564954 2203 3 -0.066277510676926 2203 4 -0.3503064156454343 2203 5 -0.057511408257568064 2203 6 -0.07869952907404354 2203 7 0.1749659028371724 2203 8 0.0936712624129683 2203 9 0.5795507900548031 2203 10 0.6378075239578607 2203 11 0.39624556662779753 2203 12 0.402049246643557 2203 13 0.2925056448874983 2203 14 0.645453859524288 2203 15 0.7124874471308331 2203 16 0.4677697634362872 2203 17 0.45909386357814697 2203 18 0.3035927353893868 2203 19 0.2817564616888859 2203 20 0.4326385765770553 2203 21 0.3908281575190813 2203 22 0.36409019655229713 2203 23 0.7168354837266051 2203 24 0.6531436428717328 2203 25 0.6509262009213046 2204 1 0.3286828771906585 2204 2 0.14150447339525485 2204 3 0.08552368267349664 2204 4 0.030943517880054117 2204 5 0.062231597484313284 2204 6 0.17473169525730015 2204 7 0.3644152203908533 2204 8 0.327709402828404 2204 9 0.840093647501547 2204 10 0.6690680174342418 2204 11 0.3810779824862607 2204 12 0.5391713485448051 2204 13 0.2065560014187895 2204 14 0.7200701686467212 2204 15 0.440167585458596 2204 16 0.5364922524850826 2204 17 0.4177474774364498 2204 18 0.409038023448133 2204 19 0.4516592341698029 2204 20 0.3301136087507146 2204 21 0.49071617645710575 2204 22 0.4715547903284616 2204 23 0.9249083002858214 2204 24 0.8451980015484395 2204 25 0.5109589899486835 2205 1 0.28724617387770834 2205 2 0.4505350894118969 2205 3 0.3646641671366666 2205 4 0.13495771171461257 2205 5 0.07276291580006244 2205 6 0.030540360114528653 2205 7 0.08855565204957827 2205 8 -0.06472548049137572 2205 9 0.5453300882003944 2205 10 0.37796204595568383 2205 11 0.2372245957852428 2205 12 0.4023233514173654 2205 13 -0.016294945716921916 2205 14 0.5434949240741389 2205 15 0.5112733457193741 2205 16 0.3902112694775539 2205 17 0.4126641696252591 2205 18 0.5030306378665657 2205 19 0.560746878985988 2205 20 0.38056086239158304 2205 21 0.43057261877528674 2205 22 0.24991130481271995 2205 23 0.7353847019797897 2205 24 0.7519383128130386 2205 25 0.5902825298177987 2206 1 0.6188787746666016 2206 2 0.738491649014378 2206 3 0.7740967440196833 2206 4 0.7604686998812014 2206 5 0.9730856451684318 2206 6 0.7301903097414311 2206 7 0.9006212907634463 2206 8 0.8787268699688155 2206 9 1.3072241168532983 2206 10 1.4137013352680512 2206 11 1.103536089390417 2206 12 1.1432894904471311 2206 13 0.8183275975271087 2206 14 1.189213210364116 2206 15 1.1259003286266704 2206 16 1.2822557344127503 2206 17 1.1775464331705052 2206 18 1.1340468084862394 2206 19 1.0705568272468398 2206 20 1.1200209310356044 2206 21 1.1989485558003707 2206 22 1.1888795953716027 2206 23 1.5234693287919399 2206 24 1.3980903200531074 2206 25 1.3920993766323377 2207 1 -0.9350503080678969 2207 2 -0.33695214719814714 2207 3 -0.17780930048299837 2207 4 -0.025164816828969094 2207 5 -0.12155621536117422 2207 6 -0.20275893751225027 2207 7 -0.24480243452487827 2207 8 -0.0012767351712881236 2207 9 0.1132065695271649 2207 10 -0.08643351083753714 2207 11 -0.12231623159184579 2207 12 -0.33739631812686355 2207 13 -0.46667397972168345 2207 14 -5.265057320735419 2207 15 -2.4844443435655634 2207 16 -1.2739120016632626 2207 17 -1.0421147529899173 2207 18 -0.6775256912277783 2207 19 -0.4997562306399246 2207 20 -0.41351692466866297 2207 21 -0.3654111678517751 2207 22 -0.3492347083683853 2207 23 -0.046607315099947755 2207 24 -0.4730377074846217 2207 25 -0.4059240359794103 2208 1 0.34248415880034244 2208 2 0.6068126596587149 2208 3 0.5215492078545658 2208 4 0.46823312943099893 2208 5 0.44407731072665513 2208 6 0.42514519864257494 2208 7 0.5612294552072683 2208 8 0.6344891193921498 2208 9 0.8171621843917976 2208 10 0.733379247537421 2208 11 0.4484878962675509 2208 12 0.5090232230377509 2208 13 0.338350047638869 2208 14 0.8018378295719373 2208 15 0.7450471594949306 2208 16 0.5895491009238043 2208 17 0.20815125605145113 2208 18 0.5906016247164985 2208 19 0.2526512409972099 2208 20 0.30040757905872795 2208 21 0.5505763511150477 2208 22 0.5666247866030858 2208 23 0.9758001500053481 2208 24 0.7114971245132793 2208 25 0.7772946309642066 2209 1 -0.389061981046801 2209 2 0.06857926280198257 2209 3 0.2851570060781441 2209 4 0.6517927285496299 2209 5 0.602570424852857 2209 6 0.6040250321316947 2209 7 0.8125192573549072 2209 8 0.6796264948878559 2209 9 0.7925027230040556 2209 10 0.388530342477857 2209 11 0.488885156670122 2209 12 0.6152174919844668 2209 13 0.592477615676188 2209 14 0.871491168841871 2209 15 0.7463205331774674 2209 16 0.7775839480452049 2209 17 0.9903205376380271 2209 18 0.8177261605285437 2209 19 0.9969156950290241 2209 20 0.8003224192600555 2209 21 0.6918725503058312 2209 22 0.6930830643366832 2209 23 0.8996605844946615 2209 24 0.9374481985325716 2209 25 0.8960523171389454 2210 1 -0.30246006051920205 2210 2 -0.19079728636184612 2210 3 0.6322861134905331 2210 4 0.7127765277605747 2210 5 0.9083430943165283 2210 6 0.7991757237021915 2210 7 0.9427717641296525 2210 8 0.9278443130633391 2210 9 1.2761578072706499 2210 10 1.280888302467659 2210 11 1.092211315345672 2210 12 0.9050079381278096 2210 13 0.8811253431468913 2210 14 1.2186519524475372 2210 15 1.1227754603301392 2210 16 1.185833854204482 2210 17 1.273806629413457 2210 18 1.1740769997100955 2210 19 1.3569029611993244 2210 20 1.4491221291061294 2210 21 1.6274217917401899 2210 22 1.4281473432518936 2210 23 1.5033201351045093 2210 24 1.3738162695632639 2210 25 1.2535316207061626 2211 1 -1.1494384383468947 2211 2 -0.42966641974466313 2211 3 0.18443094695404127 2211 4 0.19573477599430333 2211 5 0.6334361985398971 2211 6 0.39715225319071834 2211 7 0.6143309506016834 2211 8 0.39164807539570745 2211 9 0.677730108380139 2211 10 0.4825860901204919 2211 11 0.42077017678168716 2211 12 0.6372696228493382 2211 13 0.2563470872481304 2211 14 0.5020421266571446 2211 15 0.4119443222027712 2211 16 0.6083103376058148 2211 17 0.46904493256371893 2211 18 0.5288044950083943 2211 19 0.668753192362999 2211 20 0.8041690005940012 2211 21 0.5782249217885536 2211 22 0.6122883344985652 2211 23 0.9739892726917745 2211 24 0.7305513878456509 2211 25 0.8648726287706126 2212 1 0.671839304701539 2212 2 -0.25240836266870303 2212 3 0.28398778988142226 2212 4 0.24253668032900322 2212 5 0.32120372718365076 2212 6 0.3844834645874542 2212 7 0.5146643523332699 2212 8 0.6255605669851079 2212 9 0.6804238862066967 2212 10 0.35502890997380376 2212 11 0.3837468370698147 2212 12 0.5547773494662778 2212 13 0.42422265332323406 2212 14 0.36187103557770683 2212 15 0.4777863922203096 2212 16 0.0029404382466893253 2212 17 0.5915812035226153 2212 18 0.5828391172511829 2212 19 0.4786935268145669 2212 20 0.45936464346602435 2212 21 0.6630705723752683 2212 22 0.7732604486886601 2212 23 1.0584714581274 2212 24 0.9660982796221163 2212 25 0.4847039873031349 2213 1 0.5047777593417813 2213 2 0.21311337688469933 2213 3 -0.27906942905825005 2213 4 -0.843692798581472 2213 5 -1.5596508026896068 2213 6 -0.6578043732372336 2213 7 -0.24432168054645587 2213 8 -0.2316489981884544 2213 9 -0.7894826429555124 2213 10 -0.30535065114041515 2213 11 -0.31776997468050183 2213 12 -0.5283028281539615 2213 13 -0.5409891086670626 2213 14 -7.13352352031421 2213 15 -3.203663683704292 2213 16 -1.893551818504939 2213 17 -1.2833019468753122 2213 18 -5.512314708561764 2213 19 -3.347690737468504 2213 20 -5.770044400449969 2213 21 -2.5904707333510437 2213 22 -2.0533139587500955 2213 23 -4.815862488085969 2213 24 -2.6851331847366566 2213 25 -2.2492901598252146 2214 1 0.29011405210790286 2214 2 0.1807551645595069 2214 3 -1.6999116578256306 2214 4 -2.0095097371006645 2214 5 -0.29003901656708564 2214 6 -0.35429720346699156 2214 7 -0.49951530365422414 2214 8 -0.5428662161200322 2214 9 -5.337848604640133 2214 10 -1.5085395004492455 2214 11 -0.7725650420237216 2214 12 -1.1561460911100454 2214 13 -1.3307544708044645 2214 14 -2.6529291742823276 2214 15 -2.1962056308417246 2214 16 -1.3665711858556264 2214 17 -1.5622738463755539 2214 18 -0.9830721661106381 2214 19 -4.088329112118984 2214 20 -2.065164158695758 2214 21 -4.105104604341548 2214 22 -5.959328784137515 2214 23 -5.969185499691864 2214 24 -5.841676696260908 2214 25 -3.471504654191609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22645.0 3 18.0 4 455.0 5 2767.0 6 3446.0 7 2682.0 8 2056.0 9 1582.0 10 1414.0 11 1393.0 12 1215.0 13 1280.0 14 1291.0 15 1307.0 16 1323.0 17 1412.0 18 1518.0 19 1816.0 20 2362.0 21 3171.0 22 3984.0 23 4883.0 24 6092.0 25 7102.0 26 8188.0 27 9309.0 28 10352.0 29 12668.0 30 15714.0 31 19564.0 32 25102.0 33 32611.0 34 42429.0 35 56543.0 36 77225.0 37 113414.0 38 184311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.98699772233544 19.579728493028124 8.952625161907157 30.480648622729277 2 13.507475988300232 21.500807938544074 39.31373437084525 25.677981702310444 3 17.72377156482304 25.0561036046566 29.32538337463182 27.89474145588854 4 11.877895653469817 17.716029811624544 37.55893581675874 32.8471387181469 5 12.877145411726204 38.64838330771793 35.450499938639915 13.023971341915955 6 31.73010380622837 38.29100932496628 17.647058823529413 12.331828045275937 7 27.983027105986913 31.756407648048103 22.08320465100621 18.177360594958774 8 24.931751480242696 38.18125928582407 19.996633316987843 16.890355916945392 9 26.202626147292605 15.538770073046127 19.84790596226194 38.410697817399324 10 14.386531297932947 29.970199401066395 34.38127236870937 21.261996932291286 11 34.14506250830742 22.783142402673565 24.5383996295804 18.533395459438616 12 23.118304699527624 25.116779770442665 31.74627750788026 20.01863802214945 13 28.92379105053137 22.372210959272266 25.639310356915416 23.064687633280943 14 20.457053488559048 22.74188244063932 27.43475623570338 29.36630783509826 15 22.44308637899958 31.886234104645695 22.91793863582586 22.752740880528865 16 21.742235906903915 28.552669632763 27.970015745823268 21.73507871450982 17 21.21729553501525 29.30241186300057 27.979042266029424 21.501250335954754 18 21.1561860496368 28.112670954247143 29.789238591729926 20.94190440438613 19 23.121730912766402 27.444046664417286 27.277534094909246 22.156688327907066 20 23.187065827680694 27.977056421222375 27.98728083244235 20.848596918654586 21 22.307684442841015 27.116175875602323 27.049571820642292 23.526567860914373 22 22.745543669410672 28.789998889934036 26.895145506277718 21.569311934377573 23 21.900432925724903 28.458147592033235 27.490199286052313 22.151220196189552 24 22.82310222539013 27.388832736429443 28.35064646736114 21.437418570819286 25 22.283989927612012 27.970302814309335 27.841330676964965 21.904376581113688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 111.0 1 111.0 2 121.5 3 132.0 4 132.0 5 132.0 6 259.0 7 386.0 8 386.0 9 386.0 10 662.0 11 938.0 12 938.0 13 938.0 14 1689.0 15 2440.0 16 2440.0 17 2440.0 18 4573.5 19 6707.0 20 6707.0 21 6707.0 22 11070.5 23 15434.0 24 15434.0 25 15434.0 26 22837.0 27 30240.0 28 30240.0 29 30240.0 30 40603.5 31 50967.0 32 50967.0 33 50967.0 34 59679.5 35 68392.0 36 68392.0 37 68392.0 38 74824.5 39 81257.0 40 81257.0 41 81257.0 42 85654.5 43 90052.0 44 90052.0 45 90052.0 46 91933.0 47 93814.0 48 93814.0 49 93814.0 50 89653.0 51 85492.0 52 85492.0 53 85492.0 54 74458.0 55 63424.0 56 63424.0 57 63424.0 58 53650.0 59 43876.0 60 43876.0 61 43876.0 62 35432.0 63 26988.0 64 26988.0 65 26988.0 66 20474.0 67 13960.0 68 13960.0 69 13960.0 70 10137.5 71 6315.0 72 6315.0 73 6315.0 74 4399.5 75 2484.0 76 2484.0 77 2484.0 78 1690.0 79 896.0 80 896.0 81 896.0 82 568.0 83 240.0 84 240.0 85 240.0 86 157.0 87 74.0 88 74.0 89 74.0 90 47.0 91 20.0 92 20.0 93 20.0 94 12.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08909739952442437 2 0.027167403789414644 3 0.028628016896372423 4 0.031111059178200638 5 0.023369809711324425 6 0.38034365305180506 7 0.20989010346983253 8 0.21602467851905516 9 0.2211368243934074 10 0.0137297632054031 11 0.002190919660436665 12 0.0032133488353071086 13 0.0 14 0.0699633678232775 15 0.0014606131069577765 16 0.002629103592523998 17 0.004089716699481775 18 0.004527900631569107 19 0.0016066744176535543 20 0.0011684904855662212 21 1.4606131069577765E-4 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 684644.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.87489200161087 #Duplication Level Percentage of deduplicated Percentage of total 1 92.94799069456464 79.8189866266247 2 5.4721123416598205 9.398341127214382 3 0.7946513118171789 2.0472178674371593 4 0.3021420726896518 1.0378567144546684 5 0.13276206680316038 0.5700464074316023 6 0.0735449766167497 0.3789400154534627 7 0.05175015310925975 0.31108271665271553 8 0.03885897099215898 0.26696079497963054 9 0.027119907459090264 0.20960272117287515 >10 0.1326279763506214 2.0636926531719735 >50 0.01449937822210796 0.8528348072758712 >100 0.010063840476971347 1.6429181848454044 >500 0.0010234414028354921 0.588263144121526 >1k 8.528678356962433E-4 0.813256219164022 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1223 0.17863298298093608 No Hit CTGTAGGACGTGGAATATGGCAAGA 1158 0.16913899778571054 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1111 0.16227411618300897 No Hit CTTTAGGACGTGAAATATGGCGAGG 1048 0.153072253609175 No Hit GTCCTACAGTGTGCATTTCTCATTT 1012 0.147814046424127 No Hit GTATCAACGCAGAGTACTTTTTTTT 892 0.13028668914063368 No Hit CTGTAGGACCTGGAATATGGCGAGA 828 0.12093876525610389 No Hit CCCATGTACTCTGCGTTGATACCAC 690 0.10078230438008659 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.4606131069577765E-4 0.0 9 0.0 0.0 0.0 1.4606131069577765E-4 0.0 10 0.0 0.0 0.0 2.921226213915553E-4 0.0 11 0.0 0.0 0.0 2.921226213915553E-4 0.0 12 0.0 0.0 0.0 2.921226213915553E-4 0.0 13 0.0 0.0 0.0 2.921226213915553E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAA 60 2.4788664E-5 14.301162 1 CTAGGCC 50 0.001452472 13.354621 4 GTCCTAA 325 0.0 12.907715 1 TAGCACC 60 3.9478723E-4 12.718688 4 GTCCTAC 660 0.0 12.134319 1 CTCCTAC 55 0.002984206 12.134318 1 TAGGACA 245 0.0 12.069776 4 TAGGACC 295 0.0 11.96419 4 GGCGAGG 285 0.0 11.6341505 19 TAGACTA 75 1.9932014E-4 11.446819 5 TAGCACT 75 1.9932014E-4 11.446819 4 GTCTAAG 100 1.8429473E-6 11.440928 1 GTAGGAC 730 0.0 11.239398 3 TTAAAGC 60 0.0056872778 11.130489 3 TAGACAG 120 1.2003693E-7 11.128852 5 GCACCAT 60 0.0056966306 11.128034 6 GATCTAA 60 0.0057153753 11.123126 1 TGTAGGA 800 0.0 11.091553 2 CTAAGAC 95 1.2945842E-5 11.046802 3 CTTCTAG 95 1.3041212E-5 11.039493 1 >>END_MODULE