FastQCFastQC Report
Thu 26 May 2016
SRR1926498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704645
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT7410.1051593355519446No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC451.09418E-519.3527073
CTGTAGG4950.016.0839271
CCTTATA851.3203244E-615.9868722
ATAGGAC752.0616426E-715.9659833
GTTTTAA758.953693E-615.9230891
GTTTAGG500.002517933515.9230881
GCCTTAG604.7413388E-415.4807811
ATTAGAG505.343261E-415.2402563
TGTAGGA5200.015.1567832
AAGCCTG507.6920784E-414.52564055
CTAGCAC601.2881577E-414.5145293
GTCCTAG1101.0567237E-714.4755341
GTTCTAA658.700487E-414.2899511
CTATTAT658.700487E-414.2899511
TAGGACC2400.014.2313134
CTAGGTA450.00531867214.2313134
CACTATG450.00740945413.5367757
GTCCTAC4450.013.4183331
GTAGGAC5000.013.2807953
GTCCTGT700.001522556513.269241
Produced by FastQC (version 0.11.5)