FastQCFastQC Report
Thu 26 May 2016
SRR1926498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704645
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA15150.21500188037948184No Hit
GTCCTACAGTGGACATTTCTAAATT14760.2094671785083269No Hit
CTTTAGGACGTGAAATATGGCGAGG13000.18449006237183263No Hit
GTCCTAAAGTGTGTATTTCTCATTT12480.17711045987695934No Hit
GTCCTACAGTGTGCATTTCTCATTT10890.15454590609455823No Hit
GTATCAACGCAGAGTACTTTTTTTT10790.15312675176862106No Hit
CCCATGTACTCTGCGTTGATACCAC9880.14021244740259278No Hit
CTGTAGGACCTGGAATATGGCGAGA8900.1263047350084085No Hit
GTACATGGGAAGCAGTGGTATCAAC8550.12133769486762838No Hit
CTGAAGGACCTGGAATATGGCGAGA7170.10175336516969537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2600.015.0432114
GTCTAGG400.005129241414.3104181
GGACCTG4800.013.1169466
AATCCCG1207.730705E-1012.628319
AGGACCT5300.012.4194755
GGATAAA1001.3608951E-712.4023631
ATAAATC550.002968332312.1430423
AGGACGA550.002971513412.1413055
GCCTCGA550.00313435512.05514116
GTCTTAT802.7398388E-511.9253481
TTATACC657.7389163E-411.7410424
ACTGTTC2000.011.4010698
GTCCTAC8200.011.2854521
GTCCTAA3900.011.2526371
TCACGTC1201.2689634E-711.0843728
TAGACTT951.2959712E-511.0458495
GTATTAG700.001437960510.9031761
GTAGGAC8000.010.8528433
GGCGAGG3500.010.82425719
TGTAGGA8500.010.7787562
Produced by FastQC (version 0.11.5)