FastQCFastQC Report
Thu 26 May 2016
SRR1926497_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926497_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804461
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8850.11001154810488015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGACC300.00855760517.879974
CCCTGTA806.483533E-717.1140162
ACTGCGT350.001816076516.7233568
ACTGTCG350.001816076516.7233568
GTTTAAC500.00241832416.0202961
CTGTAGG5100.015.4444041
TGTAGGA4950.014.9358682
TAGGACC2100.014.8148334
TGCACTG1201.0913936E-1114.7733855
GTCCTAA2200.014.5639071
GTCCTAC6000.014.2402631
GTCCTAG955.0457493E-614.0528921
TCCTACA6450.013.5850792
GTCATAT600.008119089513.3502461
GTATGAG802.6182068E-413.3502461
GTAGGAC5150.013.1932253
CCTGTAC755.4525404E-513.1506653
TAGGACA1650.013.0036154
CTTTAAC600.00201526212.785373
TAATAGT851.530254E-512.6211554
Produced by FastQC (version 0.11.5)