FastQCFastQC Report
Thu 26 May 2016
SRR1926497_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926497_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804461
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA17500.21753695952942403No Hit
GTCCTACAGTGGACATTTCTAAATT16680.2073437991400453No Hit
GTCCTACAGTGTGCATTTCTCATTT12780.15886413387348797No Hit
GTCCTAAAGTGTGTATTTCTCATTT12670.15749675869930302No Hit
CTTTAGGACGTGAAATATGGCGAGG12360.15364324684478178No Hit
GTATCAACGCAGAGTACTTTTTTTT11930.14829805298205878No Hit
CTGTAGGACCTGGAATATGGCGAGA10780.1340027670701252No Hit
CCCATGTACTCTGCGTTGATACCAC9860.12256653834057836No Hit
GTACATGGGAAGCAGTGGTATCAAC9500.11809149231597306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.014.2558854
ATAGGAC1204.7293724E-1113.5121453
ATGTCGA500.001530484813.26271417
GTAATAC655.2458847E-513.206443
GTAGGAC10750.013.042643
TGTAGGA10900.012.9514682
CTGTAGG11100.012.4548531
TAAGACT1155.005859E-912.4400714
AGGACCT6950.012.35057455
AAGGCGT550.002977309512.1384946
CCCTTAT550.00298429612.1346981
CCGTGTG550.003059221212.0945999
TAGGACA2700.012.0100444
AGGACGT9200.011.9217355
GTATTAC802.7583194E-511.9180071
GTCTAGA802.7583194E-511.9180071
GTCTTAC802.7583194E-511.9180071
TAGGACG9350.011.8324824
GTATTAT907.153996E-611.6531621
TTAGGAC5600.011.581843
Produced by FastQC (version 0.11.5)