FastQCFastQC Report
Thu 26 May 2016
SRR1926496_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926496_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596174
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8110.1360341108468333No Hit
GTCCTAAAGTGTGTATTTCTCATTT6560.11003498978486147No Hit
CTGTAGGACGTGGAATATGGCAAGA6300.10567384689704684No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC350.00596709119.0304781
GTCTAGA604.5994454E-415.5415561
TGGACTG652.3422459E-514.3848795
TGTAGGA5450.014.2642282
TAGGGTT450.00635952213.8521075
CTGTAGG5450.013.6879751
AACAGGC602.3082178E-413.5502647
CTAGAAG755.7688012E-513.0702073
TCTAGGC500.00933765313.0702063
GTCCTAC5150.012.9333341
CCTGTAC600.002108836512.7071453
GTAGGAC5750.012.5019363
TGTACTG758.9815585E-512.4668965
TACACAG1503.6379788E-1112.4668965
TCCTACA5800.012.2277152
TTGGAGA705.6173716E-412.1953074
CTGTACT1058.506013E-712.1953064
GTCCTAA2750.012.1103041
ACAGGCC656.4604694E-411.994638
TTCTATA800.003374489311.9337782
Produced by FastQC (version 0.11.5)