FastQCFastQC Report
Thu 26 May 2016
SRR1926495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710584
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9240.13003388761919774No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG450.001239948817.7019331
GCTCTAG704.6231635E-617.0697211
TTATATA901.3035424E-716.6202222
GTCCTAA3200.016.1806721
ATAGGAC1151.8189894E-1216.1044883
GTCATAG500.002508877515.9317391
TAGGACC2700.015.430174
TGTAGGA6250.015.0125932
GTCCTAG801.6416741E-514.9360051
CTAAGGT651.8019155E-514.7910514
CTGTAGG6100.014.5823291
TATAGGA751.294452E-414.5049212
GTATTGA550.00473161514.4833981
GTATTGG658.665037E-414.2977141
CTAAGGC703.0124285E-514.00684453
TCTAAGG703.0124285E-514.00684453
TAGGACA2100.013.7345464
CTTAGAC806.9885227E-613.6177653
TACTCTC450.007416278613.5349257
CTGCTAG700.001516379213.2764491
Produced by FastQC (version 0.11.5)