##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 710584 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.721454465622642 27.0 10.0 31.0 2.0 31.0 2 16.809755074699122 22.0 2.0 31.0 2.0 31.0 3 20.484753385947332 25.0 15.0 31.0 2.0 31.0 4 22.569155792981547 28.0 7.0 35.0 2.0 37.0 5 23.10134621663308 30.0 7.0 35.0 2.0 37.0 6 24.050580930614817 31.0 8.0 35.0 2.0 37.0 7 23.663565461648446 30.0 8.0 35.0 2.0 37.0 8 23.90534827691026 31.0 12.0 35.0 2.0 37.0 9 26.72105197978001 32.0 17.0 37.0 2.0 39.0 10 27.499527149499567 32.0 25.0 38.0 2.0 39.0 11 24.548928768449613 30.0 8.0 37.0 2.0 39.0 12 25.749379383718182 31.0 17.0 37.0 2.0 39.0 13 22.33453891447035 28.0 2.0 37.0 2.0 39.0 14 25.741273375139322 31.0 17.0 38.0 2.0 40.0 15 26.863706472422685 31.0 17.0 38.0 2.0 40.0 16 27.221441799984238 32.0 19.0 39.0 2.0 40.0 17 26.544678743118336 31.0 17.0 38.0 2.0 40.0 18 26.590176530853494 31.0 17.0 38.0 2.0 40.0 19 26.996770262206862 31.0 19.0 39.0 2.0 40.0 20 27.279975344223907 31.0 23.0 39.0 2.0 40.0 21 25.081168447361605 30.0 10.0 38.0 2.0 40.0 22 26.04284926201547 31.0 11.0 38.0 2.0 40.0 23 26.054970277968543 31.0 8.0 38.0 2.0 40.0 24 25.092260732017607 31.0 2.0 39.0 2.0 40.0 25 24.889331873501234 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.282277127880484 1101 2 11.034725164717997 1101 3 7.574467316300396 1101 4 9.03247591191112 1101 5 8.545572450484215 1101 6 8.278119918550864 1101 7 7.937446948112797 1101 8 6.346871162009979 1101 9 6.360314447154078 1101 10 5.877131000803004 1101 11 8.910632889137702 1101 12 6.501216451056738 1101 13 11.051855302315836 1101 14 8.4065493257365 1101 15 7.501715306787048 1101 16 7.038739541002499 1101 17 3.3764491214313175 1101 18 5.694842060567396 1101 19 6.023722621749677 1101 20 4.003288987019133 1101 21 1.4442732377297673 1101 22 5.023760093716728 1101 23 6.3014743160909745 1101 24 7.814927957813698 1101 25 8.31597248251352 1102 1 5.795816429311586 1102 2 10.960282538321742 1102 3 7.03076775585545 1102 4 8.326476223944923 1102 5 8.043393371448115 1102 6 7.070036566843637 1102 7 6.759405875696455 1102 8 6.987753779277313 1102 9 5.946428040352256 1102 10 5.455963375470503 1102 11 8.188996697342645 1102 12 5.263812881751065 1102 13 9.943147625123832 1102 14 7.287373043400059 1102 15 5.269463518717316 1102 16 5.621679930941596 1102 17 6.450898839504912 1102 18 6.563185070816807 1102 19 5.359625721070554 1102 20 5.615233692566246 1102 21 4.583909653879708 1102 22 5.836027921871203 1102 23 6.223125464878233 1102 24 7.646111223518222 1102 25 7.247408211931219 1103 1 4.903903894567218 1103 2 9.411801841623344 1103 3 6.186493391490377 1103 4 7.688561752177428 1103 5 7.055633625308381 1103 6 6.83247367264762 1103 7 6.5750993063533905 1103 8 5.848378666087445 1103 9 5.186784263629605 1103 10 4.4074354343056115 1103 11 5.530694269764474 1103 12 4.179863401833902 1103 13 8.565227326538803 1103 14 6.598174703255651 1103 15 5.735240808302272 1103 16 5.524171289141272 1103 17 3.0050744629350135 1103 18 1.7381647950666022 1103 19 4.044619303697189 1103 20 4.446189054419484 1103 21 6.63714629353829 1103 22 2.1484713269373863 1103 23 4.563665547871015 1103 24 6.079707157344469 1103 25 1.6775946700923043 1104 1 5.80551800670527 1104 2 10.489721168604511 1104 3 7.104524370210367 1104 4 7.789731413186306 1104 5 7.460248346592653 1104 6 7.165980600119113 1104 7 7.06238306571306 1104 8 7.017053259175263 1104 9 5.758629675856145 1104 10 4.774354533170293 1104 11 7.799018791785237 1104 12 4.86743531492904 1104 13 9.153488351960007 1104 14 6.77272016235888 1104 15 6.063329342566121 1104 16 5.484525396115366 1104 17 5.878407771200603 1104 18 3.3289858640054106 1104 19 4.556842336443182 1104 20 3.700317085423272 1104 21 2.9713168895740765 1104 22 4.9105032181233135 1104 23 5.365298836834437 1104 24 6.28634497103597 1104 25 5.293937210808593 1105 1 3.435580451868475 1105 2 9.492870939642945 1105 3 6.170213634322508 1105 4 5.369101833879377 1105 5 1.8006636761636763 1105 6 4.435341956679363 1105 7 5.562804224386177 1105 8 3.0203203378699577 1105 9 3.5055885958033457 1105 10 2.9355195312262516 1105 11 6.3727681198360955 1105 12 3.6563305126317474 1105 13 4.4562219226901725 1105 14 3.4945655690082873 1105 15 3.940198630998438 1105 16 4.1858244272387815 1105 17 4.821216660702234 1105 18 2.4698197521313965 1105 19 2.2358420286617573 1105 20 1.5972580766487816 1105 21 2.5337986358376163 1105 22 3.871063658474487 1105 23 1.0853586274584117 1105 24 4.208791307318322 1105 25 4.9619807307397075 1106 1 6.26336105014088 1106 2 11.035186547532323 1106 3 7.527724975440407 1106 4 8.872458222481665 1106 5 8.442244317744318 1106 6 7.515802479762836 1106 7 7.278090759344842 1106 8 7.127755707600407 1106 9 6.036129093556955 1106 10 5.314044467128234 1106 11 7.948194514934624 1106 12 5.292011771263823 1106 13 4.556223924331519 1106 14 5.694300260546257 1106 15 5.687966345979266 1106 16 5.87624693733342 1106 17 6.110805032257819 1106 18 6.184745991647802 1106 19 5.890113076375428 1106 20 5.535276342535898 1106 21 5.776923452732923 1106 22 6.489672736099958 1106 23 6.495336627600345 1106 24 7.413510085807591 1106 25 6.422845912916365 1107 1 4.24393963071946 1107 2 -14.013764501418727 1107 3 -5.541544493829063 1107 4 2.3113766614001037 1107 5 5.032581308081308 1107 6 5.3449607089210645 1107 7 5.110556191810275 1107 8 5.725567505412208 1107 9 4.093655351083214 1107 10 3.3491955022792688 1107 11 6.181266548006658 1107 12 3.2624701417221935 1107 13 4.933163501271096 1107 14 4.2352116014576 1107 15 4.032512490525409 1107 16 3.740011401097888 1107 17 4.434318755771542 1107 18 -0.6944606875588804 1107 19 2.2974450837074336 1107 20 2.577079584339142 1107 21 -1.2424403666308947 1107 22 2.2777687241959477 1107 23 -1.2241672919035729 1107 24 2.713633385930894 1107 25 3.5264426165130693 1108 1 5.310389351406467 1108 2 10.703492817533508 1108 3 0.5803305619442973 1108 4 6.286402094052654 1108 5 7.445648634707958 1108 6 7.384673721515433 1108 7 7.472762377745273 1108 8 7.192839399802743 1108 9 5.859940874995857 1108 10 4.629888698226704 1108 11 7.1699887282542605 1108 12 2.833535219566933 1108 13 3.9253980646921 1108 14 4.9080215674879035 1108 15 4.998931275588262 1108 16 4.829475441409386 1108 17 -0.03177461032182549 1108 18 3.488935211091256 1108 19 4.740309960470615 1108 20 4.870743135629812 1108 21 4.954231079193093 1108 22 1.748381071079482 1108 23 4.530262154051297 1108 24 5.267348337950928 1108 25 5.867639349235223 1109 1 3.414820794721429 1109 2 -14.013764501418727 1109 3 -5.7976864699374815 1109 4 1.7646624015985957 1109 5 0.9023734876050327 1109 6 2.4782404891807097 1109 7 3.670177606129677 1109 8 4.088976719995916 1109 9 3.2802390104856656 1109 10 2.3917021748194998 1109 11 0.856260069812258 1109 12 1.3379806234407283 1109 13 2.720968948136946 1109 14 3.4414041074823203 1109 15 3.1769775212320503 1109 16 3.117582596454316 1109 17 3.6633138582565756 1109 18 3.636654727952507 1109 19 1.9570153202910916 1109 20 1.0999949013819759 1109 21 0.40666693818110744 1109 22 2.6205967151783014 1109 23 2.9643980471651226 1109 24 4.140264595280222 1109 25 4.500448213673028 1110 1 3.5604166396182144 1110 2 9.253945548004666 1110 3 6.655956985056271 1110 4 8.040584805764755 1110 5 8.43261629246555 1110 6 4.669687035624328 1110 7 7.245747301960197 1110 8 7.6102373482369075 1110 9 5.8471623765836505 1110 10 5.18266029208673 1110 11 3.514250461550702 1110 12 5.933354228916002 1110 13 10.378095536153708 1110 14 8.046333455248316 1110 15 7.1741000182743875 1110 16 6.8509201681845795 1110 17 7.140832848117594 1110 18 7.315182916977641 1110 19 6.042385387626318 1110 20 6.302999832664664 1110 21 4.570123479870347 1110 22 6.538822835497175 1110 23 7.038819229567295 1110 24 8.088844087335996 1110 25 8.522669391652531 1111 1 4.863044677712953 1111 2 9.366315179856173 1111 3 7.157060314735904 1111 4 7.679153430770501 1111 5 8.423960963205982 1111 6 7.581906362599785 1111 7 7.381540209407838 1111 8 7.571847251293544 1111 9 6.045701990779261 1111 10 5.288480832002847 1111 11 7.15830357364926 1111 12 6.316733397579075 1111 13 8.087262164533104 1111 14 6.2879889092149845 1111 15 5.748724091995975 1111 16 5.625011939484878 1111 17 6.668732452336631 1111 18 6.439957623751859 1111 19 5.416822400694311 1111 20 5.082255790710569 1111 21 7.799476906362074 1111 22 7.083029534237632 1111 23 6.837193442763933 1111 24 6.691885627928155 1111 25 7.522741590501283 1112 1 -2.4612800832593784 1112 2 7.576665344485573 1112 3 5.820710983754882 1112 4 0.09127752159293578 1112 5 4.094488261959064 1112 6 2.9394607355378817 1112 7 5.588278543985183 1112 8 5.787429367274068 1112 9 4.287060643758583 1112 10 3.2475286633861558 1112 11 -0.29518821749920576 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4.210830601998595 2204 7 5.090811319082107 2204 8 5.112542069475076 2204 9 3.241290678193483 2204 10 3.0972964842704656 2204 11 4.750417219305298 2204 12 4.9113129156246345 2204 13 2.838234409205967 2204 14 4.065580531587866 2204 15 4.286651935118314 2204 16 3.9628370907493533 2204 17 -0.6949336469892167 2204 18 2.7662077544938093 2204 19 3.4130995038964613 2204 20 3.7277926567705926 2204 21 5.289083793055553 2204 22 4.778303490124507 2204 23 3.8820437947371076 2204 24 5.256609706950172 2204 25 5.390354670401255 2205 1 5.800793291102529 2205 2 10.508588439757744 2205 3 7.158357088425461 2205 4 7.480615816521613 2205 5 7.651868157956393 2205 6 7.180006511025692 2205 7 7.429805188706329 2205 8 7.160501262698904 2205 9 5.826525363953227 2205 10 4.96911828220205 2205 11 7.515847515528801 2205 12 6.465646763722347 2205 13 9.90351401985691 2205 14 7.1658921980205506 2205 15 6.1273538865432755 2205 16 5.534394019009916 2205 17 6.253608633884948 2205 18 6.017968716635231 2205 19 4.940119018146074 2205 20 5.142960423161156 2205 21 -1.6308301009029833 2205 22 3.416044142471364 2205 23 4.742894559864162 2205 24 6.7801849795413105 2205 25 7.112464268417071 2206 1 4.2965941079703995 2206 2 8.874014130402948 2206 3 6.068284595169896 2206 4 1.2922882856629982 2206 5 5.746126872971992 2206 6 6.135963768640497 2206 7 6.37211442293038 2206 8 5.99692633541823 2206 9 4.896328079812825 2206 10 3.9269660915794518 2206 11 3.658378600214789 2206 12 4.817800612194862 2206 13 8.28661540491516 2206 14 0.4263066605003907 2206 15 2.7110852545708894 2206 16 3.6863977567079154 2206 17 4.654934913820444 2206 18 -0.8212126069318622 2206 19 1.4455444073640216 2206 20 2.808709545254267 2206 21 3.563523563698908 2206 22 -1.8146911893246873 2206 23 -2.8778960838337504 2206 24 1.4826141818140712 2206 25 3.14039090102246 2207 1 1.1217187812986076 2207 2 -14.013764501418727 2207 3 -6.592670362554932 2207 4 -4.600419477596034 2207 5 0.79064462854463 2207 6 1.3887908247511795 2207 7 2.621632639686723 2207 8 2.7729404783851805 2207 9 0.28919203061989407 2207 10 0.07871828220205046 2207 11 -2.0414701315300228 2207 12 -2.7988395107874595 2207 13 0.19811794142553651 2207 14 -6.148476429430431 2207 15 -2.151626505613585 2207 16 -0.9478804907940059 2207 17 0.19258902604181216 2207 18 0.20416479506660323 2207 19 -0.333418236755886 2207 20 -0.5570238905643343 2207 21 -3.8977948067853347 2207 22 -4.318889190861967 2207 23 -1.7127603420966224 2207 24 0.11856929326679833 2207 25 0.5855623076327596 2208 1 4.4258913303182155 2208 2 6.296944397525465 2208 3 6.082640648003139 2208 4 7.780977807471839 2208 5 3.295276906071024 2208 6 5.680549497451029 2208 7 6.394480905146754 2208 8 6.412869286831636 2208 9 4.827641502716425 2208 10 4.257323410407178 2208 11 6.475183865453385 2208 12 5.864409961309072 2208 13 8.482066056056002 2208 14 3.093313012500186 2208 15 4.397459467237095 2208 16 4.8651632497791475 2208 17 5.544257201004104 2208 18 0.30189028526267947 2208 19 2.798701220257687 2208 20 3.9077568032516545 2208 21 6.596590713576656 2208 22 5.433751533119931 2208 23 1.629712056093421 2208 24 3.2672739689832397 2208 25 4.5784974509208425 2209 1 2.661185447965277 2209 2 4.595070839946736 2209 3 5.056396304111736 2209 4 0.4840271087493484 2209 5 4.9128839859743465 2209 6 5.325851065715035 2209 7 5.930320189887524 2209 8 4.339504333806865 2209 9 4.0567261671660795 2209 10 2.98453996894904 2209 11 -1.0918444287187796 2209 12 2.760161292425394 2209 13 2.733960511706659 2209 14 3.640468148882821 2209 15 3.886172691173563 2209 16 3.419203846555394 2209 17 1.3696492670056664 2209 18 3.352020216753349 2209 19 3.960917506215999 2209 20 3.9069953865441 2209 21 6.238690333776912 2209 22 4.7868071946802 2209 23 0.875037248264821 2209 24 -2.475416248901876 2209 25 0.8071205405644868 2210 1 -2.213239839391047 2210 2 -14.013764501418727 2210 3 -7.793891052210103 2210 4 -2.374171201733965 2210 5 -0.7029898542139925 2210 6 -1.3745540028350263 2210 7 -1.2432914982443108 2210 8 -21.10145952161482 2210 9 -13.301807969380105 2210 10 -7.449329993660019 2210 11 -10.357752890150714 2210 12 -4.909867096994354 2210 13 -5.885302748229636 2210 14 -4.288779877706293 2210 15 -6.071385126303241 2210 16 -5.340673594242283 2210 17 -4.855755801544397 2210 18 -4.902352446312708 2210 19 -10.654052719514507 2210 20 -7.3762721664264035 2210 21 -5.617077565406024 2210 22 -10.264013328793002 2210 23 -8.339015514510418 2210 24 -10.977306568802165 2210 25 -8.453127347539654 2211 1 0.6929181073306232 2211 2 5.436951589566952 2211 3 1.1492915582538323 2211 4 2.0662305645269647 2211 5 1.6468946706676277 2211 6 0.5305465113560679 2211 7 -20.841567360313277 2211 8 -13.008788923468233 2211 9 -8.428661381343037 2211 10 -6.445439426306795 2211 11 -9.69490029159742 2211 12 -4.196442206659405 2211 13 -6.380346759501171 2211 14 -4.211346185117037 2211 15 -4.412994660626222 2211 16 -11.310936935612876 2211 17 -7.090861690049174 2211 18 -4.9621300659275 2211 19 -8.03548664450652 2211 20 -5.701193730496936 2211 21 -3.4093007882512154 2211 22 -4.993312779741498 2211 23 -6.957932372425184 2211 24 -22.3790307067332 2211 25 -22.18243769236724 2212 1 -1.251048594587914 2212 2 -14.013764501418727 2212 3 -8.067362561136491 2212 4 -19.827619477596034 2212 5 -20.24695537145537 2212 6 -21.13920917524882 2212 7 -10.153624097901929 2212 8 -9.756601365586452 2212 9 -5.678403714060956 2212 10 -5.954215051131282 2212 11 -5.692950982593853 2212 12 -3.1700040497945494 2212 13 -6.47898702311347 2212 14 -4.016953025175113 2212 15 -4.2008605481667765 2212 16 -4.339114533347196 2212 17 -4.322715938497197 2212 18 -3.523360027628435 2212 19 -4.080819655195604 2212 20 -4.312185592691993 2212 21 -2.141282750047747 2212 22 -3.325587063202395 2212 23 -3.864431264082441 2212 24 -2.579385316662279 2212 25 -3.0024731533601496 2213 1 -19.00548121870139 2213 2 -14.013764501418727 2213 3 -8.331760333901636 2213 4 -5.280341540633284 2213 5 -20.24695537145537 2213 6 -10.394223501896384 2213 7 -20.841567360313277 2213 8 -10.240905557908995 2213 9 -7.665776450755462 2213 10 -6.493613331933574 2213 11 -6.031603464863355 2213 12 -4.328148393308947 2213 13 -19.426682058574464 2213 14 -12.038656372123842 2213 15 -6.26677836807777 2213 16 -5.363723852780634 2213 17 -4.021485472525523 2213 18 -4.0166107541215545 2213 19 -4.982712601607844 2213 20 -5.091329143668439 2213 21 -3.130159276699377 2213 22 -4.037098168894442 2213 23 -3.806738565592326 2213 24 -2.9377699617475272 2213 25 -3.7784262310491883 2214 1 -19.00548121870139 2214 2 -14.013764501418727 2214 3 -12.074400797337539 2214 4 -19.827619477596034 2214 5 -20.24695537145537 2214 6 -21.13920917524882 2214 7 -16.293741273356755 2214 8 -14.603633434658299 2214 9 -13.302894925901844 2214 10 -13.312186065624037 2214 11 -14.035226653269154 2214 12 -11.721561249917892 2214 13 -19.426682058574464 2214 14 -16.57176338595217 2214 15 -24.163626505613585 2214 16 -18.027967447315746 2214 17 -23.89541097395819 2214 18 -17.562922161455138 2214 19 -14.484974758495017 2214 20 -13.046736934042594 2214 21 -10.429333937220118 2214 22 -11.568810929992402 2214 23 -11.468925559487928 2214 24 -10.270335054559288 2214 25 -10.00743769236724 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 118550.0 3 0.0 4 580.0 5 2102.0 6 3120.0 7 2921.0 8 2487.0 9 2326.0 10 2221.0 11 2127.0 12 2040.0 13 2199.0 14 3211.0 15 7125.0 16 10461.0 17 10872.0 18 11205.0 19 13127.0 20 15809.0 21 18344.0 22 19507.0 23 21142.0 24 22411.0 25 22981.0 26 18957.0 27 14975.0 28 16015.0 29 18696.0 30 17944.0 31 21391.0 32 27323.0 33 34288.0 34 41926.0 35 48137.0 36 47635.0 37 49053.0 38 37376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36159123099505 18.64603245290663 11.889217037052518 26.1031592790458 2 13.693970291934512 19.980985442524574 41.48706050641523 24.837983759125677 3 18.842813321126663 24.362716539645927 29.37924310699466 27.415227032232753 4 11.153446820347447 17.452590654302337 38.67334693969965 32.72061558565056 5 12.09385262104745 38.965620184122834 36.14068210974754 12.799845085082179 6 31.797524743582834 37.729452742655305 18.14181040887944 12.331212104882418 7 27.437305421945073 32.13798612583966 22.319718739405122 18.104989712810145 8 24.088591875586378 38.39117906926611 20.42459002954277 17.095639025604747 9 26.43821142792742 15.347402231488786 20.03254254479883 38.181843795784964 10 13.814036929079512 29.89250409277333 35.43671460107581 20.856744377071344 11 33.21491901501087 22.123906959045986 25.312323957792977 19.348850068150163 12 23.6027473547429 24.60917022461481 32.513978095414885 19.2741043252274 13 28.373739167082867 21.759250809305815 25.340273479824184 24.526736543787138 14 19.873265143487163 22.89724965081505 27.949934447488012 29.279550758209773 15 22.014097933334973 32.59405734355359 23.361400940537273 22.030443782574167 16 21.114792522715433 28.272469471612943 29.08660802129753 21.526129984374094 17 20.87057105470529 28.879795840171198 29.319057568155692 20.930575536967822 18 20.738657443080314 27.573871451972852 31.51030518883809 20.17716591610874 19 22.79536553990262 27.08440889811775 27.338265956627524 22.781959605352107 20 22.914311601105876 27.954963384463838 28.655802669850505 20.474922344579785 21 21.545000734106594 26.720745852297757 26.82557627367494 24.908677139920716 22 22.13197172034564 29.13275726630008 27.845318860244234 20.88995215311005 23 21.312588577047592 28.50215684230398 28.619649101057433 21.565605479591003 24 22.376321261813704 27.419660653258664 28.982129341453977 21.221888743473656 25 22.046740453541318 27.779969343131512 28.671920788061854 21.501369415265316 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 652.0 1 652.0 2 1397.0 3 2142.0 4 2142.0 5 2142.0 6 3102.5 7 4063.0 8 4063.0 9 4063.0 10 4773.5 11 5484.0 12 5484.0 13 5484.0 14 6803.0 15 8122.0 16 8122.0 17 8122.0 18 11693.5 19 15265.0 20 15265.0 21 15265.0 22 21502.0 23 27739.0 24 27739.0 25 27739.0 26 37045.0 27 46351.0 28 46351.0 29 46351.0 30 56518.0 31 66685.0 32 66685.0 33 66685.0 34 73784.5 35 80884.0 36 80884.0 37 80884.0 38 84093.5 39 87303.0 40 87303.0 41 87303.0 42 88172.0 43 89041.0 44 89041.0 45 89041.0 46 87540.0 47 86039.0 48 86039.0 49 86039.0 50 78471.5 51 70904.0 52 70904.0 53 70904.0 54 60823.5 55 50743.0 56 50743.0 57 50743.0 58 42016.5 59 33290.0 60 33290.0 61 33290.0 62 26379.5 63 19469.0 64 19469.0 65 19469.0 66 14720.5 67 9972.0 68 9972.0 69 9972.0 70 7066.5 71 4161.0 72 4161.0 73 4161.0 74 2858.0 75 1555.0 76 1555.0 77 1555.0 78 1039.5 79 524.0 80 524.0 81 524.0 82 336.0 83 148.0 84 148.0 85 148.0 86 94.0 87 40.0 88 40.0 89 40.0 90 22.5 91 5.0 92 5.0 93 5.0 94 4.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.465099129729913 2 35.462098780721206 3 5.309435619152697 4 12.981716447316574 5 12.790746766040328 6 13.820040980376705 7 9.096039314141608 8 7.300755434966169 9 0.10470261081026312 10 1.315537642277338 11 5.320271776454296 12 5.236397104353602 13 15.18567825900949 14 1.461051754613107 15 2.712839016921293 16 2.733526226315256 17 2.6694943877148933 18 1.3752068720939397 19 1.3231370253200183 20 0.2821622777884107 21 4.149263141303491 22 1.468369679024577 23 0.10554698670389427 24 8.706500568546435 25 0.11145761795931235 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 710584.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.56209046277857 #Duplication Level Percentage of deduplicated Percentage of total 1 97.00042171484114 89.78561809696797 2 2.1722143766103996 4.0212940726471995 3 0.37257161789007665 1.0345802339701513 4 0.1461509545733815 0.5411215151377112 5 0.0840971537142711 0.3892104174881278 6 0.05207896305970637 0.2892322613964147 7 0.02547899056618366 0.16508720407811617 8 0.02358136313603206 0.17461922142664252 9 0.015629393716066503 0.13020204195224375 >10 0.09388973408430314 1.5912377991791298 >50 0.006865042707732112 0.44466079959446636 >100 0.006396633317880236 1.015932500775746 >500 6.24061782752331E-4 0.41720383538604916 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 924 0.13003388761919774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.4072931560519234E-4 0.0 11 0.0 0.0 0.0 1.4072931560519234E-4 0.0 12 0.0 0.0 0.0 1.4072931560519234E-4 0.0 13 0.0 0.0 0.0 1.4072931560519234E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAG 45 0.0012399488 17.701933 1 GCTCTAG 70 4.6231635E-6 17.069721 1 TTATATA 90 1.3035424E-7 16.620222 2 GTCCTAA 320 0.0 16.180672 1 ATAGGAC 115 1.8189894E-12 16.104488 3 GTCATAG 50 0.0025088775 15.931739 1 TAGGACC 270 0.0 15.43017 4 TGTAGGA 625 0.0 15.012593 2 GTCCTAG 80 1.6416741E-5 14.936005 1 CTAAGGT 65 1.8019155E-5 14.791051 4 CTGTAGG 610 0.0 14.582329 1 TATAGGA 75 1.294452E-4 14.504921 2 GTATTGA 55 0.004731615 14.483398 1 GTATTGG 65 8.665037E-4 14.297714 1 CTAAGGC 70 3.0124285E-5 14.0068445 3 TCTAAGG 70 3.0124285E-5 14.0068445 3 TAGGACA 210 0.0 13.734546 4 CTTAGAC 80 6.9885227E-6 13.617765 3 TACTCTC 45 0.0074162786 13.534925 7 CTGCTAG 70 0.0015163792 13.276449 1 >>END_MODULE