FastQCFastQC Report
Thu 26 May 2016
SRR1926495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710584
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18930.26640059444062913No Hit
GTCCTACAGTGGACATTTCTAAATT16700.2350179570606712No Hit
CTTTAGGACGTGAAATATGGCGAGG14340.20180583857784584No Hit
GTCCTAAAGTGTGTATTTCTCATTT14310.20138365063103025No Hit
GTCCTACAGTGTGCATTTCTCATTT13850.1949101021131914No Hit
CTGTAGGACCTGGAATATGGCGAGA10870.1529727660628441No Hit
CCCATGTACTCTGCGTTGATACCAC10270.14452900712653255No Hit
GTATCAACGCAGAGTACTTTTTTTT10210.1436846312329014No Hit
GTACATGGGAAGCAGTGGTATCAAC10180.1432624432860858No Hit
CTGAAGGACCTGGAATATGGCGAGA7500.10554698670389427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3850.013.8733144
TGTAGGA9800.013.3373792
GTAGGAC9800.012.6550093
AGGACCT6050.012.6121035
CTGTAGG10100.012.1786231
GGACCTG6300.012.1116236
CTACACT1800.011.6574374
TGTAGAA2250.011.4487292
GTATTAA1001.8407318E-611.4422411
CTTACAC855.0908435E-511.2234483
AGGACGT8050.011.1374275
AAAGGTC951.2985702E-511.0438885
GGACGTG8050.011.0189446
GGACTGA1303.06718E-811.0052726
GGCGAGG3450.010.98373419
GTGTAAC700.001444392310.8973721
GTCTTAC700.001444392310.8973721
CCGTTTC700.001515903710.83444813
GTCCTAC8450.010.83289051
GACGTGG4750.010.8010037
Produced by FastQC (version 0.11.5)