##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926495_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 710584 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.38779229478851 31.0 28.0 31.0 22.0 34.0 2 28.862193632279926 31.0 28.0 33.0 23.0 34.0 3 29.219814969095843 31.0 30.0 33.0 25.0 34.0 4 33.00875617801695 35.0 33.0 37.0 27.0 37.0 5 32.71987970458102 35.0 33.0 37.0 26.0 37.0 6 32.81784982493273 35.0 33.0 37.0 26.0 37.0 7 32.66153895950373 35.0 33.0 37.0 25.0 37.0 8 32.62798486878398 35.0 33.0 37.0 25.0 37.0 9 33.868568670276844 38.0 34.0 39.0 24.0 39.0 10 33.76335380475778 37.0 34.0 39.0 23.0 39.0 11 34.0220565056348 38.0 34.0 39.0 25.0 39.0 12 33.83293460027245 37.0 34.0 39.0 25.0 39.0 13 33.96581262736003 38.0 34.0 39.0 25.0 39.0 14 34.62713345642457 39.0 34.0 40.0 23.0 41.0 15 34.753868648885984 39.0 34.0 40.0 25.0 41.0 16 34.745688053769854 39.0 34.0 40.0 25.0 41.0 17 34.660002195377324 39.0 34.0 40.0 24.0 41.0 18 34.63590792925256 39.0 34.0 40.0 24.0 41.0 19 34.664563232496086 39.0 34.0 40.0 23.0 41.0 20 34.64727463607399 39.0 34.0 41.0 22.0 41.0 21 34.58000180133524 39.0 34.0 41.0 22.0 41.0 22 34.507209562838455 39.0 34.0 40.0 19.0 41.0 23 34.16081420352837 39.0 34.0 40.0 18.0 41.0 24 34.24471420690587 39.0 34.0 41.0 18.0 41.0 25 34.03775626808372 39.0 34.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2454338972345447 1101 2 1.064341590645217 1101 3 0.9701713455874206 1101 4 1.0877561374598628 1101 5 1.0948289301677434 1101 6 0.9088906427909365 1101 7 1.114580161247332 1101 8 1.2424702448437372 1101 9 1.409755670620612 1101 10 1.41539766664566 1101 11 1.181925017824284 1101 12 1.3589471396684658 1101 13 1.2940743657508236 1101 14 1.6923201454154082 1101 15 1.3286393530417584 1101 16 1.4105291544677172 1101 17 1.329210435730893 1101 18 1.5857102832840084 1101 19 1.5135506019923852 1101 20 1.5385464349516198 1101 21 1.462158574052033 1101 22 1.4176366518316144 1101 23 1.6014451387101687 1101 24 1.5305045060109421 1101 25 1.5820400300402184 1102 1 0.5976710635550084 1102 2 0.7196905849808992 1102 3 0.5147973369716787 1102 4 0.16437977698603845 1102 5 0.4646734419186487 1102 6 0.15384684845989938 1102 7 0.41625495755501873 1102 8 0.5665212172416361 1102 9 0.7224219221018089 1102 10 1.047555586695374 1102 11 0.5665634131969028 1102 12 0.6367281409359435 1102 13 0.6061984262647542 1102 14 0.9069703903361557 1102 15 0.7902978771734439 1102 16 0.7879159874148769 1102 17 0.8496654119608849 1102 18 0.961126274629585 1102 19 0.708756757052555 1102 20 0.5739474435170777 1102 21 0.7267097288994506 1102 22 0.8190094716243621 1102 23 1.0201706482471877 1102 24 1.1029124657095792 1102 25 0.7749226937257987 1103 1 0.1747149772866159 1103 2 0.10926058159504848 1103 3 0.0019035346582754187 1103 4 -0.11578778338845552 1103 5 -0.07366937967837117 1103 6 -0.3443558932939723 1103 7 -0.2836324431331718 1103 8 -0.1570376296784204 1103 9 0.31436359093384425 1103 10 0.22791921160580841 1103 11 -0.09002640247094007 1103 12 0.022542202463988303 1103 13 0.05642638081557294 1103 14 0.5005597099371002 1103 15 0.3224241143879141 1103 16 0.22709857940378697 1103 17 0.35438395305175874 1103 18 0.21164990079986978 1103 19 0.36912219300086946 1103 20 0.2646398899142923 1103 21 0.13158299561808917 1103 22 0.27565086250145043 1103 23 0.4756958886034113 1103 24 0.33623713755739004 1103 25 0.249034694607694 1104 1 0.5855678070590749 1104 2 0.6175717926429023 1104 3 0.4393181476498853 1104 4 0.21430178366651376 1104 5 0.38676995763071176 1104 6 0.07660487819096318 1104 7 0.0032603847341334813 1104 8 0.024954829943709456 1104 9 0.5564302761506426 1104 10 0.4247586702427739 1104 11 0.18943697229937584 1104 12 0.22083308357017728 1104 13 0.05209091421709644 1104 14 0.4983029861410415 1104 15 0.4611126702100208 1104 16 0.6286393683860112 1104 17 0.6679620227852112 1104 18 0.5853291439544961 1104 19 0.5644958152149044 1104 20 0.5794625732159133 1104 21 0.6607543311523685 1104 22 0.6823474600563415 1104 23 0.8269595536582202 1104 24 0.7560441660519004 1104 25 0.868168528794584 1105 1 0.7956573539189478 1105 2 0.6284620549585433 1105 3 0.8504820875182233 1105 4 0.6533776895809353 1105 5 0.8914746597932179 1105 6 0.41110100855000553 1105 7 0.4919979427152583 1105 8 0.4071927576858201 1105 9 0.9583613778569884 1105 10 1.1629896586928083 1105 11 0.7471097093349144 1105 12 1.016009078311889 1105 13 0.7105762788776175 1105 14 1.288564757571308 1105 15 1.1254489053961478 1105 16 1.039496736183608 1105 17 0.804633303149906 1105 18 0.8411850030788841 1105 19 0.8230322147526863 1105 20 0.8863079587015719 1105 21 0.9529187239250092 1105 22 1.2565896011992237 1105 23 1.4116360294217927 1105 24 1.1241682896457945 1105 25 1.4494261515246976 1106 1 0.36773554894796234 1106 2 0.418333385813483 1106 3 0.4170029983014274 1106 4 0.30954847689926623 1106 5 0.11498339149867576 1106 6 -1.1554108038634467 1106 7 -0.8689780576050019 1106 8 -0.7689389430360407 1106 9 -0.49862062010861763 1106 10 -0.5315198390953739 1106 11 -0.5863582798707796 1106 12 -0.6487487503803635 1106 13 -0.7879961869079608 1106 14 -0.5831809830268924 1106 15 -0.7087351255091932 1106 16 -0.7117465560432876 1106 17 -0.7742832265862987 1106 18 -0.7485476686210006 1106 19 -0.8213052328634447 1106 20 -0.7076954877608941 1106 21 -0.621681539199848 1106 22 -0.7137351560107774 1106 23 -0.5085585301170283 1106 24 -0.6593196249896636 1106 25 -0.7868148388146494 1107 1 0.9186566998691141 1107 2 0.9622975297776257 1107 3 0.8058969871954176 1107 4 0.8501454174962007 1107 5 0.92869220520749 1107 6 0.6531332046805574 1107 7 0.9519637633368205 1107 8 0.8818979078008056 1107 9 1.1725310510430518 1107 10 0.8484887409132043 1107 11 1.0083161148036126 1107 12 1.1460468444152312 1107 13 1.00127400236223 1107 14 1.197044197198288 1107 15 1.0777876610135948 1107 16 1.0796638353671 1107 17 1.0928034405003686 1107 18 1.3001549800816505 1107 19 1.3548633433051265 1107 20 0.8710242909588857 1107 21 1.0657697482514408 1107 22 1.1099600676844403 1107 23 1.306350884792387 1107 24 1.3410427753727419 1107 25 1.1314608794610663 1108 1 1.2971338342784513 1108 2 1.0021902069584385 1108 3 0.8553499620721183 1108 4 0.8383238446576797 1108 5 -0.5487991536398056 1108 6 0.45646413851996925 1108 7 0.6725585602028019 1108 8 0.861663035023561 1108 9 1.1611979041166833 1108 10 0.9559823009491382 1108 11 0.9560277709898486 1108 12 0.9336725947528492 1108 13 0.7803930509727977 1108 14 1.266232315538943 1108 15 1.2602572756865982 1108 16 1.1651449140685202 1108 17 1.0250859541388166 1108 18 1.0810718643883632 1108 19 1.2009907385850696 1108 20 1.2776534382660003 1108 21 1.1192413796891714 1108 22 1.337645666556483 1108 23 1.5283621762952038 1108 24 1.33231773952906 1108 25 1.371778921474025 1109 1 0.5917210153830936 1109 2 -0.24223591066185435 1109 3 0.5428751686366837 1109 4 0.699550719250496 1109 5 -0.7212715169038866 1109 6 0.14208010939927362 1109 7 0.5075563885603174 1109 8 0.6221890378570407 1109 9 1.1465729260916433 1109 10 -1.312932963629308 1109 11 0.1220291567045777 1109 12 0.7864727209149365 1109 13 0.7192432179824522 1109 14 1.0815418780046926 1109 15 0.8671912784373461 1109 16 0.8181125669366409 1109 17 0.8546663100813632 1109 18 0.9397237914625407 1109 19 0.9795400814046857 1109 20 0.8232398334428837 1109 21 0.9120123955008026 1109 22 0.6865264950898009 1109 23 1.0189969680760527 1109 24 0.8565586987745704 1109 25 1.0209798087450963 1110 1 0.6047278263026783 1110 2 -0.4883439566134484 1110 3 0.07267708807765771 1110 4 0.5695019592580195 1110 5 0.6429938214860371 1110 6 0.487123396331377 1110 7 0.5607238531512735 1110 8 0.7625827112203751 1110 9 0.7221039235154336 1110 10 0.5188188026765417 1110 11 0.30114749643236394 1110 12 0.4564817321153569 1110 13 0.4963265743505474 1110 14 0.8306794563689621 1110 15 0.7764448967383757 1110 16 0.396163107055834 1110 17 0.7824467906050074 1110 18 0.6864703611252025 1110 19 0.7985365764347065 1110 20 0.6760283300650656 1110 21 0.6143967701898276 1110 22 0.697711716721102 1110 23 1.0677942768782813 1110 24 0.7930836371335346 1110 25 0.9097260356504364 1111 1 -0.20817804608913804 1111 2 -0.07149471437318411 1111 3 0.14172741067524086 1111 4 0.012104530849740058 1111 5 0.267165339696561 1111 6 0.02765057919793179 1111 7 0.3298437491849313 1111 8 0.23637190370907035 1111 9 0.5312075419904616 1111 10 0.4705164601520764 1111 11 0.009328282349649442 1111 12 0.20301126472626407 1111 13 0.1550957808851976 1111 14 0.36377803405164144 1111 15 0.3986847675680707 1111 16 0.3882488005257727 1111 17 0.08666582878507256 1111 18 0.16915773354655528 1111 19 0.11211849259215256 1111 20 -0.06448310032539695 1111 21 0.18071075641954337 1111 22 0.0761124465858245 1111 23 0.4649627693006906 1111 24 0.38300927231931325 1111 25 0.5042861805732173 1112 1 0.0275169678534688 1112 2 -0.008614362302150624 1112 3 0.2860868746845746 1112 4 0.4758153001003933 1112 5 0.2286637095585533 1112 6 0.2552255994736825 1112 7 0.46167263800919756 1112 8 0.5129932367063574 1112 9 0.3615135684927253 1112 10 0.6323082225429033 1112 11 0.2072771196040364 1112 12 0.22796901684835547 1112 13 0.03442227054699032 1112 14 0.5768811770352684 1112 15 0.5015711942861785 1112 16 0.5892136354278534 1112 17 0.41252023758943324 1112 18 0.3755626087739472 1112 19 0.29756454294063417 1112 20 0.107605188853654 1112 21 0.2417137840494945 1112 22 0.17910679522533002 1112 23 0.5238202832836834 1112 24 0.23754344705589148 1112 25 0.47565262073310066 1113 1 -0.07979203457962214 1113 2 -0.00413747665738029 1113 3 -1.4552269939285658 1113 4 -0.9642106528598653 1113 5 -1.379680167164885 1113 6 -0.7607938652465123 1113 7 -0.1560207926477375 1113 8 -0.2481681582652584 1113 9 0.0903894809014858 1113 10 0.22581349423795416 1113 11 -0.06488508639758805 1113 12 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0.44007406820790607 2202 19 0.3878157037475276 2202 20 0.37774797537181115 2202 21 0.39983426239563613 2202 22 0.6020088609284571 2202 23 0.6625723310536742 2202 24 0.4945630571798745 2202 25 0.4899435510911445 2203 1 0.03319829133378249 2203 2 0.17941845459085926 2203 3 0.15915827891824819 2203 4 -0.07840544951620387 2203 5 0.08557690055371836 2203 6 -0.04538407845210912 2203 7 0.268337618172211 2203 8 0.4171655507607568 2203 9 0.748346088396552 2203 10 0.7842498612897089 2203 11 0.34488142059969107 2203 12 0.5360493574946688 2203 13 0.5667260139680863 2203 14 0.8529213761523877 2203 15 0.8930306301027144 2203 16 0.7401605112483978 2203 17 0.763836773072164 2203 18 0.8305655258768141 2203 19 0.6919678188711416 2203 20 0.6154258353604334 2203 21 0.8455691588200835 2203 22 0.7918361957144171 2203 23 0.9403003033571906 2203 24 0.949217137393255 2203 25 0.988187936188119 2204 1 0.63013742710163 2204 2 0.7969126426743607 2204 3 0.6027098928388703 2204 4 0.3089328104124718 2204 5 0.45342935285633246 2204 6 0.3859640239075617 2204 7 0.4651916178534563 2204 8 0.5255109719389353 2204 9 1.0836941746166815 2204 10 0.8848153505095055 2204 11 0.6275339006085119 2204 12 0.862606772908336 2204 13 0.7453026542667729 2204 14 1.3257541550251872 2204 15 1.1734834249671167 2204 16 0.9204835845400723 2204 17 0.8080241504704802 2204 18 0.896828434988997 2204 19 1.068894728935561 2204 20 1.1026402915486315 2204 21 1.045235473779222 2204 22 1.1469888455246746 2204 23 1.3719873361090293 2204 24 1.1173232495052403 2204 25 1.0311036521348669 2205 1 0.8142733248975027 2205 2 1.0335095821661504 2205 3 0.75784490149627 2205 4 0.7588743144603924 2205 5 0.7811564341423036 2205 6 0.8605917556685228 2205 7 0.47284195245030247 2205 8 0.7849821685321317 2205 9 1.2314090887446199 2205 10 1.1518788537150826 2205 11 1.108814260007641 2205 12 1.1356509022545822 2205 13 1.0977627600274573 2205 14 1.2673386933751374 2205 15 1.1972880016904028 2205 16 1.0425164358667658 2205 17 1.1537043414012729 2205 18 1.19960535482614 2205 19 1.3079438187385435 2205 20 1.3674706532876044 2205 21 1.3227309110949577 2205 22 1.3254585914182613 2205 23 1.6322559495209816 2205 24 1.4922851101444863 2205 25 1.527106272753521 2206 1 0.3118362855160335 2206 2 0.7636514036910711 2206 3 0.6916617241515439 2206 4 0.6290164374125737 2206 5 0.8014304315198899 2206 6 0.6434845530641908 2206 7 0.8310291689202884 2206 8 0.9937121047802648 2206 9 1.167595732502818 2206 10 1.0499132465652252 2206 11 0.9316515947878301 2206 12 0.9582501095008951 2206 13 0.8618374537020017 2206 14 1.2528569221885633 2206 15 1.127511811429521 2206 16 1.1533831899473341 2206 17 0.9622926068381119 2206 18 0.8737600774377086 2206 19 0.9502105517192092 2206 20 1.165112889275001 2206 21 1.0202757860780025 2206 22 1.1077093487542484 2206 23 1.3942381351700277 2206 24 1.471211727678508 2206 25 1.4234406158681665 2207 1 -0.3805031456907315 2207 2 0.2313291900092871 2207 3 0.34681745051587853 2207 4 0.596678236029021 2207 5 0.5372976106128959 2207 6 0.5572505791979339 2207 7 0.6631792073522647 2207 8 0.7042762861791871 2207 9 0.9706561475681497 2207 10 0.7173690497935112 2207 11 0.8531593580468595 2207 12 0.7243881571565467 2207 13 0.581715701203926 2207 14 -4.607029934075847 2207 15 -1.6147590571331278 2207 16 -0.2757894464861792 2207 17 -0.13002899193206474 2207 18 0.20779359012026077 2207 19 0.38358303442482367 2207 20 0.5213686925032874 2207 21 0.7640956169773077 2207 22 0.6547291796535575 2207 23 1.0979030083445096 2207 24 0.6737439336738973 2207 25 0.677867057067246 2208 1 0.42045733696386733 2208 2 0.7217750421963132 2208 3 0.6444814022504204 2208 4 0.6350432435705002 2208 5 0.8378683496777484 2208 6 0.743487683270331 2208 7 0.9668691017715716 2208 8 0.9368977039770741 2208 9 1.310534277281576 2208 10 1.1876857918749621 2208 11 0.9836860548794348 2208 12 0.9918198313646869 2208 13 0.5955908143260942 2208 14 1.2425423132846376 2208 15 1.297046674390252 2208 16 1.299017190014574 2208 17 1.0983800578416947 2208 18 1.1730895177220688 2208 19 0.8999800178335704 2208 20 1.08192012538413 2208 21 1.3444503681085322 2208 22 1.3594857407395295 2208 23 1.6302046674696982 2208 24 1.5252866184401128 2208 25 1.4537126075951434 2209 1 0.03534585029320425 2209 2 -0.03449892244051256 2209 3 0.6459242778251166 2209 4 0.9160677942619557 2209 5 0.5887160845084765 2209 6 0.7529517840172133 2209 7 0.7614410547418231 2209 8 0.8707180532474581 2209 9 0.7855220110219676 2209 10 0.511585917263389 2209 11 0.737503133147257 2209 12 0.7696315306505213 2209 13 0.5948948176697897 2209 14 0.8697861301811827 2209 15 0.7781148384492056 2209 16 0.8757222001001637 2209 17 0.9398890803570978 2209 18 0.8909164816865243 2209 19 1.0178705846256264 2209 20 0.924777527844654 2209 21 0.9722466209933742 2209 22 1.0588769708182113 2209 23 1.213838751316402 2209 24 1.2744443352803216 2209 25 1.322721675541139 2210 1 -0.5165583181045257 2210 2 -0.45850529274933294 2210 3 0.35714848499863905 2210 4 0.3841954774083334 2210 5 0.8642700244059967 2210 6 0.7595471309220727 2210 7 0.758910241835018 2210 8 0.6447590447998763 2210 9 1.1207802854991868 2210 10 0.9944380153107559 2210 11 0.6912145304606554 2210 12 0.5777536743979255 2210 13 0.7233915632728909 2210 14 1.0623217900620858 2210 15 0.8311926670048067 2210 16 0.8728105535138226 2210 17 0.8043365253093171 2210 18 1.2420901418444004 2210 19 1.2615002758041314 2210 20 1.082913520089491 2210 21 1.0767369962876572 2210 22 0.993480903791486 2210 23 0.9126616290341687 2210 24 1.0045163474670034 2210 25 0.9332049881017284 2211 1 -1.0665830108971335 2211 2 0.0169689541204896 2211 3 0.5356032971881639 2211 4 0.5632022461385446 2211 5 1.0375516965438152 2211 6 0.8594955665610371 2211 7 0.981488895642066 2211 8 0.9371086366425416 2211 9 1.345720006034874 2211 10 1.2495341719502093 2211 11 0.9218205206416386 2211 12 1.0987207603578923 2211 13 0.8431654063429335 2211 14 1.2458880103217922 2211 15 0.940938331240929 2211 16 1.2234213370016036 2211 17 1.1318116146391262 2211 18 1.1774218967756909 2211 19 1.2362104986202738 2211 20 1.1558037388385856 2211 21 1.1612858444415082 2211 22 1.300951926073104 2211 23 1.5968785770050005 2211 24 1.4545250625702764 2211 25 1.6044335271599124 2212 1 1.0277067833872806 2212 2 0.047883799938365 2212 3 0.47648695406197916 2212 4 0.2583945480857608 2212 5 0.5238139226696319 2212 6 0.5219768203326893 2212 7 0.5076813350118385 2212 8 0.4416592649025901 2212 9 0.5013256511142501 2212 10 0.4752768512119516 2212 11 0.43365155662841914 2212 12 0.6630434763054822 2212 13 0.35454832531740266 2212 14 0.42853602337095964 2212 15 0.3933288861292894 2212 16 -0.2165511486138385 2212 17 0.46391710735871783 2212 18 0.4525694766450812 2212 19 0.5160184954177254 2212 20 0.5115757847018685 2212 21 0.6683608652042636 2212 22 0.49781570447625256 2212 23 0.8322079728835163 2212 24 0.6954432244540385 2212 25 0.0026840783438402127 2213 1 0.3731923652930327 2213 2 0.2721949015661167 2213 3 -0.5156918140495463 2213 4 -1.2111689463969562 2213 5 -1.8884429815552046 2213 6 -0.6052051992165843 2213 7 -0.31462251184544243 2213 8 -0.06885456386856958 2213 9 -0.5895875964624153 2213 10 -0.5203768909896809 2213 11 -0.566396706900612 2213 12 -0.6839052525855749 2213 13 -0.504468826016705 2213 14 -7.657439103130287 2213 15 -3.3490505566555697 2213 16 -2.055866619361062 2213 17 -1.432656690117355 2213 18 -5.721898100424152 2213 19 -3.367770547237072 2213 20 -5.4309652138959486 2213 21 -2.4005569140637633 2213 22 -1.798319148904227 2213 23 -4.349275597195124 2213 24 -2.1130724942153094 2213 25 -1.554734280086528 2214 1 0.5024446803962235 2214 2 0.37361614653102393 2214 3 -1.414478201658035 2214 4 -1.8215391552753246 2214 5 -0.4115545633001503 2214 6 -0.5625668121064109 2214 7 -0.43119470569121887 2214 8 -0.41037588773385636 2214 9 -5.141813417649242 2214 10 -1.531761384989096 2214 11 -0.932136294127055 2214 12 -1.4031683645825836 2214 13 -1.3520582118395552 2214 14 -2.698360368858456 2214 15 -2.2099242745244325 2214 16 -1.322841620399224 2214 17 -1.6309333397581511 2214 18 -1.2561107577058266 2214 19 -4.334451748183874 2214 20 -2.2643878292358437 2214 21 -4.10246090476182 2214 22 -6.653070820346443 2214 23 -6.96792307861201 2214 24 -6.437612588065232 2214 25 -4.406150334237104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23527.0 3 14.0 4 425.0 5 2701.0 6 3236.0 7 2707.0 8 1978.0 9 1679.0 10 1489.0 11 1293.0 12 1275.0 13 1243.0 14 1291.0 15 1275.0 16 1358.0 17 1430.0 18 1668.0 19 1923.0 20 2489.0 21 3186.0 22 4106.0 23 5116.0 24 5927.0 25 7082.0 26 8196.0 27 9196.0 28 10621.0 29 12957.0 30 15762.0 31 19983.0 32 25626.0 33 33560.0 34 44509.0 35 58945.0 36 80036.0 37 119320.0 38 193455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.18662856128717 19.397544372155654 9.115852842620932 30.299974223936243 2 13.487473745262884 21.366428848958538 39.784021352914344 25.36207605286423 3 18.548107812856344 24.73400136835496 28.92311027645838 27.794780542330315 4 12.009431638219251 17.482597219778288 37.78567657927151 32.72229456273095 5 12.805494322964615 38.90606216726773 35.509694456409434 12.77874905335822 6 31.998666321944796 38.25452058936138 17.489831411226348 12.256981677467481 7 28.199282335441207 31.62419192239635 21.96575231408047 18.210773428081975 8 24.734858259688725 39.00336882657947 19.702911517889753 16.558861395842058 9 26.376784616339215 15.474337061919346 19.701854139520194 38.44702418222125 10 14.47516393869488 30.144451841644866 34.28855743801013 21.091826781650123 11 34.39234080760403 22.544685893457515 24.72391921426571 18.33905408467275 12 23.703464979375624 24.93093543606725 31.914935537394783 19.450664047162334 13 29.405812683651757 22.4440741699785 25.331136079619014 22.81897706675073 14 20.28282355989826 23.188658890732256 27.307078053218486 29.221439496150996 15 22.354744192723068 32.57085117102512 22.767509084205166 22.306895552046655 16 21.433426733449394 28.563688230318775 28.715680383020224 21.287204653211607 17 21.065512631060447 29.619449722046305 28.127929069031033 21.18710857786222 18 20.981145777186068 27.996205793483142 30.512823038827285 20.509825390503504 19 23.37464764153798 27.23479352462604 27.20749146106852 22.18306737276746 20 23.29708095145615 28.2022702772688 27.959930985372388 20.540717785902665 21 22.0864809790257 27.12740506400369 27.162868851536203 23.623245105434403 22 22.768877430395282 29.204147574389516 26.84763518458057 21.179339810634634 23 21.514697769721806 28.839517917656465 27.767582720691713 21.87820159193002 24 23.042660804805077 27.345190280643187 28.591914796603348 21.020234117948387 25 22.129262690969682 28.195962757393918 28.013999752316405 21.660774799319995 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 129.0 1 129.0 2 145.0 3 161.0 4 161.0 5 161.0 6 284.0 7 407.0 8 407.0 9 407.0 10 713.5 11 1020.0 12 1020.0 13 1020.0 14 1756.5 15 2493.0 16 2493.0 17 2493.0 18 4685.0 19 6877.0 20 6877.0 21 6877.0 22 11454.0 23 16031.0 24 16031.0 25 16031.0 26 24041.0 27 32051.0 28 32051.0 29 32051.0 30 43665.0 31 55279.0 32 55279.0 33 55279.0 34 63805.5 35 72332.0 36 72332.0 37 72332.0 38 78282.0 39 84232.0 40 84232.0 41 84232.0 42 88494.5 43 92757.0 44 92757.0 45 92757.0 46 95782.5 47 98808.0 48 98808.0 49 98808.0 50 92970.0 51 87132.0 52 87132.0 53 87132.0 54 75556.0 55 63980.0 56 63980.0 57 63980.0 58 54224.5 59 44469.0 60 44469.0 61 44469.0 62 36092.5 63 27716.0 64 27716.0 65 27716.0 66 21270.5 67 14825.0 68 14825.0 69 14825.0 70 10545.5 71 6266.0 72 6266.0 73 6266.0 74 4341.5 75 2417.0 76 2417.0 77 2417.0 78 1642.0 79 867.0 80 867.0 81 867.0 82 558.5 83 250.0 84 250.0 85 250.0 86 160.0 87 70.0 88 70.0 89 70.0 90 41.5 91 13.0 92 13.0 93 13.0 94 7.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08767436362203483 2 0.033212118482825397 3 0.034056494376456546 4 0.029412426961485202 5 0.02504981817772424 6 0.3893980162795672 7 0.18843655359535255 8 0.20194656789345103 9 0.20659063530842234 10 0.014776578138545195 11 0.0018294811028675005 12 0.00239239836528827 13 0.0 14 0.07458653727075194 15 0.0014072931560519236 16 0.0029553156277090393 17 0.00478479673057654 18 0.0037996915213401932 19 0.002110939734077885 20 0.0014072931560519236 21 0.0 22 0.0 23 0.0 24 2.814586312103847E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 710584.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.76675412928013 #Duplication Level Percentage of deduplicated Percentage of total 1 92.45347899574814 76.52074364437651 2 5.6250904242717334 9.311409522013331 3 0.9346008102253146 2.3206162640683385 4 0.3221999340962523 1.0666977090325909 5 0.17849417767211012 0.7386691858447791 6 0.10663515907661669 0.5295507595698605 7 0.061147239740114046 0.35426709900779163 8 0.0524017427017165 0.3469697723311015 9 0.03663036809912898 0.2728599003113071 >10 0.19570088227076377 3.013242843029011 >50 0.016383701969478547 0.9756269048699094 >100 0.0150179964759532 2.523695110560006 >500 6.826362008433818E-4 0.335466271657516 >1k 0.0015359314518976089 1.6901850133279315 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGA 1893 0.26640059444062913 No Hit GTCCTACAGTGGACATTTCTAAATT 1670 0.2350179570606712 No Hit CTTTAGGACGTGAAATATGGCGAGG 1434 0.20180583857784584 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1431 0.20138365063103025 No Hit GTCCTACAGTGTGCATTTCTCATTT 1385 0.1949101021131914 No Hit CTGTAGGACCTGGAATATGGCGAGA 1087 0.1529727660628441 No Hit CCCATGTACTCTGCGTTGATACCAC 1027 0.14452900712653255 No Hit GTATCAACGCAGAGTACTTTTTTTT 1021 0.1436846312329014 No Hit GTACATGGGAAGCAGTGGTATCAAC 1018 0.1432624432860858 No Hit CTGAAGGACCTGGAATATGGCGAGA 750 0.10554698670389427 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.4072931560519234E-4 0.0 11 0.0 0.0 0.0 1.4072931560519234E-4 0.0 12 0.0 0.0 0.0 1.4072931560519234E-4 0.0 13 0.0 0.0 0.0 1.4072931560519234E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 385 0.0 13.873314 4 TGTAGGA 980 0.0 13.337379 2 GTAGGAC 980 0.0 12.655009 3 AGGACCT 605 0.0 12.612103 5 CTGTAGG 1010 0.0 12.178623 1 GGACCTG 630 0.0 12.111623 6 CTACACT 180 0.0 11.657437 4 TGTAGAA 225 0.0 11.448729 2 GTATTAA 100 1.8407318E-6 11.442241 1 CTTACAC 85 5.0908435E-5 11.223448 3 AGGACGT 805 0.0 11.137427 5 AAAGGTC 95 1.2985702E-5 11.043888 5 GGACGTG 805 0.0 11.018944 6 GGACTGA 130 3.06718E-8 11.005272 6 GGCGAGG 345 0.0 10.983734 19 GTGTAAC 70 0.0014443923 10.897372 1 GTCTTAC 70 0.0014443923 10.897372 1 CCGTTTC 70 0.0015159037 10.834448 13 GTCCTAC 845 0.0 10.8328905 1 GACGTGG 475 0.0 10.801003 7 >>END_MODULE