FastQCFastQC Report
Thu 26 May 2016
SRR1926494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032773
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19710.1908454229535435No Hit
CTGTAGGACGTGGAATATGGCAAGA14430.13972092608927617No Hit
GTCCTAAAGTGTGTATTTCTCATTT14160.13710660522689883No Hit
GTCCTACAGTGTGCATTTCTCATTT10460.10128072674246906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC1105.9317244E-915.7422911
GTATTAG708.58632E-515.2233151
GTCCTAC11350.015.0221261
TGCCGGT758.693678E-814.71349110
TAGGACC4150.014.4441154
GCATAAG751.544864E-414.2084261
TTGGCGA409.259609E-414.17609317
TCCTACA13200.014.0643442
CCAACGA854.669346E-914.04637319
GTCTTAG955.170172E-614.0214751
GTCCTAA5000.013.8532171
GCCGGTT801.9118306E-713.79470711
TGTAGGA11350.013.7108232
GGACTAT600.0082438313.3204011
GTTGTAA700.00148042213.3204011
GATCTAC600.0082438313.3204011
CCGGTTT751.6687936E-613.27266412
TAAAGCG550.001549839513.240435
CTGTAGG11000.013.19930651
CGTGCAA701.0146139E-513.1370479
Produced by FastQC (version 0.11.5)