##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1032773 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.862240782824493 27.0 10.0 31.0 2.0 31.0 2 16.849460626875413 22.0 2.0 31.0 2.0 31.0 3 20.585328044013544 25.0 15.0 31.0 2.0 31.0 4 22.6258354933756 28.0 7.0 35.0 2.0 37.0 5 23.234980000445404 30.0 7.0 35.0 2.0 37.0 6 24.18489542232417 31.0 10.0 35.0 2.0 37.0 7 23.833223757786076 31.0 10.0 35.0 2.0 37.0 8 24.116532868306976 31.0 16.0 35.0 2.0 37.0 9 26.99133691527567 32.0 17.0 37.0 2.0 39.0 10 27.769590219728826 32.0 25.0 38.0 2.0 39.0 11 24.761616541098576 30.0 10.0 37.0 2.0 39.0 12 25.98498992518201 31.0 17.0 37.0 2.0 39.0 13 22.52614756582521 29.0 2.0 37.0 2.0 39.0 14 25.99344095943639 31.0 17.0 38.0 2.0 40.0 15 27.155787380189064 31.0 19.0 38.0 2.0 40.0 16 27.53699118780216 32.0 23.0 39.0 2.0 40.0 17 26.842994539942467 31.0 17.0 39.0 2.0 40.0 18 26.93622122189484 31.0 18.0 38.0 2.0 40.0 19 27.341276350175693 31.0 23.0 39.0 2.0 40.0 20 27.632770221529803 32.0 25.0 39.0 2.0 40.0 21 25.417448945702493 31.0 10.0 38.0 2.0 40.0 22 26.389675175474185 31.0 17.0 38.0 2.0 40.0 23 26.40025639709791 31.0 11.0 39.0 2.0 40.0 24 25.399737405993378 31.0 2.0 39.0 2.0 40.0 25 25.213275327685754 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.763319732753601 1101 2 10.604277340753658 1101 3 7.097149565624907 1101 4 8.666686672346014 1101 5 8.06810836702071 1101 6 7.920641930455812 1101 7 7.551374456112601 1101 8 5.889878581515131 1101 9 5.691974586630025 1101 10 5.356891277044372 1101 11 8.231405449002704 1101 12 5.907941811047483 1101 13 10.504059300261346 1101 14 7.869784348836085 1101 15 6.893664808835691 1101 16 6.43092255731095 1101 17 2.435671009624887 1101 18 5.042194556565903 1101 19 5.593742663879635 1101 20 3.7398354371613074 1101 21 0.9294453587764373 1101 22 4.865548965171744 1101 23 5.871715587489486 1101 24 7.228100808432551 1101 25 7.8393455473351885 1102 1 6.026635960245709 1102 2 11.139504468312133 1102 3 7.263346055344265 1102 4 8.41239323083148 1102 5 8.251486100761593 1102 6 7.0835381456716 1102 7 7.0511061841764615 1102 8 7.182808790306101 1102 9 6.285269657717414 1102 10 5.404836550815727 1102 11 8.269262327015316 1102 12 5.841353974578077 1102 13 10.340760521350447 1102 14 7.652473175200896 1102 15 5.872408074773883 1102 16 6.170702518238592 1102 17 6.920206581361924 1102 18 7.016715265850731 1102 19 5.837906580836847 1102 20 6.194526768643843 1102 21 5.307990770266951 1102 22 6.095852165428489 1102 23 6.535749699465754 1102 24 8.015152403264025 1102 25 7.687722673491546 1103 1 4.687692561657226 1103 2 8.947295964798997 1103 3 5.92320396498857 1103 4 7.2053497430453035 1103 5 6.600022163231273 1103 6 6.257821043188148 1103 7 6.335266552234302 1103 8 5.884293853605161 1103 9 4.822840663773469 1103 10 4.2011825752590255 1103 11 5.11153420783468 1103 12 3.947697964688089 1103 13 8.23912992497782 1103 14 6.247171389682215 1103 15 5.306146709892733 1103 16 4.861651299985926 1103 17 3.1114839861786017 1103 18 1.7056735307209223 1103 19 3.443019016872718 1103 20 4.012890538732886 1103 21 6.095265233124721 1103 22 1.685563133614643 1103 23 3.8957124555178986 1103 24 5.5600344901770065 1103 25 1.4619852276752283 1104 1 5.577475193576838 1104 2 10.526947949641674 1104 3 7.00046420969262 1104 4 7.499935964460363 1104 5 7.325034260182839 1104 6 7.13373334186031 1104 7 7.012801413449274 1104 8 6.972841653845215 1104 9 5.770785438833016 1104 10 4.695026012427146 1104 11 7.82959228577047 1104 12 5.038374199991079 1104 13 9.360044140309842 1104 14 6.9256375590646755 1104 15 6.146115037874079 1104 16 5.600782581760058 1104 17 6.046898833065754 1104 18 3.6015693649706932 1104 19 4.383681741294733 1104 20 3.782817202927795 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2204 6 4.3622437102110965 2204 7 5.5246781268578715 2204 8 5.3770865904139065 2204 9 3.7665018391328076 2204 10 3.4770247586906464 2204 11 5.107934926199594 2204 12 5.296707105819451 2204 13 3.121968087803058 2204 14 4.369228175309946 2204 15 4.45206302908041 2204 16 4.346384181337644 2204 17 -1.063216643322832 2204 18 2.659547170581476 2204 19 3.644910628284528 2204 20 4.010135770865425 2204 21 5.4427935234038 2204 22 5.055204137585537 2204 23 3.734896900059006 2204 24 5.73644341132465 2204 25 5.718655892162772 2205 1 5.905911785981559 2205 2 10.5051258611262 2205 3 7.126010642137068 2205 4 7.7732415875938905 2205 5 7.976117205244758 2205 6 7.375563415224168 2205 7 7.018950175338524 2205 8 6.983704782889305 2205 9 5.657627768081866 2205 10 5.087567955843721 2205 11 7.297968550931117 2205 12 6.3878426625954745 2205 13 9.717703808191587 2205 14 7.195152522388867 2205 15 6.062869931808475 2205 16 5.560741996196349 2205 17 6.311181087658731 2205 18 6.187780621769907 2205 19 4.884707402417522 2205 20 5.184107576470513 2205 21 -0.916014999705709 2205 22 3.6051931473302297 2205 23 4.874490399883104 2205 24 6.704605277500278 2205 25 7.118572814309985 2206 1 4.479160229292798 2206 2 9.302763838113112 2206 3 6.418195087539711 2206 4 1.8745820478850987 2206 5 6.045154992248886 2206 6 6.708542581663647 2206 7 6.696598078822788 2206 8 6.706665024732736 2206 9 5.294217167419728 2206 10 4.331255802869556 2206 11 3.932536104311829 2206 12 5.093444502180564 2206 13 8.551701118488058 2206 14 0.17598241647184665 2206 15 2.6085546611832164 2206 16 3.7078433734978553 2206 17 4.844945767778235 2206 18 -1.0332481087790342 2206 19 1.2148830996522797 2206 20 3.0876519667469076 2206 21 3.743802344490579 2206 22 -1.6183737956652102 2206 23 -2.4366740507598337 2206 24 1.6741685381424531 2206 25 3.3278589229019744 2207 1 1.4936186116168848 2207 2 -14.271139789390947 2207 3 -6.436677605346169 2207 4 -4.1674860233225 2207 5 1.3854346120462147 2207 6 2.0800852113643593 2207 7 3.2393735347134083 2207 8 3.4534260290272023 2207 9 0.9258805614794667 2207 10 0.6051601680010243 2207 11 -1.0742435767085752 2207 12 -2.2455357131771336 2207 13 0.7253993905220959 2207 14 -5.763822171275571 2207 15 -1.7363540131675634 2207 16 -0.46690882057486505 2207 17 0.8155011569578328 2207 18 0.8141828087261906 2207 19 0.12210963535565966 2207 20 0.03524147414457346 2207 21 -4.100612562074158 2207 22 -4.69116345825968 2207 23 -1.5294637428594022 2207 24 0.41590954535792335 2207 25 0.9472865535394774 2208 1 4.5365963935051745 2208 2 6.399356294159968 2208 3 6.242869352913946 2208 4 7.953487788759087 2208 5 3.363166699894194 2208 6 6.012063498371425 2208 7 6.591484006323597 2208 8 6.673730380874442 2208 9 5.164932645627985 2208 10 4.476869959479455 2208 11 6.666825425361701 2208 12 6.117202172865227 2208 13 8.696954800628738 2208 14 2.928324298940204 2208 15 4.7691162097249915 2208 16 5.091201892923188 2208 17 5.890467897376581 2208 18 0.45594119120781684 2208 19 3.241004634679797 2208 20 4.2549183404161575 2208 21 6.949115314079254 2208 22 5.9121176794669985 2208 23 1.7554189770984792 2208 24 3.0871170638018732 2208 25 4.585466418509132 2209 1 3.2196674774883682 2209 2 4.9761008287105994 2209 3 5.4426984585572065 2209 4 0.9818842060295054 2209 5 5.463820305966852 2209 6 5.75164989368206 2209 7 6.302041127690291 2209 8 4.708721937021739 2209 9 4.4264183026058355 2209 10 3.5339097695071793 2209 11 -0.20513060896702484 2209 12 3.163716561997152 2209 13 2.2933204857335525 2209 14 3.7928445555411088 2209 15 4.180861522378088 2209 16 3.716238348464721 2209 17 1.1052437750473452 2209 18 3.3307718818746217 2209 19 3.7453109995579794 2209 20 4.076151604008455 2209 21 6.181829047299619 2209 22 4.766241910730582 2209 23 0.5294583087372793 2209 24 -2.934168649660453 2209 25 0.3090371595508685 2210 1 -2.0429184802355174 2210 2 -14.271139789390947 2210 3 -8.038577447869969 2210 4 -2.210494341463626 2210 5 -0.4754812329344773 2210 6 -1.194647373140242 2210 7 -1.11576502473876 2210 8 -21.485538548629474 2210 9 -13.362991229371232 2210 10 -7.11623633877176 2210 11 -10.160833596246077 2210 12 -4.717113872992606 2210 13 -5.575007368539321 2210 14 -4.213551651996173 2210 15 -5.9285370514780595 2210 16 -5.332223544272747 2210 17 -4.904880575803098 2210 18 -5.229870994552712 2210 19 -10.607500790744876 2210 20 -7.5238094867216105 2210 21 -5.509981742479152 2210 22 -10.13210563639728 2210 23 -8.074322242980287 2210 24 -11.024374630571089 2210 25 -8.505659427550313 2211 1 0.10107746017510877 2211 2 5.065319765122535 2211 3 0.577120146457851 2211 4 1.4752325752445081 2211 5 1.2275136982015056 2211 6 0.07587759024192309 2211 7 -21.219482051117655 2211 8 -13.408164574420798 2211 9 -9.178847102703045 2211 10 -7.36014484619794 2211 11 -10.002797071187109 2211 12 -4.996876229578614 2211 13 -7.0082919249504645 2211 14 -5.020595348808946 2211 15 -5.61926606264193 2211 16 -12.359786019497724 2211 17 -7.72546021994242 2211 18 -6.070223565953974 2211 19 -8.847433346458804 2211 20 -6.928195473828758 2211 21 -4.660295489137162 2211 22 -6.1230256787924375 2211 23 -7.938966986739793 2211 24 -22.917060481890033 2211 25 -22.726282928749352 2212 1 -0.829113853096441 2212 2 -14.271139789390947 2212 3 -7.960831773211128 2212 4 -20.109175396619502 2212 5 -20.57260353509275 2212 6 -21.52318454340403 2212 7 -10.013161240145681 2212 8 -9.516546300567459 2212 9 -5.029605344161688 2212 10 -5.441628976361152 2212 11 -5.397165818482598 2212 12 -2.782071209449022 2212 13 -6.407691847074561 2212 14 -3.583312015440658 2212 15 -3.650949461442753 2212 16 -4.043396287652282 2212 17 -4.135935088186038 2212 18 -3.313208617835123 2212 19 -3.9306487124790443 2212 20 -4.066067630117448 2212 21 -1.7823967264132925 2212 22 -3.1790344618521225 2212 23 -3.3207798175288765 2212 24 -2.233697333410607 2212 25 -2.6994492972645574 2213 1 -19.31510073443216 2213 2 -14.271139789390947 2213 3 -8.519973653862374 2213 4 -5.378954903753094 2213 5 -20.57260353509275 2213 6 -10.625000367009738 2213 7 -21.219482051117655 2213 8 -10.483593023337646 2213 9 -8.014893831802905 2213 10 -6.743623922867908 2213 11 -6.425679307225931 2213 12 -4.719663584196404 2213 13 -19.842992980050113 2213 14 -12.363205469797963 2213 15 -6.681585974207579 2213 16 -5.799205600650989 2213 17 -4.368111547446016 2213 18 -4.468781033836461 2213 19 -5.368287492123073 2213 20 -5.269505536885372 2213 21 -3.3603325902056405 2213 22 -4.235999952850733 2213 23 -4.090320339444752 2213 24 -3.1369048398666877 2213 25 -4.092690192043776 2214 1 -19.31510073443216 2214 2 -14.271139789390947 2214 3 -12.76284144093084 2214 4 -20.109175396619502 2214 5 -20.57260353509275 2214 6 -21.52318454340403 2214 7 -16.887567157500634 2214 8 -15.417453442246495 2214 9 -14.458636415350108 2214 10 -14.553641308413068 2214 11 -15.113716396388941 2214 12 -12.998135863910509 2214 13 -19.842992980050113 2214 14 -17.36329653693928 2214 15 -24.6491605526771 2214 16 -19.015732906995332 2214 17 -24.402482494268327 2214 18 -18.508566336151773 2214 19 -15.760210112649055 2214 20 -14.385853468557418 2214 21 -11.839240888353944 2214 22 -12.837266613998178 2214 23 -12.75786732181084 2214 24 -11.612805162741097 2214 25 -11.399332574139423 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 166050.0 3 4.0 4 708.0 5 2925.0 6 4255.0 7 4017.0 8 3435.0 9 3140.0 10 2906.0 11 2790.0 12 2752.0 13 3116.0 14 4622.0 15 10567.0 16 15615.0 17 16513.0 18 16688.0 19 19482.0 20 23271.0 21 27580.0 22 27820.0 23 30593.0 24 32319.0 25 33200.0 26 27741.0 27 21368.0 28 22884.0 29 26567.0 30 25917.0 31 30495.0 32 39366.0 33 49911.0 34 61216.0 35 71206.0 36 71059.0 37 74296.0 38 56379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0867655696563 18.822753323959205 12.081946496271113 26.008534610113383 2 13.166785759256982 20.080224409884835 42.67504714397763 24.077942686880547 3 18.97949735517627 24.344491242261338 29.483548508623265 27.19246289393913 4 11.020143854210973 17.437020294240106 38.912687519429525 32.6301483321194 5 11.749872746269457 39.2084371455872 36.383627579678695 12.65806252846465 6 31.375057072646257 38.0307189623938 18.422766972526954 12.171456992432988 7 27.149795694803164 32.06593286916673 22.458089941265595 18.326181494764512 8 23.411547725408443 39.542103585740634 20.498440414426558 16.547908274424366 9 26.373468713132546 15.309039548570283 20.217203535979728 38.100288202317444 10 13.90331606003756 29.86439168877013 35.820484825018205 20.411807426174107 11 33.06544355318983 21.939649330326887 25.8754420335391 19.119465082944178 12 23.766334647845643 24.459381980563847 33.00395175691866 18.770331614671846 13 28.598360957812396 21.969061811883286 25.5132947604087 23.919282469895613 14 19.265405828881427 23.40380928384991 28.100554700386994 29.230230186881673 15 21.430402588761748 33.82407951424807 23.349529719587387 21.3959881774028 16 20.373012657924722 28.30641636849468 30.43898632832378 20.88158464525682 17 20.301644144076764 29.36433143758645 29.919094189999512 20.414930228337276 18 20.18777957729928 27.647632055869735 32.77813669873355 19.386451668097436 19 22.762385871114212 26.87829855821679 27.411473331433236 22.947842239235765 20 22.713623668069005 28.426116918582743 29.231976443807373 19.62828296954088 21 21.133933376372113 26.828633018717817 26.985822539011075 25.051611065898992 22 21.816472765211913 29.882871710073033 28.135888501742162 20.164767022972892 23 20.835575098904275 29.17125925667416 29.128023475284355 20.86514216913721 24 22.334209430983627 27.448150028795187 29.846826621020167 20.370813919201016 25 21.623969241567316 28.25039116955334 29.394234926642376 20.731404662236965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1009.0 1 1009.0 2 2269.0 3 3529.0 4 3529.0 5 3529.0 6 4984.0 7 6439.0 8 6439.0 9 6439.0 10 7443.5 11 8448.0 12 8448.0 13 8448.0 14 10282.0 15 12116.0 16 12116.0 17 12116.0 18 17815.0 19 23514.0 20 23514.0 21 23514.0 22 33425.5 23 43337.0 24 43337.0 25 43337.0 26 57858.5 27 72380.0 28 72380.0 29 72380.0 30 88271.0 31 104162.0 32 104162.0 33 104162.0 34 114043.5 35 123925.0 36 123925.0 37 123925.0 38 126875.5 39 129826.0 40 129826.0 41 129826.0 42 129863.5 43 129901.0 44 129901.0 45 129901.0 46 126369.0 47 122837.0 48 122837.0 49 122837.0 50 110549.0 51 98261.0 52 98261.0 53 98261.0 54 82553.0 55 66845.0 56 66845.0 57 66845.0 58 54481.0 59 42117.0 60 42117.0 61 42117.0 62 33216.5 63 24316.0 64 24316.0 65 24316.0 66 18142.5 67 11969.0 68 11969.0 69 11969.0 70 8471.0 71 4973.0 72 4973.0 73 4973.0 74 3476.5 75 1980.0 76 1980.0 77 1980.0 78 1321.5 79 663.0 80 663.0 81 663.0 82 414.0 83 165.0 84 165.0 85 165.0 86 102.5 87 40.0 88 40.0 89 40.0 90 29.0 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.959769474995959 2 36.17658478678277 3 5.674625498536464 4 13.72344164690595 5 13.257995706704184 6 14.536204955009474 7 9.313179178774037 8 7.338301833994498 9 0.11677299851951978 10 1.4727340858058837 11 5.699703613475565 12 5.764577501541965 13 15.924215679534612 14 1.6198138409892588 15 2.9324933939984876 16 2.9585397759236542 17 2.9049946115942227 18 1.5333475991335948 19 1.4798024348041632 20 0.2967738312291278 21 4.460418698010114 22 1.6260107497000793 23 0.11812857230001172 24 9.041677115881225 25 0.1211301999568153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1032773.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.66188893463769 #Duplication Level Percentage of deduplicated Percentage of total 1 95.96889037352739 86.04751989851631 2 2.9695646152929243 5.325135454412485 3 0.48265545790497155 1.2982740018111676 4 0.1888120515812593 0.6771698079359981 5 0.09645080967166021 0.4323980892218139 6 0.06319447958049688 0.3399681845657245 7 0.037056814872670006 0.23258088135693378 8 0.030430421610297853 0.21827592661253797 9 0.020923582865675965 0.1688443166715234 >10 0.12092196874470078 1.9909921521349694 >50 0.012142536417370222 0.7662462609030396 >100 0.007936483425680218 1.5566756546243334 >500 4.535131133081742E-4 0.245908539388838 >1k 5.668913916352177E-4 0.7000108318443731 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1971 0.1908454229535435 No Hit CTGTAGGACGTGGAATATGGCAAGA 1443 0.13972092608927617 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1416 0.13710660522689883 No Hit GTCCTACAGTGTGCATTTCTCATTT 1046 0.10128072674246906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 9.682669860656698E-5 0.0 0.0 0.0 5 0.0 9.682669860656698E-5 0.0 0.0 0.0 6 0.0 9.682669860656698E-5 0.0 0.0 9.682669860656698E-5 7 0.0 9.682669860656698E-5 0.0 0.0 9.682669860656698E-5 8 0.0 9.682669860656698E-5 0.0 0.0 9.682669860656698E-5 9 0.0 9.682669860656698E-5 0.0 0.0 9.682669860656698E-5 10 9.682669860656698E-5 9.682669860656698E-5 0.0 0.0 1.9365339721313395E-4 11 9.682669860656698E-5 9.682669860656698E-5 0.0 0.0 1.9365339721313395E-4 12 9.682669860656698E-5 9.682669860656698E-5 0.0 0.0 1.9365339721313395E-4 13 9.682669860656698E-5 9.682669860656698E-5 0.0 0.0 1.9365339721313395E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCTAC 110 5.9317244E-9 15.742291 1 GTATTAG 70 8.58632E-5 15.223315 1 GTCCTAC 1135 0.0 15.022126 1 TGCCGGT 75 8.693678E-8 14.713491 10 TAGGACC 415 0.0 14.444115 4 GCATAAG 75 1.544864E-4 14.208426 1 TTGGCGA 40 9.259609E-4 14.176093 17 TCCTACA 1320 0.0 14.064344 2 CCAACGA 85 4.669346E-9 14.046373 19 GTCTTAG 95 5.170172E-6 14.021475 1 GTCCTAA 500 0.0 13.853217 1 GCCGGTT 80 1.9118306E-7 13.794707 11 TGTAGGA 1135 0.0 13.710823 2 GGACTAT 60 0.00824383 13.320401 1 GTTGTAA 70 0.001480422 13.320401 1 GATCTAC 60 0.00824383 13.320401 1 CCGGTTT 75 1.6687936E-6 13.272664 12 TAAAGCG 55 0.0015498395 13.24043 5 CTGTAGG 1100 0.0 13.1993065 1 CGTGCAA 70 1.0146139E-5 13.137047 9 >>END_MODULE