FastQCFastQC Report
Thu 26 May 2016
SRR1926494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032773
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA39570.3831432463861855No Hit
GTCCTACAGTGGACATTTCTAAATT35120.34005536550626325No Hit
GTCCTAAAGTGTGTATTTCTCATTT29570.28631654777961857No Hit
CTTTAGGACGTGAAATATGGCGAGG29370.28438001380748723No Hit
GTCCTACAGTGTGCATTTCTCATTT22490.21776324516616913No Hit
CTGTAGGACCTGGAATATGGCGAGA19080.1847453409413298No Hit
CTGAAGGACCTGGAATATGGCGAGA17470.1691562424656725No Hit
CCCATGTACTCTGCGTTGATACCAC15410.14920994255271972No Hit
GTATCAACGCAGAGTACTTTTTTTT14190.13739708532271855No Hit
GTACATGGGAAGCAGTGGTATCAAC14170.1372034319255054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG402.6813635E-416.6880912
GTTAATC400.005149842714.3026853
TGTCGAG707.44124E-613.53850518
TAGGACC6400.013.4061554
TATGTCG802.052764E-613.03081116
AGGACTT2650.012.950855
CCCTATG603.9612968E-412.7147362
TAGGACT2500.012.2025794
GATATAC4100.012.0862611
GGACTTG2250.011.8636196
TGTAGGA19850.011.6738572
GTATTAA907.200253E-611.647231
ATGTCGA907.657913E-611.58294317
GTAGGAC19300.011.4619093
CCTAGAC1001.8435985E-611.4421473
CTGTAGG19050.011.3554251
ACTGTTC3950.011.3042898
GGACCTG12150.011.22022156
GTCCTAA8250.011.2044431
CCAACGA855.4518237E-511.14935719
Produced by FastQC (version 0.11.5)