FastQCFastQC Report
Thu 26 May 2016
SRR1926493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748202
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA2600.014.7499872
TAGGACC1400.014.5662984
TATAGGA953.868223E-614.4172792
GTAGGAC2450.014.3189863
GTATTAT852.7490634E-514.14244651
GTATTGG955.018337E-614.0597421
TGTTATA600.0068529713.6964152
CTGTAGG2750.013.5996051
CTAATAC652.3938475E-413.4928913
GCTTAGA600.00809173413.3567551
TAAGGTG801.0747266E-513.06183055
GTAGACC952.7459955E-612.6939693
GTCCTAA1502.4710971E-812.4663041
GTCCTAC2400.012.2436911
ATTGTAC902.2019432E-512.1810823
GCACTGT954.7314497E-612.0915596
TCATACA800.003271669411.98436452
ACTATAC650.003661520311.806283
GTATCAA4300.011.8036441
CTAGAAC757.9986994E-411.6938383
Produced by FastQC (version 0.11.5)