##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926493_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 748202 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.23636798618555 27.0 7.0 31.0 2.0 31.0 2 16.321101788019813 16.0 2.0 30.0 2.0 31.0 3 19.949250336139173 23.0 15.0 31.0 2.0 31.0 4 21.97485037463145 28.0 2.0 35.0 2.0 37.0 5 22.841810099411656 30.0 2.0 35.0 2.0 37.0 6 23.22338486130751 30.0 2.0 35.0 2.0 37.0 7 23.198608129890058 30.0 7.0 35.0 2.0 37.0 8 23.325118083084515 30.0 8.0 35.0 2.0 37.0 9 26.159411495825992 31.0 17.0 37.0 2.0 39.0 10 26.9129713633484 32.0 23.0 37.0 2.0 39.0 11 24.00055867265792 30.0 7.0 37.0 2.0 39.0 12 25.160093129930154 31.0 11.0 37.0 2.0 39.0 13 21.794928374957564 27.0 2.0 35.0 2.0 39.0 14 25.17694553075239 31.0 17.0 38.0 2.0 40.0 15 26.26419202300983 31.0 17.0 38.0 2.0 40.0 16 26.64105682689969 31.0 17.0 39.0 2.0 40.0 17 25.948437721364016 31.0 11.0 38.0 2.0 40.0 18 26.026172076524787 31.0 16.0 38.0 2.0 40.0 19 26.430866263388765 31.0 18.0 39.0 2.0 40.0 20 26.670707910430604 31.0 18.0 39.0 2.0 40.0 21 24.518928043496274 30.0 2.0 38.0 2.0 40.0 22 25.457785464353208 31.0 2.0 38.0 2.0 40.0 23 25.44783093335757 31.0 2.0 38.0 2.0 40.0 24 24.50182571016918 31.0 2.0 39.0 2.0 40.0 25 24.305005867399444 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.962349840243988 1101 2 10.441415829302228 1101 3 7.282630698696263 1101 4 8.62738075354638 1101 5 8.393644164532187 1101 6 7.687914808852938 1101 7 7.402194221262167 1101 8 5.8688880145165605 1101 9 5.849624993262523 1101 10 5.5787026631164345 1101 11 8.498137760436535 1101 12 5.927384337137312 1101 13 10.357950390303678 1101 14 7.75309879187844 1101 15 7.040972281183382 1101 16 6.405601365882131 1101 17 2.5921572021988304 1101 18 4.84735685188031 1101 19 5.2890177441537 1101 20 3.5258150984762366 1101 21 0.2778999739344705 1101 22 4.090721748779085 1101 23 5.366681392726559 1101 24 6.7528128577585385 1101 25 7.338781072054623 1102 1 5.78979755789878 1102 2 10.68339191222893 1102 3 6.907630396590953 1102 4 8.243063523634568 1102 5 8.213574293615746 1102 6 6.893104736420167 1102 7 7.042751134277587 1102 8 7.094651313796582 1102 9 6.230466199460274 1102 10 5.663111491358688 1102 11 8.503017365427798 1102 12 5.873920760162086 1102 13 10.364316957638117 1102 14 7.826845719467425 1102 15 5.434294666220225 1102 16 6.056583933197242 1102 17 6.950248670019782 1102 18 7.069744426409688 1102 19 5.96355485839543 1102 20 6.253573501233372 1102 21 5.09374986868098 1102 22 6.176140817182979 1102 23 6.384315279758507 1102 24 8.054275289150421 1102 25 7.745249267615851 1103 1 5.043200422524553 1103 2 9.162691521176443 1103 3 6.477464728765227 1103 4 8.122154026009145 1103 5 7.424655355066587 1103 6 6.789203305816265 1103 7 6.89527789973582 1103 8 6.558108017566543 1103 9 5.592332265052356 1103 10 5.01118970673846 1103 11 5.813317237436998 1103 12 4.523707533713587 1103 13 8.613051850664785 1103 14 6.935493246718114 1103 15 6.078856827207872 1103 16 5.797300327677462 1103 17 3.5677984854188374 1103 18 2.2756332239492387 1103 19 4.367706106046338 1103 20 4.916557914676005 1103 21 7.033841995202053 1103 22 2.7011051268535766 1103 23 4.8369107708446215 1103 24 6.424165041280396 1103 25 2.1644200556839586 1104 1 5.5213404017541485 1104 2 9.892416610353733 1104 3 6.816204150473389 1104 4 7.061647741416554 1104 5 7.437721817989988 1104 6 6.766641036185973 1104 7 6.827568514316454 1104 8 6.973207793940087 1104 9 5.843712998005294 1104 10 4.794296403412105 1104 11 7.803331282414195 1104 12 4.5146103809703 1104 13 8.951848307813346 1104 14 6.463013440828728 1104 15 5.9890723498782705 1104 16 5.473312884980256 1104 17 5.8037374670035184 1104 18 3.540636515369446 1104 19 4.520990040351016 1104 20 3.823673323513173 1104 21 2.692069001007784 1104 22 4.950066606543022 1104 23 5.367431860886946 1104 24 6.581207373459556 1104 25 5.604983185598808 1105 1 3.3963549349479614 1105 2 9.378161623392083 1105 3 6.137138593312262 1105 4 5.343766099746983 1105 5 2.1511230836235526 1105 6 4.351340489737886 1105 7 5.689901797821694 1105 8 2.94789097031493 1105 9 3.605173459413436 1105 10 3.346171600648308 1105 11 6.66851307190711 1105 12 4.108112797632813 1105 13 5.033325544679492 1105 14 3.946283658577496 1105 15 4.196980068249889 1105 16 4.617083542877175 1105 17 4.935416507646632 1105 18 2.2672463780100003 1105 19 2.1961856156163506 1105 20 1.5400684270725584 1105 21 2.4434766446372613 1105 22 3.694485860118185 1105 23 1.1134331564173685 1105 24 4.2954930877452675 1105 25 4.957583381589309 1106 1 6.331582613723764 1106 2 11.041055889759813 1106 3 7.79331983024338 1106 4 8.792410027371595 1106 5 8.503320796639311 1106 6 7.477445083623685 1106 7 7.278769619930873 1106 8 7.047295650469486 1106 9 6.068830886226195 1106 10 5.532058483337657 1106 11 7.688869169848015 1106 12 5.171531930034256 1106 13 4.786928647738851 1106 14 5.844848006094935 1106 15 5.792771389410468 1106 16 6.022295816941909 1106 17 6.325313847227466 1106 18 6.3200807913378405 1106 19 6.190096265913947 1106 20 5.62881109705204 1106 21 5.804301828218492 1106 22 6.283627768364134 1106 23 6.7034972069586125 1106 24 7.371545747956343 1106 25 6.217518112226848 1107 1 4.338134118707803 1107 2 -13.828708009707059 1107 3 -5.520407564924735 1107 4 1.9754693355803177 1107 5 4.873780674237377 1107 6 5.063961786809685 1107 7 4.849986370132264 1107 8 5.402396010689493 1107 9 3.945818847857474 1107 10 3.3734708881141415 1107 11 5.8593395724165696 1107 12 3.0399108127797234 1107 13 4.70875529684848 1107 14 4.269303295499817 1107 15 3.8616978019395773 1107 16 3.610287172587025 1107 17 4.215828130826843 1107 18 -0.6773868544980566 1107 19 2.2571235573937543 1107 20 2.4986553795353075 1107 21 -0.9182478054026575 1107 22 2.311502747906946 1107 23 -0.5918831984378059 1107 24 3.019649720785729 1107 25 3.7891749449856427 1108 1 5.164950067543437 1108 2 10.500405914343576 1108 3 0.3920969368904075 1108 4 5.8602941054294355 1108 5 7.2834529194798705 1108 6 7.3589184534820085 1108 7 7.456128853076251 1108 8 7.326524100263317 1108 9 5.737072577226158 1108 10 4.812811296576481 1108 11 7.087470362231414 1108 12 2.430588083659238 1108 13 3.3947787792208715 1108 14 4.968494410485647 1108 15 4.908423392207403 1108 16 4.763460832120327 1108 17 0.29705981614247534 1108 18 3.8759358384382914 1108 19 4.673016646846023 1108 20 5.10192122380445 1108 21 4.764849850487355 1108 22 2.04113715811404 1108 23 4.837808484469317 1108 24 5.6479024059029115 1108 25 5.894391057554486 1109 1 3.226910143824256 1109 2 -13.828708009707059 1109 3 -5.784340389359484 1109 4 1.694781298981514 1109 5 1.1551351823219136 1109 6 2.0112670997775552 1109 7 3.304078981105718 1109 8 3.520515585195053 1109 9 3.0765216420391788 1109 10 2.2112933829549384 1109 11 0.4549639490872117 1109 12 1.111141142944934 1109 13 2.138478258933393 1109 14 2.9102316664768892 1109 15 2.9734698306073746 1109 16 2.817509224224281 1109 17 3.288113509156524 1109 18 3.2314446067644766 1109 19 1.9516219542918058 1109 20 0.6614047410318236 1109 21 -0.1805882390375686 1109 22 2.1542547514868495 1109 23 2.646207194137702 1109 24 3.911032720526279 1109 25 4.143210548844515 1110 1 2.7981335483405196 1110 2 8.906033774330501 1110 3 6.0838644860090625 1110 4 7.448098835689045 1110 5 8.142728453548017 1110 6 4.11271749493849 1110 7 6.304936266931435 1110 8 7.040380332718861 1110 9 5.395364514670298 1110 10 5.020926871801638 1110 11 2.608502736671653 1110 12 5.292243080239199 1110 13 9.860168203547087 1110 14 7.464905407284672 1110 15 6.662457816341355 1110 16 6.382534170138012 1110 17 6.886296651900132 1110 18 6.803701905345232 1110 19 5.677063597533355 1110 20 5.893194469300422 1110 21 3.632534488971025 1110 22 5.7869733900335625 1110 23 6.423677133943727 1110 24 7.364238895927695 1110 25 7.813855806668013 1111 1 4.837320338237852 1111 2 9.25574639985946 1111 3 7.281086181904417 1111 4 7.948842710998701 1111 5 8.248162252154302 1111 6 7.37768096785371 1111 7 7.321241873365256 1111 8 7.519021988332664 1111 9 6.080196476044922 1111 10 5.348109198447112 1111 11 7.049806764124863 1111 12 6.573694619569853 1111 13 8.255004586067422 1111 14 6.194285674939433 1111 15 5.7614563917499595 1111 16 5.538105866384246 1111 17 6.586932454759143 1111 18 6.626578878193786 1111 19 5.354123465460397 1111 20 4.955160686133059 1111 21 7.720978456212439 1111 22 7.2663975182608205 1111 23 7.159513677870965 1111 24 7.085264601732028 1111 25 7.516621478927782 1112 1 -1.8904658076382077 1112 2 7.545619430723379 1112 3 5.7814343101121075 1112 4 -0.26467477411393503 1112 5 4.081578309022138 1112 6 2.8115515445861057 1112 7 5.7938523408796065 1112 8 5.851291405097275 1112 9 4.443174132648043 1112 10 3.0966853770564136 1112 11 -0.7711835284981525 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1.5708995983697989 2208 19 3.885985543692204 2208 20 4.933842602795529 2208 21 7.247157055832869 2208 22 6.323262535643803 2208 23 2.007925696479994 2208 24 3.52213835259278 2208 25 5.086653893566432 2209 1 2.929899144069882 2209 2 4.975791602395345 2209 3 5.365138198450076 2209 4 1.2148036243728768 2209 5 5.471223083078495 2209 6 5.669334153774361 2209 7 6.223878417377428 2209 8 4.863429826995095 2209 9 4.396248362711564 2209 10 3.4165884891221445 2209 11 -0.22040710718590617 2209 12 3.041252696942955 2209 13 2.0993614008309542 2209 14 3.702842034894484 2209 15 4.1436972918115345 2209 16 3.7039810828009543 2209 17 1.3812922265895402 2209 18 3.375929773025412 2209 19 3.92504610511223 2209 20 4.132682687290373 2209 21 6.226789962333569 2209 22 4.8831094686377625 2209 23 0.6034940754167195 2209 24 -2.950655643275457 2209 25 0.20104620186221211 2210 1 -2.2584865265069 2210 2 -13.828708009707059 2210 3 -7.77644537059917 2210 4 -2.491455950764056 2210 5 -0.8285008752318248 2210 6 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-20.272764504198463 2213 6 -10.692464743530492 2213 7 -20.648115376261053 2213 8 -10.587437464650598 2213 9 -8.428429820466906 2213 10 -7.3760794238177105 2213 11 -6.830697349340603 2213 12 -5.338173004052813 2213 13 -19.201647132916136 2213 14 -12.366564327152483 2213 15 -7.087210678085771 2213 16 -6.18498545140806 2213 17 -4.8486004320454406 2213 18 -4.832782821506122 2213 19 -5.866486080517991 2213 20 -5.739801982693983 2213 21 -3.984053930434296 2213 22 -4.981707396978589 2213 23 -4.641680235036908 2213 24 -3.77817709279514 2213 25 -4.434486665467599 2214 1 -18.761334370282327 2214 2 -13.828708009707059 2214 3 -12.784319765700022 2214 4 -19.435312744905634 2214 5 -20.272764504198463 2214 6 -20.661930392385454 2214 7 -16.807889812351277 2214 8 -15.43346713807079 2214 9 -14.807882746675556 2214 10 -14.790989950771225 2214 11 -15.381119205507739 2214 12 -13.34517205384013 2214 13 -19.201647132916136 2214 14 -17.243473521257336 2214 15 -23.763207285380087 2214 16 -18.72553529931026 2214 17 -23.472858277677332 2214 18 -18.25873558509448 2214 19 -15.833273277518126 2214 20 -14.51349272991828 2214 21 -12.105503387410069 2214 22 -13.046054014157669 2214 23 -13.045802021691841 2214 24 -11.932890370901347 2214 25 -11.706386995172268 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 135223.0 3 1.0 4 562.0 5 2375.0 6 3307.0 7 3196.0 8 2759.0 9 2673.0 10 2450.0 11 2311.0 12 2226.0 13 2380.0 14 3566.0 15 7648.0 16 11102.0 17 11809.0 18 12403.0 19 14638.0 20 17121.0 21 19665.0 22 20537.0 23 22030.0 24 23251.0 25 23782.0 26 19621.0 27 15735.0 28 16997.0 29 19685.0 30 18880.0 31 21719.0 32 27844.0 33 35327.0 34 43022.0 35 49360.0 36 48368.0 37 49962.0 38 34667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.895315630721164 18.165090596992346 11.79443482234098 26.145158949945507 2 14.619585524385325 20.744046425321983 39.80350723126321 24.832860819029484 3 18.704995196653808 23.989384463229605 29.52045885416814 27.78516148594844 4 12.041661119487781 16.489827763965735 38.11453711759297 33.35397399895352 5 12.804666331851466 37.19951885815616 36.64288858714186 13.352926222850517 6 31.928279431926864 37.023065603558074 18.410865191778864 12.637789772736197 7 27.962662158035208 30.823784211232923 22.249270076761814 18.96428355397006 8 25.099946695095948 36.559631964401596 20.83903077778808 17.501390562714377 9 26.17640211611895 15.536803290313431 19.742817156940138 38.54397743662748 10 13.748745626644245 29.518863063111933 35.064820590708145 21.66757071953568 11 33.65398241626184 22.396450374972364 24.49847800332175 19.451089205444045 12 22.857329213771514 24.89900787377012 31.73144956490791 20.512213347550453 13 27.899966310559165 21.26633977796902 25.45644402363689 25.377249887834918 14 20.718490504545734 21.613785892273953 27.96626856145735 29.701455041722962 15 22.796958348770684 30.286769698630817 23.633123201749733 23.283148750848767 16 22.340532506477604 28.436573213786374 26.627390101148407 22.595504178587614 17 21.722805703491158 27.981996221905238 28.248555677330884 22.046642397272723 18 21.565000990558485 27.69900048578074 29.252141913107664 21.48385661055311 19 22.57539983373318 27.341941416034576 27.28213418247851 22.80052456775373 20 22.9653395087787 27.450310313449418 28.008083829526615 21.576266348245266 21 22.343754369284976 26.3538491652918 26.927518805402535 24.374877660020694 22 22.42561623723487 27.499344681324978 27.90937723674185 22.165661844698302 23 22.285432933910617 27.324955981439956 27.872445184182222 22.517165900467212 24 22.143924112419946 27.262689586629108 28.12027687440088 22.473109426550067 25 22.559180702514944 27.095475880819723 27.82349352129863 22.5218498953667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 687.0 1 687.0 2 1494.0 3 2301.0 4 2301.0 5 2301.0 6 3377.0 7 4453.0 8 4453.0 9 4453.0 10 5253.5 11 6054.0 12 6054.0 13 6054.0 14 7264.5 15 8475.0 16 8475.0 17 8475.0 18 11894.0 19 15313.0 20 15313.0 21 15313.0 22 21351.5 23 27390.0 24 27390.0 25 27390.0 26 35754.5 27 44119.0 28 44119.0 29 44119.0 30 53632.0 31 63145.0 32 63145.0 33 63145.0 34 70784.0 35 78423.0 36 78423.0 37 78423.0 38 83839.0 39 89255.0 40 89255.0 41 89255.0 42 91187.0 43 93119.0 44 93119.0 45 93119.0 46 92104.0 47 91089.0 48 91089.0 49 91089.0 50 85380.0 51 79671.0 52 79671.0 53 79671.0 54 69836.5 55 60002.0 56 60002.0 57 60002.0 58 50203.5 59 40405.0 60 40405.0 61 40405.0 62 32099.0 63 23793.0 64 23793.0 65 23793.0 66 17982.0 67 12171.0 68 12171.0 69 12171.0 70 8734.0 71 5297.0 72 5297.0 73 5297.0 74 3684.5 75 2072.0 76 2072.0 77 2072.0 78 1385.5 79 699.0 80 699.0 81 699.0 82 455.0 83 211.0 84 211.0 85 211.0 86 128.0 87 45.0 88 45.0 89 45.0 90 28.0 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.417232244768124 2 36.68728498453626 3 5.672799591554153 4 13.663155137249031 5 13.77569159130823 6 14.896110943301407 7 9.408020828599764 8 7.729730741163482 9 0.10638838174717523 10 1.4410546884397528 11 5.686966888620987 12 5.740294733240488 13 16.29145605063873 14 1.5767132405419926 15 2.9602968182389247 16 2.9723256553711432 17 2.9283535729655896 18 1.502802719051807 19 1.4478710294813433 20 0.2701142204912577 21 4.408167847720268 22 1.5930189975434443 23 0.10558645927169401 24 9.093533564465211 25 0.11093260910823549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 748202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.48130019562998 #Duplication Level Percentage of deduplicated Percentage of total 1 97.39473104013256 92.99375551915537 2 2.06837950162236 3.9498312822578416 3 0.27015930144211336 0.7738548408490834 4 0.10570657515540968 0.4037200494026238 5 0.053978151234197164 0.2576952030998747 6 0.028197731111836506 0.161541361747495 7 0.016050387391079928 0.10727582997207 8 0.011038057609180424 0.0843142473727051 9 0.008479810018982282 0.07286969574219221 >10 0.03887967149776898 0.7006839191614747 >50 0.0024130406418922615 0.14053994878694354 >100 0.001986732142637725 0.3539181024523906 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.336537459135367E-4 0.0 6 0.0 0.0 0.0 1.336537459135367E-4 0.0 7 0.0 0.0 0.0 1.336537459135367E-4 0.0 8 0.0 0.0 0.0 1.336537459135367E-4 0.0 9 0.0 0.0 0.0 2.673074918270734E-4 0.0 10 0.0 0.0 0.0 2.673074918270734E-4 0.0 11 0.0 0.0 0.0 2.673074918270734E-4 0.0 12 0.0 1.336537459135367E-4 0.0 2.673074918270734E-4 0.0 13 0.0 1.336537459135367E-4 0.0 2.673074918270734E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 260 0.0 14.749987 2 TAGGACC 140 0.0 14.566298 4 TATAGGA 95 3.868223E-6 14.417279 2 GTAGGAC 245 0.0 14.318986 3 GTATTAT 85 2.7490634E-5 14.1424465 1 GTATTGG 95 5.018337E-6 14.059742 1 TGTTATA 60 0.00685297 13.696415 2 CTGTAGG 275 0.0 13.599605 1 CTAATAC 65 2.3938475E-4 13.492891 3 GCTTAGA 60 0.008091734 13.356755 1 TAAGGTG 80 1.0747266E-5 13.0618305 5 GTAGACC 95 2.7459955E-6 12.693969 3 GTCCTAA 150 2.4710971E-8 12.466304 1 GTCCTAC 240 0.0 12.243691 1 ATTGTAC 90 2.2019432E-5 12.181082 3 GCACTGT 95 4.7314497E-6 12.091559 6 TCATACA 80 0.0032716694 11.9843645 2 ACTATAC 65 0.0036615203 11.80628 3 GTATCAA 430 0.0 11.803644 1 CTAGAAC 75 7.9986994E-4 11.693838 3 >>END_MODULE