FastQCFastQC Report
Thu 26 May 2016
SRR1926493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748202
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10770.14394508434887904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTG3200.012.8213056
AGGACGT3400.012.3477415
GACGTGA1700.012.2955927
GATATAC701.04933715E-412.2634331
ACGTGGA1800.012.1395318
TAGGACG3550.012.0947794
TTAGGAC2400.011.9275993
TCTGGAC802.7350849E-511.9275993
TGGCGAG3700.011.77512618
GTCCTAC4250.011.67031
TATGGCG3800.011.46602216
CCTAAGG751.9827674E-411.453582
CTAATAC751.9877366E-411.4504953
CTACACT1501.6189006E-1011.4497244
TAGCACA1001.826731E-611.4497234
ATGGCGA3950.011.27039817
TCTAATA600.005669374511.1354252
TTAGTAC600.00568075811.1324253
AAGGCGT600.005683606511.1316766
GACAGTT600.005865566411.0846627
Produced by FastQC (version 0.11.5)