##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 748202 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.30380832983606 31.0 28.0 31.0 22.0 34.0 2 28.773272458507194 31.0 28.0 33.0 23.0 34.0 3 29.139145578333125 31.0 28.0 33.0 25.0 34.0 4 32.924339950975806 35.0 33.0 37.0 27.0 37.0 5 32.564356951732286 35.0 33.0 37.0 25.0 37.0 6 32.678534673791304 35.0 33.0 37.0 26.0 37.0 7 32.487575547779876 35.0 33.0 37.0 25.0 37.0 8 32.45295521797589 35.0 33.0 37.0 25.0 37.0 9 33.68208184420785 37.0 33.0 39.0 23.0 39.0 10 33.578631171795855 37.0 33.0 39.0 22.0 39.0 11 33.82948455096351 37.0 34.0 39.0 25.0 39.0 12 33.6172624505147 37.0 33.0 39.0 23.0 39.0 13 33.772362276497525 37.0 34.0 39.0 25.0 39.0 14 34.4010989010989 38.0 33.0 40.0 19.0 41.0 15 34.526196134199054 38.0 34.0 40.0 23.0 41.0 16 34.496158791342445 38.0 34.0 40.0 23.0 41.0 17 34.41026487499365 38.0 34.0 40.0 22.0 41.0 18 34.37026097230427 38.0 34.0 40.0 21.0 41.0 19 34.41467945822117 39.0 34.0 40.0 19.0 41.0 20 34.385983998973536 39.0 34.0 40.0 19.0 41.0 21 34.31170726621955 39.0 34.0 40.0 18.0 41.0 22 34.242048270386874 39.0 34.0 40.0 18.0 41.0 23 33.8849388266805 39.0 34.0 40.0 15.0 41.0 24 33.96412065190951 39.0 34.0 40.0 16.0 41.0 25 33.75181835921315 39.0 34.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7828550056471784 1101 2 1.0157965545089276 1101 3 1.0396264838276643 1101 4 0.9227816911237525 1101 5 0.9435545751849475 1101 6 0.5918373765850262 1101 7 0.8249144838649016 1101 8 0.9069481590772241 1101 9 1.2161001451159024 1101 10 1.3113228703826039 1101 11 0.8845997016151372 1101 12 1.0242622350481199 1101 13 0.7603500478940006 1101 14 1.227938105302556 1101 15 1.0775635080337693 1101 16 1.032134226051319 1101 17 1.2445653524514881 1101 18 1.291027705594452 1101 19 1.2842329349286175 1101 20 1.2641006050401842 1101 21 1.2688459922740947 1101 22 1.2276743222032138 1101 23 1.632258739414958 1101 24 1.4271664263916506 1101 25 1.4805789907323401 1102 1 0.3844862534871254 1102 2 0.5395420319924114 1102 3 0.4511470886183311 1102 4 0.28082905156922777 1102 5 0.404316533117381 1102 6 -0.17285037944922976 1102 7 0.2555795022449914 1102 8 0.3410414685321541 1102 9 0.5046904733956197 1102 10 0.5992920701418853 1102 11 0.4652751124179417 1102 12 0.39214493601460987 1102 13 0.37067588659876805 1102 14 0.6297369611693213 1102 15 0.47753523097661343 1102 16 0.45564780749770506 1102 17 0.6121157375753441 1102 18 0.4511870238515243 1102 19 0.5405106833014841 1102 20 0.276439794382874 1102 21 0.44614918274787385 1102 22 0.3507042934669542 1102 23 0.5682939818121753 1102 24 0.5848666074131543 1102 25 0.7158543778500857 1103 1 0.40507159319985675 1103 2 0.2920730088789938 1103 3 0.5027761484505184 1103 4 0.42804593829828264 1103 5 0.4738032698646606 1103 6 0.1812648269199144 1103 7 0.5670329394113978 1103 8 0.5860977704464005 1103 9 0.8543043342439205 1103 10 0.9207870173433932 1103 11 0.7071874915148513 1103 12 0.7313736586883905 1103 13 0.5888919621701021 1103 14 0.9361018942533903 1103 15 0.833887987539569 1103 16 0.7736063755876472 1103 17 0.8934257237264589 1103 18 0.7197101369637764 1103 19 0.6121733246971388 1103 20 0.5728279147369548 1103 21 0.6418383891011459 1103 22 0.6039496872934862 1103 23 0.8291309330066028 1103 24 0.5886690999683921 1103 25 0.9275219875104952 1104 1 0.3265444859185678 1104 2 0.5269853525365349 1104 3 0.35837548398763985 1104 4 0.07483971478114881 1104 5 0.27757877837125733 1104 6 -0.049737618474587464 1104 7 0.08946509694327887 1104 8 0.17312909953851374 1104 9 0.3400003210877429 1104 10 0.5207505384263058 1104 11 0.41884363435060123 1104 12 0.448537880356632 1104 13 0.15128279277247003 1104 14 0.6014595183470561 1104 15 0.475965646044628 1104 16 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1.0075632024325643 1107 16 0.7889491328076943 1107 17 0.6589505886224458 1107 18 0.9356675780882284 1107 19 0.976488412830129 1107 20 0.8835413078289136 1107 21 1.1309199430628993 1107 22 0.9207760803504215 1107 23 1.221302113628326 1107 24 1.0385181004985355 1107 25 1.1767414624348476 1108 1 0.9450312019084919 1108 2 0.8909190218836187 1108 3 0.6901339886283857 1108 4 0.5161214254185396 1108 5 -0.5927643494535317 1108 6 0.15379607078888569 1108 7 0.6309947440835799 1108 8 0.7526294695761067 1108 9 1.039433609081101 1108 10 1.00290103395983 1108 11 0.7626809807623687 1108 12 0.762152661038634 1108 13 0.8851589987894855 1108 14 1.2387740186152527 1108 15 1.047877416408575 1108 16 1.0180161870968973 1108 17 0.9504183132227411 1108 18 0.9711625176805185 1108 19 1.069660297079345 1108 20 0.870265122812647 1108 21 0.9399084212430182 1108 22 0.9389942265806894 1108 23 1.0724456736156895 1108 24 0.9603207940681031 1108 25 1.1161454758207157 1109 1 0.5234530432453219 1109 2 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2204 9 0.4297368808120652 2204 10 0.17745684955338703 2204 11 -0.07672708988561538 2204 12 -0.10605400702537082 2204 13 -0.09918873364955516 2204 14 0.33222384022352003 2204 15 -0.005698003168078003 2204 16 0.05815751086660015 2204 17 0.023088769676284926 2204 18 -0.010401354201682977 2204 19 0.031847156192142734 2204 20 -0.0701395328583061 2204 21 0.08906035124351774 2204 22 -0.05306876000143035 2204 23 0.2653965079053435 2204 24 0.2332659309393179 2204 25 0.23466683361279905 2205 1 0.5454856872104195 2205 2 0.6956772024202635 2205 3 0.5209384594188151 2205 4 0.14783441244111373 2205 5 0.22040879513379252 2205 6 0.47344997585814497 2205 7 0.15623992021793498 2205 8 0.34912296950482613 2205 9 0.7919964075110215 2205 10 0.8091467138788104 2205 11 0.43393339390212304 2205 12 0.6727680904795363 2205 13 0.44430257627377756 2205 14 0.7131761364417883 2205 15 0.666225059610916 2205 16 0.5441030868221759 2205 17 0.673189662587447 2205 18 0.704721787346152 2205 19 0.7473392300782606 2205 20 0.6683497188733583 2205 21 0.6633136191417819 2205 22 0.5364305478055797 2205 23 0.9076098409539739 2205 24 0.9006935946960439 2205 25 0.7007185339638227 2206 1 0.28847982551348395 2206 2 0.42661294365348823 2206 3 0.4561350977046992 2206 4 0.3653597180671042 2206 5 0.6417225574086558 2206 6 0.5746793516870596 2206 7 0.6103857136085793 2206 8 0.5059147727497191 2206 9 0.9904402747191909 2206 10 0.9236937551993094 2206 11 0.7202666379012683 2206 12 0.5852566660115883 2206 13 0.413087150321509 2206 14 0.7581610527081679 2206 15 0.366974435989583 2206 16 0.6534257962592989 2206 17 0.7624276419856031 2206 18 0.43936489727529704 2206 19 0.48252178105028776 2206 20 0.5673179090314804 2206 21 0.6895383564649151 2206 22 0.6690437101670241 2206 23 0.8876883175999524 2206 24 0.6118813270508241 2206 25 0.7800936756959445 2207 1 -0.7978461556400838 2207 2 0.0484727344787963 2207 3 0.25083842171837034 2207 4 0.40766063967837596 2207 5 0.3188893794030605 2207 6 0.3712414310077605 2207 7 0.6516045186204877 2207 8 0.6957356280878173 2207 9 0.8634080701007747 2207 10 0.43047639991299036 2207 11 0.3151822431617006 2207 12 0.20786720758764687 2207 13 0.12915839397895468 2207 14 -5.025503646473773 2207 15 -1.880550675328628 2207 16 -0.448588286789267 2207 17 -0.47335986966397314 2207 18 0.10460254871984631 2207 19 0.29079557208269335 2207 20 0.3722521536208987 2207 21 0.5370013366865152 2207 22 0.38916383028714563 2207 23 0.5243663749676699 2207 24 0.24052894018119986 2207 25 0.4318126360355947 2208 1 0.516416188843106 2208 2 1.0135853092775378 2208 3 0.8437266218130794 2208 4 0.8532329845920614 2208 5 0.8660502558936543 2208 6 0.8543702785690002 2208 7 1.0410315279622608 2208 8 1.0852142969930654 2208 9 1.2970384459241018 2208 10 1.32125086286878 2208 11 1.0548180432391874 2208 12 1.0291595576672918 2208 13 0.8743045474939564 2208 14 1.1857667482913925 2208 15 1.1712208172022613 2208 16 1.1941772218421889 2208 17 1.2050162980574086 2208 18 1.2400194844467407 2208 19 1.0103788516039813 2208 20 1.1774353298230622 2208 21 1.1309916074687294 2208 22 1.3249940425218512 2208 23 1.7649363521513592 2208 24 1.540438535945114 2208 25 1.6413093857880554 2209 1 -0.5443846526186427 2209 2 -0.2750029468685291 2209 3 0.2423540163906459 2209 4 0.22096745924505967 2209 5 0.2247244887426234 2209 6 0.3378917494999243 2209 7 0.456203408728058 2209 8 0.6352943462720404 2209 9 0.7214286331319073 2209 10 0.26820739218773326 2209 11 0.31971752400847464 2209 12 0.28004707317592903 2209 13 0.2982217030278207 2209 14 0.49058801030967913 2209 15 0.5247806129966932 2209 16 0.591663296754362 2209 17 0.6732624488638592 2209 18 0.5925646447341606 2209 19 0.6913050406481318 2209 20 0.8960506371047856 2209 21 0.7842826352665995 2209 22 0.8576062059444212 2209 23 0.7362987589179184 2209 24 0.5255543095756323 2209 25 0.7826569449388074 2210 1 -0.31889463601069323 2210 2 -0.4086556039828366 2210 3 0.09058789875636108 2210 4 0.1323753097368865 2210 5 0.7262953319732901 2210 6 0.6747875966285051 2210 7 0.9187793391299479 2210 8 0.8464135302541749 2210 9 1.205308316072177 2210 10 1.133867313623476 2210 11 0.7480694610661729 2210 12 0.6457754842602696 2210 13 0.612789293473611 2210 14 0.9591588364968402 2210 15 0.6542273113222024 2210 16 0.8595487214627795 2210 17 0.9104739796169738 2210 18 1.0813478077691983 2210 19 1.1101359696896935 2210 20 0.9290765350293242 2210 21 0.9957414269072018 2210 22 1.0630088714226673 2210 23 1.2410946124220317 2210 24 1.2589496728579874 2210 25 1.2120258112489068 2211 1 -1.3822578476435439 2211 2 -0.47821123536423826 2211 3 0.2601697775458298 2211 4 0.35095256898254235 2211 5 0.6506128993635087 2211 6 0.4438622442570477 2211 7 0.7230419182002663 2211 8 0.6702783298552077 2211 9 0.8669490415505337 2211 10 0.6661765605853418 2211 11 0.40666351865298367 2211 12 0.5029574312929981 2211 13 0.5231084619557222 2211 14 0.9956525764900164 2211 15 0.5738183767044944 2211 16 0.7558209333318544 2211 17 0.6417989487762696 2211 18 0.6217852208246555 2211 19 0.6187031236832397 2211 20 0.7136561827891086 2211 21 0.9980630101508581 2211 22 1.018016876882129 2211 23 1.302579822007587 2211 24 1.0676887473743761 2211 25 1.1955470322783341 2212 1 1.0724972158254111 2212 2 0.25718579679166353 2212 3 0.6485359308418737 2212 4 0.6043552456612105 2212 5 1.0170337355701875 2212 6 0.9270863905549049 2212 7 0.9959916283118844 2212 8 1.1493099493990542 2212 9 1.2880674743653913 2212 10 0.9719985921027217 2212 11 0.9677718628411753 2212 12 0.9605232627746219 2212 13 0.9865786941218389 2212 14 1.0561556030780181 2212 15 1.110929198352899 2212 16 0.4350785891947524 2212 17 1.0817588496778612 2212 18 1.1217884134280354 2212 19 1.1231778765757383 2212 20 1.1218266933502434 2212 21 1.2359975234704024 2212 22 1.0792139754441834 2212 23 1.4332933050101673 2212 24 1.2806468859589017 2212 25 1.0875929802261268 2213 1 0.43856693552425696 2213 2 0.25951072693295174 2213 3 -0.22000478263365508 2213 4 -0.9347686545803491 2213 5 -1.6817306668079297 2213 6 -0.45055962775039404 2213 7 -0.2450173994281215 2213 8 -0.12479609078557274 2213 9 -0.6293531828605268 2213 10 -0.5221719578419766 2213 11 -0.5010500986826472 2213 12 -1.0603144776511613 2213 13 -0.8735850180300488 2213 14 -7.579865905547798 2213 15 -3.370188299264754 2213 16 -2.3101541959466445 2213 17 -1.585874858988653 2213 18 -5.596739909821874 2213 19 -3.1299876749706215 2213 20 -5.533483273830772 2213 21 -2.416775973131859 2213 22 -1.7943815471513602 2213 23 -4.672474899051814 2213 24 -2.3625186454407476 2213 25 -1.8584742815743027 2214 1 0.0641464696011802 2214 2 -0.23127332607488782 2214 3 -1.765824643991884 2214 4 -2.180159485346831 2214 5 -0.6076395866903326 2214 6 -0.6201263562765433 2214 7 -0.7375025882270911 2214 8 -0.7794184928467054 2214 9 -5.529200606763798 2214 10 -1.7119522291308868 2214 11 -1.1737815456205993 2214 12 -1.4139841161239275 2214 13 -1.4980533134505336 2214 14 -3.008682858872586 2214 15 -2.5303533429171416 2214 16 -1.8309241551959161 2214 17 -2.0685151429045447 2214 18 -1.4187799016589722 2214 19 -4.764252248618483 2214 20 -2.689515051748849 2214 21 -4.266647152352174 2214 22 -6.297093479654372 2214 23 -6.487876156648731 2214 24 -5.786677005718033 2214 25 -3.5205819999797328 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24903.0 3 13.0 4 419.0 5 3054.0 6 3753.0 7 2944.0 8 2276.0 9 1948.0 10 1698.0 11 1605.0 12 1469.0 13 1464.0 14 1342.0 15 1422.0 16 1541.0 17 1565.0 18 1836.0 19 2128.0 20 2728.0 21 3699.0 22 4645.0 23 5688.0 24 6899.0 25 8031.0 26 9185.0 27 10305.0 28 11808.0 29 14356.0 30 17551.0 31 22286.0 32 28399.0 33 36243.0 34 47704.0 35 62493.0 36 83869.0 37 121921.0 38 195012.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.72215994370855 18.790884209230835 8.830587876469156 30.656367970591464 2 14.097641324024481 21.27514499399702 38.02733293937393 26.59988074260457 3 18.175740989072843 24.277193887851244 28.953022449271543 28.59404267380437 4 12.217762637174578 17.04267118745588 36.75730528162235 33.98226089374719 5 13.38027509996404 38.19501911047714 34.92464763595577 13.500058153603051 6 32.368450005769446 37.73228714112056 17.137793067545413 12.761469785564586 7 28.701971053706693 31.65647541038504 21.182364326807804 18.45918920910047 8 25.748655400834558 37.20363560860275 19.45452475201104 17.59318423855165 9 26.17703835781966 15.607706126560844 19.301084703893387 38.91417081172611 10 14.28430119715335 29.95503323049613 33.98038785843755 21.780277713912966 11 35.13857405585732 22.90815726444549 23.178943372824087 18.774325306873102 12 22.74908311324487 25.157180586593675 31.160917635234114 20.932818664927343 13 28.83472645087824 21.858535529175278 25.32658292813973 23.980155091806758 14 21.115044934307235 21.7242896714626 27.28199270511351 29.87867268911665 15 23.179847980400776 30.30381198434094 23.023872182712214 23.492467852546067 16 22.791494639709057 28.50477220267126 26.29262470478637 22.411108452833318 17 21.894505823511643 28.412374661509222 27.234039140627715 22.45908037435142 18 21.985396620880184 28.136565519398328 28.114645748433205 21.763392111288283 19 23.261439926435898 27.3607942356965 27.065147803167903 22.312618034699696 20 23.330784981475443 27.517789456238738 27.452164940737454 21.699260621548362 21 23.01236831764684 26.983889377467584 26.992175909714224 23.011566395171357 22 22.83741556424602 27.778327243177642 26.994047062157012 22.390210130419323 23 22.691228693893752 27.445833405729207 27.040060090804474 22.82287780957256 24 22.671004199423148 27.2847561741678 27.642415510332825 22.401824116076227 25 22.73984832972914 27.373089085567802 27.09949986768279 22.787562717020272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 153.0 2 161.5 3 170.0 4 170.0 5 170.0 6 296.0 7 422.0 8 422.0 9 422.0 10 747.5 11 1073.0 12 1073.0 13 1073.0 14 1828.0 15 2583.0 16 2583.0 17 2583.0 18 4764.5 19 6946.0 20 6946.0 21 6946.0 22 11176.5 23 15407.0 24 15407.0 25 15407.0 26 22236.5 27 29066.0 28 29066.0 29 29066.0 30 38638.0 31 48210.0 32 48210.0 33 48210.0 34 57199.0 35 66188.0 36 66188.0 37 66188.0 38 74465.5 39 82743.0 40 82743.0 41 82743.0 42 88898.5 43 95054.0 44 95054.0 45 95054.0 46 98946.0 47 102838.0 48 102838.0 49 102838.0 50 100175.0 51 97512.0 52 97512.0 53 97512.0 54 87311.0 55 77110.0 56 77110.0 57 77110.0 58 66399.5 59 55689.0 60 55689.0 61 55689.0 62 45296.5 63 34904.0 64 34904.0 65 34904.0 66 26808.0 67 18712.0 68 18712.0 69 18712.0 70 13581.5 71 8451.0 72 8451.0 73 8451.0 74 5832.5 75 3214.0 76 3214.0 77 3214.0 78 2210.5 79 1207.0 80 1207.0 81 1207.0 82 810.5 83 414.0 84 414.0 85 414.0 86 258.0 87 102.0 88 102.0 89 102.0 90 67.5 91 33.0 92 33.0 93 33.0 94 17.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08874608728658838 2 0.03207689901924882 3 0.03327978273247064 4 0.03341343647838418 5 0.024458635502177223 6 0.38706124816560233 7 0.22012771951959498 8 0.22681040681527181 9 0.23135463417633204 10 0.01256345211587245 11 0.0013365374591353672 12 0.002405767426443661 13 0.0 14 0.07417782898201288 15 0.0012028837132218304 16 0.002004806188703051 17 0.003742304885579028 18 0.002539421172357198 19 0.0014701912050489039 20 8.019224754812203E-4 21 0.0 22 0.0 23 1.336537459135367E-4 24 5.346149836541468E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 748202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.4970276553399 #Duplication Level Percentage of deduplicated Percentage of total 1 92.5599558025018 80.98721012628539 2 5.79468387055427 10.140352297516781 3 0.8924101755360873 2.3424971342636334 4 0.2943161579091118 1.0300715603194777 5 0.14465529113623468 0.6328454004519186 6 0.07793825136657752 0.4091619201138159 7 0.05081799032547839 0.3112496173428025 8 0.03356798730024231 0.23496792905147199 9 0.024482857735132636 0.19279595522993875 >10 0.1133777032807611 1.6967901038739286 >50 0.0072038746797107485 0.44912304879800813 >100 0.005517240858073069 0.951404365012348 >500 9.19540128539498E-4 0.47711017637810055 >1k 1.5325668808991631E-4 0.1444203653623424 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1077 0.14394508434887904 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 1.336537459135367E-4 6 0.0 0.0 0.0 0.0 1.336537459135367E-4 7 0.0 0.0 0.0 0.0 1.336537459135367E-4 8 0.0 0.0 0.0 0.0 1.336537459135367E-4 9 0.0 0.0 0.0 0.0 1.336537459135367E-4 10 0.0 0.0 0.0 0.0 1.336537459135367E-4 11 0.0 0.0 0.0 0.0 1.336537459135367E-4 12 0.0 1.336537459135367E-4 0.0 0.0 1.336537459135367E-4 13 0.0 1.336537459135367E-4 0.0 0.0 1.336537459135367E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACGTG 320 0.0 12.821305 6 AGGACGT 340 0.0 12.347741 5 GACGTGA 170 0.0 12.295592 7 GATATAC 70 1.04933715E-4 12.263433 1 ACGTGGA 180 0.0 12.139531 8 TAGGACG 355 0.0 12.094779 4 TTAGGAC 240 0.0 11.927599 3 TCTGGAC 80 2.7350849E-5 11.927599 3 TGGCGAG 370 0.0 11.775126 18 GTCCTAC 425 0.0 11.6703 1 TATGGCG 380 0.0 11.466022 16 CCTAAGG 75 1.9827674E-4 11.45358 2 CTAATAC 75 1.9877366E-4 11.450495 3 CTACACT 150 1.6189006E-10 11.449724 4 TAGCACA 100 1.826731E-6 11.449723 4 ATGGCGA 395 0.0 11.270398 17 TCTAATA 60 0.0056693745 11.135425 2 TTAGTAC 60 0.005680758 11.132425 3 AAGGCGT 60 0.0056836065 11.131676 6 GACAGTT 60 0.0058655664 11.084662 7 >>END_MODULE