FastQCFastQC Report
Thu 26 May 2016
SRR1926492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810509
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT452.6428115E-416.7176444
CTATAAA901.605149E-716.3205171
TGTAGGA3700.014.8309412
CTATAGG1005.305719E-714.6884651
TAGGACC2050.014.6789064
CTGTAGG3850.014.2202371
TATTATA600.006780989513.7186212
GTAGGAC3300.013.3061883
ATTATAC500.00887134213.1731263
GTCCTAC3150.012.7172871
GTTGTAT854.3614977E-412.567671
TCCTACA3700.012.2355262
GTCCTAA2051.07320375E-1011.7247171
TATACAG1351.3600584E-811.6274975
TATAGGA958.8530325E-411.5525232
GACTGTC801.9154615E-411.4954627
GTGTAAG1051.9925312E-411.4455571
GTCCACT1051.9925312E-411.4455571
TAGGGCA852.1047487E-411.3792354
GGACGTG2850.011.377846
Produced by FastQC (version 0.11.5)