##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 810509 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.348231790146684 27.0 7.0 31.0 2.0 31.0 2 16.395856184200298 16.0 2.0 30.0 2.0 31.0 3 20.03991812552359 24.0 15.0 31.0 2.0 31.0 4 22.097478251321082 28.0 2.0 35.0 2.0 37.0 5 22.95386726119019 30.0 2.0 35.0 2.0 37.0 6 23.336898171395998 30.0 2.0 35.0 2.0 37.0 7 23.30771897659372 30.0 7.0 35.0 2.0 37.0 8 23.41915265592362 30.0 10.0 35.0 2.0 37.0 9 26.312488818754634 32.0 17.0 37.0 2.0 39.0 10 27.081294593891 32.0 24.0 37.0 2.0 39.0 11 24.158574426687426 30.0 7.0 37.0 2.0 39.0 12 25.337390454640232 31.0 12.0 37.0 2.0 39.0 13 21.95805351945506 28.0 2.0 37.0 2.0 39.0 14 25.369831797055923 31.0 17.0 38.0 2.0 40.0 15 26.451784002398494 31.0 17.0 38.0 2.0 40.0 16 26.832661944531154 31.0 17.0 39.0 2.0 40.0 17 26.15517039292593 31.0 16.0 38.0 2.0 40.0 18 26.246596891582943 31.0 17.0 38.0 2.0 40.0 19 26.63664808163759 31.0 18.0 39.0 2.0 40.0 20 26.879855744970136 31.0 18.0 39.0 2.0 40.0 21 24.720962999793958 30.0 7.0 38.0 2.0 40.0 22 25.66101425153823 31.0 8.0 38.0 2.0 40.0 23 25.668204794764772 31.0 2.0 38.0 2.0 40.0 24 24.697098983478284 31.0 2.0 39.0 2.0 40.0 25 24.50173779686592 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.9073457315085385 1101 2 10.487597403677727 1101 3 7.100652245715526 1101 4 8.568467126457833 1101 5 8.405924568440504 1101 6 7.979388078222417 1101 7 7.508860127957263 1101 8 5.8900061118040625 1101 9 5.98970331449814 1101 10 5.417293477264462 1101 11 8.233263348424352 1101 12 5.616289694478624 1101 13 10.32756958877265 1101 14 7.867362300588805 1101 15 7.143573567163138 1101 16 6.561840177006086 1101 17 2.7191477619633098 1101 18 5.059997747064305 1101 19 5.779749286613388 1101 20 3.7589750181552724 1101 21 1.0073890890877877 1101 22 4.489741762969203 1101 23 5.822397696953129 1101 24 7.040686287025611 1101 25 7.686147709098041 1102 1 5.758325085247808 1102 2 10.68120348613168 1102 3 6.625666075433031 1102 4 8.100266038432665 1102 5 8.152765493917336 1102 6 6.489582218616992 1102 7 6.621434290636689 1102 8 6.866967244398936 1102 9 6.023681160732654 1102 10 5.450155507807818 1102 11 8.065141174995762 1102 12 5.2993844051032575 1102 13 9.881561068093617 1102 14 7.359104058470432 1102 15 5.204487246130167 1102 16 5.508510164552785 1102 17 6.540849538197172 1102 18 6.556747566819169 1102 19 5.205424058849079 1102 20 5.700832526184374 1102 21 4.620096698709599 1102 22 5.675226196344049 1102 23 5.856075090936219 1102 24 7.393008761955151 1102 25 7.089193655221134 1103 1 4.773720694714481 1103 2 9.07604718050837 1103 3 5.832290638687038 1103 4 7.373748008239506 1103 5 7.052483075827812 1103 6 6.179783022888863 1103 7 6.356232365658116 1103 8 5.9580851822986 1103 9 5.161673118013951 1103 10 4.283711312409309 1103 11 5.221096083031888 1103 12 3.9579649311761678 1103 13 8.260002890230872 1103 14 6.253219161641372 1103 15 5.250296462294713 1103 16 5.004755928940682 1103 17 2.6131965617320105 1103 18 1.0609796190309204 1103 19 3.3578507184838635 1103 20 3.782931543917911 1103 21 6.119051145278259 1103 22 1.8610451692493157 1103 23 3.885393305716363 1103 24 5.344018848315493 1103 25 1.4846433223053168 1104 1 6.062353945276666 1104 2 10.69642426535246 1104 3 7.553567951334909 1104 4 8.128485374652001 1104 5 8.0811233582752 1104 6 7.445328250363023 1104 7 7.660603121805522 1104 8 7.581824387256081 1104 9 6.642584479635971 1104 10 5.561664887317203 1104 11 8.497983887838476 1104 12 5.4745301482489985 1104 13 9.879321529854078 1104 14 7.530971302337672 1104 15 6.758397780040703 1104 16 6.162109010151628 1104 17 6.639412308759944 1104 18 4.339160265231872 1104 19 5.067646281071301 1104 20 4.483545368897218 1104 21 3.433343451956354 1104 22 5.41885678797464 1104 23 5.907613330474458 1104 24 7.13829591924231 1104 25 6.1073350693625486 1105 1 3.767379888600484 1105 2 9.64434726433929 1105 3 6.499200569307952 1105 4 5.797359526504874 1105 5 2.2499054036529884 1105 6 4.850848498436463 1105 7 6.0349146259042605 1105 8 3.134131654456965 1105 9 3.989316234878366 1105 10 3.7249021228948607 1105 11 7.18733330910279 1105 12 4.358141336115505 1105 13 5.0398047818266924 1105 14 4.0946475787799095 1105 15 4.587285440843257 1105 16 4.943138146499916 1105 17 5.530358242259066 1105 18 2.952804304407824 1105 19 2.8296922729045306 1105 20 2.0030504501044284 1105 21 3.0048549192125016 1105 22 4.5561752614633235 1105 23 1.9397707711425376 1105 24 4.779267949576042 1105 25 5.563019573983222 1106 1 6.453103307724142 1106 2 11.208004578819567 1106 3 7.938799421472037 1106 4 9.290931942758945 1106 5 8.78794902736502 1106 6 7.660802852630077 1106 7 7.525705175586822 1106 8 7.488995832163376 1106 9 6.526876637513034 1106 10 5.781831513144201 1106 11 8.185941632399995 1106 12 5.86581042632173 1106 13 4.78945611923395 1106 14 6.14581697378712 1106 15 6.13418167469252 1106 16 6.399160567957903 1106 17 6.573506167382099 1106 18 6.57401101895055 1106 19 6.734948231392124 1106 20 6.2508001267180155 1106 21 6.725487715798732 1106 22 7.024926469516018 1106 23 7.466848993483705 1106 24 8.105238480524491 1106 25 7.15596603308785 1107 1 3.6175653540445616 1107 2 -13.946432877504682 1107 3 -5.800208908718103 1107 4 1.395030080527249 1107 5 4.210806320594145 1107 6 4.428289965542312 1107 7 4.539653638931352 1107 8 4.922484424568836 1107 9 3.15777523959661 1107 10 2.674865029806046 1107 11 5.445658481956585 1107 12 2.6591769751552405 1107 13 4.3790166278755365 1107 14 3.733598289483492 1107 15 3.5278979219257813 1107 16 3.350046363360949 1107 17 3.8663771152226545 1107 18 -1.5133667985922656 1107 19 1.4713908396275777 1107 20 1.7917771185515612 1107 21 -1.6275614729653078 1107 22 1.6233463941378865 1107 23 -1.2111565560778565 1107 24 2.027097262018547 1107 25 2.8381790742651276 1108 1 5.063889625679749 1108 2 10.22552844846217 1108 3 0.3125229312843629 1108 4 5.997816013319657 1108 5 7.031368708382427 1108 6 7.134513911233768 1108 7 7.187545792394602 1108 8 6.813025506578747 1108 9 5.257320035669874 1108 10 4.440066105497426 1108 11 6.9618111900635675 1108 12 2.4417058516125465 1108 13 3.6021416808510267 1108 14 4.838711291016892 1108 15 4.707159270238655 1108 16 4.661060641699947 1108 17 0.24906048746889198 1108 18 3.4743111027307734 1108 19 4.487940382304632 1108 20 4.789689884295878 1108 21 4.555912871487099 1108 22 2.1575878236670007 1108 23 4.8254080894294376 1108 24 5.4602277473896095 1108 25 6.072400084151319 1109 1 3.7061101477689284 1109 2 -13.946432877504682 1109 3 -5.677690463938378 1109 4 2.2820598877060654 1109 5 1.8383944226094613 1109 6 2.8837072608609944 1109 7 3.9135167509273288 1109 8 4.0499506847504065 1109 9 3.6257764631997063 1109 10 2.7616418207513824 1109 11 1.0547592892235436 1109 12 1.4117493932749348 1109 13 2.8744721867147724 1109 14 3.502311898957082 1109 15 3.6053929575191503 1109 16 3.520835950291211 1109 17 4.029511924138369 1109 18 4.150412994328466 1109 19 2.6006116222150837 1109 20 1.2576287411218559 1109 21 0.9387313725859094 1109 22 2.8865029790409054 1109 23 3.413157889178869 1109 24 4.341096629477963 1109 25 4.794815824873041 1110 1 3.016249633322211 1110 2 8.98008009079503 1110 3 6.280233536790121 1110 4 7.478275993895068 1110 5 8.06455171346148 1110 6 4.093370010076253 1110 7 6.5792378012471175 1110 8 7.384343194097653 1110 9 5.725370969482981 1110 10 5.291862083652724 1110 11 3.2837657492687526 1110 12 5.890409817730973 1110 13 10.453846108701423 1110 14 7.770271467430344 1110 15 6.600240455456863 1110 16 6.364453575608589 1110 17 6.753379273591463 1110 18 6.9015544832456825 1110 19 6.05710862788235 1110 20 6.254468889820739 1110 21 4.112307859306927 1110 22 6.095106730795187 1110 23 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12 3.6672547363224233 1112 13 7.76569355576099 1112 14 5.213108286800242 1112 15 3.6575386908095204 1112 16 3.8048822575760397 1112 17 4.776531004018171 1112 18 4.808823676755747 1112 19 4.511386003880794 1112 20 4.176068607932084 1112 21 -1.7240611589366317 1112 22 2.2676772181904177 1112 23 3.75651765612297 1112 24 5.37318635180716 1112 25 5.367259899054819 1113 1 -18.93403855111583 1113 2 -13.946432877504682 1113 3 -7.717667078060359 1113 4 -6.163560775702756 1113 5 -1.6184111988261236 1113 6 -1.0406934760147877 1113 7 -20.806929345726946 1113 8 -9.81211179525577 1113 9 -5.403591566540072 1113 10 -4.746762754938334 1113 11 -5.762134249876102 1113 12 -3.770706234227738 1113 13 -2.5586326195244027 1113 14 -2.85910581474241 1113 15 -3.2164353327746085 1113 16 -3.583611227408369 1113 17 -3.2845764127065245 1113 18 -4.15747201775056 1113 19 -3.655722420784045 1113 20 -4.570456926203001 1113 21 -1.9039335144007303 1113 22 -3.0848995117757276 1113 23 -3.2864703636092365 1113 24 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7 5.362623323825723 2204 8 5.478649784081476 2204 9 3.869135706187201 2204 10 3.3993272249795368 2204 11 5.241851131705719 2204 12 5.103678777397629 2204 13 3.3223229728555204 2204 14 4.536743308109681 2204 15 4.820446842089762 2204 16 4.561098909141531 2204 17 -1.0965184271707962 2204 18 2.868741794813399 2204 19 3.838209051634074 2204 20 4.000139885491734 2204 21 5.624541143154044 2204 22 5.1410790101968615 2204 23 3.916992839853968 2204 24 5.561095342041732 2204 25 5.766786728814207 2205 1 6.220790994338717 2205 2 10.543623940677135 2205 3 7.463177439069398 2205 4 7.926498992978118 2205 5 8.1819643575537 2205 6 7.885883354980628 2205 7 7.8827013360912375 2205 8 7.6562967898534815 2205 9 6.464306160732654 2205 10 5.82207028053509 2205 11 8.089828674995761 2205 12 7.185219632375986 2205 13 10.345117886275435 2205 14 7.927018831197703 2205 15 6.851617927948347 2205 16 6.259691982734605 2205 17 6.961889310924441 2205 18 6.796997566819172 2205 19 5.4739751952127165 2205 20 5.9860029807298325 2205 21 -0.9704714831085823 2205 22 4.05805005998041 2205 23 5.6389557727544 2205 24 7.402883761955152 2205 25 7.6917732006756765 2206 1 4.36079645605777 2206 2 8.808947323356149 2206 3 6.384591861268429 2206 4 1.7361163058123417 2206 5 5.891736147367187 2206 6 6.453167454041537 2206 7 6.653615847960285 2206 8 6.5980255462119 2206 9 5.216207572627791 2206 10 4.3672996319763335 2206 11 4.30620547654787 2206 12 5.212667827563148 2206 13 8.470254429251828 2206 14 0.9560749727785058 2206 15 3.0768414915977544 2206 16 3.8998543669787686 2206 17 4.931194653626935 2206 18 -0.3908162782310427 2206 19 1.7423198459887708 2206 20 3.0503799371665075 2206 21 3.7501371317342524 2206 22 -1.5915164670184154 2206 23 -2.4772881541400835 2206 24 1.4280545425733884 2206 25 3.068959926252827 2207 1 1.4511838305197529 2207 2 -13.946432877504682 2207 3 -6.297617607885083 2207 4 -4.910225157602291 2207 5 1.1607461904087941 2207 6 1.7817183866096862 2207 7 2.982166780528434 2207 8 3.1181116294256768 2207 9 0.7692922211213329 2207 10 0.5035980537840814 2207 11 -1.316463103079105 2207 12 -2.2679634493378558 2207 13 0.3576288912317409 2207 14 -5.300739948311882 2207 15 -1.4540049933376835 2207 16 -0.38995911939139205 2207 17 0.7030741371276505 2207 18 0.7167877389855981 2207 19 0.04648053465448143 2207 20 0.010925130853738807 2207 21 -3.7577538295283013 2207 22 -4.025519336458874 2207 23 -1.0296554425188482 2207 24 0.8003644421429712 2207 25 1.2762769994235583 2208 1 4.888272738129327 2208 2 6.4169605760691315 2208 3 6.079591137678737 2208 4 8.013769651719844 2208 5 3.562365785409412 2208 6 6.42411844258007 2208 7 6.889129438508192 2208 8 6.80809944820048 2208 9 5.37897356372045 2208 10 4.673228563009133 2208 11 6.860989842026793 2208 12 6.2373053378778955 2208 13 8.705591674761497 2208 14 3.3417588225440333 2208 15 4.838229882918895 2208 16 5.117627550840513 2208 17 6.220461853737341 2208 18 0.983766938896661 2208 19 3.3236532444930944 2208 20 4.5518970795334965 2208 21 7.1156040771027484 2208 22 6.153150772769624 2208 23 2.0648990418683866 2208 24 3.7096478583297205 2208 25 4.978582614958789 2209 1 2.634127044887027 2209 2 4.598178114643785 2209 3 4.840569196580265 2209 4 0.3903128880855107 2209 5 4.708881514941279 2209 6 5.2812416826362 2209 7 5.736967870547144 2209 8 4.387478048370127 2209 9 3.9607910173285923 2209 10 3.19289459028138 2209 11 -0.9377840083148605 2209 12 2.8737502476832297 2209 13 2.2096576225028066 2209 14 3.4971488316843704 2209 15 3.8673073097211095 2209 16 3.3558327263592886 2209 17 0.8648913265301807 2209 18 3.052953134270993 2209 19 3.658869000773681 2209 20 3.683626634288977 2209 21 5.902308106145846 2209 22 4.5957032581829935 2209 23 -0.30931119158925213 2209 24 -3.5303467633190024 2209 25 -0.50280271101078 2210 1 -2.299855642570101 2210 2 -13.946432877504682 2210 3 -7.7702009001700745 2210 4 -2.4871397132369566 2210 5 -0.5560299265542454 2210 6 -1.466614046886619 2210 7 -1.2252202002996597 2210 8 -20.97012366469197 2210 9 -13.18919876294187 2210 10 -7.359891650431241 2210 11 -10.0989395119335 2210 12 -4.806028706522749 2210 13 -5.51006602744952 2210 14 -4.037142908954948 2210 15 -5.945064341712275 2210 16 -5.188123882060271 2210 17 -4.749523093668714 2210 18 -4.8127756715616385 2210 19 -10.354180684233922 2210 20 -7.1881847833426775 2210 21 -5.23492306413625 2210 22 -9.789170150936858 2210 23 -7.984371413084499 2210 24 -10.759757218690888 2210 25 -8.338154307161313 2211 1 0.2148192233867512 2211 2 5.024090335465106 2211 3 1.0219750457552657 2211 4 1.8511191234463986 2211 5 1.2318388509214238 2211 6 0.07577153998096264 2211 7 -20.806929345726946 2211 8 -13.350374217381729 2211 9 -9.301528191447957 2211 10 -7.125843420309014 2211 11 -9.959750631797297 2211 12 -4.950668251157211 2211 13 -6.9768919365288795 2211 14 -5.0008305566565205 2211 15 -5.447884965298787 2211 16 -11.513194397649935 2211 17 -7.792569367142146 2211 18 -5.911573730159457 2211 19 -8.959560197866939 2211 20 -6.704816298093778 2211 21 -4.377148561557032 2211 22 -5.851744795817808 2211 23 -7.808946413719774 2211 24 -22.246263965317574 2211 25 -22.07953361750614 2212 1 -1.32357343483676 2212 2 -13.946432877504682 2212 3 -8.036591324144135 2212 4 -19.616824870658245 2212 5 -20.4195981424463 2212 6 -20.813690508655736 2212 7 -10.168847950378108 2212 8 -9.74337947864546 2212 9 -5.530771799098211 2212 10 -5.847905274433195 2212 11 -6.130160093503179 2212 12 -3.3577408591673574 2212 13 -6.441318424506806 2212 14 -4.287155983812866 2212 15 -4.333454085793722 2212 16 -4.499425353417617 2212 17 -4.823473400492048 2212 18 -3.7248047587622217 2212 19 -4.106676892525126 2212 20 -4.4184962264583305 2212 21 -2.2977706374426212 2212 22 -3.7411538247976033 2212 23 -3.7036215263999352 2212 24 -2.6764965234571108 2212 25 -3.046103384948001 2213 1 -18.93403855111583 2213 2 -13.946432877504682 2213 3 -8.438934715080801 2213 4 -5.620777439828206 2213 5 -20.4195981424463 2213 6 -10.52515295924862 2213 7 -20.806929345726946 2213 8 -10.4373173405813 2213 9 -8.142326744405686 2213 10 -6.821401804445145 2213 11 -6.290748153067479 2213 12 -4.7184179664382455 2213 13 -19.4006207500882 2213 14 -12.25676945931613 2213 15 -6.786975204462717 2213 16 -5.96096019117844 2213 17 -4.590438999352237 2213 18 -4.505892749386362 2213 19 -5.3363941229691 2213 20 -5.404622019270171 2213 21 -3.6270179257963306 2213 22 -4.56407815148204 2213 23 -4.369474316178408 2213 24 -3.3126671273729116 2213 25 -4.26767590999626 2214 1 -18.93403855111583 2214 2 -13.946432877504682 2214 3 -12.632737004632968 2214 4 -19.616824870658245 2214 5 -20.4195981424463 2214 6 -20.813690508655736 2214 7 -16.710991575631873 2214 8 -15.237193673335014 2214 9 -14.455017668527972 2214 10 -14.385888807427506 2214 11 -15.073898661572164 2214 12 -12.952515964190372 2214 13 -19.4006207500882 2214 14 -17.190306171748787 2214 15 -24.00278548114256 2214 16 -18.727097912763156 2214 17 -23.767514098166465 2214 18 -18.166944740873134 2214 19 -15.541077080461047 2214 20 -14.182652977076408 2214 21 -11.787138313469233 2214 22 -12.82288773466876 2214 23 -12.856911158769133 2214 24 -11.63260795840314 2214 25 -11.425255311542442 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 144329.0 3 3.0 4 561.0 5 2503.0 6 3521.0 7 3288.0 8 2861.0 9 2758.0 10 2558.0 11 2325.0 12 2313.0 13 2542.0 14 3844.0 15 8240.0 16 12092.0 17 12636.0 18 13488.0 19 15619.0 20 18426.0 21 21358.0 22 21942.0 23 24105.0 24 25123.0 25 25442.0 26 21307.0 27 16855.0 28 17995.0 29 20895.0 30 20291.0 31 23267.0 32 30149.0 33 38038.0 34 46464.0 35 54049.0 36 53287.0 37 56092.0 38 39943.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89051278245282 18.157048903300943 11.709017937680548 26.243420376565695 2 14.55492291110174 20.494165612259245 39.724018620054046 25.22689285658497 3 18.953982129338325 23.610387092887102 29.350112510249044 28.085518267525533 4 12.255972129109152 16.2558231481959 37.9554661670076 33.532738555687345 5 12.868818634081478 37.14178454343291 36.50014901762993 13.489247804855683 6 32.30692985847288 36.6957530236925 18.262928265602426 12.734388852232199 7 28.386348584246146 30.71146094241865 21.902825511641915 18.999364961693292 8 25.2998070946308 36.49287321830458 20.705444218197407 17.50187546886722 9 26.203101550775386 15.394857922788555 19.784830686948414 38.61720983948764 10 13.893517068587535 29.412840588787976 34.92527403695584 21.768368305668652 11 33.609495362364406 22.2037939527328 24.604359901482994 19.5823507834198 12 23.224784008997496 24.594332784098132 31.628213648560255 20.552669558344114 13 28.03705733664058 21.187713935798207 25.401353529414372 25.373875198146838 14 20.509259433506593 21.714033364321477 27.75960726603009 30.01709993614184 15 22.85711377147976 30.41753196514374 23.374384111083966 23.350970152292533 16 22.342681014823263 28.182903363740024 26.985003665092034 22.489411956344682 17 21.745535032819415 28.038594616597972 28.216557268610394 21.99931308197222 18 21.604747155554833 27.50998705161679 29.455754258990225 21.42951153383815 19 22.921435309947718 27.155301478483008 27.1596864378219 22.763576773747374 20 23.167425405777966 27.36311451371608 27.861543620590023 21.60791645991593 21 22.20395565877887 26.33163893724789 26.947101497039654 24.51730390693358 22 22.57027013799914 27.663702222015864 27.71891693824359 22.047110701741396 23 22.14513254223429 27.435211112771757 27.845047684571593 22.57460866042236 24 22.51174328246164 26.999757926545666 27.984779291567957 22.503719499424733 25 22.543795286834115 27.115897238259702 27.800094372018446 22.540213102887733 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 742.0 1 742.0 2 1674.5 3 2607.0 4 2607.0 5 2607.0 6 3664.5 7 4722.0 8 4722.0 9 4722.0 10 5559.0 11 6396.0 12 6396.0 13 6396.0 14 7599.0 15 8802.0 16 8802.0 17 8802.0 18 12258.0 19 15714.0 20 15714.0 21 15714.0 22 22176.0 23 28638.0 24 28638.0 25 28638.0 26 37944.0 27 47250.0 28 47250.0 29 47250.0 30 57370.0 31 67490.0 32 67490.0 33 67490.0 34 76181.5 35 84873.0 36 84873.0 37 84873.0 38 91070.5 39 97268.0 40 97268.0 41 97268.0 42 99498.0 43 101728.0 44 101728.0 45 101728.0 46 101182.5 47 100637.0 48 100637.0 49 100637.0 50 93564.5 51 86492.0 52 86492.0 53 86492.0 54 75696.0 55 64900.0 56 64900.0 57 64900.0 58 54254.0 59 43608.0 60 43608.0 61 43608.0 62 34783.0 63 25958.0 64 25958.0 65 25958.0 66 19678.0 67 13398.0 68 13398.0 69 13398.0 70 9675.5 71 5953.0 72 5953.0 73 5953.0 74 4088.0 75 2223.0 76 2223.0 77 2223.0 78 1512.5 79 802.0 80 802.0 81 802.0 82 508.5 83 215.0 84 215.0 85 215.0 86 147.5 87 80.0 88 80.0 89 80.0 90 44.0 91 8.0 92 8.0 93 8.0 94 6.0 95 4.0 96 4.0 97 4.0 98 2.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.554568795658037 2 36.81303970714699 3 5.801662905655582 4 13.871900250336516 5 13.893121482919993 6 15.106926634991098 7 9.462572284823487 8 7.8998505877171015 9 0.1127686429145142 10 1.5125063386094417 11 5.82214386268382 12 5.876554115993777 13 16.484949581065724 14 1.6569834511399626 15 3.0416688772117273 16 3.0495651498009275 17 3.009096752781277 18 1.5701244526587614 19 1.5207727489762606 20 0.2924088443188169 21 4.538135912124357 22 1.6804255103891503 23 0.11326215995133922 24 9.27750339601411 25 0.11684015846832052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 810509.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.82281142900254 #Duplication Level Percentage of deduplicated Percentage of total 1 96.49583316228421 90.53510358469481 2 2.7389734505974723 5.139563791289021 3 0.401887001407016 1.1311850504633323 4 0.1491590459229962 0.5597808415425282 5 0.0625219691821645 0.29329934623740644 6 0.03530054001783702 0.19871975456612884 7 0.0244987856182089 0.16089814603077413 8 0.017274713891598334 0.1296609779153121 9 0.01302570254698229 0.10998972304162176 >10 0.05428334077971885 0.9030902042903706 >50 0.004828639790995936 0.3101872196018164 >100 0.002413647960820624 0.5285213603268734 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.2337925920625188E-4 0.0 0.0 0.0 6 0.0 1.2337925920625188E-4 0.0 0.0 0.0 7 0.0 1.2337925920625188E-4 0.0 0.0 0.0 8 0.0 1.2337925920625188E-4 0.0 1.2337925920625188E-4 0.0 9 0.0 1.2337925920625188E-4 0.0 1.2337925920625188E-4 0.0 10 0.0 1.2337925920625188E-4 0.0 1.2337925920625188E-4 1.2337925920625188E-4 11 0.0 1.2337925920625188E-4 0.0 1.2337925920625188E-4 1.2337925920625188E-4 12 0.0 1.2337925920625188E-4 0.0 2.4675851841250376E-4 2.4675851841250376E-4 13 0.0 1.2337925920625188E-4 0.0 2.4675851841250376E-4 3.701377776187556E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGAGT 45 2.6428115E-4 16.717644 4 CTATAAA 90 1.605149E-7 16.320517 1 TGTAGGA 370 0.0 14.830941 2 CTATAGG 100 5.305719E-7 14.688465 1 TAGGACC 205 0.0 14.678906 4 CTGTAGG 385 0.0 14.220237 1 TATTATA 60 0.0067809895 13.718621 2 GTAGGAC 330 0.0 13.306188 3 ATTATAC 50 0.008871342 13.173126 3 GTCCTAC 315 0.0 12.717287 1 GTTGTAT 85 4.3614977E-4 12.56767 1 TCCTACA 370 0.0 12.235526 2 GTCCTAA 205 1.07320375E-10 11.724717 1 TATACAG 135 1.3600584E-8 11.627497 5 TATAGGA 95 8.8530325E-4 11.552523 2 GACTGTC 80 1.9154615E-4 11.495462 7 GTGTAAG 105 1.9925312E-4 11.445557 1 GTCCACT 105 1.9925312E-4 11.445557 1 TAGGGCA 85 2.1047487E-4 11.379235 4 GGACGTG 285 0.0 11.37784 6 >>END_MODULE