FastQCFastQC Report
Thu 26 May 2016
SRR1926492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810509
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC10400.12831442957450195No Hit
GTACATGGGAAGCAGTGGTATCAAC10250.12646374068640817No Hit
CTGTAGGACGTGGAATATGGCAAGA9580.11819733031958929No Hit
GTATCAACGCAGAGTACTTTTTTTT9520.11745705476435178No Hit
GTCCTACAGTGGACATTTCTAAATT8970.11067119550800793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTAAG500.001448657113.3597762
GACAGTC604.098257E-412.6629977
GGTTACT550.00297601612.1392151
GTCCTAC5450.012.0755761
ATAGGAC1209.3568815E-911.9268893
GGACGTG4650.011.8990226
TATACTG1458.913048E-1111.8424275
GTTCTAA907.124576E-611.6575011
TAGGACC2300.011.6135874
AGGACGT4950.011.56331355
GCATTAG751.9961322E-411.4455461
TAGGACG4950.011.3705914
GCTACAC1354.3983164E-911.3084573
TCCTACA6150.011.1719382
TTAATGT600.00569186811.1296894
CTACCCT600.00569186811.1296894
GACGTGG2750.011.0513437
GGCGAGG2400.011.05073119
GTCCTAT951.299121E-511.0439481
GTAGGAC5950.010.9045853
Produced by FastQC (version 0.11.5)