FastQCFastQC Report
Thu 26 May 2016
SRR1926491_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926491_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408730
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGC114332.7972010862916843No Hit
GAGTAAGCAGTGGTATCAACGCAGA113222.7700437941917646No Hit
CTGCTTACTCTGCGTTGATACCACT69291.6952511437868518No Hit
GCGTTGATACCACTGCTTACTCTGC40430.9891615491889512No Hit
GNGTAAGCAGTGGTATCAACGCAGA26100.6385633547818853No Hit
GNTTACTCTGCGTTGATACCACTGC25670.6280429623467815No Hit
GTATCAACGCAGAGTAAGCAGTGGT24570.6011303305360507No Hit
GTACATGGGAAGCAGTGGTATCAAC18670.4567807599148582No Hit
GAGTACATGGGAAGCAGTGGTATCA17880.4374525970689698No Hit
TGCTTACTCTGCGTTGATACCACTG17560.42962346781493893No Hit
ACGCAGAGTAAGCAGTGGTATCAAC17140.41934773566902356No Hit
CCACTGCTTACTCTGCGTTGATACC16200.39634966848530817No Hit
ACTCTGCGTTGATACCACTGCTTAC15060.3684583955178235No Hit
CNGCTTACTCTGCGTTGATACCACT14490.3545127590340812No Hit
AAGCAGTGGTATCAACGCAGAGTAA13920.34056712255033883No Hit
GTAAGCAGTGGTATCAACGCAGAGT13520.3307807109828004No Hit
TATCAACGCAGAGTAAGCAGTGGTA13180.32246226115039267No Hit
GTGGTATCAACGCAGAGTAAGCAGT13130.3212389597044504No Hit
GGTATCAACGCAGAGTAAGCAGTGG12870.31487779218555034No Hit
CAGTGGTATCAACGCAGAGTAAGCA10300.2520000978641157No Hit
GCAGTGGTATCAACGCAGAGTAAGC9270.22680008807770413No Hit
GNGTTGATACCACTGCTTACTCTGC9140.22361950431825411No Hit
GATACCACTGCTTACTCTGCGTTGA8820.21579037506422333No Hit
TACCACTGCTTACTCTGCGTTGATA8660.21187581043720793No Hit
GTTGATACCACTGCTTACTCTGCGT8220.20111075771291564No Hit
GAGCAAGCAGTGGTATCAACGCAGA8170.19988745626697332No Hit
ACTGCTTACTCTGCGTTGATACCAC7860.192302987302131No Hit
ATACCACTGCTTACTCTGCGTTGAT7500.18349521689134637No Hit
CCCATGTACTCTGCGTTGATACCAC7080.17321948474543097No Hit
GCTNACTCTGCGTTGATACCACTGC6850.16759229809409634No Hit
GCTTACTGCTTACTCTGCGTTGATA6500.15902918797250018No Hit
GCTTTCTCTGCGTTGATACCACTGC6400.15658258508061557No Hit
GCAGTAAGCAGTGGTATCAACGCAG6250.15291268074278863No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA6010.14704083380226554No Hit
GAGAAAGCAGTGGTATCAACGCAGA5950.1455728720671348No Hit
GAGTAAGCAGTAAGCAGTGGTATCA5940.14532821177794633No Hit
GNATCAACGCAGAGTAAGCAGTGGT5920.1448388911995694No Hit
GCTTACTCTGCGTTTATACCACTGC5860.1433709294644386No Hit
GTATCAACGCAGAGTACATGGGAAG5590.13676510165635014No Hit
GAATAAGCAGTGGTATCAACGCAGA5520.13505247963203093No Hit
GAGTAAGCAGTGGTATCAACCCAGA5180.12673402979962323No Hit
GAGNAAGCAGTGGTATCAACGCAGA5160.1262447092212463No Hit
GCTTCCCATGTACTCTGCGTTGATA5070.12404276661855014No Hit
CACTGCTTACTCTGCGTTGATACCA4840.11841557996721552No Hit
CTCTGCGTTGATACCACTGCTTACT4800.11743693881046167No Hit
AGAGTAAGCAGTGGTATCAACGCAG4740.11596897707533091No Hit
GCTTATTCTGCGTTGATACCACTGC4600.11254373302669245No Hit
GCTTGCTCTGCGTTGATACCACTGC4520.11058645071318474No Hit
GCTTCCTCTGCGTTGATACCACTGC4440.10862916839967705No Hit
NNTNNNNCTGCNNTGATACCACTNC4420.10813984782130012No Hit
GTGGTAAGCAGTGGTATCAACGCAG4420.10813984782130012No Hit
TGATACCACTGCTTACTCTGCGTTG4400.10765052724292319No Hit
GAGTAAGCAGTGCTATCAACGCAGA4360.10667188608616934No Hit
GNACATGGGAAGCAGTGGTATCAAC4230.10349130232671934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4210.10300198174834244No Hit
NNGNNNNCAGTNNTATCAACGCANA4180.10226800088077705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATAA300.002308394522.4166131
CTGCTAC554.0360646E-722.0090391
CTTAGTA453.856345E-521.4746042
GTGTAAG1450.021.334431
TGCTACT557.923829E-620.0801492
AGTACGT350.004850239519.7215752
AGTACGC601.678641E-518.4068032
CTTAATA459.5227495E-418.4068032
AGGGCTA309.6564204E-418.3725439
GGCTATC309.6564204E-418.37254311
GGGCTAT309.6564204E-418.37254310
GTACGTG300.00740865618.3288783
TTAGTAC502.4771805E-517.5957223
GAGTACG1001.4570105E-917.4849571
GAGAAAG2550.017.405841
GAGTAGC857.655035E-817.405841
GCAGTGC2600.017.2801917
GAGAAAA500.002296441716.1399611
GTGCTAT2750.016.0342210
GCTACTC555.6082506E-515.9961123
Produced by FastQC (version 0.11.5)