##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926491_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 408730 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.270298240892522 28.0 12.0 31.0 2.0 33.0 2 16.874976145621805 22.0 2.0 31.0 2.0 31.0 3 20.86000048932058 26.0 15.0 31.0 2.0 33.0 4 22.990218481638244 28.0 10.0 35.0 2.0 37.0 5 23.815898025591466 31.0 10.0 35.0 2.0 37.0 6 24.628955545225455 32.0 10.0 35.0 2.0 37.0 7 24.58208597362562 32.0 11.0 35.0 2.0 37.0 8 24.72009394955105 32.0 17.0 35.0 2.0 37.0 9 27.40274998165048 33.0 18.0 38.0 2.0 39.0 10 28.1605974604262 33.0 27.0 39.0 2.0 39.0 11 24.91665647248795 30.0 10.0 37.0 2.0 39.0 12 26.21972940572016 31.0 17.0 37.0 2.0 39.0 13 22.59985565042938 29.0 2.0 37.0 2.0 39.0 14 26.12302987302131 31.0 17.0 38.0 2.0 40.0 15 27.203601399456854 31.0 20.0 38.0 2.0 40.0 16 27.711494140386073 32.0 24.0 39.0 2.0 40.0 17 27.205852274117387 31.0 19.0 39.0 2.0 40.0 18 27.333371663445305 31.0 22.0 38.0 2.0 40.0 19 27.843221686688032 32.0 25.0 39.0 2.0 40.0 20 28.018555036332053 32.0 27.0 39.0 2.0 40.0 21 25.75129302962836 31.0 10.0 38.0 2.0 40.0 22 26.6607785090402 31.0 18.0 38.0 2.0 40.0 23 26.66829936632985 31.0 17.0 39.0 2.0 40.0 24 25.614613559073227 31.0 2.0 39.0 2.0 40.0 25 25.19073226824554 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.803826610001153 1101 2 11.769098274714363 1101 3 8.267413635660027 1101 4 9.656068348288404 1101 5 9.266986488621676 1101 6 8.900027277742936 1101 7 8.536341637528963 1101 8 7.098014929761899 1101 9 6.670376144287378 1101 10 6.119304539493516 1101 11 8.929168197656857 1101 12 6.529358297494724 1101 13 11.25604105615124 1101 14 8.568220321583276 1101 15 7.809240901975077 1101 16 7.386844472050711 1101 17 3.6151984544186924 1101 18 5.6254221924469725 1101 19 6.697754220210115 1101 20 4.4428595249558995 1101 21 1.3330246045102285 1101 22 5.448558628813021 1101 23 6.532599151893358 1101 24 8.024457071592135 1101 25 8.793629777755509 1102 1 6.3777739558548525 1102 2 11.722545663446034 1102 3 7.819673304642105 1102 4 9.019107538752348 1102 5 8.902562339749366 1102 6 7.799015357667468 1102 7 7.707441886221186 1102 8 7.838067240884445 1102 9 6.686391065820697 1102 10 5.883235591718883 1102 11 8.52379130030672 1102 12 5.495450399372782 1102 13 10.405024848278849 1102 14 7.755515579279873 1102 15 5.44317881467553 1102 16 6.167493644505903 1102 17 7.108170755250526 1102 18 7.102285240213032 1102 19 5.54672086115 1102 20 5.868157526842531 1102 21 5.113635186793051 1102 22 5.890231727175081 1102 23 6.560362637014698 1102 24 7.903909948703873 1102 25 7.71530887903327 1103 1 5.218815168353558 1103 2 10.015462373366791 1103 3 6.807510254017515 1103 4 7.928049024616907 1103 5 7.548095057144433 1103 6 7.312129998312475 1103 7 7.171887022731099 1103 8 6.491671171816311 1103 9 5.392280542965235 1103 10 4.6197749183391785 1103 11 5.6770578493222565 1103 12 4.374018861949342 1103 13 8.670648226250403 1103 14 6.656740535160701 1103 15 5.751727553386214 1103 16 5.3125246144356595 1103 17 3.3486462042280003 1103 18 2.1116540765049407 1103 19 4.062145270299101 1103 20 4.493507884986407 1103 21 6.800250640614983 1103 22 2.446410141653093 1103 23 4.671551605491118 1103 24 6.047124246776473 1103 25 2.0706066402014756 1104 1 6.332494637296183 1104 2 11.420196054416262 1104 3 7.959319958458799 1104 4 8.27023331416962 1104 5 8.336541398222355 1104 6 8.076789394550815 1104 7 8.308681712157366 1104 8 7.957334203268818 1104 9 6.711349612034301 1104 10 5.569474618038878 1104 11 8.64248380159031 1104 12 5.501211844405482 1104 13 10.27247373597065 1104 14 7.594744328427652 1104 15 6.792268093926754 1104 16 6.625785243485758 1104 17 7.340941130733455 1104 18 4.65989969054003 1104 19 5.8233670183629584 1104 20 5.207590388542599 1104 21 4.020181097387546 1104 22 6.214112054091846 1104 23 6.643655288121117 1104 24 7.64570967430927 1104 25 6.773032750522322 1105 1 4.227231479401446 1105 2 10.48847485953717 1105 3 7.161311423608158 1105 4 6.3413570695039 1105 5 2.831562735348953 1105 6 5.536248854010278 1105 7 6.957330360806015 1105 8 3.7496528376927145 1105 9 4.579059427112536 1105 10 4.119972484326222 1105 11 7.218586219797995 1105 12 4.563800749099649 1105 13 5.847153679071646 1105 14 4.1324399187548195 1105 15 4.65997790900499 1105 16 4.9529545180234535 1105 17 5.517328043962472 1105 18 3.031165693384981 1105 19 2.8347468192164413 1105 20 1.9401650684856975 1105 21 3.2918738427645025 1105 22 4.50287450074903 1105 23 1.9722470377654986 1105 24 5.174870413996324 1105 25 5.635052665173816 1106 1 6.43707852337031 1106 2 11.335744586389985 1106 3 7.663159442531871 1106 4 8.997649523992688 1106 5 8.564710800622983 1106 6 7.827620503601917 1106 7 7.711040383661516 1106 8 7.378506706345519 1106 9 6.274491923476436 1106 10 5.5399322874173045 1106 11 7.892126184955345 1106 12 5.5287842867472925 1106 13 4.797003440127792 1106 14 5.793395374506254 1106 15 5.895725187921993 1106 16 5.873821348780879 1106 17 6.296630631588535 1106 18 6.20107355216037 1106 19 5.914665146769302 1106 20 5.574377193088125 1106 21 6.211110421152469 1106 22 6.140115378528055 1106 23 6.763648655231577 1106 24 7.471766141776147 1106 25 6.28287420048796 1107 1 3.6053237952928043 1107 2 -14.483075638329115 1107 3 -6.342168350206544 1107 4 1.6048167013845855 1107 5 4.434108299617009 1107 6 4.648967270556444 1107 7 4.476729707956558 1107 8 4.980156503603077 1107 9 2.969693427794386 1107 10 2.491301185798463 1107 11 5.016855345818318 1107 12 2.3675305336906796 1107 13 3.778702452725682 1107 14 3.2014338800932762 1107 15 3.1550188394526195 1107 16 2.879969397191445 1107 17 3.6619345904828933 1107 18 -1.38214757638762 1107 19 1.377560184996316 1107 20 1.746081467703533 1107 21 -1.3839115555089343 1107 22 1.7485825837776865 1107 23 -2.0572918530174107 1107 24 1.7544486873545004 1107 25 2.4193693872895814 1108 1 5.1903302331895596 1108 2 10.684043926888275 1108 3 -0.2705392628906971 1108 4 5.713224145389855 1108 5 7.315718372679651 1108 6 7.2953171328153985 1108 7 7.0914141012970795 1108 8 6.852996813819871 1108 9 5.348365274722159 1108 10 4.4035436699283 1108 11 6.880798632439468 1108 12 0.9989235148860303 1108 13 2.8332149691947173 1108 14 4.436608002742698 1108 15 4.685509017917106 1108 16 4.592098095239113 1108 17 0.12670207760709928 1108 18 3.5867446562150853 1108 19 4.4728447999202565 1108 20 4.967406626405172 1108 21 5.014908662481243 1108 22 1.3687579041753395 1108 23 4.655334659139733 1108 24 5.341667216519671 1108 25 5.65984354587145 1109 1 3.929809151871428 1109 2 -14.483075638329115 1109 3 -6.0379908666997455 1109 4 2.693979219941916 1109 5 1.7883330199669558 1109 6 2.990922320135283 1109 7 4.427587513150229 1109 8 4.61169324910367 1109 9 3.5024773847334387 1109 10 2.7554027727976127 1109 11 1.0131676964665317 1109 12 1.4498695116282931 1109 13 3.0268467196019344 1109 14 3.7338804310098297 1109 15 3.5333899225656467 1109 16 3.3208836484279374 1109 17 4.167380720634288 1109 18 4.290568581788325 1109 19 2.8435337799979408 1109 20 1.8946909579425757 1109 21 1.087988477040824 1109 22 3.5076072025088827 1109 23 3.8865439374256603 1109 24 5.095058082325963 1109 25 5.477633453151238 1110 1 3.631017870731938 1110 2 9.59900370503614 1110 3 6.347789931780092 1110 4 7.803714019296951 1110 5 8.210349759106819 1110 6 3.906637242254522 1110 7 6.501632384768204 1110 8 7.288918793835332 1110 9 5.624072688628065 1110 10 4.901294666181197 1110 11 2.8013028553622057 1110 12 5.6894903381775315 1110 13 10.100262789333897 1110 14 7.300673383697912 1110 15 6.647796384835562 1110 16 6.138606005784524 1110 17 6.681817538857324 1110 18 7.024216776003346 1110 19 5.677922344082511 1110 20 5.892384693971941 1110 21 4.301130633575042 1110 22 6.038219407178968 1110 23 6.645507813841455 1110 24 7.613677714348729 1110 25 8.018541056726146 1111 1 4.796950753655025 1111 2 9.450346465532402 1111 3 7.460491464255714 1111 4 7.398384873115209 1111 5 7.9296642709023075 1111 6 7.069451620165598 1111 7 7.362241404023877 1111 8 7.249831830898547 1111 9 5.512644424261904 1111 10 4.768965860630555 1111 11 6.894559270429394 1111 12 6.208759675367709 1111 13 8.037428192143832 1111 14 5.928092183255636 1111 15 5.223064017048696 1111 16 5.239911879758303 1111 17 6.405838953077485 1111 18 6.062941249163618 1111 19 5.206886005848588 1111 20 4.645866144149629 1111 21 7.296287114400421 1111 22 6.664191890743975 1111 23 6.465654594877591 1111 24 6.7724008759882075 1111 25 7.171662931617988 1112 1 -2.6304994869228757 1112 2 7.6434718169666205 1112 3 5.5806265615217185 1112 4 -1.3554600690710004 1112 5 3.26393842530808 1112 6 2.7676348769694563 1112 7 5.522728017928859 1112 8 5.68543992427977 1112 9 4.335677262641543 1112 10 3.203462185567318 1112 11 -1.109023298067079 1112 12 3.8694157103288873 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4.8252878930493175 2204 7 6.091477666005954 2204 8 5.946349534389409 2204 9 4.256315630684529 2204 10 4.061295386175438 2204 11 5.606882410725763 2204 12 5.7906855286160095 2204 13 3.1764961794667776 2204 14 4.914050476681165 2204 15 5.010499483210776 2204 16 4.844688357125712 2204 17 -0.926207596941591 2204 18 3.262238871826579 2204 19 3.8019113521704497 2204 20 4.125086832354832 2204 21 5.939139529503873 2204 22 5.098456925278825 2204 23 3.7139492663098324 2204 24 5.3073581649051285 2204 25 5.727039388739488 2205 1 6.344381894156829 2205 2 11.084340092007963 2205 3 7.642092026801535 2205 4 8.358604580172464 2205 5 8.632126530960061 2205 6 7.671721627197421 2205 7 7.969467349953646 2205 8 7.757720189491582 2205 9 6.480952597633742 2205 10 5.5329098155071925 2205 11 7.705328432146359 2205 12 7.143952826073132 2205 13 10.475374226644647 2205 14 7.87485604866356 2205 15 6.444882491292777 2205 16 6.000225843162902 2205 17 7.151967710240221 2205 18 6.736185911710937 2205 19 5.115507843398518 2205 20 5.374938990840931 2205 21 -1.3254064327801132 2205 22 3.912587288640413 2205 23 5.4841542732091035 2205 24 7.446485242899737 2205 25 7.883997795993576 2206 1 3.8204035988893565 2206 2 8.448645556549973 2206 3 6.083075292740446 2206 4 1.7396500993758792 2206 5 5.670823048293478 2206 6 5.775224671933465 2206 7 6.09957787147458 2206 8 6.064019836270237 2206 9 4.529272798378539 2206 10 3.679005201253677 2206 11 3.091985935302972 2206 12 4.519704020792396 2206 13 8.238334333410194 2206 14 0.17938159727544445 2206 15 2.0582709388769658 2206 16 3.3697397242823435 2206 17 4.359433307120366 2206 18 -1.2760889296591174 2206 19 1.264334301435504 2206 20 2.7139915265226833 2206 21 3.724306275196934 2206 22 -1.822872298681979 2206 23 -2.6721341855630953 2206 24 1.685538977296467 2206 25 3.408879123210802 2207 1 1.3877863250588796 2207 2 -14.483075638329115 2207 3 -6.374222326020151 2207 4 -4.375152481974428 2207 5 1.469197247648946 2207 6 2.18444142660071 2207 7 3.327105577592828 2207 8 3.6039716971409206 2207 9 0.9212972938820734 2207 10 0.7817830460029747 2207 11 -1.1310995498253327 2207 12 -3.175787858240966 2207 13 0.44280106597683755 2207 14 -5.775908884999872 2207 15 -1.8814125001374258 2207 16 -0.2395618163305251 2207 17 0.8778611908470886 2207 18 1.299507183849574 2207 19 0.41706151571490224 2207 20 0.5390029786527784 2207 21 -4.163794358889426 2207 22 -4.590412140039842 2207 23 -1.0170201062741704 2207 24 0.5929414734589997 2207 25 0.9612053120222726 2208 1 5.282721537537384 2208 2 6.783228709496971 2208 3 6.772938997978866 2208 4 8.574637188868117 2208 5 3.944794459636171 2208 6 6.4678714806414845 2208 7 7.322392362166642 2208 8 7.2959315964285665 2208 9 5.603800057330854 2208 10 4.88044584384134 2208 11 6.874005154178597 2208 12 6.827727862712116 2208 13 9.247617143107764 2208 14 3.6064449592644365 2208 15 5.224911191830149 2208 16 5.689924182195632 2208 17 6.716375990650576 2208 18 1.4698968301281319 2208 19 4.28942088687678 2208 20 5.122297930753 2208 21 7.864093445089942 2208 22 6.632344860697078 2208 23 2.625443354791905 2208 24 4.5169389556501045 2208 25 5.801147893697536 2209 1 2.404007256221952 2209 2 3.770465438158137 2209 3 4.419250889837471 2209 4 -1.1580384793176215 2209 5 4.067635172778182 2209 6 4.246096169642595 2209 7 5.142713522408052 2209 8 4.092308151423023 2209 9 3.405380455163101 2209 10 2.5581270545815826 2209 11 -1.4091000619810252 2209 12 2.4264392187904527 2209 13 1.230200435385136 2209 14 2.728096332586272 2209 15 3.2897475325125 2209 16 2.722386615992903 2209 17 0.44785847001995904 2209 18 2.416422726174442 2209 19 2.92676846662798 2209 20 2.929097567407762 2209 21 5.222230992817494 2209 22 3.3535428222687003 2209 23 -1.4184621446538372 2209 24 -4.689440370621604 2209 25 -1.3355569036913053 2210 1 -2.600813651470485 2210 2 -14.483075638329115 2210 3 -8.3871814703478 2210 4 -2.5416179717563203 2210 5 -1.076126228017035 2210 6 -1.8719677336534453 2210 7 -1.710095381350321 2210 8 -22.278796664441 2210 9 -14.465993356422208 2210 10 -8.534714296785673 2210 11 -10.894544871624523 2210 12 -5.831678988016819 2210 13 -6.0892950295151245 2210 14 -5.203103808636481 2210 15 -7.008619159092312 2210 16 -6.522491957657287 2210 17 -6.185777763703701 2210 18 -6.289186675226016 2210 19 -11.580708773812164 2210 20 -8.754891302894272 2210 21 -6.807598110838327 2210 22 -10.707107306765183 2210 23 -8.80649687872635 2210 24 -11.617958960747778 2210 25 -9.216450641975282 2211 1 -0.3113518675235092 2211 2 4.836227699842148 2211 3 0.47022594503509296 2211 4 1.2252591520157168 2211 5 0.8236575725282016 2211 6 -0.02307909359969429 2211 7 -22.082498942181182 2211 8 -13.824515096951885 2211 9 -8.95570450189171 2211 10 -6.920046757281899 2211 11 -9.483856349576055 2211 12 -4.425226166441664 2211 13 -7.147057322090697 2211 14 -4.4705924926192 2211 15 -4.816869192469721 2211 16 -11.760188615179262 2211 17 -7.569930171399022 2211 18 -5.584161114593268 2211 19 -8.88394788954778 2211 20 -6.268160436761356 2211 21 -3.9779133227525527 2211 22 -5.830461585317938 2211 23 -8.293466770152879 2211 24 -23.226267566089025 2211 25 -22.781732541085074 2212 1 -0.9383454370225444 2212 2 -14.483075638329115 2212 3 -8.229924176120779 2212 4 -20.516395419827536 2212 5 -21.279390322972525 2212 6 -22.0796828671846 2212 7 -10.676204235457291 2212 8 -9.273074203782917 2212 9 -5.283377665503007 2212 10 -5.548767862073941 2212 11 -5.8617256258176695 2212 12 -2.9150218729350783 2212 13 -6.635785777213194 2212 14 -3.9501226528522544 2212 15 -3.9697166149746046 2212 16 -3.7594328184885732 2212 17 -4.113009544586095 2212 18 -3.176257225354856 2212 19 -3.7011945886472617 2212 20 -4.046361100782736 2212 21 -1.5423314942644168 2212 22 -2.9545825044829925 2212 23 -3.079082131928253 2212 24 -2.2620329452020442 2212 25 -2.615781182000667 2213 1 -19.838202375506093 2213 2 -14.483075638329115 2213 3 -9.126418943318622 2213 4 -5.749833905631952 2213 5 -21.279390322972525 2213 6 -11.499241226806053 2213 7 -22.082498942181182 2213 8 -11.452298241728066 2213 9 -8.190342549993243 2213 10 -7.442730846598927 2213 11 -6.359694784101471 2213 12 -5.2998009399212656 2213 13 -20.009176335153107 2213 14 -12.967353216568783 2213 15 -6.966519167516992 2213 16 -6.6606707293391345 2213 17 -5.212881827207056 2213 18 -5.257168215818929 2213 19 -6.184571020954795 2213 20 -6.2511376404992305 2213 21 -4.6121315240500955 2213 22 -5.427964939056039 2213 23 -5.074542072447155 2213 24 -4.114280184385553 2213 25 -4.8889880615898065 2214 1 -19.838202375506093 2214 2 -14.483075638329115 2214 3 -12.555042610265453 2214 4 -20.516395419827536 2214 5 -21.279390322972525 2214 6 -22.0796828671846 2214 7 -17.271080257060074 2214 8 -15.450076941257608 2214 9 -13.608847565656959 2214 10 -13.691527414771565 2214 11 -15.10245509104481 2214 12 -12.15640971815143 2214 13 -20.009176335153107 2214 14 -16.938885641006365 2214 15 -24.800904025561156 2214 16 -18.530541817503476 2214 17 -24.83117835717551 2214 18 -18.248495865824932 2214 19 -15.01122226040771 2214 20 -13.583071972962962 2214 21 -10.949774653300311 2214 22 -11.893019025266513 2214 23 -11.859883672438423 2214 24 -10.717617047057884 2214 25 -10.224639115479537 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 62510.0 3 1.0 4 274.0 5 1126.0 6 1875.0 7 1855.0 8 1456.0 9 1306.0 10 1129.0 11 994.0 12 1023.0 13 1068.0 14 1696.0 15 4100.0 16 6210.0 17 6550.0 18 7022.0 19 7848.0 20 9350.0 21 10597.0 22 11065.0 23 12034.0 24 12636.0 25 13101.0 26 10777.0 27 8013.0 28 8453.0 29 10148.0 30 9679.0 31 11515.0 32 15534.0 33 19771.0 34 24466.0 35 28658.0 36 29002.0 37 31102.0 38 24786.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.02029862233075 12.985572529235506 8.428993019695945 16.5651358287378 2 8.831769535827648 30.287614568539347 27.494897286054652 33.385718609578355 3 41.011028934734654 22.26962501621902 28.659919553652525 8.059426495393799 4 10.549934656457388 14.113921718152463 48.90531848244229 26.430825142947857 5 8.337593002782985 48.209802919293466 31.75924348270574 11.693360595217811 6 20.92688750485451 36.71590696604001 16.24354530155488 26.113660227550596 7 22.033105365492215 36.94539318368042 24.77466879761161 16.246832653215755 8 20.64088755418548 16.90215574644644 19.193995956980576 43.2629607423875 9 15.930315432148657 30.573332647558026 31.393568897150846 22.102783023142468 10 36.26011744167592 22.39500674496112 17.962178622440884 23.382697190922073 11 21.07696738687639 21.140538198273468 28.129175888758862 29.653318526091276 12 45.92976834857629 26.37419896721209 18.906966133681742 8.789066550529874 13 30.6302909124924 18.063350638417287 23.212782376876667 28.09357607221365 14 23.29876476986575 21.190877077963037 44.352311276007136 11.15804687616408 15 33.3939372573115 26.785255619293203 24.02282468006668 15.797982443328614 16 8.742175135832586 35.016582159813716 37.58757295646301 18.65366974789069 17 26.529548744414377 24.00327270438668 29.638114418780287 19.829064132418655 18 15.569108918752605 42.036048196597655 23.51643607201699 18.87840681263275 19 16.558869919747636 39.667880206731674 22.062455980239466 21.71079389328122 20 18.460625004600313 27.205144498884888 12.664476508963862 41.66975398755094 21 23.84406373768076 30.102698719719996 24.021112850900085 22.03212469169916 22 19.631050019622357 17.824054525312842 16.111693434210462 46.43320202085434 23 14.450507465026059 39.33760139503899 30.702123907676633 15.509767232258318 24 43.807826687083484 22.62304009316971 14.734617292470938 18.834515927275863 25 15.711766434799648 32.137503673949254 22.10835700989517 30.042372881355934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1644.0 1 1644.0 2 2489.5 3 3335.0 4 3335.0 5 3335.0 6 3804.5 7 4274.0 8 4274.0 9 4274.0 10 3922.5 11 3571.0 12 3571.0 13 3571.0 14 3039.5 15 2508.0 16 2508.0 17 2508.0 18 2582.0 19 2656.0 20 2656.0 21 2656.0 22 3907.5 23 5159.0 24 5159.0 25 5159.0 26 7434.0 27 9709.0 28 9709.0 29 9709.0 30 13237.0 31 16765.0 32 16765.0 33 16765.0 34 21023.5 35 25282.0 36 25282.0 37 25282.0 38 32033.0 39 38784.0 40 38784.0 41 38784.0 42 57984.0 43 77184.0 44 77184.0 45 77184.0 46 101176.5 47 125169.0 48 125169.0 49 125169.0 50 98440.5 51 71712.0 52 71712.0 53 71712.0 54 44228.0 55 16744.0 56 16744.0 57 16744.0 58 9848.5 59 2953.0 60 2953.0 61 2953.0 62 1838.5 63 724.0 64 724.0 65 724.0 66 513.0 67 302.0 68 302.0 69 302.0 70 225.5 71 149.0 72 149.0 73 149.0 74 109.5 75 70.0 76 70.0 77 70.0 78 45.0 79 20.0 80 20.0 81 20.0 82 13.0 83 6.0 84 6.0 85 6.0 86 6.5 87 7.0 88 7.0 89 7.0 90 4.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.531426614146259 2 36.94957551439826 3 5.720157561226237 4 13.695838328480905 5 13.845325765175055 6 14.952413573752843 7 9.527316321287891 8 7.77628263156607 9 0.10593790521860397 10 1.3373131407041323 11 5.7086585276343795 12 5.621804124972476 13 16.302693709783963 14 1.4806840701685708 15 2.8419739192131726 16 2.915861326548088 17 2.8148166271132533 18 1.397988892422871 19 1.346120911114917 20 0.2811146722775426 21 4.374036650111321 22 1.4992782521468941 23 0.10422528319428473 24 9.246690969588727 25 0.11009713013480783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 408730.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.05603890583424 #Duplication Level Percentage of deduplicated Percentage of total 1 86.45803622923421 34.63166462920233 2 6.250208226189711 5.007171677556407 3 2.194147179813137 2.6366653439916443 4 1.1573149742713913 1.8542981454287764 5 0.7183663906299739 1.4387456045858977 6 0.4523857876526487 1.0872469626396577 7 0.3493071476311672 0.9794302486920015 8 0.26049387576422406 0.834748225787465 9 0.20019338988165292 0.7217058792411299 >10 1.593128580804556 12.689058986290517 >50 0.18701875027294154 5.158128266524467 >100 0.14961500021835325 11.751243322057839 >500 0.01593122687510243 4.3537920907052845 >1k 0.01177525464681484 8.620186494144946 >5k 6.926620380479317E-4 1.9224716668682713 >10k+ 0.0013853240760958634 6.313442456283376 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGC 11433 2.7972010862916843 No Hit GAGTAAGCAGTGGTATCAACGCAGA 11322 2.7700437941917646 No Hit CTGCTTACTCTGCGTTGATACCACT 6929 1.6952511437868518 No Hit GCGTTGATACCACTGCTTACTCTGC 4043 0.9891615491889512 No Hit GNGTAAGCAGTGGTATCAACGCAGA 2610 0.6385633547818853 No Hit GNTTACTCTGCGTTGATACCACTGC 2567 0.6280429623467815 No Hit GTATCAACGCAGAGTAAGCAGTGGT 2457 0.6011303305360507 No Hit GTACATGGGAAGCAGTGGTATCAAC 1867 0.4567807599148582 No Hit GAGTACATGGGAAGCAGTGGTATCA 1788 0.4374525970689698 No Hit TGCTTACTCTGCGTTGATACCACTG 1756 0.42962346781493893 No Hit ACGCAGAGTAAGCAGTGGTATCAAC 1714 0.41934773566902356 No Hit CCACTGCTTACTCTGCGTTGATACC 1620 0.39634966848530817 No Hit ACTCTGCGTTGATACCACTGCTTAC 1506 0.3684583955178235 No Hit CNGCTTACTCTGCGTTGATACCACT 1449 0.3545127590340812 No Hit AAGCAGTGGTATCAACGCAGAGTAA 1392 0.34056712255033883 No Hit GTAAGCAGTGGTATCAACGCAGAGT 1352 0.3307807109828004 No Hit TATCAACGCAGAGTAAGCAGTGGTA 1318 0.32246226115039267 No Hit GTGGTATCAACGCAGAGTAAGCAGT 1313 0.3212389597044504 No Hit GGTATCAACGCAGAGTAAGCAGTGG 1287 0.31487779218555034 No Hit CAGTGGTATCAACGCAGAGTAAGCA 1030 0.2520000978641157 No Hit GCAGTGGTATCAACGCAGAGTAAGC 927 0.22680008807770413 No Hit GNGTTGATACCACTGCTTACTCTGC 914 0.22361950431825411 No Hit GATACCACTGCTTACTCTGCGTTGA 882 0.21579037506422333 No Hit TACCACTGCTTACTCTGCGTTGATA 866 0.21187581043720793 No Hit GTTGATACCACTGCTTACTCTGCGT 822 0.20111075771291564 No Hit GAGCAAGCAGTGGTATCAACGCAGA 817 0.19988745626697332 No Hit ACTGCTTACTCTGCGTTGATACCAC 786 0.192302987302131 No Hit ATACCACTGCTTACTCTGCGTTGAT 750 0.18349521689134637 No Hit CCCATGTACTCTGCGTTGATACCAC 708 0.17321948474543097 No Hit GCTNACTCTGCGTTGATACCACTGC 685 0.16759229809409634 No Hit GCTTACTGCTTACTCTGCGTTGATA 650 0.15902918797250018 No Hit GCTTTCTCTGCGTTGATACCACTGC 640 0.15658258508061557 No Hit GCAGTAAGCAGTGGTATCAACGCAG 625 0.15291268074278863 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 601 0.14704083380226554 No Hit GAGAAAGCAGTGGTATCAACGCAGA 595 0.1455728720671348 No Hit GAGTAAGCAGTAAGCAGTGGTATCA 594 0.14532821177794633 No Hit GNATCAACGCAGAGTAAGCAGTGGT 592 0.1448388911995694 No Hit GCTTACTCTGCGTTTATACCACTGC 586 0.1433709294644386 No Hit GTATCAACGCAGAGTACATGGGAAG 559 0.13676510165635014 No Hit GAATAAGCAGTGGTATCAACGCAGA 552 0.13505247963203093 No Hit GAGTAAGCAGTGGTATCAACCCAGA 518 0.12673402979962323 No Hit GAGNAAGCAGTGGTATCAACGCAGA 516 0.1262447092212463 No Hit GCTTCCCATGTACTCTGCGTTGATA 507 0.12404276661855014 No Hit CACTGCTTACTCTGCGTTGATACCA 484 0.11841557996721552 No Hit CTCTGCGTTGATACCACTGCTTACT 480 0.11743693881046167 No Hit AGAGTAAGCAGTGGTATCAACGCAG 474 0.11596897707533091 No Hit GCTTATTCTGCGTTGATACCACTGC 460 0.11254373302669245 No Hit GCTTGCTCTGCGTTGATACCACTGC 452 0.11058645071318474 No Hit GCTTCCTCTGCGTTGATACCACTGC 444 0.10862916839967705 No Hit NNTNNNNCTGCNNTGATACCACTNC 442 0.10813984782130012 No Hit GTGGTAAGCAGTGGTATCAACGCAG 442 0.10813984782130012 No Hit TGATACCACTGCTTACTCTGCGTTG 440 0.10765052724292319 No Hit GAGTAAGCAGTGCTATCAACGCAGA 436 0.10667188608616934 No Hit GNACATGGGAAGCAGTGGTATCAAC 423 0.10349130232671934 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 421 0.10300198174834244 No Hit NNGNNNNCAGTNNTATCAACGCANA 418 0.10226800088077705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGATAA 30 0.0023083945 22.416613 1 CTGCTAC 55 4.0360646E-7 22.009039 1 CTTAGTA 45 3.856345E-5 21.474604 2 GTGTAAG 145 0.0 21.33443 1 TGCTACT 55 7.923829E-6 20.080149 2 AGTACGT 35 0.0048502395 19.721575 2 AGTACGC 60 1.678641E-5 18.406803 2 CTTAATA 45 9.5227495E-4 18.406803 2 AGGGCTA 30 9.6564204E-4 18.372543 9 GGCTATC 30 9.6564204E-4 18.372543 11 GGGCTAT 30 9.6564204E-4 18.372543 10 GTACGTG 30 0.007408656 18.328878 3 TTAGTAC 50 2.4771805E-5 17.595722 3 GAGTACG 100 1.4570105E-9 17.484957 1 GAGAAAG 255 0.0 17.40584 1 GAGTAGC 85 7.655035E-8 17.40584 1 GCAGTGC 260 0.0 17.280191 7 GAGAAAA 50 0.0022964417 16.139961 1 GTGCTAT 275 0.0 16.03422 10 GCTACTC 55 5.6082506E-5 15.996112 3 >>END_MODULE