FastQCFastQC Report
Thu 26 May 2016
SRR1926491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408730
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA274646.719350182271916No Hit
GCTTACTCTGCGTTGATACCACTGC265636.498911261713111No Hit
CTGCTTACTCTGCGTTGATACCACT178414.3649842194113475No Hit
GCGTTGATACCACTGCTTACTCTGC96882.370268881657818No Hit
GTATCAACGCAGAGTAAGCAGTGGT55531.3585985858635286No Hit
CCACTGCTTACTCTGCGTTGATACC46581.139627627039855No Hit
ACGCAGAGTAAGCAGTGGTATCAAC46421.1357130624128398No Hit
GTACATGGGAAGCAGTGGTATCAAC46271.132043158075013No Hit
ACTCTGCGTTGATACCACTGCTTAC39470.9656741614268587No Hit
GAGTACATGGGAAGCAGTGGTATCA39260.9605362953539011No Hit
AAGCAGTGGTATCAACGCAGAGTAA35810.8761284955838817No Hit
TATCAACGCAGAGTAAGCAGTGGTA32520.7956352604408778No Hit
GTGGTATCAACGCAGAGTAAGCAGT32050.7841362268490202No Hit
GTAAGCAGTGGTATCAACGCAGAGT31960.7819342842463239No Hit
GGTATCAACGCAGAGTAAGCAGTGG30040.7349595087221393No Hit
TGCTTACTCTGCGTTGATACCACTG27730.6784429819196046No Hit
CAGTGGTATCAACGCAGAGTAAGCA25630.6270643211900276No Hit
GCAGTGGTATCAACGCAGAGTAAGC24650.6030876128495584No Hit
GAGCAAGCAGTGGTATCAACGCAGA22790.5575807990605044No Hit
GATACCACTGCTTACTCTGCGTTGA22510.5507303109632276No Hit
CCCATGTACTCTGCGTTGATACCAC22410.548283708071343No Hit
ACTGCTTACTCTGCGTTGATACCAC21000.5137866072957699No Hit
TACCACTGCTTACTCTGCGTTGATA20740.5074254397768698No Hit
ATACCACTGCTTACTCTGCGTTGAT20720.5069361191984929No Hit
GTTGATACCACTGCTTACTCTGCGT19170.4690137743742813No Hit
GCAGTAAGCAGTGGTATCAACGCAG17370.4249749223203582No Hit
GCTTACTGCTTACTCTGCGTTGATA15690.3838719937366966No Hit
GAATAAGCAGTGGTATCAACGCAGA14370.35157683556381963No Hit
GTATCAACGCAGAGTACATGGGAAG13480.32980206982604654No Hit
GAGTAAGCAGTAAGCAGTGGTATCA13170.3222176008612042No Hit
GCTTCCCATGTACTCTGCGTTGATA12750.3119418687152888No Hit
GTGGTAAGCAGTGGTATCAACGCAG12200.29848555280992345No Hit
CTCTGCGTTGATACCACTGCTTACT12110.29628361020722727No Hit
CACTGCTTACTCTGCGTTGATACCA11780.288209820664008No Hit
GCTTGCTCTGCGTTGATACCACTGC11360.27793408851809265No Hit
GCTTTCTCTGCGTTGATACCACTGC11210.2742641841802657No Hit
GCTTCCTCTGCGTTGATACCACTGC10970.2683923372397426No Hit
AGAGTAAGCAGTGGTATCAACGCAG10940.2676583563721772No Hit
GAGAAAGCAGTGGTATCAACGCAGA10870.265945734347858No Hit
AAAGTACTCTGCGTTGATACCACTG9930.2429476671641426No Hit
GCTTATTCTGCGTTGATACCACTGC9570.23413989675335795No Hit
AAAAAGTACTCTGCGTTGATACCAC9250.22631076749932716No Hit
ACGCAGAGTACATGGGAAGCAGTGG8900.21774765737773102No Hit
CAGAGTAAGCAGTGGTATCAACGCA8690.21260979130477334No Hit
GCTTAGTACTCTGCGTTGATACCAC8480.20747192523181565No Hit
TGATACCACTGCTTACTCTGCGTTG8340.20404668118317715No Hit
GTACTAAGCAGTGGTATCAACGCAG8270.2023340591588579No Hit
CATGTACTCTGCGTTGATACCACTG8170.19988745626697332No Hit
GCTTACCACTGCTTACTCTGCGTTG8060.19719619308590022No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA7650.1871651212291733No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7120.1741981259021848No Hit
GGTATCAACGCAGAGTACATGGGAA6760.1653903554914002No Hit
CATGGGAAGCAGTGGTATCAACGCA6720.16441171433464635No Hit
TATCAACGCAGAGTACATGGGAAGC6710.1641670540454579No Hit
CAGAATAAGCAGTGGTATCAACGCA6660.16294375259951557No Hit
ATCAACGCAGAGTAAGCAGTGGTAT6580.16098647028600788No Hit
GCGTTGATACCACTGCTTGCTCTGC6540.16000782912925401No Hit
AGTAAGCAGTGGTATCAACGCAGAG6510.15927384826168864No Hit
AACGCAGAGTAAGCAGTGGTATCAA6380.15609326450223862No Hit
CTGCTTGCTCTGCGTTGATACCACT6220.15217869987522326No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6200.15168937929684634No Hit
AAGCAGTGGTATCAACGCAGAGTAC6040.14777481466983095No Hit
GCGTTGATACCACTGCTTCCCATGT5820.14239228830768477No Hit
GGTATAAGCAGTGGTATCAACGCAG5820.14239228830768477No Hit
CTGTTTACTCTGCGTTGATACCACT5680.13896704425904632No Hit
GAGGAAGCAGTGGTATCAACGCAGA5400.13211655616176937No Hit
GACTAAGCAGTGGTATCAACGCAGA5110.125021407775304No Hit
CAGAGCAAGCAGTGGTATCAACGCA5080.1242874269077386No Hit
CTGCCTACTCTGCGTTGATACCACT4930.12061752256991168No Hit
GAGTAGGCAGTGGTATCAACGCAGA4900.11988354170234629No Hit
GGGTAAGCAGTGGTATCAACGCAGA4870.11914956083478091No Hit
GTACTCTGCGTTGATACCACTGCTT4850.11866024025640398No Hit
AGAGCAAGCAGTGGTATCAACGCAG4850.11866024025640398No Hit
GCGTTGATACCACTGCTTACTGCTT4820.11792625938883859No Hit
GATTAAGCAGTGGTATCAACGCAGA4580.11205441244831552No Hit
CGTTGATACCACTGCTTACTCTGCG4560.11156509186993858No Hit
CAACGCAGAGTAAGCAGTGGTATCA4520.11058645071318474No Hit
GTACATGGTAAGCAGTGGTATCAAC4480.1096078095564309No Hit
AGCTTACTCTGCGTTGATACCACTG4450.10887382868886551No Hit
CAGACTAAGCAGTGGTATCAACGCA4400.10765052724292319No Hit
CTGCTACTCTGCGTTGATACCACTG4380.10716120666454627No Hit
GAGTACGCAGTGGTATCAACGCAGA4350.1064272257969809No Hit
CTTCTTACTCTGCGTTGATACCACT4270.10446994348347319No Hit
GCTTATACCACTGCTTACTCTGCGT4220.1032466420375309No Hit
GTATCAACGCAGAGTACTTTTTTTT4170.10202334059158857No Hit
GTACCAACGCAGAGTAAGCAGTGGT4150.10153402001321166No Hit
GAGTAAGCAGTGGTAAGCAGTGGTA4130.10104469943483473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAT307.461169E-419.0926362
TTACATG401.2313678E-519.0879273
AATCCGT250.005874378619.0832215
TGGTACT250.005874378619.0832214
TAATCCG250.005874378619.0832214
GGTACTC401.2339908E-519.083225
GCTTAAC307.4859755E-419.083221
GTACATA453.3986747E-516.9628621
CTTTACT350.002097218816.3651162
GAGAAAA702.3566827E-816.3570461
TACATGT350.002104144316.3570464
CGTGGTA651.888493E-716.0878359
CCGTGGT551.117025E-515.5560078
GATTAAG1600.015.5051161
CTTCCAT508.335535E-515.2741082
GAGTGAG1250.015.2665761
GCTTAAA902.1318556E-914.84250451
CTTAGCA400.005102841614.3194772
TACATAG400.005102841614.3194772
AGTGAGC1203.6379788E-1214.3194772
Produced by FastQC (version 0.11.5)