FastQCFastQC Report
Thu 26 May 2016
SRR1926490_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926490_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693481
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT8950.12905905136550244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC200.00912178326.6780911
AATTGTC500.002049672716.4210412
TGTAGGA4700.015.1399662
GTCCTAG1109.907308E-814.5516851
TAGGACC2300.014.4618194
TCCAGAA1652.3646862E-1114.0988732
CCTATAC702.8487055E-514.0899083
TATACTC450.006167365713.9169995
TCTACAC806.5729128E-613.6985233
GTAGGAC5450.013.6733873
GTCCTAC6100.013.5577161
TCACGTC801.4500984E-613.437088
GTCCTAA2600.013.3390461
CTATACT1151.2883902E-813.0622884
GGCGAGG2150.013.03381419
ATACTGC653.643857E-412.8387816
GACTGTG801.3822828E-512.7460697
CTGTAGG5050.012.6786961
AGGACGT5200.012.6457355
GTCCAGA957.908567E-512.6369891
Produced by FastQC (version 0.11.5)