FastQCFastQC Report
Thu 26 May 2016
SRR1926490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693481
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA19040.2745569092736499No Hit
GTCCTACAGTGGACATTTCTAAATT17660.2546573013536059No Hit
GTCCTAAAGTGTGTATTTCTCATTT13280.19149767621607514No Hit
CTTTAGGACGTGAAATATGGCGAGG13180.19005567564215892No Hit
CCCATGTACTCTGCGTTGATACCAC12580.18140367219866155No Hit
GTACATGGGAAGCAGTGGTATCAAC12500.18025007173952856No Hit
GTCCTACAGTGTGCATTTCTCATTT12230.17635667018995474No Hit
CTGTAGGACCTGGAATATGGCGAGA10200.14708405853945528No Hit
GTATCAACGCAGAGTACTTTTTTTT8640.12458884958636213No Hit
CTGAAGGACCTGGAATATGGCGAGA7460.10757324281415065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTAT508.4004874E-515.2631131
GTATTAC456.5466185E-414.8391361
TAGGACT1006.148184E-1014.3122874
GTCTAAG602.4659012E-514.3091681
TAGGACC3800.014.0611934
CTAGGAC1051.859189E-812.7266563
TCCAACG604.1975765E-412.62661918
TGTAGGA9100.012.5877172
GTAGGAC8550.012.2801073
GATATAC2800.012.2650011
CTATTAC701.0478691E-412.2650011
CTGTAGG8900.012.2190651
TACACTG3850.012.1437595
AGCTAGC550.002966934112.1437586
TATACAC3000.012.0903233
TATACTG1302.4301698E-911.7434155
AGGACGT7850.011.5470685
ATAGGAC1001.8179162E-611.4539913
CTACACT1501.6189006E-1011.4498294
AACTTGC752.0676391E-411.401787
Produced by FastQC (version 0.11.5)