FastQCFastQC Report
Thu 26 May 2016
SRR1926489_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926489_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212924
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17400.14345498976028176No Hit
CTGTAGGACGTGGAATATGGCAAGA13170.10858058707717878No Hit
GTCCTAAAGTGTGTATTTCTCATTT12290.10132539219275075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACC350.001809142116.7342018
TAGGACC4500.015.7306144
TGTAGGA11050.015.3785332
CTGTAGG11150.015.0969581
CTAGGAC1750.015.0194023
GAGGTCG450.00619480313.9093416
GTAGGAC11950.013.4719013
GTGTAGA1101.5493606E-613.359611
GGCTAGG600.00808704813.359611
GTCCTAT1305.1524694E-813.3596091
AGGACGG550.001517587313.2778515
GTCCTAC10800.013.112211
GTCCTAA4900.013.0869631
ATAGGAC851.2393084E-512.8842913
TTTAGGA6100.012.8056182
TCCTAGG1409.730138E-812.7253822
TCCTACA11900.012.6678022
AGGACCT7200.012.4611595
TAGGACG11750.012.3228214
TTCTAGG1451.5373371E-712.2865752
Produced by FastQC (version 0.11.5)