FastQCFastQC Report
Thu 26 May 2016
SRR1926489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212924
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA35730.29457740138706134No Hit
GTCCTACAGTGGACATTTCTAAATT34060.28080902018593085No Hit
CTTTAGGACGTGAAATATGGCGAGG26890.2216956709571251No Hit
GTCCTAAAGTGTGTATTTCTCATTT26590.21922230906470647No Hit
GTCCTACAGTGTGCATTTCTCATTT23170.19102598349113384No Hit
CTGTAGGACCTGGAATATGGCGAGA19300.1591196150789332No Hit
CCCATGTACTCTGCGTTGATACCAC17210.14188852722841663No Hit
GTACATGGGAAGCAGTGGTATCAAC16560.13652957646150954No Hit
CTGAAGGACCTGGAATATGGCGAGA15230.12556433873845352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7300.014.500834
TGTAGGA19050.012.6200332
CTGTAGG18500.012.4722911
GTAGGAC17850.012.2885433
ATAGGAC2100.012.2618273
GTCTTAG1251.3296813E-912.2042921
GTCGAGG701.1191293E-412.18044319
ATACGGC550.003131216612.05790515
CTAGGAC2100.011.8076863
TTGCGAG658.210054E-411.65991118
GGACGTG17500.011.5528876
AGGACGT17650.011.5087365
GGCGAGG7400.011.39401819
TAGGACG18550.011.25882154
GACGTGG8950.011.2526947
TCGAACT855.4728756E-511.14550319
GGACCTG12450.011.1068626
AGGACCT12750.010.9951175
GATATAC2000.010.9647921
CTAATAC700.00144343210.8994033
Produced by FastQC (version 0.11.5)