FastQCFastQC Report
Thu 26 May 2016
SRR1926488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176785
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19190.16307141916322862No Hit
CTGTAGGACGTGGAATATGGCAAGA14730.1251715479038227No Hit
GTCCTAAAGTGTGTATTTCTCATTT13950.11854331929791763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA10750.015.1174022
TAGGACC4900.014.9634084
GTCCTAC12350.014.55929851
GTCCTAA6250.014.3845871
TGCCGGT458.6092664E-414.31317810
CTGTAGG10800.014.2881741
TTTAGGA6250.013.8823872
GCGTGCG400.006521556513.8019939
GTAGGAC11700.013.75488953
ACGTGTA500.001197545313.7015258
TCCTACA13750.013.5218062
GTCGTCA700.001401101613.4184591
GCCTTAC600.00785497613.4184581
CTTTAGG6500.013.2120221
GATATAC3100.012.9856041
TCCTAAA6700.012.9499882
CTTACAC952.5887111E-612.7631883
TTAGGAC6750.012.4108053
CCAACGA1101.1368684E-912.325870519
AGGACCT8400.011.98469455
Produced by FastQC (version 0.11.5)