##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1176785 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.531047727494826 27.0 7.0 31.0 2.0 31.0 2 16.498414748658423 18.0 2.0 31.0 2.0 31.0 3 20.2338099143004 25.0 15.0 31.0 2.0 31.0 4 22.272342866368962 28.0 7.0 35.0 2.0 37.0 5 23.108157394936203 30.0 2.0 35.0 2.0 37.0 6 23.557033782721568 30.0 2.0 35.0 2.0 37.0 7 23.528104114175488 31.0 7.0 35.0 2.0 37.0 8 23.680990155380975 31.0 10.0 35.0 2.0 37.0 9 26.593386217533364 32.0 17.0 37.0 2.0 39.0 10 27.370293639024972 32.0 25.0 38.0 2.0 39.0 11 24.407578274706086 30.0 8.0 37.0 2.0 39.0 12 25.6079003386345 31.0 17.0 37.0 2.0 39.0 13 22.13641149402822 28.0 2.0 37.0 2.0 39.0 14 25.62353615996125 31.0 17.0 38.0 2.0 40.0 15 26.763313604439215 31.0 17.0 38.0 2.0 40.0 16 27.135959414846383 32.0 18.0 39.0 2.0 40.0 17 26.47252726708787 31.0 17.0 38.0 2.0 40.0 18 26.58730779199259 31.0 17.0 38.0 2.0 40.0 19 26.988415046078934 31.0 19.0 39.0 2.0 40.0 20 27.246598146645308 31.0 22.0 39.0 2.0 40.0 21 25.104344463942013 30.0 8.0 38.0 2.0 40.0 22 26.03565392148948 31.0 10.0 38.0 2.0 40.0 23 26.050004886194163 31.0 2.0 39.0 2.0 40.0 24 25.035922449725312 31.0 2.0 39.0 2.0 40.0 25 24.845865642407066 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.823823773582902 1101 2 10.68817461607501 1101 3 7.315406466732853 1101 4 8.931431403063904 1101 5 8.517194300227509 1101 6 7.930124630093534 1101 7 7.5279507056199435 1101 8 5.900129069960119 1101 9 6.0062017267253935 1101 10 5.597771701932945 1101 11 8.336549880707402 1101 12 6.1373603059728445 1101 13 10.73163135262606 1101 14 8.132897023599735 1101 15 7.1563983859241205 1101 16 6.750663511945763 1101 17 2.6531612006574967 1101 18 5.179855456589934 1101 19 5.760050815220119 1101 20 3.85253678371361 1101 21 1.2802314402408221 1101 22 5.132923450011237 1101 23 6.323097343532179 1101 24 7.5807862321118265 1101 25 8.065744559878759 1102 1 6.1898999170119104 1102 2 11.315792159364133 1102 3 7.449003057803253 1102 4 8.739345395212627 1102 5 8.764923444149481 1102 6 7.294458884828945 1102 7 7.427947866565919 1102 8 7.643969742248114 1102 9 6.791083764030567 1102 10 5.801896073147002 1102 11 8.6109207076206 1102 12 6.037675182874043 1102 13 10.648538951741308 1102 14 8.179454670591763 1102 15 6.0605723115357435 1102 16 6.563812461624824 1102 17 7.159341563178888 1102 18 7.305016443858065 1102 19 6.243670763962292 1102 20 6.408193340766978 1102 21 5.437325332919418 1102 22 6.534054683993162 1102 23 7.130688715905091 1102 24 8.433705941082977 1102 25 8.249869602141949 1103 1 5.007754279215558 1103 2 9.366992481271765 1103 3 6.419453202176857 1103 4 7.93140882678016 1103 5 7.282546046338176 1103 6 6.665168349447498 1103 7 6.8633069416207455 1103 8 6.1925855471973925 1103 9 5.286638996364857 1103 10 4.362617637821369 1103 11 5.360073353700841 1103 12 4.4234528078563145 1103 13 8.469865176972426 1103 14 6.586154476944198 1103 15 5.671053184399078 1103 16 5.510413803027628 1103 17 3.6833761319520306 1103 18 2.1963868769425794 1103 19 4.043504935899236 1103 20 4.46975817588395 1103 21 6.595737542179084 1103 22 2.43790240840746 1103 23 4.71387277546215 1103 24 6.048657897353735 1103 25 1.7909059549408965 1104 1 6.0950189901721785 1104 2 10.92002119072039 1104 3 7.384534384804223 1104 4 7.879916868365093 1104 5 8.129145193936562 1104 6 7.412083792996658 1104 7 7.5368458140736045 1104 8 7.328952936126395 1104 9 6.3810086728484166 1104 10 5.168539464797899 1104 11 8.410473931340313 1104 12 5.47318245469112 1104 13 9.898417180499607 1104 14 7.58479830214436 1104 15 6.734230584893652 1104 16 6.252153239062363 1104 17 6.709410803100088 1104 18 4.125634987154147 1104 19 5.173487619494658 1104 20 4.195250351552804 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6.387288000394399 2204 5 1.5282649126998429 2204 6 4.436732440253181 2204 7 5.559440300082592 2204 8 5.40332511120398 2204 9 4.121049107065549 2204 10 3.5379681061309434 2204 11 5.128309799323883 2204 12 5.2268811553877725 2204 13 3.5677672712834365 2204 14 4.6697271559501985 2204 15 4.896352479893459 2204 16 4.74074872948821 2204 17 -0.5406593773086321 2204 18 3.2753098393889744 2204 19 3.9589624640032 2204 20 4.32892278288546 2204 21 6.028305482885379 2204 22 5.310832322961954 2204 23 3.913129395961164 2204 24 5.673670286156206 2204 25 5.762482062243073 2205 1 5.956840728435907 2205 2 10.41941892818675 2205 3 7.247553421981539 2205 4 7.7227086554676525 2205 5 7.912327940871677 2205 6 7.281879467271548 2205 7 7.465160376019185 2205 8 6.96066254441903 2205 9 5.826141484569732 2205 10 5.162857465395927 2205 11 7.3922585421247256 2205 12 6.589871879588959 2205 13 9.912868740352092 2205 14 7.353756240946314 2205 15 6.185526309004089 2205 16 5.866491173877474 2205 17 6.548013414482529 2205 18 6.297886556973744 2205 19 4.977026441382428 2205 20 5.297415705883516 2205 21 -1.221613413951399 2205 22 3.5029552032656994 2205 23 4.9405898256277005 2205 24 6.784370096314728 2205 25 7.127813764167922 2206 1 4.082038643408925 2206 2 8.966035404777617 2206 3 6.0600732618688085 2206 4 2.011621932900894 2206 5 5.468242066097172 2206 6 5.8438495971115145 2206 7 5.89668276315442 2206 8 5.891515698283644 2206 9 4.567928255139343 2206 10 3.6655619807607103 2206 11 3.6715547971282483 2206 12 4.541819672619255 2206 13 7.944162049374306 2206 14 0.21201137608981924 2206 15 2.397282748741155 2206 16 3.2650372488703105 2206 17 4.286196584064204 2206 18 -1.0006834226637586 2206 19 0.9020810252674067 2206 20 2.2518875196943995 2206 21 3.5781788963018535 2206 22 -1.8630989431739629 2206 23 -2.450012552061814 2206 24 1.418568061613314 2206 25 2.8980417389896544 2207 1 1.4260127770449884 2207 2 -14.171624378899864 2207 3 -6.504712453773591 2207 4 -4.916826976501085 2207 5 0.967434107485694 2207 6 1.7792508953972686 2207 7 2.929421158428813 2207 8 3.08309466564533 2207 9 0.5330939889279378 2207 10 0.4081489970265366 2207 11 -1.5285318429181523 2207 12 -2.307949701220128 2207 13 0.3575300387261997 2207 14 -5.667289396365366 2207 15 -1.7023547656684137 2207 16 -0.5451867149191187 2207 17 0.6312131742450973 2207 18 0.6299290041195569 2207 19 0.16145108053878943 2207 20 -0.04932617147839835 2207 21 -3.533180592682587 2207 22 -4.4828784420642975 2207 23 -1.5741927224490375 2207 24 0.3573115033416734 2207 25 0.9043446927869851 2208 1 4.809484733330763 2208 2 6.882029645151938 2208 3 6.397066850055573 2208 4 8.344921610809383 2208 5 3.860712074110804 2208 6 6.381357203692332 2208 7 6.872919304395506 2208 8 6.849698378110492 2208 9 5.2306877089027 2208 10 4.669593809696554 2208 11 6.492054043519616 2208 12 6.140758890012378 2208 13 8.86859879855134 2208 14 3.244557081521169 2208 15 4.6838236714637524 2208 16 5.276193567403443 2208 17 5.936171069613746 2208 18 0.7400988567544928 2208 19 3.424975810222886 2208 20 4.492012608722092 2208 21 6.977064355975834 2208 22 6.008959504474117 2208 23 1.9324768032730688 2208 24 3.525957974263843 2208 25 4.9066484316892485 2209 1 3.2008542461427467 2209 2 4.881084980706046 2209 3 5.30141285334572 2209 4 0.6844576178818329 2209 5 5.136599206532434 2209 6 5.645402992348156 2209 7 6.128354200929003 2209 8 4.794970271039407 2209 9 4.415897991299495 2209 10 3.4384727440895375 2209 11 -0.5629575084551597 2209 12 3.104028622927075 2209 13 2.321907858307174 2209 14 3.5174552828764156 2209 15 3.899953600420247 2209 16 3.609031707603858 2209 17 1.080789489886893 2209 18 3.150739433122844 2209 19 3.8112328336868373 2209 20 3.933673996873001 2209 21 6.184900103755439 2209 22 5.006676451471741 2209 23 0.7068387104653695 2209 24 -2.987038111280011 2209 25 0.38365430565196235 2210 1 -2.2919826246634756 2210 2 -14.171624378899864 2210 3 -7.944975173981003 2210 4 -2.4154511578890094 2210 5 -0.6561342768140754 2210 6 -1.4805067159370324 2210 7 -1.3432385741120498 2210 8 -21.36167997526601 2210 9 -13.506651117743866 2210 10 -7.621115429195953 2210 11 -10.457483041103297 2210 12 -5.177370756748168 2210 13 -5.602885366773407 2210 14 -4.3676437460923445 2210 15 -6.071343202677323 2210 16 -5.5806690769245115 2210 17 -5.055608470744783 2210 18 -5.1955193598792455 2210 19 -10.678675739363499 2210 20 -7.497548002356659 2210 21 -5.459377096268888 2210 22 -10.128535594180917 2210 23 -8.057686720822513 2210 24 -10.789797137410686 2210 25 -8.330171233077792 2211 1 -0.2133318474008803 2211 2 4.673281855261312 2211 3 0.5450050424803194 2211 4 1.4007809383734973 2211 5 0.9865726160336052 2211 6 -0.1022531737762975 2211 7 -21.176571907445382 2211 8 -13.523362691941125 2211 9 -9.313205496924532 2211 10 -7.088677778107911 2211 11 -9.771508913007752 2211 12 -4.806845517875235 2211 13 -7.10395458517241 2211 14 -4.701683634378444 2211 15 -5.131261148443105 2211 16 -11.92619009211101 2211 17 -7.810174484356526 2211 18 -5.905816185961395 2211 19 -8.84383022199841 2211 20 -6.70621949132606 2211 21 -4.577892173265127 2211 22 -6.128901386283772 2211 23 -8.40060912596686 2211 24 -22.736299195023854 2211 25 -22.55033584213129 2212 1 -1.6333410549956717 2212 2 -14.171624378899864 2212 3 -8.189853197779952 2212 4 -19.952488194926048 2212 5 -20.723006704797616 2212 6 -21.194498485484356 2212 7 -10.393920354956471 2212 8 -10.075824874231017 2212 9 -5.996279134951152 2212 10 -6.057079191271374 2212 11 -6.275870030578943 2212 12 -3.5887346835137706 2212 13 -6.810628492682294 2212 14 -4.576769346694313 2212 15 -4.638558904223018 2212 16 -4.695344249879625 2212 17 -4.852527172207321 2212 18 -3.9421642030686357 2212 19 -4.588691417382023 2212 20 -4.7331873024639535 2212 21 -2.5440216636225053 2212 22 -3.8524754518415456 2212 23 -3.9646699800249827 2212 24 -2.9891331033530797 2212 25 -3.4473294350341988 2213 1 -19.216879744005038 2213 2 -14.171624378899864 2213 3 -8.863054591904366 2213 4 -5.991546514883243 2213 5 -20.723006704797616 2213 6 -11.002952204050434 2213 7 -21.176571907445382 2213 8 -10.981262639792496 2213 9 -8.9214426481721 2213 10 -7.556955384542775 2213 11 -6.9409247046323514 2213 12 -5.399607841344803 2213 13 -19.761835984057356 2213 14 -12.87541016241057 2213 15 -7.5785597111422724 2213 16 -6.631305186549383 2213 17 -5.288723987724222 2213 18 -5.34564255244938 2213 19 -6.147491175095286 2213 20 -6.337547936301661 2213 21 -4.440645550383781 2213 22 -5.267423657382338 2213 23 -5.025677294774386 2213 24 -4.0359246631886485 2213 25 -4.929148041168208 2214 1 -19.216879744005038 2214 2 -14.171624378899864 2214 3 -12.76001955479034 2214 4 -19.952488194926048 2214 5 -20.723006704797616 2214 6 -21.194498485484356 2214 7 -17.031034670378865 2214 8 -15.532231424953334 2214 9 -14.629754346991685 2214 10 -14.60967485432761 2214 11 -15.311886731003796 2214 12 -13.229194183358404 2214 13 -19.761835984057356 2214 14 -17.47757632095133 2214 15 -24.45817447839749 2214 16 -18.96905789211833 2214 17 -24.219703120950545 2214 18 -18.607589442721864 2214 19 -15.803291764649094 2214 20 -14.538575685968514 2214 21 -12.07387993528376 2214 22 -13.200490350086465 2214 23 -13.066951536456163 2214 24 -11.89832307222681 2214 25 -11.707243175843626 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 201772.0 3 9.0 4 792.0 5 3463.0 6 4768.0 7 4748.0 8 4050.0 9 3677.0 10 3441.0 11 3340.0 12 3056.0 13 3457.0 14 5386.0 15 12256.0 16 18459.0 17 19177.0 18 19735.0 19 22765.0 20 26941.0 21 31116.0 22 32123.0 23 34611.0 24 36499.0 25 37684.0 26 30806.0 27 23477.0 28 24861.0 29 29243.0 30 28494.0 31 32864.0 32 43081.0 33 54941.0 34 68113.0 35 79783.0 36 78834.0 37 83731.0 38 65232.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08241145530084 18.658285060148224 12.106421402701981 26.152882081848954 2 13.347648665993322 20.574815665270336 42.30898372871994 23.76855194001641 3 19.01466224787403 24.188823875936492 29.374369798010097 27.42214407817938 4 11.572957558919523 16.742101623172665 38.91621369362442 32.76872712428339 5 12.011011588121432 37.7737294969103 37.439696794989906 12.775562119978364 6 31.20104044707842 37.62688746997549 18.83858988440818 12.333482198537903 7 27.442427979748036 30.73036422328299 22.65486492736403 19.172342869604943 8 23.715277425097668 38.92104818477216 21.134982552722725 16.228691837407442 9 26.14105130445361 15.232166115257433 20.389834560215334 38.23694802007362 10 13.816671399802678 29.759714941488944 35.76015171355348 20.663461945154896 11 33.201793900269195 21.814611630351 26.09441742067648 18.889177048703328 12 23.858942548358627 24.37752282295751 32.809629045559106 18.953905583124754 13 28.534871066853768 21.783217212360235 25.81352867294797 23.868383047838023 14 19.376578738530643 23.17756734654359 28.034938423794365 29.4109154911314 15 21.649509861982256 33.4408101205407 23.264995643900015 21.644684373577032 16 20.586852359673177 28.28090102513628 29.999403480515745 21.1328431346748 17 20.507868176360123 29.123786618530495 29.845916889673617 20.522428315435764 18 20.287211272861075 27.600682010167965 32.44894856765772 19.66315814931324 19 22.83508069215264 26.84176223546829 27.51026186642956 22.81289520594951 20 22.80397819508699 28.32653172526219 28.977832921600992 19.891657158049828 21 21.297816033574808 26.711130120202803 27.056323344649684 24.934730501572705 22 21.80849178221597 29.655007837783394 28.15731248330202 20.37918789669862 23 20.815776511332924 28.93508888792474 29.082094040761604 21.16704055998074 24 22.378061937687047 27.391993695527766 29.511684851441487 20.7182595153437 25 21.588915602789815 28.116380725835405 29.293408812864868 21.001294858509908 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1224.0 1 1224.0 2 2596.0 3 3968.0 4 3968.0 5 3968.0 6 5742.0 7 7516.0 8 7516.0 9 7516.0 10 8675.0 11 9834.0 12 9834.0 13 9834.0 14 12032.5 15 14231.0 16 14231.0 17 14231.0 18 20253.5 19 26276.0 20 26276.0 21 26276.0 22 37582.5 23 48889.0 24 48889.0 25 48889.0 26 65216.0 27 81543.0 28 81543.0 29 81543.0 30 99345.0 31 117147.0 32 117147.0 33 117147.0 34 128427.5 35 139708.0 36 139708.0 37 139708.0 38 144229.0 39 148750.0 40 148750.0 41 148750.0 42 148345.5 43 147941.0 44 147941.0 45 147941.0 46 143854.0 47 139767.0 48 139767.0 49 139767.0 50 126378.5 51 112990.0 52 112990.0 53 112990.0 54 95105.0 55 77220.0 56 77220.0 57 77220.0 58 63117.0 59 49014.0 60 49014.0 61 49014.0 62 38481.0 63 27948.0 64 27948.0 65 27948.0 66 20933.0 67 13918.0 68 13918.0 69 13918.0 70 9890.5 71 5863.0 72 5863.0 73 5863.0 74 3999.0 75 2135.0 76 2135.0 77 2135.0 78 1388.5 79 642.0 80 642.0 81 642.0 82 414.5 83 187.0 84 187.0 85 187.0 86 124.0 87 61.0 88 61.0 89 61.0 90 35.5 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.889962907412995 2 37.23560378488849 3 5.948155355481247 4 14.235990431557166 5 14.216955518637645 6 15.516598189133953 7 9.601329044812774 8 7.9715496033685 9 0.11149020424291607 10 1.553894721635643 11 5.960816971664323 12 6.0232752796815054 13 16.916344107037396 14 1.7001406374146508 15 3.1444996324732215 16 3.130478379653038 17 3.117476854310685 18 1.6172028025510183 19 1.561032813980464 20 0.2794053289258446 21 4.63253695449891 22 1.7172210726683295 23 0.11183011340219326 24 9.42185700871442 25 0.11616395518297734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1176785.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.33688442338317 #Duplication Level Percentage of deduplicated Percentage of total 1 95.99081442138622 86.71511108091188 2 2.960439493749788 5.348737607785872 3 0.48291623208051626 1.3087544353089984 4 0.19823946549466276 0.7163334273017842 5 0.09934178924891383 0.44871138668956073 6 0.06031691360252104 0.32693052317316784 7 0.04067024398863081 0.2571816191470215 8 0.02823364665355868 0.20404317396745353 9 0.021961712405570193 0.17855574077894174 >10 0.09956310803774214 1.656373917873176 >50 0.009358350808995611 0.5811198039070785 >100 0.007151380834093901 1.3275951885374293 >500 4.966208543975746E-4 0.29968604724938275 >1k 4.966208543975746E-4 0.6308660473683861 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1919 0.16307141916322862 No Hit CTGTAGGACGTGGAATATGGCAAGA 1473 0.1251715479038227 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1395 0.11854331929791763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 8.49772898192958E-5 0.0 0.0 0.0 8 0.0 8.49772898192958E-5 0.0 0.0 0.0 9 0.0 8.49772898192958E-5 0.0 0.0 0.0 10 0.0 8.49772898192958E-5 0.0 0.0 0.0 11 0.0 8.49772898192958E-5 0.0 0.0 0.0 12 0.0 8.49772898192958E-5 0.0 0.0 0.0 13 0.0 8.49772898192958E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 1075 0.0 15.117402 2 TAGGACC 490 0.0 14.963408 4 GTCCTAC 1235 0.0 14.5592985 1 GTCCTAA 625 0.0 14.384587 1 TGCCGGT 45 8.6092664E-4 14.313178 10 CTGTAGG 1080 0.0 14.288174 1 TTTAGGA 625 0.0 13.882387 2 GCGTGCG 40 0.0065215565 13.801993 9 GTAGGAC 1170 0.0 13.7548895 3 ACGTGTA 50 0.0011975453 13.701525 8 TCCTACA 1375 0.0 13.521806 2 GTCGTCA 70 0.0014011016 13.418459 1 GCCTTAC 60 0.007854976 13.418458 1 CTTTAGG 650 0.0 13.212022 1 GATATAC 310 0.0 12.985604 1 TCCTAAA 670 0.0 12.949988 2 CTTACAC 95 2.5887111E-6 12.763188 3 TTAGGAC 675 0.0 12.410805 3 CCAACGA 110 1.1368684E-9 12.3258705 19 AGGACCT 840 0.0 11.9846945 5 >>END_MODULE