FastQCFastQC Report
Thu 26 May 2016
SRR1926488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176785
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA41730.3546102304159214No Hit
GTCCTACAGTGGACATTTCTAAATT39670.3371049087131464No Hit
CTTTAGGACGTGAAATATGGCGAGG31300.2659789171343958No Hit
GTCCTAAAGTGTGTATTTCTCATTT30790.26164507535361176No Hit
GTCCTACAGTGTGCATTTCTCATTT25640.21788177109667442No Hit
CTGTAGGACCTGGAATATGGCGAGA23160.19680740322148907No Hit
CTGAAGGACCTGGAATATGGCGAGA18640.15839766822316736No Hit
CCCATGTACTCTGCGTTGATACCAC18100.15380889457292538No Hit
GTACATGGGAAGCAGTGGTATCAAC16570.14080736923057313No Hit
GTATCAACGCAGAGTACTTTTTTTT13660.11607897789315805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1350.014.73365918
CCAACGA851.7840648E-814.48603419
TACGGCG400.005382305514.20806116
TAGGACC8100.014.0157664
TAGTACC603.94771E-412.7201924
GATCTAC751.416381E-512.7191031
TGTAGGA23200.012.4666932
CTGTAGG22400.012.3926081
GTCCTAG2450.012.0701691
ATACGGC550.003136144712.05532515
GTAGGAC22400.012.0544973
AGGACCT14500.012.0403195
GGACCTG14100.011.9077556
GTCGAGG802.953975E-511.83954719
ACTGTTC4900.011.8279098
GCTACAC1950.011.7432233
ATCCCGT1302.7066562E-911.66582710
ACATTCG907.708428E-611.57644718
GGCGAGG8850.011.5586119
TAGGACT2600.011.3747864
Produced by FastQC (version 0.11.5)