FastQCFastQC Report
Thu 26 May 2016
SRR1926487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012848
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17260.17041056506010774No Hit
CTGTAGGACGTGGAATATGGCAAGA13620.13447229989100043No Hit
GTCCTAAAGTGTGTATTTCTCATTT13430.1325964014343712No Hit
GTCCTACAGTGTGCATTTCTCATTT11700.1155158523292735No Hit
CTTTAGGACGTGAAATATGGCGAGG10260.1012985166579783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCATAA500.002422960516.0167161
TGTAGGA11000.015.4320092
TAGGACC5550.014.6961544
GTCCTAC11850.014.6425681
GTGTAAG550.00457033614.5606511
CTGTAGG11050.014.4947661
GGGCTAC658.3302485E-414.3739761
GTACTAG658.3302485E-414.3739761
GTGTAGG1355.7225407E-913.8416071
TCTAGTA600.006879319413.6896852
ACGTGTA654.195325E-513.5243958
GTTTAGG1008.602312E-613.3472631
GTCTAAG700.001458033713.3472631
GATATAC2000.013.3472631
TCCTACA13700.013.2899862
GTCCTAA4550.013.200591
GTAGGAC12250.013.1569163
GTATAAT1254.6830246E-712.8133741
ATTTAGA2750.012.6192311
GTATTAA854.3807374E-412.562131
Produced by FastQC (version 0.11.5)