FastQCFastQC Report
Thu 26 May 2016
SRR1926487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012848
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA36460.35997504067737707No Hit
GTCCTACAGTGGACATTTCTAAATT34670.34230210258597543No Hit
CTTTAGGACGTGAAATATGGCGAGG27980.27625073061308314No Hit
GTCCTAAAGTGTGTATTTCTCATTT27310.2696357202660221No Hit
GTCCTACAGTGTGCATTTCTCATTT25470.25146912468603383No Hit
CTGTAGGACCTGGAATATGGCGAGA21880.21602451700551317No Hit
CTGAAGGACCTGGAATATGGCGAGA15430.15234270097783675No Hit
CCCATGTACTCTGCGTTGATACCAC14300.14118604173577873No Hit
GTACATGGGAAGCAGTGGTATCAAC13430.1325964014343712No Hit
GTATCAACGCAGAGTACTTTTTTTT11150.11008561995482047No Hit
GAATATGGCAAGAAAACTGAAAATC10650.10514904506895408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG402.6756088E-416.6927364
TGCACCG350.002111349516.352075
TTACACC500.001453672413.3541884
ATACCGT500.001454217413.3535256
GCCTCGA500.001531689813.26187716
TAGGACC8500.013.2419694
TGTAGGA20450.012.4079312
GGCGAGG6650.012.39233519
GTCCTAA8500.012.223481
GTAGGAC20250.012.1536233
TAAACAA959.887772E-712.0488925
CTGTAGG20500.012.0429581
TGGCGAG18700.011.95493918
TTGGACC802.747267E-511.9233834
GGACCTG13950.011.7604016
TCTAAGC657.7469245E-411.740533
TATGTCG658.214603E-411.65879316
AGGACGT16450.011.6551975
GGACGTG16100.011.5525176
AGGACCT14700.011.5502715
Produced by FastQC (version 0.11.5)