FastQCFastQC Report
Thu 26 May 2016
SRR1926486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110838
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCCG504.029456E-516.6332235
GTACTAA1055.9282684E-815.1786271
GTTCTAA550.004723963314.4886881
GTATTAG852.9043365E-514.06255151
TAGGACC2250.013.8134034
CTAGTAC500.00922012813.0973343
GTCCTAA2600.012.770481
GTCTTAA1051.4968353E-512.6488561
CGGCACA600.002384136212.5035124
CTAGCAC801.0372873E-412.2787513
TTGGACT705.425106E-412.2483384
GCTCTAT1204.3869804E-612.1745241
TTTAGTA905.9944397E-412.1031392
GTCCTAC3850.012.0739091
CTCTTAT1001.3321268E-411.9531691
CCTGGAC1751.8189894E-1111.8499693
GATTTAT907.3892344E-411.8055991
GTTATAT907.3892344E-411.8055991
ATTACTC650.003787929711.7540193
CCAATAC851.8248256E-411.5564713
Produced by FastQC (version 0.11.5)