FastQCFastQC Report
Thu 26 May 2016
SRR1926486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110838
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC22080.19876885738514524No Hit
GTACATGGGAAGCAGTGGTATCAAC20560.18508549401442873No Hit
CTTTAGGACGTGAAATATGGCGAGG11730.10559595548585841No Hit
CTGTAGGACGTGGAATATGGCAAGA11200.10082478273159544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG456.8901567E-414.74070118
TAGGACC3400.012.3382524
GCCCTAG550.002988271212.1332141
GTAGGAC7250.011.5745263
GACGTGA3050.011.5231677
AGGACGT6050.011.5034395
ATAGGAC1251.729859E-811.4429973
GGACGTG6000.011.2816
TAGGACG6450.011.0861664
TAATGCT951.30617045E-511.0394884
TAAGACA1303.0931915E-811.0008894
GTCCTAC6550.010.9159231
ATAAGTT700.001447447810.8956235
TGGCGAG7600.010.84771418
TCCTATA1158.379302E-710.7806122
TATGGCG8000.010.77913816
TGTAGGA8000.010.7287832
TTAGGAC4550.010.6885143
GACGTGG3200.010.686187
ATGGCGA8100.010.64606217
Produced by FastQC (version 0.11.5)