FastQCFastQC Report
Thu 26 May 2016
SRR1926485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729234
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCC400.002360002816.0795974
ATTAACC555.998782E-515.8754453
GTCTAAA708.6950866E-515.1988931
TAGGACC1850.014.4861244
TAGGACG1302.5465852E-1114.0181124
GTAGGAC2650.014.0033413
TGTAGGA2850.013.8605462
GAAGTGC601.9097739E-413.8575096
TCTTGGA600.00710587713.6215712
GAGTATA600.00832646513.2990311
GTTTTGG904.9019676E-513.2990311
GAATATA802.7039406E-413.299031
GTCCTAC3150.013.0879361
AGGACCT2500.012.8983325
GTATAGA854.511938E-412.5167351
CAAGACC600.00237817412.5063534
CTTACAC704.6483442E-412.4735643
GGGCTAC750.002515177212.412431
GTATAAT750.002515177212.412431
TTGGACA953.5303037E-612.4123214
Produced by FastQC (version 0.11.5)