##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926485_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 729234 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.203950995153818 26.0 2.0 30.0 2.0 31.0 2 15.89378855072583 16.0 2.0 30.0 2.0 31.0 3 19.38396317231506 22.0 12.0 30.0 2.0 31.0 4 21.516125139529972 27.0 2.0 35.0 2.0 37.0 5 21.87401026282373 28.0 2.0 35.0 2.0 35.0 6 22.7416494568273 30.0 2.0 35.0 2.0 37.0 7 22.57109377785457 30.0 2.0 35.0 2.0 37.0 8 22.644941130007652 30.0 2.0 35.0 2.0 37.0 9 25.242889113782407 31.0 17.0 37.0 2.0 39.0 10 25.86971945904881 31.0 17.0 37.0 2.0 39.0 11 23.083464292668747 29.0 7.0 37.0 2.0 39.0 12 24.13512535071047 30.0 7.0 37.0 2.0 39.0 13 20.92006680983059 27.0 2.0 35.0 2.0 39.0 14 24.103184985889303 30.0 8.0 37.0 2.0 40.0 15 25.132661121121615 31.0 8.0 38.0 2.0 40.0 16 25.492364590789787 31.0 7.0 38.0 2.0 40.0 17 24.78969439164932 30.0 2.0 38.0 2.0 40.0 18 24.82533590041057 30.0 2.0 38.0 2.0 40.0 19 25.218420973240413 31.0 2.0 38.0 2.0 40.0 20 25.432205574616653 31.0 2.0 38.0 2.0 40.0 21 23.357672571492827 30.0 2.0 37.0 2.0 40.0 22 24.24871029052403 30.0 2.0 37.0 2.0 40.0 23 24.21560020514677 30.0 2.0 38.0 2.0 40.0 24 23.356103802071765 30.0 2.0 38.0 2.0 40.0 25 23.145282584191083 30.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.624036589799488 1101 2 10.129414552670884 1101 3 7.030821378190126 1101 4 8.229651788497776 1101 5 8.07516108034094 1101 6 7.924239441367465 1101 7 7.709211639659102 1101 8 5.936962204375099 1101 9 6.096232215679624 1101 10 5.460364710215117 1101 11 8.129889722414749 1101 12 5.7968021609968154 1101 13 10.058217465313483 1101 14 7.598596270261648 1101 15 6.9200063553372395 1101 16 6.560126434123767 1101 17 2.0253598400754846 1101 18 4.512522916393536 1101 19 5.384920254881646 1101 20 3.5171825147135003 1101 21 0.3395140241443819 1101 22 4.347937987493253 1101 23 5.66806738340669 1101 24 7.011301602711679 1101 25 7.575123608623098 1102 1 5.416326489568185 1102 2 10.020128165281182 1102 3 6.5785695393924115 1102 4 7.938280369716953 1102 5 7.567889109614843 1102 6 6.571405061663388 1102 7 6.822960706492207 1102 8 6.91094428757075 1102 9 5.890380494369154 1102 10 5.387150577479009 1102 11 7.9413765479339595 1102 12 5.248443922815909 1102 13 9.568814569742507 1102 14 7.268649261902922 1102 15 5.253168965022596 1102 16 5.67686163501471 1102 17 6.351528287408158 1102 18 6.538228145799614 1102 19 5.101256488681258 1102 20 5.556951334089103 1102 21 4.464126989351023 1102 22 5.30364835696615 1102 23 5.972447454633333 1102 24 7.264631019803623 1102 25 7.0492042097661525 1103 1 4.259609712329134 1103 2 8.673507657633554 1103 3 5.973907519102351 1103 4 7.4057324711001975 1103 5 6.444487888798182 1103 6 6.39754077771228 1103 7 6.5259975107927986 1103 8 5.791385317493862 1103 9 4.909372939689916 1103 10 4.156405867125837 1103 11 4.905095193272395 1103 12 3.6400859303675404 1103 13 7.877365467070181 1103 14 6.0752823860782 1103 15 5.217230146039292 1103 16 4.979120724857186 1103 17 2.7479365387391397 1103 18 1.399579820841744 1103 19 3.6853249226776192 1103 20 3.9204920071534275 1103 21 6.137572246115646 1103 22 1.605021242402593 1103 23 3.6622326384415125 1103 24 5.271932758995007 1103 25 1.364043867108574 1104 1 5.184252472605966 1104 2 9.725602336445405 1104 3 6.709641243324562 1104 4 7.185774587141779 1104 5 6.830803527502276 1104 6 6.697850705456755 1104 7 6.7373785939247455 1104 8 6.621044518873759 1104 9 5.696856978563449 1104 10 4.394089667687183 1104 11 7.532741235674898 1104 12 4.630864894288539 1104 13 8.703741323790311 1104 14 6.4644858077780185 1104 15 5.787478911051892 1104 16 5.1872702703269695 1104 17 5.512669382242393 1104 18 3.461127143486582 1104 19 4.179899511040549 1104 20 3.3518626679364445 1104 21 2.434828608472074 1104 22 4.245822605231378 1104 23 4.748854547925543 1104 24 5.843659547174479 1104 25 4.88883412495505 1105 1 3.0749287542680683 1105 2 9.117786244766393 1105 3 6.171103004158329 1105 4 5.551221973334059 1105 5 1.8768296557100577 1105 6 4.718042315503933 1105 7 5.8441673700764305 1105 8 3.005932243903711 1105 9 4.055435855759914 1105 10 3.806876710412144 1105 11 6.753848248157606 1105 12 4.18452366283779 1105 13 4.72112583528159 1105 14 3.5233798480320395 1105 15 4.129727807921377 1105 16 4.557458560664582 1105 17 4.9086377594666715 1105 18 2.525351859711229 1105 19 2.215074675067097 1105 20 1.5803956613634185 1105 21 2.599679403976168 1105 22 4.095199307330212 1105 23 1.7130561159051219 1105 24 4.647337905000889 1105 25 5.381103388691319 1106 1 5.9442229252032135 1106 2 10.479845884623955 1106 3 7.355062233952719 1106 4 8.452132809891843 1106 5 8.076334303253294 1106 6 7.154881263313637 1106 7 6.961161260086982 1106 8 6.842034668143214 1106 9 5.8967670673014325 1106 10 5.063901632230298 1106 11 7.2570291289019835 1106 12 4.740142395641396 1106 13 3.7722271271492325 1106 14 5.295059348246468 1106 15 5.518401800471814 1106 16 5.613663356138947 1106 17 6.0841590119181355 1106 18 5.970062598933218 1106 19 5.75841921968496 1106 20 5.208831928102043 1106 21 5.55492120150808 1106 22 6.006461106885936 1106 23 6.440789950694477 1106 24 7.171636316307261 1106 25 6.340582545821167 1107 1 3.695223030477454 1107 2 -13.215029891773561 1107 3 -5.243731658227318 1107 4 1.7689735778195654 1107 5 4.3925015553085345 1107 6 4.617243330134066 1107 7 4.421830564989893 1107 8 5.148382704778648 1107 9 3.54872445037525 1107 10 2.929809766100849 1107 11 5.542852277750189 1107 12 3.007534178508493 1107 13 4.207746655520641 1107 14 3.5431727860246056 1107 15 3.3666238621242393 1107 16 3.1964986306654524 1107 17 3.81877461738857 1107 18 -0.9747150810139544 1107 19 1.7463150747510419 1107 20 1.9742067522462108 1107 21 -1.5560701468635187 1107 22 1.8198936213132946 1107 23 -1.3207579155881 1107 24 2.3864232795282696 1107 25 3.0496069407335504 1108 1 5.2613534749189945 1108 2 10.066388766669 1108 3 0.608638930294493 1108 4 5.920547139184954 1108 5 7.251003990108291 1108 6 7.323910844238561 1108 7 7.291734800555432 1108 8 6.931761558195269 1108 9 5.550365074168692 1108 10 4.7626324587434645 1108 11 6.608300217941668 1108 12 2.253069982954692 1108 13 3.345221663034721 1108 14 4.877747180175859 1108 15 4.657178217142871 1108 16 4.720038196310007 1108 17 0.12870639072350087 1108 18 3.512784815036312 1108 19 4.667177845658898 1108 20 4.876149483665049 1108 21 4.707766156660199 1108 22 1.6714201379993057 1108 23 4.441221548696554 1108 24 5.20449223799946 1108 25 5.758533431046029 1109 1 3.475419989008973 1109 2 -13.215029891773561 1109 3 -5.245855326747451 1109 4 1.8326099414559316 1109 5 1.3820372959128058 1109 6 2.5406237816648485 1109 7 3.515720830950901 1109 8 3.734468321446432 1109 9 3.503839342851972 1109 10 2.682888080564574 1109 11 0.8205102130352415 1109 12 1.2543151793793967 1109 13 2.588037403829073 1109 14 3.4001835718489524 1109 15 3.395095758687514 1109 16 3.187816377031101 1109 17 3.7352883173952662 1109 18 3.84701065088127 1109 19 2.364376306076828 1109 20 0.9299781731613308 1109 21 0.4719595978943012 1109 22 2.7749817167108937 1109 23 3.1476081325126586 1109 24 4.163632363738092 1109 25 4.654952958017667 1110 1 3.256320054366416 1110 2 8.942450423187069 1110 3 6.094607154968028 1110 4 7.465466061713624 1110 5 8.311694013724267 1110 6 4.281288560227608 1110 7 6.448163020736466 1110 8 7.228844469092007 1110 9 5.710599169810358 1110 10 5.3579900070530115 1110 11 3.3623856604225004 1110 12 5.761769040136922 1110 13 10.019984744403597 1110 14 7.641128453738716 1110 15 6.883767135239751 1110 16 6.570406399128139 1110 17 7.119941767334577 1110 18 7.062329190609258 1110 19 5.649505931672049 1110 20 6.108027409101702 1110 21 3.8214725293312313 1110 22 6.0569306134150125 1110 23 6.727309978082356 1110 24 7.626574693575321 1110 25 7.860137981826448 1111 1 4.57872408979949 1111 2 8.79903260822644 1111 3 6.941771278983776 1111 4 7.528845736910476 1111 5 7.99999987002348 1111 6 7.366352536605561 1111 7 6.9904120364845 1111 8 7.245072323422718 1111 9 5.673960390282801 1111 10 5.091589908627814 1111 11 6.8209363493988775 1111 12 6.156189561790466 1111 13 7.962818159757926 1111 14 5.88299607184895 1111 15 5.341062902956288 1111 16 5.1867757396793195 1111 17 6.521639601980247 1111 18 6.18401349179036 1111 19 5.0771449572626 1111 20 4.740421602015086 1111 21 7.471681682874539 1111 22 6.762657729556743 1111 23 6.666095657216214 1111 24 6.232234142394219 1111 25 6.911792755448495 1112 1 -1.5775737161586427 1112 2 7.7173372579848944 1112 3 6.045624690819523 1112 4 0.27468284439839863 1112 5 3.8254716393471497 1112 6 2.8408140905508112 1112 7 5.6834122377727425 1112 8 5.794415620524166 1112 9 4.461093039236101 1112 10 3.5655406936519682 1112 11 -0.3833837995544229 1112 12 3.872877615333138 1112 13 7.921180428276447 1112 14 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2204 9 2.8047850362308715 2204 10 2.499926110437414 2204 11 4.150972983938608 2204 12 4.486226811003682 2204 13 2.6658405092858573 2204 14 3.57736689206925 2204 15 3.6301352377320555 2204 16 3.6747299833850633 2204 17 -1.4373223374375321 2204 18 2.2044815041821764 2204 19 2.875892867372748 2204 20 3.185955384469846 2204 21 4.4276304928666725 2204 22 4.009985743915518 2204 23 2.8899099520627907 2204 24 4.480016400458734 2204 25 4.446850657690824 2205 1 5.247520856875468 2205 2 9.57391868842598 2205 3 6.471829917702117 2205 4 7.155040695659515 2205 5 7.346923435833148 2205 6 6.981219670143549 2205 7 7.0931053403985445 2205 8 6.48820475818864 2205 9 5.2727156665682955 2205 10 4.729613939709932 2205 11 6.680665100358201 2205 12 5.844602718927842 2205 13 9.159760912060307 2205 14 6.648533533475966 2205 15 5.516090483539557 2205 16 5.355244537645554 2205 17 6.065200135364744 2205 18 5.863015674253905 2205 19 4.411092458221923 2205 20 4.72700904445561 2205 21 -0.9720167534263027 2205 22 3.2886967980525235 2205 23 4.455576063010536 2205 24 6.225888209930673 2205 25 6.516691906446194 2206 1 3.8048058205687205 2206 2 8.080354723611055 2206 3 5.643922721291467 2206 4 1.7119106407566278 2206 5 5.269459004638865 2206 6 5.446400613528638 2206 7 5.593729344176808 2206 8 5.47831751573041 2206 9 4.351604621052029 2206 10 3.561854331704737 2206 11 3.0837969263219556 2206 12 4.073749657944312 2206 13 7.24496960206562 2206 14 0.2263374180027995 2206 15 1.9645484798793653 2206 16 2.934912758910091 2206 17 3.874956909672555 2206 18 -0.9590033351327172 2206 19 0.9265319764933686 2206 20 2.169099486630472 2206 21 2.4551672597976157 2206 22 -2.2776908281355617 2206 23 -2.9542769389376318 2206 24 0.6217533731634468 2206 25 2.2334874669869578 2207 1 1.1886495439859033 2207 2 -13.215029891773561 2207 3 -5.888448162169464 2207 4 -3.9475279301511357 2207 5 1.2126319296601302 2207 6 1.683221612434945 2207 7 2.9554051249521116 2207 8 3.093371592775007 2207 9 0.6309459748009694 2207 10 0.35988325380948893 2207 11 -1.8526048235869048 2207 12 -2.4761008706739887 2207 13 0.0961848165509771 2207 14 -5.0759617678034985 2207 15 -1.4997521641843115 2207 16 -0.47254865803 2207 17 0.6579597046658705 2207 18 0.5925800893417019 2207 19 0.18508951689925013 2207 20 -0.041408473024407755 2207 21 -3.498991204171272 2207 22 -4.343805787425879 2207 23 -1.3861641670333888 2207 24 0.32096834460590884 2207 25 0.8262933972336448 2208 1 5.03625145366998 2208 2 6.577464589462643 2208 3 6.408951305657801 2208 4 8.187630344158453 2208 5 3.8221283882353987 2208 6 6.427829265818218 2208 7 6.971342408081259 2208 8 7.083934768235078 2208 9 5.491475566883242 2208 10 4.899321926655777 2208 11 7.024055600686587 2208 12 6.64987690082652 2208 13 8.941661173922753 2208 14 3.123362379795971 2208 15 4.770752702441204 2208 16 5.384225036036938 2208 17 6.110259451134038 2208 18 0.9321802274415738 2208 19 3.7750863433921076 2208 20 4.617324425782563 2208 21 7.274734249099705 2208 22 6.342908028489784 2208 23 2.506667194191934 2208 24 4.088290985661327 2208 25 5.388726047391099 2209 1 3.281179446942346 2209 2 4.759067439466627 2209 3 5.4286322974296155 2209 4 1.2055370143830046 2209 5 5.191923682817826 2209 6 5.714689467531777 2209 7 5.770636722512755 2209 8 4.41213227240231 2209 9 4.4208353902828 2209 10 3.367097659805207 2209 11 -0.07625016943942597 2209 12 3.3356648855738236 2209 13 2.4262097904486666 2209 14 3.8694143410797217 2209 15 4.321393063866807 2209 16 3.5989629943888986 2209 17 1.4487245705830745 2209 18 3.4341520808798407 2209 19 4.081963687639366 2209 20 4.252600271559828 2209 21 6.529248892450678 2209 22 4.996751872021424 2209 23 0.6516885241706873 2209 24 -2.7157348528962046 2209 25 0.6174749081172557 2210 1 -1.9969311521359963 2210 2 -13.215029891773561 2210 3 -7.378062390371063 2210 4 -2.1767742227669444 2210 5 -0.7117692832023259 2210 6 -1.5119740762976654 2210 7 -1.110898447386468 2210 8 -19.893990176577283 2210 9 -12.517874287136555 2210 10 -6.849287107501219 2210 11 -9.256029376407573 2210 12 -4.412888059177277 2210 13 -4.887660670887234 2210 14 -3.7457841700865337 2210 15 -5.5170520163985515 2210 16 -4.857705107094873 2210 17 -4.339348301245558 2210 18 -4.637886709822542 2210 19 -9.697376212092239 2210 20 -6.872128801210721 2210 21 -5.057400978415782 2210 22 -9.484998520443256 2210 23 -7.836348899235398 2210 24 -10.506273922121911 2210 25 -8.292567123583764 2211 1 0.11276207999556576 2211 2 4.494120435023824 2211 3 0.4448503231014236 2211 4 1.3790448790673366 2211 5 0.945889371657465 2211 6 -0.07186233267548658 2211 7 -19.8439629635155 2211 8 -12.884186255008656 2211 9 -9.021975067233539 2211 10 -6.957725859346041 2211 11 -9.216492163673017 2211 12 -4.607632946379468 2211 13 -6.424135475862988 2211 14 -4.446385055602029 2211 15 -4.919466100311688 2211 16 -11.03448140901349 2211 17 -7.4331602346210595 2211 18 -5.356034506575654 2211 19 -8.240966766593612 2211 20 -6.504012630011058 2211 21 -4.166788496864022 2211 22 -5.566697866848486 2211 23 -7.562371458796857 2211 24 -20.649015857605782 2211 25 -20.428050994551505 2212 1 -1.56077353678279 2212 2 -13.215029891773561 2212 3 -7.7734482621554655 2212 4 -18.821935513089525 2212 5 -19.08515637997652 2212 6 -19.93052246339444 2212 7 -9.866747773642084 2212 8 -9.407914227210194 2212 9 -5.622202584400743 2212 10 -5.924766895169654 2212 11 -5.609792817267788 2212 12 -3.1528261291378037 2212 13 -6.2973380902420715 2212 14 -4.158466217180585 2212 15 -4.1971425295331635 2212 16 -4.25963446601688 2212 17 -4.290161558357305 2212 18 -3.4114473468172335 2212 19 -3.8776776956909877 2212 20 -4.163659357900524 2212 21 -1.988886618813222 2212 22 -3.3585217271943115 2212 23 -3.350739126539061 2212 24 -2.3966951825002987 2212 25 -2.8643379143827303 2213 1 -17.57596341020051 2213 2 -13.215029891773561 2213 3 -8.45032567182485 2213 4 -5.77791035585682 2213 5 -19.08515637997652 2213 6 -10.625041780555266 2213 7 -19.8439629635155 2213 8 -10.31447535177944 2213 9 -8.240440440804353 2213 10 -7.291537056780992 2213 11 -6.438364245839217 2213 12 -5.216105895307468 2213 13 -18.097338090242072 2213 14 -11.865225233182494 2213 15 -7.106813051670848 2213 16 -6.2476154047501495 2213 17 -4.825808174300075 2213 18 -4.856869117149444 2213 19 -5.576759888873969 2213 20 -5.612811495469192 2213 21 -3.8721511450679564 2213 22 -4.882235717208754 2213 23 -4.600935312011099 2213 24 -3.6139754263389356 2213 25 -4.547547849897416 2214 1 -17.57596341020051 2214 2 -13.215029891773561 2214 3 -12.276979510955558 2214 4 -18.821935513089525 2214 5 -19.08515637997652 2214 6 -19.93052246339444 2214 7 -16.240322923070607 2214 8 -14.83028946879164 2214 9 -14.275827905976048 2214 10 -14.10269882999706 2214 11 -14.449293996910527 2214 12 -12.64047294452905 2214 13 -18.097338090242072 2214 14 -16.293648581841644 2214 15 -22.482374597043712 2214 16 -17.738261387469315 2214 17 -22.162735398019752 2214 18 -17.190290571657567 2214 19 -14.839190198197462 2214 20 -13.756537131554175 2214 21 -11.337195655345885 2214 22 -12.321828651889161 2214 23 -12.216818744957697 2214 24 -11.196033046685661 2214 25 -10.923500943995387 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 149158.0 3 1.0 4 849.0 5 2969.0 6 3575.0 7 3512.0 8 3101.0 9 2987.0 10 2839.0 11 2617.0 12 2524.0 13 2576.0 14 3864.0 15 7237.0 16 10263.0 17 11131.0 18 11642.0 19 13579.0 20 16323.0 21 18992.0 22 20134.0 23 21581.0 24 22620.0 25 23030.0 26 19666.0 27 16088.0 28 17139.0 29 19475.0 30 19316.0 31 21721.0 32 27709.0 33 33466.0 34 39833.0 35 43896.0 36 43009.0 37 43460.0 38 27352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57874083324017 18.051804692310235 12.208541522646641 26.160912951802963 2 14.371233152111145 20.21297528049516 40.219990332915565 25.195801234478125 3 18.682282979186308 24.28588004931467 29.30466313728334 27.72717383421568 4 11.256105839294785 16.446959303898716 38.579885027461444 33.71704982934505 5 13.042140831206364 37.3161051359631 36.2953170894842 13.346436943346344 6 32.14074904701499 37.24311795258989 18.103115937044603 12.51301706335051 7 27.840035289887695 30.952222688859816 22.949606161227653 18.258135860024836 8 25.042395411076274 36.602531728059176 20.564403257744846 17.79066960311971 9 26.068305673379182 15.401309558127085 19.76320882922209 38.767175939271645 10 13.487038751671768 29.816015059127093 35.31957547497699 21.37737071422415 11 33.51147164293423 22.36027457670392 24.369697919205283 19.758555861156566 12 23.008644027202195 25.035599457117353 31.753119035004318 20.202637480676135 13 28.09449494055497 21.090358716357656 25.331003493136333 25.484142849951034 14 20.284294206913835 21.82090300304899 28.404360477257857 29.49044231277932 15 22.78923803516674 30.639878873569348 23.55573240992774 23.015150681336173 16 22.035835266264165 28.567917209347005 27.213641359163503 22.182606165225327 17 21.27932990839561 28.103391365432955 28.743521817756072 21.87375690841536 18 21.27551247523871 27.707327116116538 29.874913752809988 21.14224665583476 19 22.431474946678794 27.279464313025393 27.629980006284534 22.659080734011283 20 22.71350854804286 27.703717353517128 28.170311778036922 21.41246232040309 21 22.018146493881066 26.30111391202584 27.108864656684474 24.571874937408616 22 21.970262397166042 27.826715384572537 28.264229657396527 21.938792560864893 23 21.912462164629336 27.557602421463724 28.116158877670784 22.41377653623616 24 21.950475606659545 27.4598803132949 28.282482259593642 22.307161820451913 25 22.169629362191074 27.320175661372758 28.20479732911248 22.30539764732369 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 657.0 1 657.0 2 1430.0 3 2203.0 4 2203.0 5 2203.0 6 3131.5 7 4060.0 8 4060.0 9 4060.0 10 4850.0 11 5640.0 12 5640.0 13 5640.0 14 6862.0 15 8084.0 16 8084.0 17 8084.0 18 11575.0 19 15066.0 20 15066.0 21 15066.0 22 20917.5 23 26769.0 24 26769.0 25 26769.0 26 35176.5 27 43584.0 28 43584.0 29 43584.0 30 52739.0 31 61894.0 32 61894.0 33 61894.0 34 69650.0 35 77406.0 36 77406.0 37 77406.0 38 83225.5 39 89045.0 40 89045.0 41 89045.0 42 90848.0 43 92651.0 44 92651.0 45 92651.0 46 91410.0 47 90169.0 48 90169.0 49 90169.0 50 83440.5 51 76712.0 52 76712.0 53 76712.0 54 66787.5 55 56863.0 56 56863.0 57 56863.0 58 47426.5 59 37990.0 60 37990.0 61 37990.0 62 29942.0 63 21894.0 64 21894.0 65 21894.0 66 16478.5 67 11063.0 68 11063.0 69 11063.0 70 7896.0 71 4729.0 72 4729.0 73 4729.0 74 3279.5 75 1830.0 76 1830.0 77 1830.0 78 1237.5 79 645.0 80 645.0 81 645.0 82 424.5 83 204.0 84 204.0 85 204.0 86 130.0 87 56.0 88 56.0 89 56.0 90 36.0 91 16.0 92 16.0 93 16.0 94 8.5 95 1.0 96 1.0 97 1.0 98 2.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.916978637858357 2 35.88258364256192 3 5.455587644020986 4 13.336871292342375 5 13.070153064722708 6 14.274293299544452 7 9.227216503893127 8 7.410927082390563 9 0.10202486444680309 10 1.3625256090637572 11 5.4690264030475815 12 5.528952297890664 13 15.558106177166724 14 1.5034954486488563 15 2.818436880342935 16 2.924712780808355 17 2.7859370243296393 18 1.4220401133243925 19 1.3723989830424803 20 0.2811168980053042 21 4.149011154169992 22 1.5202253323350254 23 0.10271051541754772 24 8.73011955010326 25 0.10792146279520703 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 729234.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.79151199108263 #Duplication Level Percentage of deduplicated Percentage of total 1 97.70428687249266 93.59241367526559 2 1.7846378235080118 3.4190631094061468 3 0.25806232133038354 0.7416053984449815 4 0.09704221688585457 0.3718328272985032 5 0.043840638424395874 0.20997805206636175 6 0.028591558258065076 0.1643297157432707 7 0.01726576067928255 0.11577393248012602 8 0.011951348875155596 0.09158702232672633 9 0.007196953530387485 0.06204663543648395 >10 0.04320770124643569 0.7441848549252519 >50 0.001885979623305267 0.1227080406155635 >100 0.002030825146013347 0.3644767359909178 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3713019414892888E-4 2 0.0 0.0 0.0 0.0 1.3713019414892888E-4 3 0.0 0.0 0.0 0.0 1.3713019414892888E-4 4 0.0 0.0 0.0 0.0 1.3713019414892888E-4 5 0.0 0.0 0.0 0.0 1.3713019414892888E-4 6 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 7 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 8 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 9 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 10 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 11 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 12 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 13 1.3713019414892888E-4 0.0 0.0 0.0 1.3713019414892888E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAACCC 40 0.0023600028 16.079597 4 ATTAACC 55 5.998782E-5 15.875445 3 GTCTAAA 70 8.6950866E-5 15.198893 1 TAGGACC 185 0.0 14.486124 4 TAGGACG 130 2.5465852E-11 14.018112 4 GTAGGAC 265 0.0 14.003341 3 TGTAGGA 285 0.0 13.860546 2 GAAGTGC 60 1.9097739E-4 13.857509 6 TCTTGGA 60 0.007105877 13.621571 2 GAGTATA 60 0.008326465 13.299031 1 GTTTTGG 90 4.9019676E-5 13.299031 1 GAATATA 80 2.7039406E-4 13.29903 1 GTCCTAC 315 0.0 13.087936 1 AGGACCT 250 0.0 12.898332 5 GTATAGA 85 4.511938E-4 12.516735 1 CAAGACC 60 0.002378174 12.506353 4 CTTACAC 70 4.6483442E-4 12.473564 3 GGGCTAC 75 0.0025151772 12.41243 1 GTATAAT 75 0.0025151772 12.41243 1 TTGGACA 95 3.5303037E-6 12.412321 4 >>END_MODULE