FastQCFastQC Report
Thu 26 May 2016
SRR1926485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729234
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT9130.12519986725797208No Hit
CCCATGTACTCTGCGTTGATACCAC7560.10367042677659023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2400.014.7022354
GTAGGAC5150.014.0763223
TGTAGGA5600.013.6293362
CTAAGAC1001.3643694E-712.4001273
GCCTAGG701.0502772E-412.262171
CTGTAGG5550.012.2007971
AGGACGT3550.012.0886285
TAGGACG3400.012.0609754
TAGAGCT1052.58E-711.80724
GTCCTAA1700.011.7813011
TATGGCT751.9982713E-411.4439014
AGACAAT751.9982713E-411.4439016
AGGACCT3700.011.3408035
GGCGAGA2350.011.28793719
GGACGTG3650.011.2348816
CTGTTAG855.105912E-511.2202861
CCTACTC600.00569614711.128323
GGACCTG3350.011.1022926
TGTGCGA600.00595282611.06263410
GACGTGG2150.011.0448337
Produced by FastQC (version 0.11.5)